86 results about "Microbial profiling" patented technology

The device for microbiological analyses on samples of body fluids comprising: an incubation area for containers containing said samples; an analyzer for analyzing the inner atmosphere of said containers; a sorting system to sort the containers according to the carbon dioxide content detected by said analyzer.

The present invention refers to manual devices for the collecting and concentrating liquid samples for microbiological analysis and their respective methods of use. The manual devices comprise a body which contains a sample, a removable support, a microporous membrane, and a plunger. The present invention is also directed to methods of collecting and concentrating of liquid samples onto a microporous membrane for microbiological analysis.

To reduce the effects of the contaminants in the measurement of microorganisms and the reduction of the time necessary for the measurement. Measurement is performed of the microorganism prior to and following culture, and the difference between the two is found. This prevents errors caused by the effect of contaminants contained in the specimens. Since the measurement of the microorganism is performed by means of a flow cytometer, the microorganisms can be measured even when the culture period is short. Moreover, the measurements are accurate, since the contaminants are not measured. Furthermore, the growth form of the microorganisms can be determined by measuring the changes in the intensity of the light emission over the duration of emission of the forward scattered light detected by means of a flow cytometer. Accordingly, based on differences in the particle-size distribution prior to and following culture, it is possible to formulate five major bacterial classifications: Bacilli, Staphylococci, Streptobacilli, Streptococci and yeast fungi.

Methods and systems for identification of microorganisms either after isolation from a culture or directly from a sample. The methods and systems are configured to identify microorganisms based on the characterization of proteins of the microorganisms via high-resolution / mass accuracy single-stage (MS) or multi-stage (MSn) mass spectrometry. Included herein are also discussion of targeted detection and evaluation of virulence factors, antibiotic resistance markers, antibiotic susceptibility markers, typing, or other characteristics using a method applicable to substantially all microorganisms and high-resolution / mass accuracy single-stage (MS) or multi-stage (MSn) mass spectrometry.

A microbiological analyzer for performing ID tests on samples using colorimetric techniques to generate a pixel-wise colored map of a test region of interest and also performing MIC tests on samples using nephelometric techniques to determine which antimicrobial agents are most effective against a particular microorganism.

The present invention refers to articles for collecting samples from a surface, articles for microbiological analyses of said samples, and methods of use of said articles. The articles include sample collectors, sample housings with optional barrier layers, and sample-ready reagent strips comprising hydrophilic agents to grow and detect microorganisms. The disclosure includes methods to collect, detect, and quantify microorganisms in a surface sample.

The invention relates to a method for analyzing and detecting pathogenic microorganisms, which comprises the following steps: (1) filling immunological microspheres modified by antibodies into an immunological recognition / enrichment cavity; (2) introducing a sample solution to be measured into the immunological recognition / enrichment cavity to be recognized, captured and enriched by the immunological microspheres; (3) respectively transferring a cracking agent and a biological luminous reagent into the immunological recognition / enrichment cavity to carry out cracking reaction and biological luminous reaction with the enriched pathogenic microorganisms to generate a luminous composite; and (4) eluting the luminous composite generated in the step (3) with a buffer solution, and analyzing the pathogenic microorganisms in the sample to be measured through luminous detection. The method realizes separation / enrichment, inclusion cracking, biological luminous reaction and quantitative detection of the pathogenic microorganisms on the same micro-fluidic chip, not only reduces the consumption of the sample and the reagent but also avoids complicated operation, overlong analysis time and sample loss caused by a plurality of analysis steps or processes, and improves the analysis speed, the detection accuracy and the detection sensitivity.

