A whitening and anti-aging composition suitable for sensitive skin and application thereof

Abstract

The present application relates to a kind of whitening anti-aging composition suitable for sensitive skin and its application, belong to the technical field of cosmetics.The composition includes Psoralea corylifolia phenol, hydrolyzed royal jelly protein, radix notoginseng extract and camellia sinensis var. assamica flower extract as active ingredient.Psoralea corylifolia phenol has the effects of anti-aging, antioxidant and anti-inflammatory, etc.;Hydrolyzed royal jelly protein can help to relieve skin discomfort, moisturizing, enhance skin barrier function, etc.;Camellia sinensis var. assamica flower extract has the effects of anti-photoaging, whitening and anti-inflammatory soothing, while radix notoginseng extract has the effects of anti-inflammatory, antioxidant and anti-aging, etc.The composition of the present application can synergistically enhance the effects of anti-inflammatory, whitening and anti-aging by reasonable allocation of the above components, and the composition contains natural components, is mild and non-irritating, suitable for sensitive skin, and can be applied to various cosmetics.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetic technology, and particularly relates to a whitening and anti-aging composition suitable for sensitive skin and application thereof. BACKGROUND

[0002] With the increasing demand for fine skin care from consumers, skin care products with both whitening and anti-aging effects have become an important subfield in the cosmetic market. However, traditional whitening and anti-aging products are mostly designed for healthy skin, and their functional ingredients (such as high-concentration vitamin C, retinol, fruit acid, etc.) are often accompanied by strong irritation, which can easily cause adverse reactions such as redness, stinging, and peeling in sensitive skin. According to statistics, about 50% of consumers worldwide claim to have sensitive skin or occasional sensitive skin, and the demand for mild functional skin care products is particularly urgent among Asian populations due to their thin stratum corneum and relatively fragile skin barrier function.

[0003] In the prior art, the whitening and anti-aging solutions for sensitive skin mainly have the following defects:

[0004] The efficacy and mildness are difficult to balance. Traditional whitening ingredients (such as hydroquinone and kojic acid) and anti-aging ingredients (such as retinoic acid and its derivatives) exert their effects by inhibiting tyrosinase activity or promoting collagen regeneration, but their strong action mechanisms can easily damage the fragile skin barrier of sensitive skin, inducing inflammatory reactions. Although some products use low-concentration formulations or encapsulation technology to reduce irritation, the efficacy is significantly weakened, making it difficult to meet the needs of consumers for effects.

[0005] The ingredient synergy is insufficient. The existing technology mostly focuses on the optimization of single functional ingredients (such as nano-sized vitamin C derivatives and slow-release retinol), but lacks multi-target synergistic design of whitening and anti-aging pathways. For example, whitening ingredients may only act on the melanin production process, without simultaneously inhibiting oxidative stress or inflammatory mediators from damaging the skin; anti-aging ingredients may only promote collagen synthesis, without repairing DNA damage or basement membrane degradation caused by photoaging.

[0006] The compatibility of soothing systems and functional ingredients is defective. To alleviate irritation, some products add soothing ingredients such as bisabolol and ceramides, but such substances have poor compatibility with functional ingredients (such as vitamin C with acidic pH requirements), which can easily lead to inactivation of active substances or decrease in formulation stability. In addition, traditional soothing ingredients mostly focus on immediate calming, and lack long-term intervention for skin barrier repair.

[0007] There is a contradiction between permeability and safety. To improve the transdermal absorption of functional ingredients, the existing technology often uses chemical transdermal absorption enhancers (such as propylene glycol and ethanol) or physical penetration means (such as microneedles and iontophoresis), but such methods can further damage the stratum corneum structure of sensitive skin, exacerbating skin sensitivity.

