Anti-aging moisturizing essence and preparation method thereof

Abstract

The invention provides anti-aging moisturizing essence and a preparation method thereof, and belongs to the technical field of cosmetics. The essence is prepared from a plant extract, sodium pyrrolidone carboxylate, hexapeptide, nicotinamide mononucleotide, vitamin B5, galactose yeast fermentation product filtrate, b-glucan, sodium hyaluronate, glycerol, tranexamic acid, blue copper peptide, arbutin, nicotinamide, vitrin, a bifidus yeast fermentation product, a bifidus yeast fermentation lysate and the like. Wherein the plant extract is prepared by mixing rosemary, frankincense resin, tea flowers, sandalwood and jasmine flowers through ultrasonic extraction, comprehensive skin care is achieved from multiple angles of anti-aging, whitening, moisturizing and repairing, the overall health of the skin is guaranteed, and the skin care cream is suitable for various skin types.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to an anti-aging and moisturizing essence and a preparation method thereof. Background Art

[0002] Skin aging is a complex physiological process, typically manifested by decreased skin elasticity, increased wrinkles, dryness, and sagging. Modern scientific research indicates that skin aging is not only related to natural aging but also influenced by a variety of external factors, including environmental factors, lifestyle, and UV exposure. Therefore, the development of skincare products that effectively delay aging and provide moisturizing and nourishing effects has become a key market demand.

[0003] Currently, there are a wide variety of anti-aging moisturizing serums on the market, but some common problems exist. On the one hand, some products pursue immediate results and add large amounts of film-forming agents, fillers, and other ingredients. Although these can improve the skin's appearance in the short term, they are unlikely to fundamentally address the problem of aging. On the other hand, in pursuit of strong anti-aging effects, high concentrations of active ingredients or irritating raw materials are often used, which may cause adverse reactions such as redness, swelling, and peeling on the skin, affecting the user experience. At the same time, the active ingredients in existing anti-aging moisturizing serums often have a large molecular weight, making it difficult for them to effectively penetrate deep into the skin, thus limiting their effectiveness. For example, large molecules such as collagen and hyaluronic acid have difficulty penetrating the skin barrier and can only work on the skin's surface, failing to fundamentally improve skin aging.

[0004] Based on this, it is urgent to provide an anti-aging moisturizing essence. Summary of the Invention

[0005] In view of the defects of the prior art, the present invention designs an anti-aging moisturizing essence.

[0006] In order to achieve the above purpose, the present invention solves the technical problem by adopting the following technical solutions:

[0007] In a first aspect, the present invention provides an anti-aging moisturizing essence, comprising, by mass percentage, 18-23% plant extract, 0.5-3% sodium p-hydroxybenzoate, 0.5-3% hexapeptide, 1-5% nicotinamide mononucleotide, 0.5-3% vitamin B5, 2-8% galactosidase fermentation product filtrate, 0.1-2% b-glucan, 5-10% sodium hyaluronate, 10-20% glycerol, 0.5-3% tranexamic acid, 0.4-0.8% blue copper peptide, 0.5-1.5% arbutin, 1-3% niacinamide, 0.8-1.2% bosine, 1-3% bifid yeast fermentation product, 1-3% bifid yeast fermentation lysate, and the balance water;

[0008] The plant extract is prepared by mixing rosemary leaves, frankincense resin, tea tree flowers, sandalwood and jasmine flowers in a mass ratio of (3-8): (3-9): (1-3): (3-8): (1-3) and then subjecting the mixture to ultrasonic extraction.

[0009] Among them, the hexapeptide can promote collagen production and enhance skin firmness and elasticity; NMN enhances cell energy metabolism and helps restore skin vitality; blue copper peptide promotes wound healing, improves skin texture, and enhances skin repair ability; and niacinamide improves uneven skin tone, enhances skin barrier function, and provides antioxidant protection.

[0010] In terms of brightening effects, the combined use of tranexamic acid and arbutin effectively inhibits melanin production, enhancing skin brightness. Meanwhile, the combination of sodium hyaluronate and glycerin deeply moisturizes the skin and locks in moisture, while galactosyl ferment filtrate and beta-glucan enhance the skin's repair capacity and promote overall skin health.