The invention relates to a nutrient medium unit for holding a filter of a filtration device, comprising a lower part that is filled with the nutrient medium and a lid, the latter having a fixing edge that protrudes into the lower part and that can be connected to an edge of the filter by means of an adhesive bond in order to remove said filter from the filtration device. The invention also relates to a method for the microbiological analysis of liquid samples, according to which a membrane filter is lifted off a filter support and laid on the surface of a nutrient medium that is situated in a lower part of a nutrient medium unit. The lower part is then covered by a lid, the latter being placed on the membrane filter lying on the filter support in such a way that a fixing edge located in the lid is connected to an edge of the filter by means of an adhesive bond. The lid and the attached filter are lifted off the filter support and placed on the dish-shaped lower part of the nutrient medium unit.

The invention relates to the field of microbiological detection, in particular to a method and a device for microbiological analysis of a host sample. The method comprises the following steps: (1) performing first filtering processing on a sequencing data set from a host sample by adopting a host genome database so as to remove sequencing data which can be compared with the host genome database from the sequencing data set; (2) performing second filtering processing on the sequencing data set by adopting a homologous database so as to remove the sequencing data which can be compared with the homologous database from the sequencing data set; and (3) comparing the sequencing data set subjected to the first filtering treatment and the second filtering treatment with a microbial genome database so as to determine microbial sequencing data from the microorganisms in the sequencing data set. By applying the method and the device provided by the invention, rapid and accurate analysis and detection of microorganisms in a host can be realized.

The invention relates to a pathogenic microorganism analysis and identification system and application thereof, and belongs to the technical field of gene detection and analysis. The pathogenic microorganism analysis and identification system comprises: a data acquisition module used for acquiring gene sequencing data obtained by high-throughput sequencing; a data filtering module used for performing low-quality sequence filtering and host sequence filtering in sequence; a data comparison module used for comparing sequences to a pathogenic microorganism genome database; a species comparison module used for counting to-be-analyzed sequences; a data analysis module used for calculating the similarity S and the average similarity value SMSi of each species on each sequence comparison in the common comparison sequence set; a species sequence module used for calculating the total comparison sequence number SNTi of the species; and a result output module used for carrying out bioinformaticsanalysis to obtain a pathogenic microorganism analysis and identification result. The pathogenic microorganism analysis and identification system has the advantages of short analysis time and high accuracy, and can accurately detect mixed infection and obtain specific pathogen information.

The invention provides a microbial index evaluation method for the health condition of mangrove wetland sediment and belongs to the field of environmental monitoring. The method comprises the steps ofinvestigating the current situation of a mangrove wetland; determining indicator microorganisms; selecting ideal reference points; establishing a mangrove sediment sample collection method and determining microbial analysis methods, wherein the microbial analysis methods comprise a sample DNA extraction method, a PCR amplification method and a clone library construction method; selecting an indicator microorganism index evaluation factor and adopting a gene copy number of the indicator microorganisms, a diversity index and the proportion of dominant flora as evaluation factors; determining the weight Wi of the microorganism index evaluation factors; calculating an evaluation ratio Ii of the microbial index evaluation factors, and calculating an evaluation index P; determining the evaluation grade, and evaluating the health condition of the mangrove wetland sediment according to the evaluation grade. The provided microbial index evaluation method for the health condition of the mangrove wetland sediment can be used for accurately and effectively evaluating the health condition of the mangrove wetland sediment under field conditions and is wide in application range.

The microbe tracing method for oil deposit well includes the following steps: water quality analysis and autochthonous microbe analysis of the formation water; screening from available aerobic or facultative anaerobic microbe as tracing microbe, which has at least one of the three parameters of thallus form, colony form and growth temperature different from that of the autochthonous microbe of the aerobic or facultative anaerobic in the formation water and can maintain its life in the form of spore or other hypnozoite in the deposit environment; depositing the screened microbe into the deposit; sampling from corresponding oil producing well for detection; and performing analysis on deposit characteristic and deposited water flow characteristic based on the detection result. The said method is scientific and economic and has low cost, wide detection range, high sensitivity and no harm to human and environment.

