Nucleic acid extraction pretreatment amplification system

A pretreatment, nucleic acid technology, applied in the fields of biological material pretreatment, biomass post-processing, biochemical instruments, etc., can solve the problems of complex pretreatment and preparation process, unsuitable for rapid detection, and high technical cost, and achieve easy implementation and operation. , The effect of saving labor time and reducing the overall volume

Active Publication Date: 2020-03-06
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] 1. The temperature cycle adopted requires a complex temperature control system to realize, and the technical cost is relatively high;
[0005] 2. The volume of the whole machine is relatively large, which is not suitable for rapid ...

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  • Nucleic acid extraction pretreatment amplification system
  • Nucleic acid extraction pretreatment amplification system
  • Nucleic acid extraction pretreatment amplification system

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Embodiment Construction

[0033] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0034] The core of the present invention is to provide a nucleic acid extraction, pretreatment and amplification system with simple structure, small size and low cost.

[0035] Please refer to Figure 1 to Figure 5 , figure 1 It is a schematic diagram of a specific embodiment of the nucleic acid extraction pretreatment amplification system provided by the present invention; figure 2 is a schematic diagram of the filter device; image 3 is a schematic diagr...

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Abstract

The invention discloses a nucleic acid extraction pretreatment amplification system. The system comprises a filtering device, a DNA extraction device, a uniform mixing device, a PCR amplification device and a driving device for driving a liquid to flow. The labor time is saved and the working efficiency of people is improved through a method of sequentially treating samples by different reagents.Through ingenious pipeline design, functional units such as the filtering device, the DNA extraction device for nucleic acid extraction, the uniform mixing device and the PCR amplification device areintegrated in an embedded manner, so that the overall size is remarkably reduced. The system has the advantages of being simple in structure, easy to achieve and operate, low in cost and capable of being widely used and popularized. Besides, the PCR amplification device is based on temperature circulation, so that rapid amplification and signal amplification of polymerase on nucleic acid moleculesare realized, and the detection time is greatly shortened. In addition, measurable reagents are wide in variety, for example, bacteria, viruses, fungi, chlamydia, rickettsia and the like can be rapidly detected.

Description

technical field [0001] The invention relates to the technical field of accounting, extraction and amplification, and more specifically, to a nucleic acid extraction, pretreatment and amplification system. Background technique [0002] Quantitative PCR is the most important molecular biology detection method today, which can quickly identify bacteria, viruses, fungi and other microbial types and drug-resistant mutations, and the analysis sensitivity is far higher than other detection methods; it only takes 30 to 40 minutes. It can accurately detect the number of different microbial populations, so it has important application prospects in almost all life science fields such as food testing, clinical testing, disease control, inspection and quarantine, scientific research laboratories, food safety, cosmetics testing, and environmental hygiene. [0003] The existing PCR amplification instrument mainly has the following defects: [0004] 1. The temperature cycle adopted require...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/36C12M1/12
CPCB01L7/52B01L2300/1805
Inventor 吴文明
Owner CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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