[0011] In some preferred embodiments, the composition comprises, by mass percentage, 20% plant extract, 2% sodium hydroxybenzoate, 2% hexapeptide, 3% nicotinamide mononucleotide, 2% vitamin B5, 5% galactosidase fermentation product filtrate, 1% b-glucan, 7% sodium hyaluronate, 15% glycerol, 2% tranexamic acid, 0.6% blue copper peptide, 1% arbutin, 2% nicotinamide, 1% bosine, 2% bifid yeast fermentation product, 2% bifid yeast fermentation lysate, and the balance water;

[0012] The plant extract is prepared by ultrasonic extraction after mixing rosemary leaves, frankincense resin, tea tree flowers, sandalwood and jasmine flowers in a mass ratio of 5:6:2:5:2.

[0013] In some preferred embodiments, the anti-aging moisturizing essence further comprises 5-6% of seaweed fermentation product.

[0014] In some preferred embodiments, the preparation of the seaweed fermentation product comprises:

[0015] S1. Grind chlorella, spirulina, and antlers through an 80-mesh sieve;

[0016] S2. Mix 2-8 parts of Chlorella, 2-8 parts of Spirulina, 5-10 parts of Antlers of Sea, and 5-15 parts of sucrose by weight, sterilize the mixture, inoculate with Kluyveromyces marxianus for fermentation, and then sterilize and filter to obtain a seaweed fermentation broth.

[0017] In some preferred embodiments, the inoculation amount of Kluyveromyces marxianus is 3-7 wt %.

[0018] In some preferred embodiments, the viable cell count of Kluyveromyces marxianus is 1×10 8CFU / mL.

[0019] In some preferred embodiments, the fermentation temperature is 25-30° C., and the fermentation time is 75-85 h.

[0020] In a second aspect, the present invention provides a method for preparing the above-mentioned anti-aging moisturizing essence, the preparation method comprising:

[0021] Add plant extract, sodium pyridonecarboxylate, hexapeptide, nicotinamide mononucleotide, vitamin B5, galactosidase fermentation product filtrate, b-glucan, sodium hyaluronate, glycerin, tranexamic acid, blue copper peptide, arbutin, niacinamide, bosine, bifid yeast fermentation product, bifid yeast fermentation lysate, and water into a stirring pot and stir at 40-60 rpm for 20-40 minutes to obtain an anti-aging moisturizing essence.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The present invention adopts a triple compound anti-aging mechanism, namely the neurotransmitter inhibition layer of hexapeptide and blue copper peptide, the cellular energy metabolism layer of nicotinamide mononucleotide and galactosidase ferment, and the matrix reconstruction layer of bosera and bifid yeast ferment, and synergistically enhances the effect with plant extracts. The prepared essence not only has good antioxidant, anti-inflammatory and soothing effects, but also ensures significant effects in anti-aging and cell repair, can effectively improve skin condition, avoid the problems of insufficient moisturizing durability and easy water loss, ensure the effectiveness and stability of active ingredients, and is beneficial to maintaining skin barrier function and skin cell metabolism, thereby achieving anti-aging-whitening-moisturizing-repair multi-angle comprehensive skin care, and ensuring the overall health of the skin.

[0024] At the same time, the essence of the present invention also contains seaweed fermentation products, which convert seaweed macromolecules into small molecule active substances through a staged fermentation process, making it easier to penetrate into the dermis, thereby improving the absorption efficiency of ingredients such as hexapeptides, further inhibiting the release of neurotransmitters, reducing dynamic wrinkles, enhancing the inhibitory effect of elastase, and protecting the integrity of collagen structure. It also cooperates with ingredients such as bifid yeast fermentation products and galactose yeast fermentation product filtrate to promote DNA repair, enhance skin elasticity, and reduce the decline in cell function caused by DNA damage, thereby delaying skin aging and reducing the formation of wrinkles. In addition, seaweed fermentation products are rich in minerals and amino acids, forming a water-locking film, and combined with hyaluronic acid to further enhance the skin's water retention. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solution of the present invention, the following is a brief introduction to the drawings required for use in the implementation. Obviously, the drawings described below are only some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0026] Figure 1 This is a test result chart of the moisturizing rate of different essences.