[0008] The special physiological mechanism of sensitive skin is ignored, the characteristics of sensitive skin such as inflammatory response, increased trans-epidermal water loss rate (TEWL) and high nerve reactivity require that the whitening and anti-aging formula should consider anti-inflammatory, moisturizing and nerve calming functions. The formation of sensitive skin is mainly related to the damage of skin barrier function. Excessive cleaning, frequent exfoliation, use of irritating cosmetics or skin care products, and long-term exposure to environmental factors such as pollution and ultraviolet rays can all damage the skin barrier, leading to water loss and easy invasion of external irritants, thereby causing redness, stinging, itching and other sensitive symptoms.

[0009] In view of the detailed needs of sensitive skin for skin care products, it is urgent to develop a whitening and anti-aging composition suitable for sensitive skin. SUMMARY

[0010] The present application aims to overcome the shortcomings of the prior art and provide a whitening and anti-aging composition suitable for sensitive skin and its application. The composition provided by the present application can significantly improve the dullness of sensitive skin, has a significant whitening and anti-aging effect, is mild and non-irritating, has multiple functions such as anti-photoaging, moisturizing, anti-inflammatory and soothing, is suitable for long-term use on sensitive skin, and can protect and repair the skin barrier.

[0011] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows:

[0012] In a first aspect, the present application provides a whitening and anti-aging composition suitable for sensitive skin, which comprises as active ingredients psoralen, hydrolyzed royal jelly protein, radix notoginseng extract and camellia nitidissima flower extract.

[0013] Preferably, the weight ratio of psoralen, hydrolyzed royal jelly protein, radix notoginseng extract and camellia nitidissima flower extract in the composition provided in the first aspect is (5-8):(10-15):(15-20):(10-20).

[0014] Further preferably, the weight ratio of psoralen, hydrolyzed royal jelly protein, radix notoginseng extract and camellia nitidissima flower extract in the composition provided in the first aspect is (6-8):(11-14):(16-18):(14-16), and further preferably 7:12:16:15.

[0015] Preferably, the preparation method of the camellia nitidissima flower extract comprises the following steps: mixing camellia nitidissima flower powder with an ethanol aqueous solution with a volume fraction of 55-75%, ultrasonic extraction at 30-45℃ for 0.5-1h, ultrasonic power 80-120W, filtering, concentrating and freeze-drying after extraction to obtain camellia nitidissima flower extract, and the solid-liquid ratio of the camellia nitidissima flower powder to the ethanol aqueous solution is 1g:(10-20)mL.

[0016] In a second aspect, the present application further provides a preparation method of the whitening and anti-aging composition suitable for sensitive skin, comprising: mixing the components to obtain the composition.

[0017] In a third aspect, the present application further provides use of the whitening and anti-aging composition suitable for sensitive skin in the preparation of a cosmetic.

[0018] In a fourth aspect, the present application further provides a cosmetic containing the whitening and anti-aging composition suitable for sensitive skin according to the first aspect.

[0019] Preferably, the cosmetic according to the fourth aspect further comprises a cosmetically acceptable adjuvant, which comprises at least one of emulsifiers, humectants, thickening agents, preservatives, flavoring agents, and transdermal absorption enhancers.

[0020] Preferably, the cosmetic according to the fourth aspect contains the whitening and anti-aging composition according to the first aspect in an amount of 0.1-10 wt%.

[0021] More preferably, the cosmetic is any one of water, serum, essence, mask liquid, or cream.

[0022] Bakuchiol (CAS No. 10309-37-2) is a natural terpenoid compound extracted from the seeds of Psoralea corylifolia, a legume, belonging to isoprenyl phenol derivatives. Its chemical structure is unique, with a molecular formula of C 18 H 24 O and a molecular weight of 256.38, and it is easily oxidized. Bakuchiol can stimulate the synthesis of collagen type I and other collagens in the dermis by activating retinol receptors, improve skin elasticity, and reduce wrinkles. It can also inhibit the activity of matrix metalloproteinases (MMP-1, MMP-3), reduce collagen degradation, and improve wrinkle depth. Clinical studies have shown that 0.5% bakuchiol cream can improve the effects of photodamaged skin within 12 weeks, comparable to retinol. Bakuchiol can promote healthy metabolism of the epidermal keratin layer, smooth the skin, and has better tolerance, without side effects such as stinging and peeling, making it suitable for sensitive skin and anti-aging populations.

