Wrinkle-removing and anti-aging composition containing vermilion as well as preparation method and application of wrinkle-removing and anti-aging composition

Abstract

The invention provides an anti-wrinkle and anti-aging composition containing vermilion as well as a preparation method and application of the anti-wrinkle and anti-aging composition, and relates to the technical field of cosmetics. The anti-wrinkle and anti-aging composition is prepared from the following components in parts by weight: 0.5 to 2 parts of a water-soluble active component, 10 to 14 parts of an oil-soluble active component and 85 to 89 parts of a nano wrapping material, the water-soluble active ingredients are selected from at least two of carnosine, theanine and VC ethyl ether; the oil-soluble active ingredients are selected from at least three of millettiamide, quercus roei seed oil, bakuchiol, tocopheryl acetate and phytol; the nano wrapping material is selected from at least four of glycerol, 1, 3-propylene glycol, butanediol, water, caprylic / capric triglyceride, lecithin and hydrogenated lecithin; all the components interact with one another, so that the transdermal absorption effect is improved, and the long-acting wrinkle-removing and anti-aging effects are achieved; the anti-wrinkle and anti-aging composition is applied to preparation of cosmetics, and is friendly to sensitive skin and high in safety.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle and anti-aging composition containing acmella oleracea amide, and a preparation method and application thereof. Background Art

[0002] Acmella oleracea amide is an active ingredient in acmella oleracea extract, which has effects such as anti-aging, anti-wrinkle and anti-stria. However, due to the generally poor transdermal absorption characteristics of acmella oleracea amide, it is difficult to effectively penetrate the skin barrier; the effect is weak in the long-term anti-aging process, resulting in limitations in its application and effect exertion in cosmetics.

[0003] Liposomes have a unique closed structure. The liposome membrane can encapsulate hydrophobic active ingredients and entrap hydrophilic active ingredients in the lumen, promoting the transdermal absorption of active ingredients. As a delivery carrier, liposomes can effectively control the local concentration of active ingredients and improve the permeability of active ingredients. Therefore, in the preparation of cosmetics, liposomes are often added to improve the absorption of active ingredients by the skin, so as to fully exert the effect.

[0004] Chinese Patent CN109528545A discloses an anti-wrinkle and anti-aging active polypeptide, which is encapsulated in a nano-inclusion by lipid components; the lipid components are selected from one or more of soybean lecithin, egg yolk lecithin, phosphatidylethanolamine, lysophosphatidylcholine, cholesterol, lauric acid, myristic acid, palmitic acid, stearic acid, isopropyl myristate, isopropyl palmitate, oleic acid, glyceryl linoleate, vitamin E, and the mass percentage concentration of each component is 2%-20%; the polypeptide is selected from one or more of tripeptide-1, acetyl hexapeptide-8, acetyl octapeptide-3, β-alanyl hydroxyprolyl diaminobutyryl benzylamide, palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7, carnosine, tripeptide-1 copper, palmitoyl tripeptide-1, palmitoyl tripeptide-5, palmitoyl oligopeptide, hexapeptide-9, hexapeptide-11. The product of this invention has good stability, and the lipid components are easy to be absorbed through the skin.

[0005] Chinese Patent CN114632045A discloses an anti-aging composition, a preparation method and application thereof, including nano-liposomes and active components loaded on the nano-liposomes. The active components include diglucosyl gallic acid, glucosyl hesperidin, acmella oleracea extract, biotin and caffeine; this anti-aging composition can promote the proliferation of human skin fibroblasts, promote the production of collagen, improve the transdermal absorption ability of the anti-aging composition, thereby improving wrinkles, improving skin color, repairing damaged skin, and improving skin dryness.

[0006] However, traditional cosmetic products containing active ingredients and liposomes often face the problem that multiple active ingredients cannot ensure rapid and long-lasting efficacy. Therefore, it is necessary to study the combination of multiple active ingredients and the use of specific liposomes to improve the long-term anti-aging and anti-wrinkle effects of the composition. Summary of the Invention

[0007] In view of the problems existing in the prior art, the present invention provides an anti-wrinkle and anti-aging composition containing zinnamide, its preparation method and application. By mixing water-soluble active ingredients, oil-soluble active ingredients and nano-encapsulation materials, the components interact with each other to improve the transdermal absorption effect and bring long-term anti-wrinkle and anti-aging effects. The composition of the present invention is applied to the preparation of cosmetics, is friendly to sensitive skin and has high safety.