The present invention provides for a method for selecting an antimicrobial agent, which comprises a microbe analyzing step in which microbes in the microbial biota of a sample are analyzed based on base sequence of DNA and an antimicrobial agent selecting step comprising performing search in a data base storing data for industrial antimicrobial agents applicable to the analyzed microbes recited at effective concentrations, picking up industrial antimicrobial agents effective for the microbial biota, dominant microbe or specific microbe(s) in the sample analyzed in the above microbe analyzing step and selecting among the picked up ones an industrial antimicrobial agent, which method permits to select an antimicrobial agent that is optimum for the treatment in a simple and assured manner within a brief time and to grasp whether the effect of the antimicrobial agent is revealed or not in a simple and assured manner within a brief time, by utilizing this selecting method for the selection of antimicrobial agent, for example, in an antimicrobial treating, in monitoring of the antimicrobial effect, in slime control in paper manufacturing process courses and in microbe inhibition in paper manufacturing process courses, whereby troubles caused by growth of microbes can be prevented beforehand.

The present invention is concerned with a method of identifying microbial strains (e.g. from a cell culture), the method comprising; i) a lipid extraction step, comprising extraction of phospholipids from the microbe, suitably with an extraction composition comprising more than 50 vol % MeOH; ii) a sample preparation step, comprising preparation of a MALDI sample incorporating the extracted lipids; iii) a data gathering step, comprising performing MALDI-based mass spectrometry on the MALDI sample, and iv) a microbe identification step, comprising analysis of the mass spectrometry data to characterise or identify the microbial strain. Suitably the method also includes extracting proteins from the microbes and analysing the extracted proteins using MALDI-based mass spectrometry so as to obtain not only lipid m / z data but also protein m / z data.

The invention discloses a medical waste harmless treatment system based on network big data. The medical waste harmless treatment system comprises a hollow dustbin body, a bacterium standard exceedingalarm unit and a supervisory platform. The interior of the dustbin body is sequentially provided with two first partition plates in the horizontal direction, and the first partition plates sequentially divide the interior of the dustbin body into three cavities. The bacterium standard exceeding alarm unit comprises microorganism analysis meters and automatic alarm modules, all of which are arranged in the cavities. The supervisory platform comprises a dustbin information storage library recording related information of dustbins. The invention further discloses a medical waste harmless treatment system, wherein the medical waste harmless treatment system comprises a hollow dustbin body, an air purification bin arranged in a first cavity and separated from the first cavity through a secondpartition plate, a waste pulverizing bin arranged at the upper end in the second cavity, and an extrusion bin arranged at the upper end in a third cavity. The medical waste harmless treatment system can conduct bacterium number supervision on the dustbins in various departments of the hospital.

The invention provides a cervical carcinoma and uterus cancer detection method and a system thereof, which relate to the fields of biological and microbiological analysis detection. The method comprises the following steps: obtaining a microscopic image of vaginal secretion; performing edge detection, filling up an internal gas of the edge to form a complete exfoliative cell shape; obtaining a microscopic image of exfoliative cell, performing edge detection to obtain cell nucleus; calculating an area difference and curvature of the exfoliative cell to determine the result as cervical carcinomaand uterus cancer; calculating the ovality of the exfoliative cell, and when the ovality of the exfoliative cell is lower than a first set value, determining the result as precancerous lesions of cervical carcinoma and uterus cancer; calculating a ratio of an area of the exfoliative cell nucleus to the area of the exfoliative cell, and when the ratio of the area of the exfoliative cell nucleus tothe area of the exfoliative cell is higher than a second set value, determining the result as an early stage of precancerous lesions of cervical carcinoma and uterus cancer. Compared with the prior art, the method has the advantages that the measurement time is short, the complex processes such as dyeing culture cannot be required, and the process is simple and reliable.