[0027] Figure 2 This is the test result of the inhibition rate of different essences on elastase. DETAILED DESCRIPTION

[0028] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0029] In the present invention, when referring to numerical ranges, unless otherwise specified, the numerical ranges are considered continuous and include the minimum and maximum values ​​of the range, as well as every value between such minimum and maximum values. Furthermore, when a range refers to an integer, every integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe a feature or characteristic, the ranges can be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges subsumed therein.

[0030] In the present invention, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0031] In the present invention, the test methods used are conventional methods unless otherwise specified; the materials, reagents, etc. used are reagents and materials that can be obtained from commercial channels unless otherwise specified.

[0032] In the present invention, the INCI name of the "galactosidase fermentation product filtrate" is GALACTOMYCESFERMENT FILTRATE.

[0033] In the present invention, the INCI name of the "Bifid Saccharomyces cerevisiae fermentation product" is BIFIDA FERMENT EXTRACT.

[0034] In the present invention, the INCI name of the "Bifid yeast fermentation lysate" is BIFIDA FERMENT LYSATE, CAS: No. 96507-89-0.

[0035] In the present invention, the preparation of the "Kluyveromyces marxianus seed solution" is:

[0036] The frozen Kluyveromyces marxianus (strain number: CICC 1953, purchased from China Industrial Microbiological Culture Collection) was activated and cultured, then inoculated on MEA medium and cultured at 28°C until the viable count reached 1×10 8 cfu / mL, and obtain the Kluyveromyces marxianus seed liquid.

[0037] Example 1

[0038] 1. An anti-aging moisturizing essence, comprising, by mass percentage, 20% plant extract, 2% sodium hydroxybenzoate, 2% hexapeptide, 3% nicotinamide mononucleotide, 2% vitamin B5, 5% galactosidase fermentation product filtrate, 1% b-glucan, 7% sodium hyaluronate, 15% glycerin, 2% tranexamic acid, 0.6% blue copper peptide, 1% arbutin, 2% niacinamide, 1% bosine, 2% bifid yeast fermentation product, 2% bifid yeast fermentation lysate, and the balance purified water;

[0039] Wherein, the preparation of the plant extract comprises the following steps:

[0040] (1) Wash rosemary leaves, frankincense resin, tea tree flowers, sandalwood, and jasmine flowers separately, dry them at 50° C. to a moisture content of ≤10%, and grind them through an 80-mesh sieve.

[0041] (2) Rosemary leaves, frankincense resin, tea tree flowers, sandalwood, and jasmine powder were mixed in a mass ratio of 5:6:2:5:2, and then added with 80% ethanol solution at a material-liquid ratio of 1:20 (g / mL). Ultrasonic extraction was performed twice at 50°C (power 300 W, frequency 40 kHz, each time for 1 h).

[0042] (3) The two extracts were combined, concentrated under reduced pressure at 50°C to a relative density of 1.3 (measured at 25°C), and freeze-dried at -50°C for 24 hours to obtain the plant extract.

[0043] 2. A method for preparing an anti-aging moisturizing essence, comprising the following steps:

[0044] Add plant extract, sodium pyrrolidonecarboxylate, hexapeptide, nicotinamide mononucleotide, vitamin B5, galactosidase fermentation product filtrate, b-glucan, sodium hyaluronate, glycerin, tranexamic acid, blue copper peptide, arbutin, niacinamide, bosine, bifid yeast fermentation product, bifid yeast fermentation lysate and purified water into a stirring pot and stir at 80 rpm for 30 minutes to obtain an anti-aging moisturizing essence.

[0045] Example 2

[0046] 1. An anti-aging moisturizing essence, comprising, by mass percentage, 18% plant extract, 3% sodium p-hydroxybenzoate, 0.5% hexapeptide, 5% NMN (nicotinamide mononucleotide), 3% vitamin B5, 2% galactosidase fermentation product filtrate, 2% b-glucan, 5% sodium hyaluronate, 20% glycerin, 3% tranexamic acid, 0.4% blue copper peptide, 0.5% arbutin, 1% niacinamide, 1.2% bosine, 3% bifid yeast fermentation product, 1% bifid yeast fermentation lysate, and the balance water.

[0047] Wherein, the preparation of the plant extract comprises the following steps:

[0048] (1) Wash rosemary leaves, frankincense resin, tea tree flowers, sandalwood, and jasmine flowers separately, dry them at 50° C. to a moisture content of ≤10%, and grind them through an 80-mesh sieve.