[0008] To achieve the above object, the technical scheme adopted by the present invention is as follows:

[0009] First of all, the present invention provides an anti-wrinkle and anti-aging composition containing zinnamide, which is composed of the following components according to weight: 0.5-2 parts of water-soluble active ingredients, 10-14 parts of oil-soluble active ingredients and 85-89 parts of nano-encapsulation materials;

[0010] The water-soluble active ingredients are selected from at least 2 of carnosine, L-theanine, and VC ethyl ether;

[0011] The oil-soluble active ingredients are selected from at least 3 of zinnamide, Rosa roxburghii Tratt seed oil, bakuchiol, tocopheryl acetate, and phytol;

[0012] The nano-encapsulation materials are selected from at least 4 of glycerol, 1,3-propanediol, butanediol, water, caprylic / capric triglyceride, lecithin, and hydrogenated lecithin.

[0013] Preferably, the weight ratio of the water-soluble active ingredients, oil-soluble active ingredients and nano-encapsulation materials is 1:10:89.

[0014] Preferably, the water-soluble active ingredient is a mixture of carnosine and L-theanine; or a mixture of carnosine and VC ethyl ether.

[0015] Further preferably, when the water-soluble ingredient is a mixture of carnosine and L-theanine, the weight ratio of carnosine to L-theanine is 0.5-2:1.

[0016] Even more preferably, the weight ratio of carnosine to L-theanine is 1:1.

[0017] Further preferably, when the water-soluble active ingredient is a mixture of carnosine and VC ethyl ether, the weight ratio of carnosine to VC ethyl ether is 1-2:1-3.

[0018] More preferably, the weight ratio of carnosine to VC ethyl ether is 1:1 - 1.5.

[0019] Preferably, the oil-soluble active ingredient is a mixture of zafirlukast amide, tocopheryl acetate, and a third oil-soluble active ingredient; the third oil-soluble active ingredient is selected from one of phytol, bakuchiol, and Rosa roxburghii Tratt seed oil.

[0020] More preferably, the weight ratio of zafirlukast amide, tocopheryl acetate, and the third oil-soluble active ingredient is 6 - 9:1 - 4:1 - 4.

[0021] Even more preferably, the weight ratio of zafirlukast amide, tocopheryl acetate, and the third oil-soluble active ingredient is 6:3:1.

[0022] Preferably, the nano-encapsulated material is a mixture of a first nano-encapsulated material, a second nano-encapsulated material, a third nano-encapsulated material, and a fourth nano-encapsulated material; the first nano-encapsulated material is selected from at least one of glycerol, 1,3-propanediol, and butanediol; the second nano-encapsulated material is water; the third nano-encapsulated material is glyceryl caprylate / caprate; the fourth nano-encapsulated material is lecithin or hydrogenated lecithin.

[0023] More preferably, in the nano-encapsulated material, the mass ratio of the first nano-encapsulated material, the second nano-encapsulated material, the third nano-encapsulated material, and the fourth nano-encapsulated material is 40 - 49:15 - 22.5:14 - 20:5.

[0024] Even more preferably, in the nano-encapsulated material, the mass ratio of the first nano-encapsulated material, the second nano-encapsulated material, the third nano-encapsulated material, and the fourth nano-encapsulated material is 49:20:15:5.

[0025] Then, the present invention provides a preparation method of the above anti-wrinkle and anti-aging composition, including the steps:

[0026] (1) Mix the components of the nano-encapsulated material and shear to obtain mixture 1;

[0027] (2) Mix mixture 1 from step (1) with the water-soluble active ingredient to obtain mixture 2;

[0028] (3) Mix mixture 2 from step (2) with the oil-soluble active ingredient and shear to obtain the anti-wrinkle and anti-aging composition.

[0029] Preferably, in step (1), the shear is specifically: heat to 50 - 70°C and shear at a rotation speed of 10000 - 12000 rpm.

[0030] Further preferably, in step (1), the shearing is specifically: heating to 60°C and shearing at a rotational speed of 11,000 rpm.

[0031] Preferably, in step (2), the mixing is specifically: heating to 50 - 70°C and mixing and stirring evenly at a rotational speed of 100 - 300 rpm.

[0032] Further preferably, in step (2), the mixing is specifically: heating to 60°C and mixing and stirring evenly at a rotational speed of 200 rpm.

[0033] Preferably, in step (3), the shearing is specifically: heating to 50 - 70°C and shearing at a rotational speed of 12,000 - 18,000 rpm for 3 - 5 min.

[0034] Further preferably, in step (3), the shearing is specifically: heating to 60°C and shearing at a rotational speed of 15,000 rpm for 3 min.

[0035] Preferably, in the preparation method of the anti-wrinkle and anti-aging composition, in order to obtain an anti-wrinkle and anti-aging composition with a more uniform texture, after step (3), further high-pressure homogenization and / or microfluidic homogenization treatment can be carried out.

[0036] Further preferably, the pressure of the high-pressure homogenization treatment is 8,000 - 12,000 psi.