[0023] Hydrolyzed royal jelly protein is a small molecule peptide and amino acid complex obtained by enzymatic treatment of royal jelly, which has the nutritional activity of royal jelly and better skin permeability. By hydrolyzing the large molecular weight proteins in royal jelly into low molecular weight peptides with protease, the transdermal absorption efficiency is significantly improved. The core active ingredients of royal jelly (such as royal jelly acid, amino acid, vitamin) are retained, but the problems of poor stability and easy sensitization of natural royal jelly are avoided. It promotes collagen regeneration and anti-aging, stimulates fibroblast proliferation, up-regulates collagen (I / III type) and elastic fiber synthesis gene expression; anti-inflammatory and soothing, stimulating fibroblast proliferation, up-regulating collagen (I / III type) and elastic fiber synthesis gene expression, inhibiting histamine release, relieving lactic acid-mediated stinging reaction (suitable for sensitive skin), blocking the MAPK / NF-κB inflammatory signaling pathway, reducing the release of TNF-α, IL-6 and other pro-inflammatory factors. Antioxidant, rich in superoxide dismutase and small molecule peptides, can effectively clear ROS free radicals, reduce lipid peroxidation, and maintain the integrity of the stratum corneum lipid barrier; the hydrolysate is rich in hydrophilic amino acids such as proline and lysine, which can improve the hydration capacity of the stratum corneum and reduce the trans-epidermal water loss rate (TEWL); indirectly inhibits tyrosinase activity (by down-regulating MITF transcription factor), reduces melanin synthesis, and the effect is better than that of kojic acid.

[0024] Radix pseudostellariae extract is derived from the dried tuber of Pseudostellaria heterophylla of the family Caryophyllaceae. The main active ingredients include triterpenoid saponins (such as pseudostellaria saponin A, with antiviral and anti-inflammatory effects), polyphenols and polysaccharides (such as radix pseudostellariae polysaccharide, which can enhance immunity and lower blood sugar), flavonoids (such as radix pseudostellariae flavone, with antioxidant and antibacterial activity). Anti-photoaging, improving skin SOD activity, reducing lipid peroxidation product MDA, increasing collagen marker hydroxyproline content. Whitening and freckle lightening, can inhibit tyrosinase activity, reduce melanin synthesis, and synergistically reduce oxidative stress-induced pigment deposition.

[0025] Camellia sinensis var. assamica flower extract contains rich active ingredients such as polyphenols (such as flavanols, phenolic acids), flavonoids (such as quercetin, kaempferol), saponins, polysaccharides, amino acids, and volatile oils, which can exert strong antioxidant and anti-inflammatory effects, regulate skin immunity, promote cell renewal, form a moisturizing film to repair the skin barrier, and reduce sensitive symptoms such as redness and itching.

[0026] Compared with the prior art, the present application has the following advantages:

[0027] Sensitive skin is prone to dryness, redness, itching, allergy and other common problems caused by different substances, so special cosmetics are needed to improve the skin condition. The present application provides a composition of bakuchiol, hydrolyzed royal jelly protein, radix notoginseng extract and camellia nitidissima flower extract, which has the effects of anti-inflammatory soothing, antioxidant, whitening, skin barrier repair, anti-aging and the like. The composition is compounded by specific components and amounts, so that each component can fully exert its effect, and can form a synergistic effect with each other. The related experiments of specific embodiments prove that the composition can effectively achieve the skin care effect through the effects of anti-inflammatory soothing, antioxidant, whitening, anti-aging and the like, and is suitable for sensitive skin.

