85 results about "Microbial profiling" patented technology

The device for microbiological analyses on samples of body fluids comprising: an incubation area for containers containing said samples; an analyzer for analyzing the inner atmosphere of said containers; a sorting system to sort the containers according to the carbon dioxide content detected by said analyzer.

Methods and systems for identification of microorganisms either after isolation from a culture or directly from a sample. The methods and systems are configured to identify microorganisms based on the characterization of proteins of the microorganisms via high-resolution/mass accuracy single-stage (MS) or multi-stage (MSⁿ) mass spectrometry. Included herein are also discussion of targeted detection and evaluation of virulence factors, antibiotic resistance markers, antibiotic susceptibility markers, typing, or other characteristics using a method applicable to substantially all microorganisms and high-resolution/mass accuracy single-stage (MS) or multi-stage (MSⁿ) mass spectrometry.

The invention relates to a method for analyzing and detecting pathogenic microorganisms, which comprises the following steps: (1) filling immunological microspheres modified by antibodies into an immunological recognition/enrichment cavity; (2) introducing a sample solution to be measured into the immunological recognition/enrichment cavity to be recognized, captured and enriched by the immunological microspheres; (3) respectively transferring a cracking agent and a biological luminous reagent into the immunological recognition/enrichment cavity to carry out cracking reaction and biological luminous reaction with the enriched pathogenic microorganisms to generate a luminous composite; and (4) eluting the luminous composite generated in the step (3) with a buffer solution, and analyzing the pathogenic microorganisms in the sample to be measured through luminous detection. The method realizes separation/enrichment, inclusion cracking, biological luminous reaction and quantitative detection of the pathogenic microorganisms on the same micro-fluidic chip, not only reduces the consumption of the sample and the reagent but also avoids complicated operation, overlong analysis time and sample loss caused by a plurality of analysis steps or processes, and improves the analysis speed, the detection accuracy and the detection sensitivity.

The invention relates to the field of microbiological detection, in particular to a method and a device for microbiological analysis of a host sample. The method comprises the following steps: (1) performing first filtering processing on a sequencing data set from a host sample by adopting a host genome database so as to remove sequencing data which can be compared with the host genome database from the sequencing data set; (2) performing second filtering processing on the sequencing data set by adopting a homologous database so as to remove the sequencing data which can be compared with the homologous database from the sequencing data set; and (3) comparing the sequencing data set subjected to the first filtering treatment and the second filtering treatment with a microbial genome database so as to determine microbial sequencing data from the microorganisms in the sequencing data set. By applying the method and the device provided by the invention, rapid and accurate analysis and detection of microorganisms in a host can be realized.

The invention relates to a pathogenic microorganism analysis and identification system and application thereof, and belongs to the technical field of gene detection and analysis. The pathogenic microorganism analysis and identification system comprises: a data acquisition module used for acquiring gene sequencing data obtained by high-throughput sequencing; a data filtering module used for performing low-quality sequence filtering and host sequence filtering in sequence; a data comparison module used for comparing sequences to a pathogenic microorganism genome database; a species comparison module used for counting to-be-analyzed sequences; a data analysis module used for calculating the similarity S and the average similarity value SMSi of each species on each sequence comparison in the common comparison sequence set; a species sequence module used for calculating the total comparison sequence number SNTi of the species; and a result output module used for carrying out bioinformaticsanalysis to obtain a pathogenic microorganism analysis and identification result. The pathogenic microorganism analysis and identification system has the advantages of short analysis time and high accuracy, and can accurately detect mixed infection and obtain specific pathogen information.

The invention provides a microbial index evaluation method for the health condition of mangrove wetland sediment and belongs to the field of environmental monitoring. The method comprises the steps ofinvestigating the current situation of a mangrove wetland; determining indicator microorganisms; selecting ideal reference points; establishing a mangrove sediment sample collection method and determining microbial analysis methods, wherein the microbial analysis methods comprise a sample DNA extraction method, a PCR amplification method and a clone library construction method; selecting an indicator microorganism index evaluation factor and adopting a gene copy number of the indicator microorganisms, a diversity index and the proportion of dominant flora as evaluation factors; determining the weight Wi of the microorganism index evaluation factors; calculating an evaluation ratio Ii of the microbial index evaluation factors, and calculating an evaluation index P; determining the evaluation grade, and evaluating the health condition of the mangrove wetland sediment according to the evaluation grade. The provided microbial index evaluation method for the health condition of the mangrove wetland sediment can be used for accurately and effectively evaluating the health condition of the mangrove wetland sediment under field conditions and is wide in application range.

