32 results about "Microbial profiling" patented technology

The utility model discloses a sample collector for analyzing harmful microorganisms in vegetables, which comprises a bottom plate, a sample box fixedly mounted at the top of the bottom plate, a fixed block fixedly mounted on the left side of the sample box, a vertical shaft fixedly mounted at the top of the fixed block, a connecting block rotatably mounted on the vertical shaft, and a box cover fixedly mounted on the connecting block. A discharging hole is formed in the box cover, a switching mechanism is arranged in the sample box, six sample tubes are movably mounted on the switching mechanism, and the switching mechanism is used for controlling the six sample tubes to alternate. The vegetable perforating and sampling device is reasonable in design, local areas with microorganisms on vegetables can be perforated and cut through the perforating pipe, samples possibly clamped in the perforating pipe are cleaned through the material returning rod when the perforating pipe ascends, next time of perforating and sampling can be conveniently carried out, the sampling efficiency is improved, the disc frame can be controlled to rotate by 60 degrees every time, and the sampling efficiency is improved. Furthermore, the sample tube is replaced, so that the vegetable sample is conveniently received, and the sampling efficiency is further improved.

The invention relates to the technical field of microbiological analysis, and particularly discloses an edible vegetable oil raw material fungus database construction method which comprises the following steps: collecting edible vegetable oil raw material data of various edible vegetable oils, and determining a plurality of raw material appearance structure clustering sets of each edible vegetable oil, a fungus separation method of each raw material appearance structure cluster set is provided; raw material fungus data and multiple raw material sample strain data of each edible vegetable oil are determined, and a raw material strain set of all edible vegetable oil is determined; determining strain characteristic data and raw material storage risk data of each edible vegetable oil; determining a plurality of types of sample data of each raw material sample strain data of each edible vegetable oil; and constructing a raw material database of each edible vegetable oil. The efficiency and the pertinence of fungus separation can be effectively improved; the accuracy and application value of the database are ensured, and efficient data support is provided for related research and industrial application of edible vegetable oil raw material fungi.

The invention discloses a method for analyzing microbial diversity and organic acid difference of yellow water of different years, which comprises the following steps: S1, carrying out sample standardization treatment: taking a yellow water sample, adding a DNA (Deoxyribonucleic Acid) protective agent into one part of the sample as a microbial analysis sample, and storing at low temperature; the other part of the sample is filtered by an organic phase filter membrane to be used as an organic acid analysis sample and stored at low temperature; s2, microbial community analysis: performing DNA extraction, high-throughput sequencing and bioinformatics analysis on a microbial analysis sample to screen differential bacteria; s3, organic acid difference detection: acidifying the organic acid analysis sample, purifying the eluent by using an extraction column, and carrying out vacuum concentration and constant volume; analyzing and evaluating differences among groups, and screening different organic acids; and S4, multi-omics correlation verification: constructing an abundance-content matrix of the differential bacteria and the differential organic acids, calculating a Pearson correlation coefficient to determine significant correlation, annotating a metabolic pathway of the differential bacteria, and establishing a correlation model of the differential bacteria, the characteristic organic acids and the metabolic pathway.

The invention discloses a constructed wetland pollution reduction and carbon reduction synergistic effect analysis system and method, and relates to the technical field of water pollution control and greenhouse gas emission reduction. Comprising a simulated wetland reaction unit, a multi-parameter monitoring module, an operation regulation and control module, a microbiological analysis module and a synergistic effect evaluation module. The simulated wetland reaction unit is filled with a FeC composite matrix to realize subsurface flow constructed wetland environment simulation; the multi-parameter monitoring module acquires water quality and greenhouse gas emission data in real time; the operation regulation and control module realizes accurate regulation and control of water inlet parameters; the microbiological analysis module analyzes microflora and functional gene information; and the synergistic effect evaluation module integrates multi-module data to construct a correlation model, calculates a synergistic effect index and outputs an optimization parameter. Through five stages of matrix optimization, operation regulation and control, microbial mechanism revealing, collaborative evaluation optimization and feedback application, evaluation and optimization of the constructed wetland pollution reduction and carbon reduction collaborative efficiency are realized, and a scientific basis is provided for actual constructed wetland engineering design and operation.