[0028] The composition of the present application uses natural and mild plant extracts as raw materials, so that the composition is mild and non-irritating while having significant efficacy, is suitable for sensitive skin, and can be applied to various cosmetics, and has good market application prospect.

[0029] Drawings of the specification

[0030] Figure 1 Figure of slight irritation of the skin stimulation test of the low dose group of comparative example 5 on 3 nude mice. DETAILED DESCRIPTION

[0031] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific embodiments.

[0032] It should be understood that the present application is not limited to the specific compositions, methods or schemes described herein. In addition, unless otherwise defined, all technical and scientific terms used herein have the same meaning as generally understood by one of ordinary skill in the art to which this application belongs. It should also be understood that the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the scope of the present application, which is defined only by the claims.

[0033] The experimental methods in the following examples not specified in the specific conditions are generally carried out according to the conventional conditions, or according to the conditions recommended by the manufacturer. The materials, reagents or instruments used, unless otherwise specified, can be obtained from commercial channels.

[0034] Some raw materials and sources are as follows:

[0035] Bakuchiol: purchased from Nanjing Longze Pharmaceutical and Chemical Co., Ltd.

[0036] Hydrolyzed royal jelly protein: purchased from Baoding Fangsheng Biological Development Co., Ltd.

[0037] Radix notoginseng extract: purchased from Gansu Yishengxiang Biological Technology Co., Ltd.

[0038] Centella asiatica extract: purchased from Gansu Yishengxiang Biological Technology Co., Ltd.

[0039] Jinyinhua extract: purchased from Gansu Yishengxiang Biotechnology Co., Ltd.;

[0040] Hydrolyzed collagen: purchased from Dongguan Hengyi Biotechnology Co., Ltd.

[0041] Jinhuacha flower extract: self-made, the preparation method is as follows: collect Jinhuacha flowers, wash and dry in a 60℃ constant temperature oven until the weight is constant, grind and crush to pass through a 100 mesh sieve, collect the undersize, and obtain Jinhuacha flower powder; add 70% ethanol aqueous solution by volume fraction to Jinhuacha flower powder at a material-liquid ratio of 1g:15mL, mix well, ultrasonic extract at 40℃ for 30min, the ultrasonic power is 100W, filter the filtrate after extraction, and concentrate the filtrate at 50℃ by rotary evaporation, then freeze-dry in an ultra-low temperature freeze dryer to obtain Jinhuacha flower extract.

[0042] Example 1

[0043] A whitening and anti-aging composition suitable for sensitive skin, the composition comprises bakuchiol, hydrolyzed royal jelly protein, Taizishen extract and Jinhuacha flower extract, and the weight ratio of each component is 7:12:16:15.

[0044] Example 2

[0045] A whitening and anti-aging composition suitable for sensitive skin, the composition comprises bakuchiol, hydrolyzed royal jelly protein, Taizishen extract and Jinhuacha flower extract, and the weight ratio of each component is 6:14:17:13.

[0046] Example 3

[0047] A whitening and anti-aging composition suitable for sensitive skin, the composition comprises bakuchiol, hydrolyzed royal jelly protein, Taizishen extract and Jinhuacha flower extract, and the weight ratio of each component is 7:13:16:16.

[0048] Example 4

[0049] A whitening and anti-aging composition suitable for sensitive skin, the composition comprises bakuchiol, hydrolyzed royal jelly protein, Taizishen extract and Jinhuacha flower extract, and the weight ratio of each component is 8:11:18:18.

[0050] Comparative Example 1

[0051] A whitening and anti-aging composition suitable for sensitive skin, the composition comprises hydrolyzed royal jelly protein, Taizishen extract and Jinhuacha flower extract, and the weight ratio of each component is 12:16:15; compared with Example 1, the component bakuchiol is reduced.