[0049] (2) Rosemary leaves, frankincense resin, tea tree flowers, sandalwood, and jasmine powder were mixed in a mass ratio of 8:3:1:3:3, and then added with 80% ethanol solution at a material-liquid ratio of 1:20 (g / mL). Ultrasonic extraction was performed twice at 50°C (power 300 W, frequency 40 kHz, each time for 1 h).

[0050] (3) The two extracts were combined, concentrated under reduced pressure at 50°C to 1 / 5 of their mass, and freeze-dried at -50°C for 24 h to obtain the plant extract.

[0051] 2. The preparation method of an anti-aging moisturizing essence is the same as that of Example 1.

[0052] Example 3

[0053] 1. An anti-aging moisturizing essence, comprising, by mass percentage, 23% plant extract, 0.5% sodium p-hydroxybenzoate, 3% hexapeptide, 1% NMN (nicotinamide mononucleotide), 0.5% vitamin B5, 8% galactosidase fermentation product filtrate, 0.1% b-glucan, 10% sodium hyaluronate, 10% glycerol, 0.5% tranexamic acid, 0.8% blue copper peptide, 1.5% arbutin, 3% niacinamide, 0.8% bosine, 1% bifid yeast fermentation product, 3% bifid yeast fermentation lysate, and the balance water.

[0054] Wherein, the preparation of the plant extract comprises the following steps:

[0055] (1) Wash rosemary leaves, frankincense resin, tea tree flowers, sandalwood, and jasmine flowers separately, dry them at 50° C. to a moisture content of ≤10%, and grind them through an 80-mesh sieve.

[0056] (2) Rosemary leaves, frankincense resin, tea tree flowers, sandalwood, and jasmine powder were mixed in a mass ratio of 3:8:3:8:1, and then added with 80% ethanol solution at a material-liquid ratio of 1:20 (g / mL). Ultrasonic extraction was performed twice at 50°C (power 300 W, frequency 40 kHz, each time for 1 h).

[0057] (3) The two extracts were combined, concentrated under reduced pressure at 50°C to 1 / 5 of their mass, and freeze-dried at -50°C for 24 h to obtain the plant extract.

[0058] 2. The preparation method of an anti-aging moisturizing essence is the same as that of Example 1.

[0059] Example 4

[0060] The difference between Example 4 and Example 1 is that Example 4 further includes 5% seaweed fermentation product, and all other aspects are the same.

[0061] The preparation of the seaweed fermentation product comprises:

[0062] (1) Chlorella, Spirulina, and Antleria salsa were washed separately, dried at 50°C until the moisture content was ≤10%, and crushed through an 80-mesh sieve;

[0063] (2) According to weight, 5 parts of Chlorella, 5 parts of Spirulina, 8 parts of Antlers horns, and 10 parts of sucrose were mixed and sterilized, and then inoculated with Kluyveromyces marxianus and fermented at 28°C for 80 hours. The mixture was then sterilized and filtered to obtain the seaweed fermentation liquid.

[0064] The inoculation amount of the Kluyveromyces marxianus was 5 wt %.

[0065] During the fermentation process, two stages of fermentation are adopted, namely:

[0066] Phase 1 (0-30h): maintain pH 5.5-6.0, dissolved oxygen ≤10%;

[0067] The second stage (30-80h): adjust the pH to 4.0-4.5 and the dissolved oxygen content ≤5%.

[0068] Example 5

[0069] The difference between Example 5 and Example 4 is that the amount of seaweed fermentation product added in Example 5 is 6%, and the other aspects are the same.

[0070] Comparative Example 1

[0071] The difference between Comparative Example 1 and Example 1 is that no plant extract is added in Comparative Example 1, and all other aspects are the same.

[0072] Comparative Example 2

[0073] Comparative Example 2 differs from Example 1 in that the plant extracts are different, and all other aspects are the same.

[0074] The plant extract is prepared by ultrasonic extraction after mixing green tea leaves, aloe leaves, tea tree flowers, liquorice and jasmine flowers in a mass ratio of 9:12:5:5:7.

[0075] Comparative Example 3

[0076] Comparative Example 3 differs from Example 1 in that an equal amount of tripeptide-1 is used instead of hexapeptide, and all other conditions are the same.

