Application of circRNA PRKD3 to osteogenic differentiation of periodontal ligament stem cells

A technology of periodontal ligament stem cells and osteogenic differentiation, applied in the direction of DNA/RNA fragments, recombinant DNA technology, biochemical equipment and methods, etc., can solve the problems of inability to achieve regeneration

Active Publication Date: 2019-09-17
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, with these treatments alone or in combination, ...

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  • Application of circRNA PRKD3 to osteogenic differentiation of periodontal ligament stem cells
  • Application of circRNA PRKD3 to osteogenic differentiation of periodontal ligament stem cells
  • Application of circRNA PRKD3 to osteogenic differentiation of periodontal ligament stem cells

Examples

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Embodiment 1

[0088] 1. In vitro isolation, culture and identification of human periodontal ligament stem cells

[0089] 1. Cell Culture

[0090] 1.1 Primary cell culture

[0091] In this example, the tissue block method was used to isolate and culture human periodontal ligament stem cells (hPDLSCs), and the subjects were 12-20-year-old patients without systemic diseases and oral diseases. After obtaining the informed consent of the patients and their families, the freshly extracted healthy and complete orthodontic teeth or third molars were placed in a centrifuge tube containing 5% double-antibody PBS. Rinse the blood stains on the root surface with 2% double-antibody PBS in the ultra-clean workbench, and then use a disposable sterile blade to scrape the periodontal ligament of the 1 / 3 part of the root surface to the 1% double-antibody PBS 8cm 2 In the cell culture dish, the size of the tissue block is about 4-9mm2, wash the tissue block 3-4 times with PBS containing 1% double antibod...

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Abstract

The disclose belongs to the field of osteogenic differentiation of periodontal ligament stem cells, and particularly relates to an application of circRNA PRKD3 to osteogenic differentiation of periodontal ligament stem cells. In order to define the osteogenic differentiation mechanism of the periodontal ligament stem cells, the inventor firstly researches and inspires that specific lncRNA and circRNA are possibly used as ceRNA to adjust the osteogenic differentiation of the periodontal ligament stem cells and influence periodontal regeneration. In order to further confirm the relationship between the circRNA and the osteogenic differentiation of the periodontal ligament stem cells, circRNA pertinent to miRNA-21 is forecast by a bioinformatics method, the situation that circRNA PRKD3 has the adjustment effect during the osteogenic differentiation of the periodontal ligament stem cells is confirmed through screening, expression of osteogenesis key genes in hPDLSCs can be induced, the number of mineralization nodes in cells can be increased, and hPDLSCs osteogenesis induced disintegration is promoted.

Description

technical field [0001] The disclosure belongs to the field of osteogenic differentiation of periodontal ligament stem cells, and in particular relates to an induction effect of circRNA PRKD3 on osteogenic differentiation of periodontal ligament stem cells. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] Periodontal disease is the main cause of tooth loss in adults. For patients with periodontal tissue loss, it is difficult to regenerate the missing tissue. The current periodontal guided tissue regeneration (guided tissue regeneration, GTR), periodontal bone grafting or implantation of bone substitutes, and the use of bioactive drugs such as growth factors are the main ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6883C12Q2600/158C12Q2600/178C12Q2561/113C12Q2563/107C12Q2545/114C12Q2521/107
Inventor 郭杰魏福兰靳也王红张子杰
Owner SHANDONG UNIV
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