[0052] Comparative Example 2

[0053] A whitening and anti-aging composition suitable for sensitive skin, the composition comprising psoralen, radix pseudostellariae extract and lutea flower extract, the weight ratio of each component being 7:16:15; compared with Example 1, the component hydrolyzed royal jelly protein is reduced.

[0054] Comparative Example 3

[0055] A whitening and anti-aging composition suitable for sensitive skin, the composition comprising psoralen, hydrolyzed royal jelly protein and lutea flower extract, the weight ratio of each component being 7:12:15; compared with Example 1, the component radix pseudostellariae extract is reduced.

[0056] Comparative Example 4

[0057] A whitening and anti-aging composition suitable for sensitive skin, the composition comprising psoralen, hydrolyzed royal jelly protein and radix pseudostellariae extract, the weight ratio of each component being 7:12:16; compared with Example 1, the component lutea flower extract is reduced.

[0058] Comparative Example 5

[0059] The difference compared with Example 1 is only that an equal amount of retinol replaces psoralen in the composition of Comparative Example 5.

[0060] Comparative Example 6

[0061] The difference compared with Example 1 is only that an equal amount of hydrolyzed collagen replaces hydrolyzed royal jelly protein in the composition of Comparative Example 6.

[0062] Comparative Example 7

[0063] The difference compared with Example 1 is only that an equal amount of centella asiatica extract replaces radix pseudostellariae extract in the composition of Comparative Example 7.

[0064] Comparative Example 8

[0065] The difference compared with Example 1 is only that an equal amount of honeysuckle extract replaces lutea flower extract in the composition of Comparative Example 8.

[0066] Comparative Example 9

[0067] A whitening and anti-aging composition suitable for sensitive skin, the composition comprising psoralen, hydrolyzed royal jelly protein, radix pseudostellariae extract and lutea flower extract, the weight ratio of each component being 3:16:10:25.

[0068] Comparative Example 10

[0069] A whitening and anti-aging composition suitable for sensitive skin, the composition comprising psoralen, hydrolyzed royal jelly protein, radix pseudostellariae extract and lutea flower extract, the weight ratio of each component being 7:8:25:7.

[0070] Table 1 Formulation table of Examples 1-4, Comparative Examples 1-10 (parts by weight)

[0071]

[0072]

[0073] Performance test verification of Examples 1-4 and Comparative Examples 1-10

[0074] 1. Skin irritation test:

[0075] The whitening and anti-aging compositions of Examples 1-4 and Comparative Examples 1-10 suitable for sensitive skin were configured into 0.05 g / mL, 0.01 g / mL, and 0.15 g / mL deionized water solutions of low, medium, and high concentrations for the skin irritation test. The test subjects were BALB / c nude mice, 8 mice per group (5 male and 5 female mice were randomly selected), and 50 μL was applied to the back of the mice (0.5 cm x 0.5 cm). The application was performed twice a day, in the morning and in the evening (with an interval of 8 hours). The mice behavior and skin condition were observed every day for 4 consecutive weeks. The scoring was performed according to the following table standards:

[0076] Mouse skin condition Score / points Normal skin 5 Mild irritation (reddish, slight red rash, chapped, peeling, etc.) 3 Red rash, edema, ulcer, blister, scratching 2 Poor activity, death 0

[0077] Table 2 Results of the skin irritation test after 4 weeks

[0078]

[0079]

[0080]

[0081] As shown in the above table, after 4 weeks of skin irritation test, the low, medium, and high concentration samples of the whitening and anti-aging compositions of Examples 1-4 did not produce irritation to the mice, and did not cause mild irritation such as redness, mild rash, dryness, and peeling of the mouse skin. This indicates that the whitening and anti-aging compositions of Examples 1-4 have good soothing properties. However, compared with Example 1, Comparative Example 5 used retinol instead of bakuchiol, and the low, medium, and high concentration groups of Example 5 showed different degrees of skin irritation. In the low concentration group, 3 mice showed skin redness (see the attached drawing Figure 1 ), and in the medium and high concentration groups, 6 and 8 mice, respectively, showed red rash and different degrees of scratching.