The utility model discloses soil collection device convenient to microorganism analysis, include: fixed platform, the bottom of fixed platform is installed with fixed support leg, movable platform, movable platform sliding connection is in fixed platform, and the telescopic support leg is installed on movable platform, plane movement subassembly, plane movement subassembly includes fixed seat, X -axis movement subassembly and Y -axis movement subassembly, and X -axis movement subassembly and Y -axis movement subassembly are arranged in perpendicular state between fixed seat and fixed on movable platform top surface, and X -axis movement subassembly and Y -axis movement subassembly are transmission cooperation between fixed seat and fixed platform, are used for controlling movable platform and move on plane, multi -freedom degree mechanical arm, two groups are provided to multi -freedom degree mechanical arm, and two groups multi -freedom degree mechanical arm symmetrical installation are in movable platform bottom both sides, take out soil subassembly, take out soil subassembly includes power component and take out soil bucket, and take out soil bucket detachable connection is in power component's output end. The whole sampling process in the application, and mechanical operation reduces the damage to the original structure of soil.

The invention discloses a differential diagnosis and prognosis evaluation method for a pleural effusion microbiome based on metagenome sequencing and application, belongs to the technical field of bioinformatics, and discloses a method for analyzing the microbiome of a pleural effusion sample, and the method comprises the following steps: carrying out metagenome next-generation sequencing on an in-vitro pleural effusion sample to obtain a sequencing reading; performing bioinformatics analysis on the sequencing reading, and determining microorganism composition information of the pleural effusion sample based on a verification result; by establishing a unified mNGS experimental process and a parallel bioinformatics analysis path, two kinds of technical information of different dimensions, namely microbiome structural characteristics and host genome copy number variation (CNV), are synchronously output from single sequencing data, and the defect that microbiological analysis and genome analysis need to be carried out in batches in the prior art is overcome.

The present disclosure relates to the field of microbiological analysis using a miniaturized cellulose-based microbiological culture device and a method to produce the same. Particularly to a device designed to detect microorganisms in various samples using a specially configured cellulose substrate.

A grade of a specific microbial disease in a biological sample of a subject is determined. In one example, masked microbial reference genomes are used to determine the amount of free DNA molecules corresponding to a particular microbial species associated with a particular microbial disease. The masking may remove an area shared with another. In another example technique, end motifs are used for free DNA fragments from a subject and from a particular microbial species. A correlation between a subject and an amount of a set of terminal sequence motifs of a particular microbial species may be determined. The two groups of quantities of positive subjects are substantially more correlated than negative subjects.

The application provides a kind of microbial detection analysis device, it is related to the field of microbial detection analysis, comprising: container assembly, the container assembly includes medicament storage and piston mechanism, control component can be linked piston mechanism to realize the operation of liquid injection to the inside of culture dish, under the control of control component, the fixed injection amount of piston mechanism each time, can quickly prepare a large number of same samples for analysis and detection use, greatly improve the accuracy of microbial detection analysis result, at the same time, the amount of single injection of piston mechanism can also be regulated, can adapt to different microbial analysis and detection sample preparation operation etc., solve the problem that multiple samples are prepared in the existing microbial analysis and detection, corresponding liquid medicine needs to be injected into the inside of culture dish by liquid injector manually, liquid injector does not have the function of quantitative liquid injection, the amount of liquid medicine in the inside of culture dish is not the same, easy to interfere with the result of microbial detection analysis, the problem of poor accuracy.

This invention relates to a method for detecting pathogenic bacteria based on magnetic coding and a microfluidic separation system, belonging to the field of microbial analysis and detection technology. The core of the magnetic coding-based spatial separation system of this invention includes a single-channel microfluidic chip, a 3D-printed magnetic separation module, and a fluorescence-UV dual-mode detection light source and a fluorescence-UV dual-mode probe. The microfluidic device of this invention can precisely control the fluid dynamics and magnetic field gradient distribution within the channel, thereby efficiently manipulating and separating magnetic nanospheres, further enhancing the accuracy of multi-target analysis. Simultaneously, the directional enrichment of magnetically labeled particles within the microchannel can directly eliminate the lengthy sample concentration step, significantly shortening the total analysis time. Combined with optical or electrochemical sensors, it can achieve rapid real-time detection of target molecules.