[0037] Even more preferably, the pressure of the high-pressure homogenization treatment is 10,000 psi.

[0038] Further preferably, the pressure of the microfluidic homogenization treatment is 20,000 - 30,000 psi, and the number of treatment times is 2 - 3 times.

[0039] Even more preferably, the pressure of the microfluidic homogenization treatment is 25,000 psi, and the number of treatment times is 2 times.

[0040] Furthermore, the present invention provides the application of the above anti-wrinkle and anti-aging composition in cosmetics.

[0041] Preferably, the cosmetics in the application have the effects of anti-wrinkle and anti-aging.

[0042] Finally, the present invention provides a cosmetic with the effects of anti-wrinkle and anti-aging, comprising the component: the above anti-wrinkle and anti-aging composition.

[0043] Preferably, the cosmetics are not limited to toner, lotion, essence, cream, facial cleanser.

[0044] Preferably, in the cosmetics, the addition amount of the anti-wrinkle and anti-aging composition is 0.01% - 10%.

[0045] Further preferably, in the cosmetic, the addition amount of the anti-wrinkle and anti-aging composition is 1% - 5%.

[0046] Preferably, the cosmetic further comprises components: polyacrylate cross-linked polymer and butanediol.

[0047] Compared with the prior art, the present invention has the following beneficial effects:

[0048] 1. In the anti-wrinkle and anti-aging composition of the present invention, ambruticin and other water-soluble active ingredients and oil-soluble active ingredients interact and cooperate synergistically, bringing remarkable effects of anti-wrinkle and increasing the collagen content.

[0049] 2. In the anti-wrinkle and anti-aging composition of the present invention, a nano-encapsulating material with specific components and ratios is used to encapsulate the water-soluble active ingredients and oil-soluble active ingredients. When acting on the skin, it promotes the absorption of the active ingredients, thereby further exerting the anti-wrinkle and anti-aging effects of ingredients such as ambruticin.

[0050] 3. The anti-wrinkle and anti-aging composition of the present invention is obtained by a preparation method of step-by-step mixing and homogenization. The composition has a uniform texture, high stability, and is easily absorbed.

[0051] 4. The anti-wrinkle and anti-aging composition of the present invention is applied to the preparation of cosmetics, with high safety and being friendly to sensitive skin. Specific Embodiments

[0052] The following non-limiting examples can enable those of ordinary skill in the art to more comprehensively understand the present invention, but do not limit the present invention in any way. The following content is only an exemplary illustration of the scope claimed by the present invention. Those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, and it should also fall within the scope claimed by the present invention.

[0053] When the examples give a numerical range, it should be understood that unless otherwise specified in the present invention, any value at both ends of each numerical range and any value between the two ends can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention belongs.

[0054] The present invention will be further described below by way of specific examples. All kinds of chemical reagents used in the examples of the present invention are obtained through conventional commercial channels unless otherwise specified.

[0055] In the following examples, the carnosine was purchased from Emeishan Hongsheng Pharmaceutical Co., Ltd.; the theanine was purchased from Weiyitai Biotechnology Co., Ltd.; the VC ethyl ether was purchased from Baiyoujia Biotechnology Co., Ltd.; the centaureamide was purchased from Guangzhou Dipp Biotechnology Co., Ltd.; the roselle quercus fruit seed oil was purchased from Yusheng Biotechnology (International) Group; the bakuchiol was purchased from Yunnan Hengjin Pharmaceutical Co., Ltd.; the tocopheryl acetate was purchased from BASF Group; the phytol was purchased from Shenzhen Dickmann Biotechnology Co., Ltd.; the caprylic / capric triglyceride was purchased from Yihai (Lianyungang) Special Oils Co., Ltd.; the lecithin was purchased from Weiyue (Jiangmen) Biotechnology Co., Ltd.; the hydrogenated lecithin was purchased from Swiss Libode Co., Ltd.; the microfluidic homogenizer was purchased from Nozzle Fluid Technology (Shanghai) Co., Ltd., model Mini. Products from different manufacturers have no significant effect on the results.

[0056] Example 1

[0057] A wrinkle-removing and anti-aging composition containing centaureamide, with the ingredients by weight being: 1 part of water-soluble active ingredient, 10 parts of oil-soluble active ingredient, and 89 parts of nano-encapsulated material;

[0058] Among them, the water-soluble active ingredient is: 0.5 part of carnosine and 0.5 part of theanine;

[0059] The oil-soluble active ingredient is: 6 parts of centaureamide, 3 parts of tocopheryl acetate, and 1 part of phytol;

[0060] The nano-encapsulated material is: 49 parts of glycerol, 20 parts of water, 15 parts of caprylic / capric triglyceride, and 5 parts of lecithin.