The invention belongs to the technical field of tertiary oil recovery, and particularly relates to an indigenous microorganism oil recovery method of a medium to high permeable oil reservoir. The indigenous microorganism oil recovery method specifically comprises the following steps that an oil reservoir is screened; indigenous microorganisms are analyzed; an activating agent is screened; a fieldinjection technology is optimized; and a field test is conducted and the effect is evaluated. The indigenous microorganism oil recovery method has the advantages that the technology is simple, the pertinence and the maneuverability are high, the mining cost is low, and subsequent treatment is not needed, and field application and promotion are facilitated; meanwhile, different injection technologies are adopted aiming at the difference of the oil layer penetration rates, the using amount of the injection agents can be effectively saved, meanwhile the use ratio of injected water is increased, the water driving wave and the volume are expanded, so that the development effect of water driving is improved, the field test enhanced oil recovery is larger than 15%, and the input-output ratio is larger than 1:5. Thus, the indigenous microorganism oil recovery method can be widely applied to the technical field of microorganism oil recovery.

A method of creating characteristic peak profiles of mass spectra and identification model for analyzing and identifying microorganisms are provided. MALDI-TOF MS data of microorganisms having the same feature are gathered. Discretization of the data is performed. Density-based clustering is used to find m / z values of spectral peaks with high probability of occurrence from the discretized data. A characteristic MS peak profile is created for every specific feature of microorganisms. Every such a characteristic profile forms a feature template. The mass spectrum of each known isolate is matched against all the feature templates and a number of matched vectors are obtained. The matched vectors are then concatenated into a single “integrated vector.” Then, a machine learning method and the integrated vectors generated from all known isolates are used to create a classification model for microorganism identification.

The invention relates to microbial analysis. The present invention is concerned with a method of identifying microbial strains (e.g. from a cell culture), the method comprising; i) a lipid extraction step, comprising extraction of phospholipids from the microbe, suitably with an extraction composition comprising more than 50vol% MeOH; ii) a sample preparation step, comprising preparation of a MALDI sample incorporating the extracted lipids; iii) a data gathering step, comprising performing MALDI-based mass spectrometry on the MALDI sample, and iv) a microbe identification step, comprising analysis of the mass spectrometry data to characterise or identify the microbial strain. Suitably the method also includes extracting proteins from the microbes and analysing the extracted proteins using MALDI-based mass spectrometry so as to obtain not only lipid m / z data but also protein m / z data.

A gene chip for analyzing microbiocoenosis structure and function in acid environment is fast, sensitive and correct. The probe of the gene chip consists of acidophilic microbe 16S rRNA sequence and gene relating to carbon, nitrogen and iron metabolism, metal resistance and film, transposon and IST etc. functional gene sequence and minor human gene as negative contrast and quantitative contrast. It is constructed by 754-1072 oligomeric nucleotide sequences. It has high flux and less labor strength. It's easy to separate and culture acidophilic bacteria.

The invention relates to a filtration unit, comprising a membrane filter that can be disposed on a filter support of a bottom part and an attachment that can be placed on the bottom part, wherein the membrane filter has a reinforcing edge, and wherein the membrane filter can be clamped to a clamping part of a cover of a culture medium unit for removal purposes and introduced into the culture media unit. The invention further relates to a method for the microbiological analysis of liquid samples following a filtration process, wherein after removing an attachment a membrane filter is lifted off a filter support for pouring in the liquid sample and set down on a surface of a culture medium disposed in the bottom part of a culture medium unit, and the bottom part is covered by a cover. The cover is placed on the membrane resting on the filter support such that a clamping part present on the filter support clamps to a reinforcing edge of the membrane. The cover is lifted off the filter support together with the membrane and placed on the bowl-shaped bottom part such that the bottom of the membrane facing away from the cover rests on the top of the nutrient medium facing the cover.