[0077] Test Example 1

[0078] Free radicals are one of the main causes of oxidative stress during aging, damaging cell membranes, proteins, and DNA. Therefore, the anti-aging potential of serums was measured using a superoxide anion radical scavenging rate assay (pyrogallol autooxidation method) and a hydroxyl radical scavenging rate assay (Fenton oxidation method). The scavenging rate was calculated using the following formula:

[0079] Calculation formula for superoxide anion free radical scavenging rate (%):

[0080]

[0081] Calculation formula for hydroxyl radical scavenging rate (%):

[0082]

[0083] Table 1. Scavenging rate of free radicals by different essences

[0084]

[0085] As can be seen from Table 1, compared with Comparative Examples 1-2, Examples 1-3 have higher free radical scavenging rates, indicating that the essence of the present invention has stronger antioxidant capacity; then, Comparative Examples 4-5 contain seaweed fermentation products, and their free radical scavenging rates are higher than those of Examples 1-3 without seaweed fermentation products. This is because seaweed fermentation products can neutralize free radicals and convert seaweed macromolecules into small molecules through a staged fermentation process, which are easier to penetrate into the dermis, thereby improving the absorption efficiency of ingredients such as hexapeptide and blue copper peptide, further enhancing antioxidant capacity, and further delaying skin aging.

[0086] Test Example 2

[0087] 1. Analyze the in vitro moisturizing rate of different essences.

[0088] Test Principle: A supersaturated ammonium sulfate solution is placed in a desiccator to control humidity. A portion of medical 3M tape simulates the skin surface environment. Liquid is dispersed on the tape, placed in a constant humidity environment, and weighed periodically to calculate the moisture retention, or moisturizing rate.

[0089] Test sample:

[0090] Control group: purified water; Experimental group: essences prepared in Example 1, Example 4, and Comparative Examples 1-2.

[0091] Test steps: Add saturated ammonium sulfate solution to the bottom of a desiccator to achieve constant humidity. Take a 5cm x 5cm glass plate and tape it with 3M tape. Use a porous partition to separate the saturated ammonium sulfate solution. Place the glass plate in the desiccator and let it sit for 20 minutes. Weigh the glass plate (including the 3M tape) to the nearest 0.0001g. Repeat three times for each test sample.

[0092] Apply the test sample evenly on the 3M tape on the glass plate. The sample dosage is 2 mg / cm 2 , weigh as m0, accurate to 0.0001g, put it into constant humidity desiccant immediately after weighing and let it stand for 1h, 2h, 4h, 8h, 24h, 48h respectively, and calculate the moisture retention rate at each time point.

[0093] Result analysis:

[0094] Depend on Figure 1 As can be seen, each test sample exhibited the best moisturizing effect at 1 hour, with the moisturizing rate decreasing over time. However, the moisturizing rate of Example 1 reached a high of 30.38% at 1 hour, and continued to reach 16.05% at 48 hours, significantly superior to that of Comparative Examples 1-2 and water. This demonstrates that the essence of the present invention has a superior moisturizing rate. The essences prepared in Examples 2 and 3 were the same as those in Example 1 and are not described again here.

[0095] Furthermore, by comparing Example 4 with Example 1, it can be seen that the moisturizing rate of Example 4 is significantly higher than that of Example 1. This is because the seaweed fermentation product and ingredients such as hyaluronic acid synergistically lock in moisture, increase the skin's moisture content, and at the same time stimulate the expression of tight junction proteins to reduce transepidermal water loss.

[0096] 2. Analysis of the effects of different essences on elastase

[0097] Elastase has the ability to degrade various proteins, including collagen and elastin. Changes in elastin structure during skin aging are a key cause of wrinkles and sagging. Therefore, we analyze the anti-wrinkle efficacy of serums by testing whether they inhibit elastase.

[0098] Test samples: the essences prepared in Example 1, Example 4, and Comparative Examples 1-3.

[0099] Preprocessing:

[0100] Prepare essence with concentrations of 10%, 30%, 50%, 60%, and 80%, then add orcein elastin and borate buffer solution, shake in a constant temperature of 37°C water bath for 10 minutes, then add 1 mL of elastase solution, measure the absorbance at 495 nm, and calculate the elastase inhibition rate.

