Primers and digital PCR kit for detecting infectious endocarditis pathogens

Abstract

The invention relates to the technical field of detection, in particular to primers and a digital PCR kit for detecting infectious endocarditis pathogens. The invention provides a primer probe combination. The primer probe combination comprises any nucleotide sequence as shown in SEQ ID No. 1 to SEQ ID No. 39. The kit is mainly used for detecting 12 pathogens capable of causing infectious endocarditis, namely streptococcus, staphylococcus aureus, coagulase negative staphylococcus, enterococcus, candida albicans, pseudomonas aeruginosa, pseudomonas maltophilia, escherichia coli, klebsiella pneumoniae, acinetobacter baumannii, rickettsia and bartonella. Four detection channels and one internal control channel can be detected at a time, 12 irrelevant human common pathogens are not subjected to non-specific amplification, and absolute quantification can be carried out.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to primers and a digital PCR kit for detecting infectious endocarditis pathogens. Background technique [0002] Infective endocarditis (IE) refers to a series of fatal diseases characterized by valve involvement caused by pathogens directly invading heart valves. The main clinical manifestations of active infective endocarditis (AIE) are hemodynamic disturbance, uncontrolled sepsis, paravalvular abscess, cerebral aneurysm or cerebral hemorrhage, or large vegetation. The early symptoms of patients with infective endocarditis lack specificity, and the first visit may occur in multiple departments. Traditional or modified Duke criteria are usually used to diagnose patients with suspected IE. In recent years, the incidence of IE has shown a significant increase trend, especially among the elderly and hospitalized patients. The risk factors mainly include bacterial drug resistance, ar...

AI Technical Summary

Problems solved by technology

[0006] (1) The sensitivity of fluorescent quantitative PCR is lower than that of digital PCR, which may cause missed detection and false negatives;

[0007] (2) Fluorescent quantitative PCR usually can only detect two fluorescent channels at the same time, and cannot take into account the internal control channel; or two copies, that is, two genes and one internal control gene are used to form the detection system;

[0008] (3) The quantification of copy number by fluorescent quantitative PCR depends on the standard curve, and the samples with unknown concentration are estimated from the known concentration standard, which does not achieve true absolute quantification

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