[0082] 2. Anti-inflammatory performance test

[0083] Experimental materials: The cell line used was RAW264.7 cells in the logarithmic growth phase (Wuhan Sino Biological Technology Co., Ltd.), and the TNF-α, IL-6 inflammatory factor detection kit (Abeam).

[0084] The specific test method is as follows:

[0085] (1) Inoculation: inoculate cells into culture bottles, 24-well culture plates, and incubate at 37°C in a 5% CO2 incubator for 24 h with a culture density of 1 x 10 6

[0086] (2) Modeling:

[0087] Control group: remove the supernatant in the 24-well plate, 6 holes in parallel, add 0.5 μL of DMEM culture solution and 0.1 μL of PBS solution to each hole;

[0088] Lipopolysaccharide (LPS) verification model group: remove the supernatant in the 24-well plate, 6 holes in parallel, add 500 μL of DMEM culture solution and 100 μL of LPS solution to each hole.

[0089] Experimental group: remove the supernatant in the 24-well plate, 6 holes in parallel, add 500 μL of DMEM culture solution, 100 μL of LPS and a mixed solution of samples (example and comparative example samples) to each hole.

[0090] (3) Detection: collect the supernatant after 24 h of continued incubation at 37°C in a 5% CO2 incubator, centrifuge the sample in a centrifuge tube to obtain the supernatant for inflammation factor concentration detection. Calculation: inflammation factor inhibition rate (%) = [(model group inflammation factor average content - treatment group inflammation factor average content) / (model group inflammation factor average content - blank control inflammation factor average content)] x 100%.

[0091] Table 3 Inflammation factor inhibition rate

[0092]

[0093]

[0094] ​From the results of Table 3, it can be seen that: 1) the inhibition rates of the whitening and anti-aging compositions of Examples 1-4 on inflammatory factors TNF-α and IL-6 were 85.6%-89.4% and 81.4%-84.1% respectively, indicating that the compositions had significant anti-inflammatory and soothing effects; 2) comparing the experimental results of Comparative Example 1 with Comparative Examples 1-4, it can be seen that the combination of the four components contained in the examples can achieve anti-inflammatory and soothing effects, and after reducing a specific component, the inhibition rates on inflammatory factors TNF-α and IL-6 were reduced to 37.7%-55.9% and 42.7%-49.8% respectively, and the anti-inflammatory effect of the composition was significantly reduced; 3) compared with Example 1, Comparative Examples 5-8 replaced a component in the whitening and anti-aging composition with a functional analogue, and the inhibition rates on inflammatory factors TNF-α and IL-6 were reduced to 42.7%-56.4% and 43.9%-53.1% respectively; 4) compared with Example 1, Comparative Examples 9 and 10 adjusted the weight ratio of the components in the composition, which significantly reduced the inhibition rates on inflammatory factors TNF-α and IL-6. The above experimental results show that the bakuchiol, hydrolyzed royal jelly protein, radix notoginseng extract and lamiophloma violascens flower extract in the composition of the application can play a synergistic effect in anti-inflammatory and soothing effects, and the compounding of similar functional components cannot achieve the same or similar anti-inflammatory and soothing effects. At the same time, the amount ratio of the four components in the application also has a significant influence on the anti-inflammatory and soothing effects.

[0095] 3. Anti-aging performance test

[0096] The whitening and anti-aging compositions of Examples 1-4 and Comparative Examples 1-10 were subjected to DPPH free radical scavenging test.

[0097] Test method: 4.0 mL of 1×10 -4 mol / L DPPH solution and 0.5 mL of sample deionized water solution (0.01 g / mL) of the same concentration were sequentially added to a 10 mL test tube, which was sealed with a sealing film and shaken to mix, and the absorbance A1 at 517 nm was immediately detected. After 30 min of reaction in the dark, the absorbance A2 at 517 nm was detected. 4.0 mL of DPPH solution and 0.5 mL of sample diluent (deionized water) were added to the test tube, which was shaken to mix, and the absorbance value was determined, which was recorded as A0.