[0101] Test method: MC-ZY-162 elastase inhibition rate method (from China Science Testing Technology Service (Guangzhou) Co., Ltd.)

[0102] Result analysis:

[0103] Depend on Figure 2 As can be seen, all the essences have a significant inhibitory effect on elastase, and the inhibition rate of elastase increases linearly with increasing sample concentration. In particular, the essence prepared in Example 1 still has an elastase inhibition rate of 31.74% at a concentration of 10%, and at the highest concentration, it still has an inhibition rate of 75.65%, which is significantly better than Comparative Examples 1-3 and water, indicating that the essence of the present invention has significant anti-wrinkle efficacy. In addition, compared with Comparative Example 3, which added tripeptide-1, the hexapeptide added in Example 1 not only moisturizes but also enhances the anti-wrinkle effect of the essence.

[0104] Then, compared with Example 1 without adding seaweed fermentation product, Example 4 adding seaweed fermentation product significantly improved the elastase inhibition rate, indicating that seaweed fermentation product can enhance the elastase inhibition effect, thereby reducing the decomposition of elastin, which is beneficial to delaying skin aging.

[0105] Test Example 3

[0106] Test the effects of different essences on fibroblast viability (MTT method)

[0107] Test sample:

[0108] Control group: not irradiated with ultraviolet light; Model group: irradiated with ultraviolet light;

[0109] 100 μL of 1×10 4 / mL human skin fibroblast ESF-1 suspension was inoculated into a 96-well plate and cultured at 37°C and 5% CO2. The culture medium was changed every other day. When the cell fusion reached more than 80%, the essence prepared in Example 1 and Comparative Example 1-2 (concentration of 50 mg / L) was added respectively and incubated for 2 hours. The cells were then exposed to ultraviolet light (20 J / cm 2) After irradiation, cells were incubated for another 24 hours. 5 mg / mL MTT reagent was then added and incubated for 4 hours before terminating the culture. The supernatant was discarded, and 150 μL of 2% DMSO was added to each well. The cells were shaken and mixed at room temperature for 15 minutes. The absorbance (A) of the cells was measured at 490 nm using a microplate reader, and cell viability was calculated. The calculation formula is:

[0110]

[0111] Where A is the absorbance value of the experimental group cells; A0 is the absorbance value of the control group cells.

[0112] Table 2 Cell viability test results

[0113] Group Cell viability (%) control group 99.15 Model Group 36.92 Example 1 75.89 Example 4 82.56 Comparative Example 1 48.47 Comparative Example 2 69.76

[0114] As shown in Table 2, compared with the control group, the cell viability of the model group decreased significantly, and after using the essence, the cell viability increased significantly. In particular, after using the essence of Example 4, the viability of human skin fibroblasts was as high as 82.56%. This shows that the essence of the present invention helps to enhance the skin's repair ability, maintain the skin's barrier function, and keep the skin healthy.

[0115] Test Example 4

[0116] 80 volunteers aged 30-55 were randomly divided into 4 groups, with 20 participants in each group. After cleansing their faces in the morning and evening, the volunteers were smeared with 2 mL of the essences of Examples 1 and 4 and Comparative Examples 1 and 2, respectively. The skin conditions were tested before and after 12 weeks of application.

[0117] Test indicators:

[0118] Skin whiteness: The skin whiteness of one random cheek was measured using a Skin-Colorimeter CL400.

[0119] Skin glossiness: Use the Skin-Glossymeter GL200 to measure the glossiness of the skin on a random side of the cheek. (The larger the measured value, the better the skin glossiness)

[0120] Table 3 Skin test results of using different essences for 12 weeks

[0121]

[0122] As shown in Table 3, after using the essences of Examples 1 and 4 for 12 weeks, the skin whiteness and glossiness are much higher than those of Comparative Examples 1 and 2, indicating that the essences of the present invention have a good whitening effect.

[0123] In summary, the present invention prepares an essence by combining plant extracts prepared from rosemary, frankincense resin, tea tree flower, sandalwood, and jasmine, sodium p-hydroxybutyrate, hexapeptide, nicotinamide mononucleotide, vitamin B5, galactosidase fermentation product filtrate, b-glucan, sodium hyaluronate, glycerol, tranexamic acid, blue copper peptide, arbutin, nicotinamide, bosine, bifid yeast fermentation product, 1-3% bifid yeast fermentation lysate, etc., which has good anti-aging and moisturizing effects.