The microbe tracing method for oil deposit well includes the following steps: water quality analysis and autochthonous microbe analysis of the formation water; screening from available aerobic or facultative anaerobic microbe as tracing microbe, which has at least one of the three parameters of thallus form, colony form and growth temperature different from that of the autochthonous microbe of the aerobic or facultative anaerobic in the formation water and can maintain its life in the form of spore or other hypnozoite in the deposit environment; depositing the screened microbe into the deposit; sampling from corresponding oil producing well for detection; and performing analysis on deposit characteristic and deposited water flow characteristic based on the detection result. The said method is scientific and economic and has low cost, wide detection range, high sensitivity and no harm to human and environment.

The invention discloses a medical waste harmless treatment system based on network big data. The medical waste harmless treatment system comprises a hollow dustbin body, a bacterium standard exceedingalarm unit and a supervisory platform. The interior of the dustbin body is sequentially provided with two first partition plates in the horizontal direction, and the first partition plates sequentially divide the interior of the dustbin body into three cavities. The bacterium standard exceeding alarm unit comprises microorganism analysis meters and automatic alarm modules, all of which are arranged in the cavities. The supervisory platform comprises a dustbin information storage library recording related information of dustbins. The invention further discloses a medical waste harmless treatment system, wherein the medical waste harmless treatment system comprises a hollow dustbin body, an air purification bin arranged in a first cavity and separated from the first cavity through a secondpartition plate, a waste pulverizing bin arranged at the upper end in the second cavity, and an extrusion bin arranged at the upper end in a third cavity. The medical waste harmless treatment system can conduct bacterium number supervision on the dustbins in various departments of the hospital.

The invention belongs to the technical field of tertiary oil recovery, and particularly relates to an indigenous microorganism oil recovery method of a medium to high permeable oil reservoir. The indigenous microorganism oil recovery method specifically comprises the following steps that an oil reservoir is screened; indigenous microorganisms are analyzed; an activating agent is screened; a fieldinjection technology is optimized; and a field test is conducted and the effect is evaluated. The indigenous microorganism oil recovery method has the advantages that the technology is simple, the pertinence and the maneuverability are high, the mining cost is low, and subsequent treatment is not needed, and field application and promotion are facilitated; meanwhile, different injection technologies are adopted aiming at the difference of the oil layer penetration rates, the using amount of the injection agents can be effectively saved, meanwhile the use ratio of injected water is increased, the water driving wave and the volume are expanded, so that the development effect of water driving is improved, the field test enhanced oil recovery is larger than 15%, and the input-output ratio is larger than 1:5. Thus, the indigenous microorganism oil recovery method can be widely applied to the technical field of microorganism oil recovery.

A method of creating characteristic peak profiles of mass spectra and identification model for analyzing and identifying microorganisms are provided. MALDI-TOF MS data of microorganisms having the same feature are gathered. Discretization of the data is performed. Density-based clustering is used to find m/z values of spectral peaks with high probability of occurrence from the discretized data. A characteristic MS peak profile is created for every specific feature of microorganisms. Every such a characteristic profile forms a feature template. The mass spectrum of each known isolate is matched against all the feature templates and a number of matched vectors are obtained. The matched vectors are then concatenated into a single “integrated vector.” Then, a machine learning method and the integrated vectors generated from all known isolates are used to create a classification model for microorganism identification.

A gene chip for analyzing microbiocoenosis structure and function in acid environment is fast, sensitive and correct. The probe of the gene chip consists of acidophilic microbe 16S rRNA sequence and gene relating to carbon, nitrogen and iron metabolism, metal resistance and film, transposon and IST etc. functional gene sequence and minor human gene as negative contrast and quantitative contrast. It is constructed by 754-1072 oligomeric nucleotide sequences. It has high flux and less labor strength. It's easy to separate and culture acidophilic bacteria.

The invention relates to a filtration unit, comprising a membrane filter that can be disposed on a filter support of a bottom part and an attachment that can be placed on the bottom part, wherein the membrane filter has a reinforcing edge, and wherein the membrane filter can be clamped to a clamping part of a cover of a culture medium unit for removal purposes and introduced into the culture media unit. The invention further relates to a method for the microbiological analysis of liquid samples following a filtration process, wherein after removing an attachment a membrane filter is lifted off a filter support for pouring in the liquid sample and set down on a surface of a culture medium disposed in the bottom part of a culture medium unit, and the bottom part is covered by a cover. The cover is placed on the membrane resting on the filter support such that a clamping part present on the filter support clamps to a reinforcing edge of the membrane. The cover is lifted off the filter support together with the membrane and placed on the bowl-shaped bottom part such that the bottom of the membrane facing away from the cover rests on the top of the nutrient medium facing the cover.