The invention discloses a large-volume enrichment liquid aerosol liquid sampler suitable for air microbe analysis. The large-volume enrichment liquid aerosol liquid sampler is mainly used for correlation analysis of air microbes, and can be in single-stage or multi-stage series with enrichment bottles; large-volume enrichment liquid can be filled in each enrichment bottle, the large-volume enrichment liquid aerosol liquid sampler is characterized in that the enrichment of more microbes is conducted, a gas jet column is arranged at the bottom of the sampler, an air inlet is connected with a flowmeter, aerosol firstly enters the first stage of single-stage or multi-stage enrichment bottles; if the multi-stage series enrichment bottles are arranged, the air microbes not eluting in the first stage enrichment bottle can enter the second stage enrichment bottle again or more-stage enrichment liquid after the second stage enrichment bottle is further fully eluted; and the last stage enrichment bottle of single-stage or multi-stage enrichment bottles is connected with a diaphragm vacuum pump, and the enrichment liquid of the enrichment microbes is collected from the outlets under the enrichment bottles. According to the aerosol liquid sampler, the long-term sampling without obvious microbe secondary proliferation can be realized; samples can guarantee the microbe activity and culturability, and can guarantee the high-quality DNA extraction; and the microbial concentration at sampling sites is not limited, adaptable sampling types are wide, simple and efficient, and the production cost is low.

The invention discloses a method for automatically surveying and mapping the growth curve of a microorganism in food. The method has the main contributions that a novel method that the microbiological analysis is carried out by surveying and mapping the growth curve of the microorganism is proposed starting with the growth rule of the microorganism in the food and is a technological innovation on the traditional analytical method; the condition that the growth information of the microorganism can be converted into measurable frequency information by a piezoelectric acoustic wave sensor is discovered through experimental study, and thus, a design principle for designing a fast and efficient novel surveying and mapping device is provided; and a device for automatically surveying and mapping the growth curve of the microorganism in the food is designed and constructed under the guidance of the novel method and the design principle. The device constructed according to the method has the advantages that the real-time and automatic surveying and mapping of the growth curve of the microorganism in the food can be realized, and the in-depth study on the microorganism in the food, such as the study of food processing methods, food shelf lives and microbial antimicrobial activity, can be carried out according to the obtained growth curve; and as the device has the characteristics of simplicity and convenience in operation, accuracy in result, and the like, the device is hopeful to be popularized and applied in microorganism-related research of food processing, storage and selling.

Methods are presented for binning metagenomic sequences that leverage long reads from a single-molecule long-read sequencing technology and utilize DNA methvlation signatures inferred from these readsto resolve individual reads and assembled contigs into species- and strain-level clusters. Methods for deconvolving prokaryotic organisms in a microbiome sample are presented. Methods for mapping mobile genetic elements to their host organisms in a microbiome sample are also presented.

The invention discloses a microorganism analytical sampling device. The microorganism analytical sampling device comprises a cup-shaped body with the open upper end; an opening of the cup-shaped body is provided with a cover body; the cover body is matched with the opening of the cup-shaped body in size and shape; the bottom of the cup-shaped body is arranged into a funnel-shaped cup bottom; the middle of the funnel-shaped cup bottom is provided with a bottom end through hole; a bottom filter film is arranged below the bottom end through hole. According to the microorganism analytical sampling device, the structure is reasonably improved, the funnel-shaped cup bottom is matched with the bottom filter film particularly, and accordingly sputum can be conveniently sampled through the microorganism analytical sampling device without special air extraction device and accordingly the operation is convenient, the medical staff is well protected, and the significance on the sampling of dangerous bacteria such as tubercle bacilli is important.

The present invention provides an apparatus comprised of devices for simple, fast and cost-effective storage and plating of solid nutrient medium (SNM) for various uses in the field of microbiological analysis. The devices consist of a container which can be aseptically filled with SNM and equipped with a plunger to accurately press out needed amounts of the SNM; a cutting means to cut, hold and transfer SNM; a measuring means to measure the amount of SNM exiting the container; and a growth plate for storage of SNM, growth of microorganisms on SNM, and introduction of indicator substances to SNM for analysis of microorganisms.