[0061] The preparation method of the wrinkle-removing and anti-aging composition is as follows:

[0062] (1) Mix glycerol, water, caprylic / capric triglyceride, and lecithin, add them to a homogenization reaction kettle, heat to 60 °C, and shear and stir evenly at 11000 rpm to obtain mixture 1;

[0063] (2) Add carnosine and theanine to mixture 1, heat to 60 °C, and mix and stir evenly at 200 rpm to obtain mixture 2;

[0064] (3) Add centaureamide, tocopheryl acetate, and phytol to mixture 2, heat to 60 °C, and mix and shear at 15000 rpm for 3 min to make a uniform primary emulsion;

[0065] (4) Perform a high-pressure homogenization treatment on the primary emulsion in step (3) at 10000 psi once, and then perform a microfluidic homogenization treatment at a pressure of 25000 psi twice to obtain the wrinkle-removing and anti-aging composition containing centaureamide.

[0066] Example 2

[0067] An anti-wrinkle and anti-aging composition containing acmellinamide, with the ingredients by weight being: 2 parts of water-soluble active ingredients, 13 parts of oil-soluble active ingredients, and 85 parts of nano-encapsulated materials;

[0068] Among them, the water-soluble active ingredients are: 1 part of carnosine and 1 part of L-theanine;

[0069] The oil-soluble active ingredients are: 8 parts of acmellinamide, 4 parts of tocopheryl acetate, and 1 part of phytol;

[0070] The nano-encapsulated materials are: 45 parts of 1,3-propanediol, 15 parts of water, 20 parts of caprylic / capric triglyceride, and 5 parts of hydrogenated lecithin.

[0071] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0072] (1) Mix 1,3-propanediol, water, caprylic / capric triglyceride, and hydrogenated lecithin, add them to a homogenization reactor, heat to 55 °C, and shear and stir evenly at 10000 rpm to obtain mixture 1;

[0073] (2) Add carnosine and L-theanine to mixture 1, heat to 55 °C, and mix and stir evenly at 100 rpm to obtain mixture 2;

[0074] (3) Add acmellinamide, tocopheryl acetate, and phytol to mixture 2, heat to 55 °C, and mix and shear at 12000 rpm for 3 min to prepare a uniform primary emulsion;

[0075] (4) Perform a high-pressure homogenization treatment on the primary emulsion in step (3) at 10000 psi once, and then perform a microfluidic homogenization treatment at a pressure of 25000 psi twice to obtain the anti-wrinkle and anti-aging composition containing acmellinamide.

[0076] Example 3

[0077] An anti-wrinkle and anti-aging composition containing acmellinamide, with the ingredients by weight being: 0.5 part of water-soluble active ingredients, 14 parts of oil-soluble active ingredients, and 85.5 parts of nano-encapsulated materials;

[0078] Among them, the water-soluble active ingredients are: 0.2 part of carnosine and 0.3 part of VC ethyl ether;

[0079] The oil-soluble active ingredients are: 9 parts of acmellinamide, 4 parts of bakuchiol, and 1 part of tocopheryl acetate;

[0080] The nano-encapsulated materials are: 40 parts of glycerol, 22.5 parts of water, 18 parts of caprylic / capric triglyceride, and 5 parts of lecithin.

[0081] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0082] (1) Mix glycerin, water, triglyceride caprylate / caprate, and lecithin, add them to a homogenization reactor, heat to 65 °C, and shear and stir evenly at 10,000 rpm to obtain Mixture 1;

[0083] (2) Add carnosine and VC ethyl ether to Mixture 1, heat to 65 °C, and mix and stir evenly at 100 rpm to obtain Mixture 2;

[0084] (3) Add ambruticin, bakuchiol, and tocopheryl acetate to Mixture 2, heat to 65 °C, and mix and shear at 12,000 rpm for 4 min to prepare a uniform primary emulsion;

[0085] (4) Perform a high-pressure homogenization treatment on the primary emulsion in step (3) at 10,000 psi once, and then perform two microfluidic homogenization treatments at a pressure of 20,000 psi to obtain the anti-wrinkle and anti-aging composition containing ambruticin.

[0086] Example 4

[0087] An anti-wrinkle and anti-aging composition containing ambruticin, with the components by weight being: 2 parts of water-soluble active ingredients, 11 parts of oil-soluble active ingredients, and 87 parts of nano-encapsulated materials;

[0088] Among them, the water-soluble active ingredients are: 1 part of L-theanine and 1 part of VC ethyl ether;

[0089] The oil-soluble active ingredients are: 7 parts of ambruticin, 3 parts of Rosa canina fruit oil, and 1 part of tocopheryl acetate;

[0090] The nano-encapsulated materials are: 47 parts of butylene glycol, 21 parts of water, 14 parts of triglyceride caprylate / caprate, and 5 parts of hydrogenated lecithin.