[0098] DPPH free radical scavenging rate: Y = [1-(A2-A1) / A0]×100%.

[0099] Table 4 DPPH free radical scavenging rate

[0100] Group DPPH radical scavenging rate (%) Example 1 93.5％ Example 2 88.7％ Example 3 89.4％ Example 4 93.2％ Comparative Example 1 58.3％ Comparative Example 2 67.1％ Comparative Example 3 72.0％ Comparative Example 4 73.9％ Comparative Example 5 63.4％ Comparative Example 6 71.7％ Comparative Example 7 76.6％ Comparative Example 8 74.5％ Comparative Example 9 81.4％ Comparative Example 10 79.3％

[0101] According to the results in Table 4, it can be seen that the whitening and anti-aging compositions of Examples 1-4 have significant anti-aging effects, with DPPH free radical scavenging rates ranging from 88.7% to 93.5%, indicating significant anti-aging effects. By comparing the experimental results of Comparative Examples 1-10 and Comparative Example 1, it can be seen that the bakuchiol, hydrolyzed royal jelly protein, radix notoginseng extract, and Lamiophloma violascens flower extract in the compositions of the present application can play a synergistic effect in anti-aging, and it is difficult to achieve the same or similar effect by compounding similar functional components or changing the amount ratio of the four components.

[0102] Application Examples

[0103] The skin care emulsion of the application examples 1-4 and comparative application examples 1-10 has the components (mass percentage) as shown in Table 5; wherein the application examples 1-4 and comparative application examples 1-10 respectively use the whitening and anti-aging compositions suitable for sensitive skin prepared in Examples 1-4 and Comparative Examples 1-10.

[0104] Table 5: Skin care emulsion ingredient table

[0105]

[0106] The preparation method of the skin care emulsion comprises the following steps:

[0107] Sterile deionized water is heated to 50℃, and carbomer and sodium hyaluronate are added and stirred until completely dissolved; then arginine and the whitening and anti-aging composition provided in the examples or comparative examples are added and stirred; then glycerol, propylene glycol, cocoglycoside, and glycerol glucoside are added and stirred, and the mixture is homogenized and emulsified by a high-pressure homogenizer, cooled to room temperature, discharged, and the skin care emulsion is obtained.

[0108] 1. Human patch test of the skin care emulsion of the application examples

[0109] Experimental method: human patch test is used to verify the irritation of the application examples 1-4 to sensitive skin, and the specific test method refers to the “Cosmetic Safety Technical Specification” (2015 edition), and the skin adverse reaction grading standard is shown in Table 6.

[0110] Forty volunteers are recruited, half of whom are male and half of whom are female, aged 19-55 years old, with an average age of 32 years old. First, select the appropriate patch tester, and use the closed patch test method to apply an equal amount of about 25 μL of the test substance to the patch tester, and apply it to the arm of the volunteer with low sensitization tape, and lightly press it to evenly apply it to the skin. After 24 hours, remove the test substance, and observe the skin reaction at 0.5, 24, and 48 hours after removing the patch tester. The skin adverse reaction recording results are shown in Table 7.

[0111] Table 6: Skin adverse reaction grading standard

[0112]

[0113] Table 7 Results of recording adverse reactions of skin

[0114]

[0115]

[0116] The patch test results show that no adverse reactions of skin occur in application examples 1-4, which is consistent with the results of the skin irritation test in Table 2 after 4 weeks, indicating that the whitening and anti-aging composition suitable for sensitive skin prepared in examples 1-4 and the skin care emulsions prepared in application examples 1-4 have good skin affinity, no irritation, and no sensitization.