The invention provides an erythrocyte aggregation detection method and a system thereof, which relate to the fields of biological and microbiological analysis detection. The method comprises the following steps: connecting an analysis module with an image information acquisition module and a control module for analyzing an obtained microscopic image; performing edge detection on erythrocyte in themicroscopic image, filling up an internal gas of the edge to form a complete erythrocyte shape image; performing a shrinkage analysis on an outer contour of the erythrocyte, and when the result is higher than a set value, determining the result as erythrocyte aggregation. calculating a ratio of an erythrocyte area with aggregation and an average area of the normal erythrocyte, and determining theerythrocyte aggregation degree according to the ratio. Compared with the prior art, the method employs a direct observation method and an image analysis method, the erythrocyte can be automatically identified, the area of an erythrocyte image and the area of the normal erythrocyte are compared, the erythrocyte aggregation degree is determined, the operation is convenient, the measurement efficiency is high, the identification precision is high, and the method can be used for quantitative analysis.

An apparatus for enhancing the contact frequency between two reactants capable of binding to one another, preferably between an analyzer molecule and an analyte molecule, especially for enhancing the binding effectiveness in bioanalysis arrays with the aid of micro- or nanomagnetic particles (75) set in motion in a controlled manner in the fluid reaction medium by means of magnetic fields generated by variably feedable electromagnets (3, 2, 20) arranged on both sides of the reaction fluid film. On one side of a reaction fluid film, especially of a microscope slide (4) comprising the reactants (63), the reaction liquid (70) comprising the reactants (73) and preferably a glass plate (5) covering the microbioanalysis array (6), is arranged close to a two-dimensional matrix (20) having a multitude of miniature or millimagnetic coils (2) feedable individually with magnetization current of variable strength and / or voltage as a function of time—corresponding to a time-dependent variable magnetization pattern desired in each case—and, on the other side of the reaction vessel, especially the microscope slide (4) with the (micro)bioanalysis array (6), in whose vicinity is positioned only one magnetic coil (3) likewise feedable with variable magnetization current and whose magnetic field permeates the entire reaction vessel, especially the microbioanalysis array (6).

The method provides a simple, accurate and inexpensive way to prepare culture media for conducting microbiological analysis with an absolute minimum of facilities, extraneous equipment and time consuming procedures. The invention introduces the use of a carrier piece that is suitable for carrying a reactant material for combining with growth media. The carrier piece may be formed using (non-)porous, (non-)absorbent material, including paper, plastic, gum, fabric, or acetate. The carrier piece carries a single one or a combination of various reactant materials that are released into the medium or medium / test sample mixture upon contact therewith. The reactant materials may include, among other things, nutrients, inhibitors, including antibiotics and bile salts, enzyme substrates, and / or a catalyst for a gelling agent contained in the growth medium. The growth medium, the carrier piece with the appropriate reactant material(s), and the test sample can be easily packaged and sold separately or as a kit and used anywhere.

The present invention hereby describes an apparatus and / or apparatuses to collect aqueous solutions, gas compounds, solid compounds and / or hydrocarbon liquid samples from a pipe, pipeline, tank or vessel for microbiological analysis; to determine if that environment contains microbiologically influenced corrosion (MIC) bacterium, which may contribute to the internal wall corrosion of the pipe's, pipeline's, tank's or vessel's metallic composition. The present invention also includes sterile absorbent materials within the apparatus(es); which have different absorbency rates and coatings, which are hydrocarbon and / or aqueous soluble at differing time periods, to represent adequate sampling of the fluids; as it travels through and within a pipe, pipeline, tank or vessel system. Solids are trapped within, and included within, a coated or non-coated sterile matrix which may contain sessilic MIC bacterium. Gas permeates the sterile matrix withholding contained planktonic bacterium thus providing a heterogeneous sampling of the contained contents.