[0091] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0092] (1) Mix butylene glycol, water, triglyceride caprylate / caprate, and hydrogenated lecithin, add them to a homogenization reactor, heat to 70 °C, and shear and stir evenly at 12,000 rpm to obtain Mixture 1;

[0093] (2) Add L-theanine and VC ethyl ether to Mixture 1, heat to 70 °C, and mix and stir evenly at 300 rpm to obtain Mixture 2;

[0094] (3) Add ambruticin, Rosa canina fruit oil, and tocopheryl acetate to Mixture 2, heat to 70 °C, and mix and shear at 16,000 rpm for 5 min to prepare a uniform primary emulsion;

[0095] (4) The colostrum from step (3) is subjected to a high-pressure homogenization treatment at 11,000 psi once, and then subjected to two microfluidic homogenization treatments at a pressure of 20,000 psi, thus obtaining the anti-wrinkle and anti-aging composition containing centaureamide.

[0096] Comparative Example 1

[0097] Different from Example 1, the theanine is replaced with aloe extract.

[0098] The anti-wrinkle and anti-aging composition, based on parts by weight, consists of: 1 part of water-soluble active ingredient, 10 parts of oil-soluble active ingredient, and 89 parts of nano-encapsulated material;

[0099] Among them, the water-soluble active ingredient is: 0.5 part of carnosine and 0.5 part of aloe extract;

[0100] The oil-soluble active ingredient is: 6 parts of centaureamide, 3 parts of tocopheryl acetate, and 1 part of phytol;

[0101] The nano-encapsulated material is: 49 parts of glycerol, 20 parts of water, 15 parts of caprylic / capric triglyceride, and 5 parts of lecithin.

[0102] The preparation method is as follows:

[0103] (1) Mix glycerol, water, caprylic / capric triglyceride, and lecithin, add them to a homogenization reactor, heat to 60 °C, and shear and stir evenly at 11,000 rpm to obtain Mixture 1;

[0104] (2) Add carnosine and aloe extract to Mixture 1, heat to 60 °C, and mix and stir evenly at 200 rpm to obtain Mixture 2;

[0105] (3) Add centaureamide, tocopheryl acetate, and phytol to Mixture 2, heat to 60 °C, and mix and shear at 15,000 rpm for 3 min to prepare a uniform colostrum;

[0106] (4) The colostrum from step (3) is subjected to a high-pressure homogenization treatment at 10,000 psi once, and then subjected to two microfluidic homogenization treatments at a pressure of 25,000 psi, thus obtaining the anti-wrinkle and anti-aging composition.

[0107] Comparative Example 2

[0108] Different from Example 1, the oil-soluble active ingredient is: 10 parts of centaureamide; the oil-soluble active ingredient has no tocopheryl acetate and phytol components.

[0109] The anti-wrinkle and anti-aging composition, based on parts by weight, consists of: 1 part of water-soluble active ingredient, 10 parts of oil-soluble active ingredient, and 89 parts of nano-encapsulated material;

[0110] Among them, the water-soluble active ingredients are: 0.5 parts of carnosine and 0.5 parts of theanine;

[0111] The oil-soluble active ingredient is: 10 parts of ambruticin amide;

[0112] The nano-encapsulating material is: 49 parts of glycerol, 20 parts of water, 15 parts of caprylic / capric triglyceride and 5 parts of lecithin.

[0113] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0114] (1) Mix glycerol, water, caprylic / capric triglyceride and lecithin, add them into a homogenizing reactor, heat to 60 °C, and shear and stir evenly at 11000 rpm to obtain mixture 1;

[0115] (2) Add carnosine and theanine to mixture 1, heat to 60 °C, and mix and stir evenly at 200 rpm to obtain mixture 2;

[0116] (3) Add ambruticin amide to mixture 2, heat to 55 °C, and mix and shear at 12000 rpm for 3 min to prepare a uniform primary emulsion;

[0117] (4) Perform a high-pressure homogenization treatment on the primary emulsion in step (3) at 10000 psi once, and then perform a microfluidic homogenization treatment at a pressure of 25000 psi twice to obtain the anti-wrinkle and anti-aging composition.

[0118] Comparative Example 3

[0119] The difference from Example 1 is that the oil-soluble active ingredient is 7.5 parts of tocopheryl acetate and 2.5 parts of phytol; there is no ambruticin amide in the oil-soluble active ingredient.

