Method for removing heavy metal in different forms in polluted soil through acidophilic bacteria leaching

A technology for contaminated soil and heavy metals, applied in the field of microbial remediation, can solve the problems of slow remediation process, low treatment efficiency, poor environmental adaptability, etc., and achieve the effect of low operating cost and simple operation

Inactive Publication Date: 2019-12-03
SHANGHAI SECOND POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When remediating heavy metal pollution, most plants can only enrich 1 or 2 kinds of heavy metals, and the remediation process is slow; animal remediation animal species are very limited, poor environmental adaptability, only suitable for low-concentration heavy metal contaminated soil, and the treatment efficiency Low; microbia

Method used

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  • Method for removing heavy metal in different forms in polluted soil through acidophilic bacteria leaching
  • Method for removing heavy metal in different forms in polluted soil through acidophilic bacteria leaching
  • Method for removing heavy metal in different forms in polluted soil through acidophilic bacteria leaching

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Comparison of the removal effects of strains on heavy metals in soil before and after domestication:

[0035] (1) Strain domestication

[0036] (2) Leachate (medium) components: (NH 4 ) 2 SO 4 : 3g / L, K 2 HPO 4 : 0.5g / L, MgSO 4 ·7H 2 O: 0.5g / L, KCl: 0.5g / L, Ca(NO 3 ) 2 : 0.01g / L, FeSO 4 ·7H 2 O: 40g / L, pH value is 2, leaching culture is carried out at 30°C and 120rmp / min.

[0037] (3) Add 2% (v / v) strains before and after domestication to the leaching device, put the leaching device into a constant temperature shaker incubator, and leaching for 8 days at 30°C and 120rmp / min, the leachate The amount of soil in the medium is 5g / L, finally analyze the content change of heavy metal in the leachate, the result is as follows figure 2 As shown, the removal effect of acidophilus on various heavy metals was improved after domestication, the removal rate of Zn increased from 53.9% to 77.2%, the removal rate of Cu increased from 20.5% to 55.6%, and the removal rate of...

Embodiment 2

[0039] Changes of heavy metal content in leachate during acidophilus leaching process:

[0040] Set different single factor conditions (solid-liquid ratio, inoculum size, initial pH, FeSO 4 ·7H 2 O content), the leaching device was placed in a constant temperature shaking incubator, and leached at 28-35°C and 120rmp / min for 12 days. From the first day, a 1ml sample was taken quantitatively every day. image 3 For the leachate (medium) component: (NH 4 ) 2 SO 4 : 3g / L, K 2 HPO 4 : 0.5g / L, MgSO 4 ·7H 2 O: 0.5g / L, KCl: 0.5g / L, Ca(NO 3 ) 2 : 0.01g / L, FeSO 4 ·7H 2 O: 40g / L, pH value 2, solid-liquid ratio 100:1, inoculum size 1% (v / v), leaching culture at 30°C, 120rmp / min for 12d. Each group has 3 parallels, and a control CK (not inoculated with strains). The results show the changes in the removal rates of heavy metals Zn, Cu, Ni, and Cd under different leaching culture times. It can be seen that as the leaching time becomes longer, the removal rates of Zn, Cu, Ni, and...

Embodiment 3

[0042] When the inoculation amount is 1% (v / v), acidophilus changes the form of heavy metals in soil:

[0043] The example operation steps are the same as in Example 1, about 10 days of leaching, the soil after the leaching is collected, and the change situation of its heavy metal form is detected, the results are as follows Figure 4 shown. Compared with the soil before leaching, the contents of various forms of heavy metals in the soil after leaching were significantly reduced. Compared with the control CK group not inoculated with bacteria, it can be seen that the stable oxidizable state and residue state are transformed into the exchangeable or reducible state that is easier to remove under the action of acidophilus, in which the heavy metal Cd After filtration, each heavy metal form could not be detected because the content was too low. It can effectively remove 80% to 95% of the exchangeable state, 75% to 90% of the reducible state, 70% to 90% of the oxidizable state a...

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Abstract

The invention discloses a method for removing heavy metal in different forms in polluted soil through acidophilic bacteria leaching. The method comprises the following steps that firstly, strains areacclimatized, then the acclimatized strains are inoculated into a culture solution containing the heavy metal highly-polluted soil, leaching culture is conducted under certain parameter conditions, and by changing the occurrence form of the heavy metal in the soil, the heavy metal highly-polluted soil is repaired. The method utilizes acidithiobacillus ferrooxidans to remove the heavy metal in different forms in the heavy metal highly-polluted soil, and has the advantages that operation is easy, the cost is low, the repairing effect is good, a bacteria solution can be secondarily utilized, andsecondary pollution to the environment is avoided, the good removing effect on zinc, chromium, copper and nickel with the high content in the soil is achieved, and the problems that in an existing heavy metal polluted soil repairing technology, the strains only have tolerance and leaching capability for single heavy metal, heavy metal tolerance is poor, reducing of toxicity of the heavy metal is small, the operation cost is high, and the environment condition requirements are harsh are solved.

Description

technical field [0001] The invention belongs to the technical field of microbial remediation of polluted environment, and relates to a method for removing heavy metals in different forms in polluted soil by acidophilic bacteria leaching. Background technique [0002] Soil is the material basis for human survival and the basic guarantee for social development. With the rapid development of science and information technology and rapid economic growth, the rapid upgrading of electronic products has produced a huge amount of electronic waste. E-waste is an untapped "secondary resource", known as "urban mine", which contains a large amount of precious metals and reusable devices. However, for a long time, my country's electronic dismantling and processing technology has been relatively backward, resulting in the release of a large amount of heavy metals and persistent organic pollutants into the environment, causing serious pollution to the local soil, water, and atmospheric env...

Claims

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Application Information

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IPC IPC(8): B09C1/10
CPCB09C1/10
Inventor 白建峰代双艳顾卫华苑文仪刘非凡杨敏徐琳琳庄绪宁赵静张承龙王景伟
Owner SHANGHAI SECOND POLYTECHNIC UNIVERSITY
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