[0117] 2. Whitening performance test of application examples

[0118] 100 volunteers aged 20 to 50 years old with whitening needs were selected as subjects and randomly divided into 10 groups, 10 people in each group. Each group used the skin care emulsions prepared in application examples 1-4 and comparative application examples 1-10. The subjects applied the skin care emulsions to the face twice a day, morning and evening. The test lasted for 4 weeks. The skin red and black pigment tester Hexameter MX18 was used to evaluate the change of skin melanin content before and after the subjects used the skin care emulsions. The average melanin content before use was recorded as M1, and the average melanin content after 4 weeks of use was recorded as M2. The melanin inhibition rate T = (M1-M2) / M1x100%.

[0119] Table 8 Results of melanin inhibition rate

[0120] Group Melanin inhibition rate T (%) Application Example 1 31.71％ Application Example 2 28.63％ Application Example 3 29.44％ Application Example 4 30.58％ Comparative Application Example 1 7.46％ Comparative Application Example 2 9.59％ Comparative Application Example 3 10.95％ Comparative Application Example 4 11.38％ Comparative Application Example 5 14.54％ Comparative Application Example 6 16.57％ Comparative Application Example 7 15.60％ Comparative Application Example 8 14.55％ Comparative Application Example 9 16.74％ Comparative Application Example 10 17.36％

[0121] According to the results in Table 8, the skin care emulsions in application examples 1-4 can significantly and effectively reduce the melanin inhibition rate compared to comparative application examples 1-4, which indicates that the whitening and anti-aging composition suitable for sensitive skin in examples 1-4 has significant whitening efficacy.

[0122] The preferred embodiments of the present application disclosed above are only used to help explain the present application. The preferred embodiments do not describe all the details, nor limit the application to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of the present application. The present application selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present application, so that those skilled in the art can well understand and utilize the present application. The present application is limited only by the claims and their full scope and equivalents.

Claims

1. A whitening anti-aging composition suitable for sensitive skin, characterized in that, The composition is composed of bakuchiol, hydrolyzed royal jelly protein, radix notoginseng extract and camellia sinensis var. assamica flower extract; the weight ratio of the bakuchiol, hydrolyzed royal jelly protein, radix notoginseng extract and camellia sinensis var. assamica flower extract is (5-8):(10-15):(15-20):(10-20). 2.The whitening and anti-aging composition suitable for sensitive skin according to claim 1, characterized in that, The weight ratio of the bakuchiol, hydrolyzed royal jelly protein, radix notoginseng extract and camellia sinensis var. assamica flower extract is (6-8):(11-14):(16-18):(14-16).

3. The whitening and anti-aging composition for sensitive skin according to any one of claims 1-2, characterized in that, The preparation method of the camellia sinensis var. assamica flower extract comprises the following steps: mixing camellia sinensis var. assamica flower powder with ethanol aqueous solution, ultrasonic extraction, filtering, concentrating and freeze-drying after the extraction to obtain the camellia sinensis var. assamica flower extract.

4. The method for preparing the whitening and anti-aging composition for sensitive skin according to any one of claims 1 to 3, characterized in that, The method comprises the following steps: mixing the components to obtain the composition.

5. A cosmetic product, characterized by, The cosmetic comprises the whitening and anti-aging composition suitable for sensitive skin according to any one of claims 1-3.

6. The cosmetic product according to claim 5, characterized in that, The cosmetic further comprises cosmetically acceptable adjuvants, which include at least one of emulsifiers, humectants, thickening agents, preservatives, fragrances and transdermal absorption promoters.

7. The cosmetic product according to claim 5, characterized in that, The whitening and anti-aging composition is added in the cosmetic in an amount of 0.1-10wt%.

8. The cosmetic product according to any one of claims 5 to 7, characterized in that, The cosmetic is any one of water, essence, essence cream, mask liquid or cream.

9. Use of the whitening and anti-aging composition suitable for sensitive skin according to any one of claims 1-3 in the preparation of a cosmetic.

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