[0120] The anti-wrinkle and anti-aging composition, calculated by weight, contains: 1 part of water-soluble active ingredient (0.5 parts of carnosine and 0.5 parts of theanine), 10 parts of oil-soluble active ingredient and 89 parts of nano-encapsulating material (49 parts of glycerol, 20 parts of water, 15 parts of caprylic / capric triglyceride and 5 parts of lecithin);

[0121] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0122] (1) Mix glycerol, water, caprylic / capric triglyceride and lecithin, add them into a homogenizing reactor, heat to 60 °C, and shear and stir evenly at 11000 rpm to obtain mixture 1;

[0123] (2) Add carnosine and theanine to mixture 1, heat to 60 °C, and mix and stir evenly at 200 rpm to obtain mixture 2;

[0124] (3) Add tocopheryl acetate and phytol to the mixture 2, heat to 60 °C, and mix and shear at 15000 rpm for 3 min to prepare a uniform primary emulsion;

[0125] (4) Perform a high-pressure homogenization treatment on the primary emulsion in step (3) at 10000 psi once, and then perform a microfluidic homogenization treatment at a pressure of 25000 psi twice to obtain the anti-wrinkle and anti-aging composition.

[0126] Comparative Example 4

[0127] Different from Example 1, the components in the nano-encapsulated material are: 49 parts of glycerol, 20 parts of water, 15 parts of isopropyl palmitate, and 5 parts of lecithin.

[0128] The anti-wrinkle and anti-aging composition, based on parts by weight, consists of: 1 part of water-soluble active ingredient (0.5 part of carnosine and 0.5 part of L-theanine), 10 parts of oil-soluble active ingredient, and 89 parts of nano-encapsulated material (49 parts of glycerol, 20 parts of water, 15 parts of isopropyl palmitate, and 5 parts of lecithin).

[0129] The preparation method of the anti-wrinkle and anti-aging composition is as follows:

[0130] (1) Mix glycerol, water, isopropyl palmitate, and lecithin, add them to a homogenization reactor, heat to 60 °C, and shear and stir evenly at 11000 rpm to obtain mixture 1;

[0131] (2) Add carnosine and L-theanine to mixture 1, heat to 60 °C, and mix and stir evenly at 200 rpm to obtain mixture 2;

[0132] (3) Add ambruticin amide, tocopheryl acetate, and phytol to mixture 2, heat to 60 °C, and mix and shear at 15000 rpm for 3 min to prepare a uniform primary emulsion;

[0133] (4) Perform a high-pressure homogenization treatment on the primary emulsion in step (3) at 10000 psi once, and then perform a microfluidic homogenization treatment at a pressure of 25000 psi twice to obtain the anti-wrinkle and anti-aging composition.

[0134] Comparative Example 5

[0135] Different from Example 1, the component ratios in the anti-wrinkle and anti-aging composition are different: 10 parts of water-soluble active ingredient, 3 parts of oil-soluble active ingredient, and 87 parts of nano-encapsulated material.

[0136] Among them, the water-soluble active ingredient is: 5 parts of carnosine and 5 parts of L-theanine;

[0137] The oil-soluble active ingredient is: 1.8 parts of ambruticin amide, 0.9 part of tocopheryl acetate, and 0.3 part of phytol;

[0138] The nano-encapsulated materials are glycerol, water, triglyceride caprylate / caprate, and lecithin. The mass ratio of glycerol, water, triglyceride caprylate / caprate, and lecithin is 49:20:15:5. 87 parts of the nano-encapsulated materials are taken to prepare the anti-wrinkle and anti-aging composition of this comparative example.

[0139] The preparation method of the anti-wrinkle and anti-aging composition is the same as that of Example 1.

[0140] Test 1 Detection of collagen promotion effect

[0141] Experimental method

[0142] (1) Cell seeding: Seed cells into a 24-well plate at an inoculation density of (8×10 4 / well), and incubate overnight in an incubator (37°C, 5% CO2); the cells are fibroblasts, batch number: Fb19052002, provided by Weiyue (Jiangmen) Biotechnology Co., Ltd.

[0143] (2) Experimental grouping: blank control group, negative control group, positive control group, and sample groups (Example groups and comparative example groups). Three concentration gradients (1%, 2.5%, 5%) are set for the sample group of Example 1; the remaining Example and comparative example groups are set at a volume concentration of 5%.

[0144] (3) Solution preparation: Using cell culture medium (DMEM medium with 10% fetal bovine serum), prepare the composition working solutions with different volume concentrations (v / v) of the anti-wrinkle and anti-aging composition: low dose 1%, medium dose 2.5%, high dose 5%.

[0145] (4) Drug administration: When the cell confluence rate in the 24-well plate reaches 40%-60%, drug administration is carried out. 1 mL of cell culture medium is added to each well of the blank control group and the negative control group; 1 mL of the culture medium containing 100 μg / mL vitamin C and 7 μg / mL vitamin E is added to each well of the positive control group; 1 mL of the working solution containing the anti-wrinkle and anti-aging composition with the corresponding concentration is added to each well of the sample group.

