Gene chip for detecting various prawn pathogenes and detection method thereof

Abstract

The invention relates to a gene chip for detecting various prawn pathogenes and a detection method thereof. The gene chip is prepared by arranging the probes of a quality control system and the probe points of 18 types of prawn pathogenes on the same solid-phase carrier nylon membrane. The probes of 18 types of the prawn pathogenes comprise probes for detecting 15 types of viruses, probes for detecting fusarium, fish-killing aphanomyces and deformation algal filament cristatum, and probes for detecting Paranophrys and prawn rickettsia. When the gene chip is used for detection, a signal is judged according to the condition whether blue or royal purple appears at a hybridization point by using a digoxin hybridization chromogenic method. The construction of the quality control system on the gene chip ensures the detection specificity and sensitivity, thus, the gene chip can be used for simultaneously detecting various prawn pathogenic microorganisms on the same nylon membrane, has the characteristic of high flux, can replace the prior related detection method for the prawn pathogenic microorganisms, and is a breakthrough of the prior prawn pathogenic microorganism judging technique.

Description

technical field [0001] The invention belongs to the technical field of pathogen detection of marine organisms using a gene chip, and in particular relates to a gene chip for detecting various prawn pathogens and a detection method thereof. Background technique [0002] In recent years, the use of nucleic acid technology to detect pathogenic microorganisms in marine animals has developed very rapidly. Combined with immunology technology, restriction enzyme technology and loop-mediated isothermal amplification and other methods. However, due to the variety of farmed animals and the variety of pathogens, the detection and diagnosis of a single pathogen cannot fully reflect the cause and status of the disease, which is not conducive to determining effective control measures. Existing pathogen detection techniques have large limitations, low efficiency, and poor systematization. Therefore, there is an urgent need for breakthroughs in high-throughput analysis capabilities in mari...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a gene chip and detection method for detecting various prawn pathogens, for detecting white spot syndrome virus, infectious subcutaneous and hematopoietic organ necrosis virus, hepatopancreatic parvovirus, Penaeus monodon baculovirus, Taura virus, Yellow head virus, Shrimp baculovirus, Spawning shrimp death virus, Infectious myonecrosis virus, LSNV, Maori virus, Shrimp nodavirus, NHP, Fusarium, Ichthyophthora, Proteus A total of 18 types of pathogens of prawns including Bacillus, Pseudomonas and Rickettsia prawns, to solve the problems of high-throughput detection, low sensitivity and poor accuracy of existing technologies

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