The utility model discloses a water source detection sampling instrument for engineering geological investigation, and belongs to the technical field of water source detection sampling. A water source detection sampling instrument for engineering geological investigation comprises a fixing base, a fixing column, a winch, a sampling vessel and a water source filtering mechanism, and the fixing column is fixedly connected to the bottom of the fixing base; the winch is connected and fixedly arranged on the fixed seat; the sampling vessel is connected and fixedly arranged on the winch; by arranging the water source filtering mechanism, suspended solids or sediments in a water sample can be automatically removed, so that the water sample is purer, interference to subsequent analysis is reduced, and when heavy metal analysis, microbiological analysis or other detailed chemical analysis needs to be carried out, the water source filtering mechanism can be used for filtering the water sample. The filtering function can help to remove impurities in the water sample, so that the data accuracy is ensured, and the practicability of the device is improved.

A method for analyzing a microorganism including an identification step for determining which of abony and pakistan which are two serotypes of Salmonella bacteria is contained in a sample which contains either abony or pakistan, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of minnesota, infantis and brandenburg which are three serotypes of Salmonella bacteria is contained in a sample which contains minnesota, infantis or brandenburg, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample, or a method for analyzing a microorganism including an identification step for determining which of schwarzengrund and montevideo which are two serotypes of Salmonella bacteria is contained in a sample which contains either schwarzengrund or montevideo, based on the presence or absence of a peak (or peaks) at a predetermined mass-to-charge ratio (or ratios) in a mass spectrum obtained by a mass spectrometric analysis of the sample.

This invention belongs to the field of pathogen detection technology and discloses a method for pathogen analysis based on long-read sequencing data. Specifically, it includes: establishing an error-correcting reference sequence library; aligning the quality-controlled sequencing data to the error-correcting reference sequence library for correction; merging sequences with high similarity after correction; and then aligning them to a pathogen identification database to obtain a list of detected microorganisms. This invention effectively improves data analysis speed and reduces false positives from long-read sequencing data by correcting and merging sequencing data, achieving rapid and accurate detection of pathogens.

The application belongs to the technical field of pathogenic microorganism detection, and discloses a multi-target pathogenic microorganism analysis method based on third-generation targeted sequencing data, specifically: a multi-target correlation error correction reference sequence library is established, original sequencing data is quality controlled, then is compared to a pathogenic microorganism identification database to obtain a preliminary microorganism detection list, a pathogenic microorganism detection library is constructed based on the list and the error correction reference sequence library, and the quality controlled sequencing data is compared to the pathogenic microorganism detection library to obtain an accurate microorganism detection list. The method can effectively solve the accuracy problem of single-target identification, realize accurate detection of the species composition in the sample to be detected, and improve the accuracy and sensitivity of data analysis.

A concentration, extraction and identification system (CEIS) for microbiological analysis of a biological sample, includes: (1) a plurality of paramagnetic particles adapted to bind to a nucleic acid fraction of a targeted biological pathogen, (2) a microfluidic chip including a plurality of interconnected wells; and (3) a microfluidic chip reading instrument including a housing carrying (a) a microfluidic chip receiver adapted to hold the microfluidic chip and (b) a magnet and actuator adapted to displace the plurality of paramagnetic particles from a biological sample well through a first isolation buffer well, a wash well, and a second isolation buffer well to an elution well.