[0146] (5) Radiation: 24 hours after drug administration, the negative control group, positive control group, and sample groups are respectively irradiated with UVA radiation with a total dose of 9 J / cm 2 ; meanwhile, the blank control group is placed in the same environment (UVA radiation dose is 0 J / cm 2 ).

[0147] (6) Collect cell supernatant: After incubating and culturing for 24 hours, collect the cell culture supernatant into an EP tube and store it frozen in an -80°C refrigerator.

[0148] (7) Cell collection: After incubating for 24 h, add 0.5 mL of lysis buffer to each well for lysis; collect the cell lysate in an EP tube, and after collection, place the cell lysate sample for detection in a -80 °C refrigerator for freezing and storage.

[0149] (9) Collagen I detection: Detect and analyze the content of Collagen I according to the operation manual of the ELISA detection kit (enzyme-linked immunosorbent assay kit).

[0150] The detection results of the Collagen I content in each group are shown in Table 1.

[0151] Table 1

[0152]

[0153] In Table 1, compared with the NC group and the BC group, the significance is indicated by ## , p-value < 0.01 is indicated by ## ; compared with the NC group, the PC group and the sample group, the significance is indicated by & , && , p-value < 0.05 is indicated by & , p-value < 0.01 is indicated by && .

[0154] It can be seen from Table 1 that compared with the BC group, after the NC group of fibroblasts received UVA radiation with a total dose of 9 J / cm 2 , the content level of Collagen I decreased significantly (p < 0.01), indicating that the irradiation stimulation was effective.

[0155] Compared with the NC group, vitamin C and vitamin E in the PC group can significantly increase the content level of Collagen I (p < 0.01), indicating that the positive control detection in this experiment was effective.

[0156] Compared with the NC group, the samples in Example 1 showed a significant promoting effect on the Collagen I content of UVA-irradiated fibroblasts at volume concentrations of 1%, 2.5%, and 5% (p < 0.05, p < 0.01); the samples in Example 2 - Example 4 groups all showed a significant promoting effect on the Collagen I content of UVA-irradiated fibroblasts, indicating that the samples in the examples not only have the effect of resisting UVA light damage, but also have the effect of anti-wrinkle and firming.

[0157] Experiment 2 Anti-wrinkle efficacy test

[0158] The anti-wrinkle and anti-aging compositions of each example and comparative example were respectively used to prepare cosmetics. Take 5 g of the anti-wrinkle and anti-aging composition, 2 g of polyacrylate crosspolymer-6, 3 g of butanediol, 1 g of raspberry ketone and 89 g of water, and shear with a homogenizer at 6000 rpm for 6 min to prepare the anti-wrinkle and anti-aging cosmetics.

[0159] (1) Test purpose: To evaluate the anti-wrinkle efficacy of the anti-wrinkle and anti-aging composition samples by measuring the change in the wrinkle feature count of healthy subjects before / after using the test samples.

[0160] (2) Subjects: Healthy men and women who meet the test requirements and have not taken or topically used any drugs; aged 30 - 50 years old, with an equal number of men and women; the subjects can maintain regular life during the study; randomly divided into 10 groups, with 10 people in each group.

[0161] (3) Test method: According to the anti-aging efficacy evaluation method in the "Cosmetics Efficacy Claim Evaluation Specification", the objective instrument evaluation method was used for detection. Test parameters: Wrinkle feature count; Instrument: Skin image analyzer; Test time points: Day 0 (denoted as D0), Day 28 (denoted as D28); Test site: Face; Test method: Clean the face with a cleansing product and dry the skin with a dry paper towel. After sitting still in a laboratory at a temperature of 21 ± 1°C and 50 ± 10% RH for 30 min, use the skin image analyzer to collect and count the facial wrinkle features.

[0162] (4) Cosmetics usage site: Face; Usage frequency: Twice a day, once in the morning and once in the evening; Usage period: 4 weeks; Usage method: Apply all over the face; Usage amount: Approximately 2 g of cosmetics per application. The subjects in the blank group did not use cosmetics but used a basic moisturizing lotion for moisturizing; the subjects in the example group and the comparative example group used a basic moisturizing lotion for moisturizing and then used the corresponding cosmetics respectively.

[0163] Wrinkle feature decline rate (%) = (D0 wrinkle feature result - D28 wrinkle feature result) / D0 wrinkle feature result × 100%.

[0164] The technical statistical results of the wrinkle features are shown in Table 2.