The invention discloses a method for automatically surveying and mapping the growth curve of a microorganism in food. The method has the main contributions that a novel method that the microbiological analysis is carried out by surveying and mapping the growth curve of the microorganism is proposed starting with the growth rule of the microorganism in the food and is a technological innovation on the traditional analytical method; the condition that the growth information of the microorganism can be converted into measurable frequency information by a piezoelectric acoustic wave sensor is discovered through experimental study, and thus, a design principle for designing a fast and efficient novel surveying and mapping device is provided; and a device for automatically surveying and mapping the growth curve of the microorganism in the food is designed and constructed under the guidance of the novel method and the design principle. The device constructed according to the method has the advantages that the real-time and automatic surveying and mapping of the growth curve of the microorganism in the food can be realized, and the in-depth study on the microorganism in the food, such as the study of food processing methods, food shelf lives and microbial antimicrobial activity, can be carried out according to the obtained growth curve; and as the device has the characteristics of simplicity and convenience in operation, accuracy in result, and the like, the device is hopeful to be popularized and applied in microorganism-related research of food processing, storage and selling.

The invention discloses a microorganism analytical sampling device. The microorganism analytical sampling device comprises a cup-shaped body with the open upper end; an opening of the cup-shaped body is provided with a cover body; the cover body is matched with the opening of the cup-shaped body in size and shape; the bottom of the cup-shaped body is arranged into a funnel-shaped cup bottom; the middle of the funnel-shaped cup bottom is provided with a bottom end through hole; a bottom filter film is arranged below the bottom end through hole. According to the microorganism analytical sampling device, the structure is reasonably improved, the funnel-shaped cup bottom is matched with the bottom filter film particularly, and accordingly sputum can be conveniently sampled through the microorganism analytical sampling device without special air extraction device and accordingly the operation is convenient, the medical staff is well protected, and the significance on the sampling of dangerous bacteria such as tubercle bacilli is important.

The present invention provides an apparatus comprised of devices for simple, fast and cost-effective storage and plating of solid nutrient medium (SNM) for various uses in the field of microbiological analysis. The devices consist of a container which can be aseptically filled with SNM and equipped with a plunger to accurately press out needed amounts of the SNM; a cutting means to cut, hold and transfer SNM; a measuring means to measure the amount of SNM exiting the container; and a growth plate for storage of SNM, growth of microorganisms on SNM, and introduction of indicator substances to SNM for analysis of microorganisms.

The invention provides an erythrocyte aggregation detection method and a system thereof, which relate to the fields of biological and microbiological analysis detection. The method comprises the following steps: connecting an analysis module with an image information acquisition module and a control module for analyzing an obtained microscopic image; performing edge detection on erythrocyte in themicroscopic image, filling up an internal gas of the edge to form a complete erythrocyte shape image; performing a shrinkage analysis on an outer contour of the erythrocyte, and when the result is higher than a set value, determining the result as erythrocyte aggregation. calculating a ratio of an erythrocyte area with aggregation and an average area of the normal erythrocyte, and determining theerythrocyte aggregation degree according to the ratio. Compared with the prior art, the method employs a direct observation method and an image analysis method, the erythrocyte can be automatically identified, the area of an erythrocyte image and the area of the normal erythrocyte are compared, the erythrocyte aggregation degree is determined, the operation is convenient, the measurement efficiency is high, the identification precision is high, and the method can be used for quantitative analysis.

The invention discloses a rapid PM2.5 bacterial community composition source analysis and risk assessment method, and belongs to the technical field of microbial analysis. The rapid PM2.5 bacterial community composition source analysis and risk assessment method mainly comprises the following steps: sampling PM2.5 in an urban functional area, extracting microbial genomic DNA, performing PCR amplification on an extracted DNA product, purifying a PCR product, performing fluorescence quantification, performing high-throughput sequencing, performing bacterial diversity and abundance analysis, andperforming risk prediction. Compared with the conventional culture method, the rapid PM2.5 bacterial community composition source analysis and risk assessment method provided by the invention has theadvantages as follows: the bacterial community structure of a researched sample is reflected in a more detailed and comprehensive way, not only rapid and accurate analysis of the bacterial diversity of a particulate matter sample is achieved, but also the rapid PM2.5 bacterial community composition source analysis and risk assessment method is an effective method for evaluating the quality of theurban air environment and predicting disease risks. In summary, the rapid PM2.5 bacterial community composition source analysis and risk assessment method has the advantages of wide coverage, high detection accuracy and sensitivity, a more scientific and reliable prediction result and the like.