The present invention relates to the technical field of microbiological analysis, and discloses a single cell distributor, which comprises a platform, a three-axis motion mechanical arm arranged on the platform, and a control system arranged in the platform, the three-axis motion mechanical arm is arranged on one side of the platform, and the other side of the platform is provided with a mounting seat for placing a pore plate, one end or two ends of the mounting seat are provided with placing stations for placing liquid storage bottles, the three-axis movement mechanical arm is connected with a distribution assembly, the distribution pressure of the distribution assembly is less than or equal to 0.1 psi, the distribution assembly comprises a seat body, and the lower end of the seat body is provided with a sensor suction head and a spray head; a pressure sensor and a Coulter counter which are communicated with the sensor suction head and the spray head are arranged in the base body, and the pressure sensor, the Coulter counter and the three-axis movement mechanical arm are all electrically connected with the control system. According to the method, rapid, efficient and low-cost single cell high-throughput analysis and separation can be realized, meanwhile, the simplicity and convenience of operation and high survival rate of cells are ensured, and the data processing and analysis capability is expanded.

The invention provides a soil microorganism identification and analysis method and system based on a decision tree, and the method comprises the steps: determining a production reduction influence coefficient caused by wrong classification of pathogenic bacteria by the decision tree through obtaining the associated data of soil pathogenic bacteria in a soil sample and a microenvironment; according to the output reduction influence coefficient, combining with different misclassification types of pathogenic bacteria, constructing a cost sensitive matrix during soil pathogenic bacteria identification; determining a correlation coefficient between the soil microenvironment parameters and the abundance of pathogenic bacteria, and extracting a reference interval according to the correlation coefficient; determining information gains of all soil samples in different branches of a decision tree according to a reference interval, and generating an initial decision tree for soil pathogen identification according to the cost-sensitive matrix; determining the disease risk cost of each branch, determining a post-pruning strategy of the initial decision tree, and further generating a soil microorganism analysis report of the to-be-detected sample. By adopting the scheme of the invention, hierarchical optimization can be carried out on the decision tree to avoid the risk of missed judgment of high-hazard bacteria.

The present invention provides systems and methods for the detection of systemic disease from bacterial content samples obtained from a subject. Also provided are methods of training a machine learning algorithm to correlate patient microbiome sequence data with a disease state or disease development risk. These systems and methods utilize a high-resolution, database-independent, high-throughput microbial profiling platform to diagnose systemic disease in patients or to identify those patients at risk of developing systemic disease. Also provided are systems and kits for carrying out the methods.

The present invention provides systems and methods for the detection of systemic disease from bacterial content samples, such as for example gut microbiome samples, obtained from a subject. Also provided are methods of training a machine learning algorithm to correlate patient microbiome sequence data with a disease state or disease development risk. These systems and methods utilize a high-resolution, database-independent, high-throughput microbial profiling platform to diagnose systemic disease in patients or to identify those patients at risk of developing systemic disease. Also provided are systems and kits for carrying out the methods.

The invention relates to a method for detecting multiple pathogenic bacteria by using magnetic separation time difference, and belongs to the technical field of microbiological analysis and detection. According to the invention, the dual-mode aptamer sensor is integrated, so that rapid and accurate multiple pathogenic bacteria detection can be realized. The core of the time separation system based on magnetic coding comprises fluorescent magnetic coding nanoparticles, a gold nanoparticle-aptamer and a fluorescent-ultraviolet dual-mode probe. A preparation process of the fluorescent-ultraviolet dual-mode probe specifically comprises the following steps: sequentially anchoring quantum dots and specific cDNA on the surface of a magnetic coding nanosphere; and carrying out functional combination with aptamer-modified gold nanoparticles to finally synthesize the dual-mode probe with both fluorescence signal response and ultraviolet absorption response. The probe can realize targeted recognition and separation detection of various pathogenic bacteria through specific binding of the surface aptamer and target pathogenic bacteria.