[0165] Table 2

[0166] Example D0 (nm) D28 (nm) Wrinkling feature decline rate (%) Blank group 118.86±20.65 119.23±19.24 -0.3 Example 1 120.30±18.65 80.20±20.05 33.3 Example 2 119.31±18.55 78.89±20.01 33.9 Example 3 120.90±18.69 79.10±20.15 34.6 Example 4 121.32±18.15 79.57±18.05 34.4 Comparative example 1 120.99±18.61 101.21±20.11 16.3 Comparative example 2 121.01±18.69 103.80±20.02 14.2 Comparative example 3 122.09±17.25 106.16±16.27 13.0 Comparative example 4 121.10±18.29 105.20±29.75 13.1 Comparative example 5 121.41±18.20 108.97±21.70 10.2

[0167] As can be seen from Table 2, the cosmetics prepared from the anti-wrinkle and anti-aging composition of the embodiments of the present invention can significantly reduce the number and length of wrinkles, achieving a remarkable effect of wrinkle fading; compared with the cosmetics of the comparative examples, the samples of Examples 1-4 have better wrinkle fading effects, indicating that in the anti-wrinkle and anti-aging composition of the present invention, the components interact with each other and are combined with each other in a specific ratio, bringing remarkable effects of wrinkle removal and increasing collagen content.

[0168] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than limiting the protection scope of the present invention. Any simple modification or equivalent replacement made by those of ordinary skill in the art to the technical solution of the present invention shall not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A wrinkle-removing and anti-aging composition containing acileacin, characterized in that, By weight, it consists of the following components: 0.5 - 2 parts of water-soluble active ingredients, 10 - 14 parts of oil-soluble active ingredients, and 85 - 89 parts of nano-encapsulated materials; The water-soluble active ingredients are selected from at least 2 of carnosine, L-theanine, and VC ethyl ether; The oil-soluble active ingredients are selected from at least 3 of ambruticin amide, Rosa roxburghii tratt seed oil, bakuchiol, tocopheryl acetate, and phytol; The nano-encapsulated materials are selected from at least 4 of glycerol, 1,3-propanediol, butanediol, water, caprylic / capric triglyceride, lecithin, and hydrogenated lecithin.

2. The anti-wrinkle and anti-aging composition according to claim 1, characterized in that, The weight ratio of the water-soluble active ingredients, oil-soluble active ingredients, and nano-encapsulated materials is 1:10:

89.

3. The anti-wrinkle and anti-aging composition according to any one of claims 1-2, characterized in that The water-soluble active ingredient is a mixture of carnosine and L-theanine; or a mixture of carnosine and VC ethyl ether.

4. The anti-wrinkle and anti-aging composition according to any one of claims 1-2, characterized in that The oil-soluble active ingredient is a mixture of ambruticin amide, tocopheryl acetate, and a third oil-soluble active ingredient; the weight ratio of ambruticin amide, tocopheryl acetate, and the third oil-soluble active ingredient is 6 - 9:1 - 4:1 - 4.

5. The anti-wrinkle and anti-aging composition according to claim 4, wherein The third oil-soluble active ingredient is selected from one of phytol, bakuchiol, and Rosa roxburghii tratt seed oil.

6. The anti-wrinkle and anti-aging composition according to claim 1, characterized in that, The nano-encapsulated material is a mixture of a first nano-encapsulated material, a second nano-encapsulated material, a third nano-encapsulated material, and a fourth nano-encapsulated material; the first nano-encapsulated material is selected from at least one of glycerol, 1,3-propanediol, and butanediol; the second nano-encapsulated material is water; the third nano-encapsulated material is caprylic / capric triglyceride; the fourth nano-encapsulated material is lecithin or hydrogenated lecithin.

7. The anti-wrinkle and anti-aging composition according to claim 6, characterized in that, In the nano-encapsulated material, the mass ratio of the first nano-encapsulated material, the second nano-encapsulated material, the third nano-encapsulated material, and the fourth nano-encapsulated material is 40 - 49:15 - 22.5:14 - 20:

5.

8. The preparation method of the anti-wrinkle and anti-aging composition according to any one of claims 1-7, characterized in that, It includes the steps: (1) Mix the components of the nano-encapsulated material and shear to obtain mixture 1; (2) Mix mixture 1 from step (1) with the water-soluble active ingredients to obtain mixture 2; (3) Mix mixture 2 from step (2) with the oil-soluble active ingredients and shear to obtain the anti-wrinkle and anti-aging composition.

9. The application of the anti-wrinkle and anti-aging composition according to any one of claims 1 - 7 or the anti-wrinkle and anti-aging composition prepared by the preparation method according to claim 8 in cosmetics.

10. A cosmetic with wrinkle-removing and anti-aging effects, characterized in that, It includes the component: the anti-wrinkle and anti-aging composition according to any one of claims 1 - 7 or the anti-wrinkle and anti-aging composition prepared by the preparation method according to claim 8.

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