[0124] On this basis, the essence of the present invention also includes seaweed fermentation products obtained by staged fermentation of seaweed, which can further enhance the anti-aging and moisturizing effects and effectively penetrate deep into the skin. Compared with other plant compositions, it can exert greater efficacy. However, if the essence of the present invention lacks or replaces a certain component, the balance of the system will be disrupted, thereby affecting the anti-aging and moisturizing effects. Therefore, the various components of the essence of the present invention cooperate and synergize with each other within a certain range, while ensuring the safety of the product while achieving anti-aging and moisturizing effects.

[0125] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. An anti-aging moisturizing essence, characterized in that: By mass percentage, it includes 18-23% plant extract, 0.5-3% sodium p-hydroxybenzoate, 0.5-3% hexapeptide, 1-5% nicotinamide mononucleotide, 0.5-3% vitamin B5, 2-8% galactosidase fermentation product filtrate, 0.1-2% b-glucan, 5-10% sodium hyaluronate, 10-20% glycerol, 0.5-3% tranexamic acid, 0.4-0.8% blue copper peptide, 0.5-1.5% arbutin, 1-3% nicotinamide, 0.8-1.2% bosine, 1-3% bifid yeast fermentation product, 1-3% bifid yeast fermentation lysate, and the balance water; The plant extract is prepared by mixing rosemary leaves, frankincense resin, tea tree flowers, sandalwood and jasmine flowers in a mass ratio of (3-8): (3-9): (1-3): (3-8): (1-3) and then subjecting the mixture to ultrasonic extraction.

2. The anti-aging moisturizing essence according to claim 1, characterized in that In percentage by mass, it includes 20% plant extract, 2% sodium p-hydroxybenzoate, 2% hexapeptide, 3% nicotinamide mononucleotide, 2% vitamin B5, 5% galactosidase fermentation product filtrate, 1% b-glucan, 7% sodium hyaluronate, 15% glycerol, 2% tranexamic acid, 0.6% blue copper peptide, 1% arbutin, 2% nicotinamide, 1% bosine, 2% bifid yeast fermentation product, 2% bifid yeast fermentation lysate, and the balance water; The plant extract is prepared by ultrasonic extraction after mixing rosemary leaves, frankincense resin, tea tree flowers, sandalwood and jasmine flowers in a mass ratio of 5:6:2:5:

2.

3. The anti-aging moisturizing essence according to claim 1 or 2, characterized in that: The anti-aging moisturizing essence also includes 5-6% seaweed fermentation product.

4. The anti-aging moisturizing essence according to claim 3, characterized in that The preparation of the seaweed fermentation product comprises: S1. Grind chlorella, spirulina, and antlers through an 80-mesh sieve; S2. Mix 2-8 parts of Chlorella, 2-8 parts of Spirulina, 5-10 parts of Antlers of Sea, and 5-15 parts of sucrose by weight, sterilize the mixture, inoculate with Kluyveromyces marxianus for fermentation, and then sterilize and filter to obtain a seaweed fermentation broth.

5. The anti-aging moisturizing essence according to claim 4, characterized in that: The inoculation amount of the Kluyveromyces marxianus is 3-7 wt %.

6. The anti-aging moisturizing essence according to claim 4, characterized in that The viable bacterial count of Kluyveromyces marxianus was 1×10 8 CFU / mL.

7. The anti-aging moisturizing essence according to claim 4, characterized in that: The fermentation temperature is 25-30° C., and the fermentation time is 75-85 hours.

8. A method for preparing the anti-aging moisturizing essence according to any one of claims 1 to 7, characterized in that: The preparation method comprises: Plant extract, sodium p-hydroxybenzoate, hexapeptide-3, nicotinamide mononucleotide, vitamin B5, galactosidase fermentation product filtrate, b-glucan, sodium hyaluronate, glycerin, tranexamic acid, blue copper peptide, arbutin, niacinamide, bosine, bifid yeast fermentation product, bifid yeast fermentation lysate, and water are added to a stirring pot and stirred at 40-60 rpm for 20-40 minutes to obtain an anti-aging moisturizing essence.

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