The invention relates to a leakproof double-insurance chloroform fumigation device for microbial biomass carbon, nitrogen and phosphorus analysis, and belongs to the technical field of microbiological analysis experiment equipment.The leakproof double-insurance chloroform fumigation device comprises a fumigation box and a sealing cover, an annular inserting groove is formed in the bottom face of the sealing cover, and a first sealing gasket is embedded in the annular inserting groove; a lifting assembly used for driving the sealing cover to vertically ascend and descend is arranged outside the fumigation box. An annular frame extending horizontally is fixedly arranged on the inner wall of the fumigation box, the annular frame and the fumigation box are integrally formed, and a second sealing gasket is fixedly arranged on the lower surface of the annular frame; a turnover sealing plate is arranged below the annular frame body, and the edge of one side of the sealing plate is hinged to the inner wall of the fumigation box; an overturning assembly used for driving the sealing plate to rotate is further arranged in the fumigation box; a placement frame for bearing samples is horizontally arranged in the fumigation box, a moving assembly for driving the placement frame to move in the vertical direction is further arranged in the fumigation box, and the device has the effect of improving the detection accuracy of carbon, nitrogen and phosphorus in the microbial biomass.

The present application relates to the field of soil microbial analysis, in particular to a method for analyzing the rhizosphere microbial community structure of cultivated Fritillaria delavayi Hsiao and application thereof. The method comprises the following steps: step one, sample collection and processing; step two, determination of soil physicochemical properties; step three, gene sequencing: 16S amplification sequencing and high-throughput sequencing; step four, data analysis: conducting microbial community diversity analysis, community composition analysis, differential group analysis, community structure difference analysis, and correlation analysis of soil physicochemical properties and microbial community. The method can determine the dominant bacterial groups in the rhizosphere soil of Fritillaria delavayi Hsiao and the regulation factors of the dominant bacterial groups by physicochemical properties, provide a theoretical basis for the standardized planting of Fritillaria delavayi Hsiao and the screening of geographical provenances with excellent microbial interaction ability, and improve the yield and medicinal ingredients of cultivated Fritillaria delavayi Hsiao.

The application discloses a kit for rapidly detecting bacterial energy metabolism and a preparation method and application thereof, and belongs to the technical field of microorganism analysis, drug screening and detection. The kit comprises reaction premixed powder, anaerobic reaction liquid and optional positive control; the reaction premixed powder comprises the following components in parts by weight: 1 part of benzyl viologen; 0.43-0.64 parts of sodium pyruvate; 0.054-0.082 parts of coenzyme A; 0.037-0.055 parts of MgCl2.7H2O; the anaerobic reaction liquid is a buffer solution containing sodium sulfide-9 hydrate; and the positive control is nitazoxanide or glychalcone A. The application further discloses a preparation method and application of the kit, and the application comprises the following steps: rapidly detecting bacterial energy metabolism activity; and / or screening substances for inhibiting bacterial energy metabolism. The kit can rapidly, sensitively and at low cost evaluate the physiological activity of bacteria, and can also be used for rapidly screening and evaluating anaerobic microorganism energy metabolism inhibitors.

The present invention includes: a step (S0) for acquiring, for each of a first bacterial group and a second bacterial group belonging to the order Enterobacterales and being different from each other in at least one classification below the family level, a plurality of full-length sequences which are full-length amino acid sequences of a plurality of corresponding acid shock proteins; a step (S1) for identifying a cleavage sequence indicating a site in which the plurality of full-length sequences are fragmented; and steps (S2)-(S8) for obtaining a mass spectral pattern of a plurality of acid shock protein fragments obtained by fragmenting each of the plurality of full-length sequences, and calculating the identification ratios of the first bacterial group and the second bacterial group on the basis of the mass spectral pattern.

A microbiological analysis system (1) estimates whether or not a target microbe (212) is present in a target space (SP), or estimates the amount of the target microbe (212) present in the target space (SP). The microbiological analysis system (1) is provided with a first information acquisition unit (20) and a control unit (40). A first information acquisition unit (20) acquires first information (21) relating to a subject space (SP), said first information being information that differs from the presence or absence of a subject microorganism (212) itself and the amount of the subject microorganism (212) present. The control unit (40) estimates, on the basis of the first information (21), the presence or absence of the target microorganism (212) in the target space (SP) or the amount of the target microorganism (212).

The present disclosure relates to the field of microbiological analysis using a miniaturized cellulose-based microbiological culture device and a method to produce the same. Particularly to a device designed to detect microorganisms in various samples using a specially configured cellulose substrate.

Marine disease microorganisms are one of important factors influencing national economic construction, and development of disease microorganism detection and control research has great significance on national economic construction. The quick microorganism detection system based on the lysozyme labeled bacteriophage is researched and developed, the key point of the quick microorganism detection system is that a lysozyme labeled engineered bacteriophage kit is used for directly analyzing microorganisms in an environmental biological sample, and the lysozyme labeled engineered bacteriophage can specifically recognize pathogenic microorganisms. After the engineered bacteriophage recognizes and is combined with the microorganism, the genetic engineering modified DNA of the engineered bacteriophage is injected into the microorganism, and a large number of new bacteriophages with lysozyme protein are recombined, expressed and amplified in the microorganism; the main content of the project is as follows: a recognition mechanism and reaction kinetics of the engineered bacteriophage and a microbial response material are designed emphatically, and the action rule of functional modules of recognition response in microbial rapid detection and microbial instant expression analysis is investigated; the innovation of the work is reflected in that the action relationship between the functionalized bacteriophage and the microorganisms is disclosed on the basic research level, the action rule of a one-stop microorganism analyzer and different microorganisms is summarized, and reference is particularly provided for solving the online, portable and full-automatic electromechanical engineering and biology compounding problem in a microorganism detection method.

The invention relates to the technical field of microbiological analysis and detection, in particular to a rapid detection method for flora characteristics. The detection method disclosed by the invention is a method for rapidly detecting flora characteristics of a target sample based on rapid bacterial lysis and a high-throughput sequencing technology, bacteria can be fully lysed, preparation of a 16S library can be rapidly completed, and then the bacteria are accurately quantified through high-throughput sequencing; the copy number of the bacteria is corrected by adopting the 16S rDNA content of the total bacteria in the sample to be detected, so that the difference between the reads number of the bacteria and the copy number result caused by different sampling amounts can be eliminated, the sampling deviation is eliminated, the detection result is more accurate, the rapid detection of the bacterial flora characteristics in the target sample is realized, and the detection efficiency is improved. The method has a wide application prospect in the field of bacterial diversity analysis.

The invention discloses a Pinus densiflora rhizosphere soil microbial diversity analysis system and method in the technical field of soil microbial analysis, and the system comprises a rhizosphere positioning unit which is used for determining a target Pinus densiflora and marking coordinates of a tricholoma matsutake active region and a healthy control region in a rhizosphere core region of the target Pinus densiflora; the sample collection unit is used for collecting soil samples in the marked tricholoma matsutake active region and the marked healthy control region; the processing unit is used for processing the soil sample to obtain DNA data of the soil sample; and the analysis unit is used for carrying out soil microorganism identification on the DNA data of the soil sample and judging the microbial diversity of the soil sample according to an identification result. The rhizosphere positioning unit can quickly position target Pinus densiflora and mark coordinates of a tricholoma matsutake active area and a healthy control area in a rhizosphere core area of the Pinus densiflora, accurate space guidance is provided for manual soil sample collection, and the problems of large positioning error and insufficient sample representativeness caused by subjective experience judgment in traditional manual sampling are effectively solved.

The utility model provides a device for monitoring and evaluating the microbial effect of drug combination on dairy products, and relates to the technical field of microbial analysis. The main body of the device for monitoring and evaluating the microbial effect of the drug combination on the dairy product is a microbial effect migration disc. The microbial effect migration disc comprises a round disc body formed by a bottom plate and side plates. The separation ring table is arranged on the bottom plate in a protruding mode so as to divide the inner space of the tray body into an inner ring space and an outer ring space. The inner ring space is mainly used for inoculating primary microorganisms; the outer ring space is provided with a plurality of partition plates, and the partition plates are arranged in the outer ring space in the circumferential direction of the outer ring space at intervals so as to divide the outer ring space into a plurality of independent isolation spaces. The utility model aims at synchronously and in-situ testing the microbial effect in the dairy product by using different combinations, different proportions and different doses of drug combination in the same microbial effect migration plate, so that high-throughput, visual and in-situ control is realized, and errors caused by inconsistency of microbial flora sources are effectively avoided.