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Large-volume enrichment liquid aerosol liquid sampler suitable for air microbe analysis

A technology of air microorganisms and enrichment liquid, which is applied in the direction of biological material sampling methods, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of aerosols without forming standards, reduce sampling efficiency and increase contact time Effect

Active Publication Date: 2019-10-29
NANKAI UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no established standards for aerosol sampling methods and sampling efficiency

Method used

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  • Large-volume enrichment liquid aerosol liquid sampler suitable for air microbe analysis
  • Large-volume enrichment liquid aerosol liquid sampler suitable for air microbe analysis
  • Large-volume enrichment liquid aerosol liquid sampler suitable for air microbe analysis

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Embodiment 1

[0027] Such as figure 1 As shown, the present invention (taking the setting of two-stage enrichment bottles as an example) includes two-stage enrichment bottles composed of first-stage enrichment bottles 3 and second-stage enrichment bottles 11, and its special feature is that each stage of enrichment Both bottles can contain a super-large volume of enrichment solution to enrich more microorganisms. The top of the two-stage enrichment bottles is equipped with an enrichment solution inlet 2. The enrichment solution in the enrichment bottle can be sterilized buffer solution or water. Including an air inlet, the air inlet is connected to the flowmeter 8 and the first-stage enrichment bottle 3 is connected, and the first-stage enrichment bottle 3 is connected to the second-stage enrichment bottle 11 through the air pipe 1; the inner bottom of each stage of enrichment bottle A gas injection column 6 is provided, and a enrichment liquid collection port 7 is provided at the outer bo...

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Abstract

The invention discloses a large-volume enrichment liquid aerosol liquid sampler suitable for air microbe analysis. The large-volume enrichment liquid aerosol liquid sampler is mainly used for correlation analysis of air microbes, and can be in single-stage or multi-stage series with enrichment bottles; large-volume enrichment liquid can be filled in each enrichment bottle, the large-volume enrichment liquid aerosol liquid sampler is characterized in that the enrichment of more microbes is conducted, a gas jet column is arranged at the bottom of the sampler, an air inlet is connected with a flowmeter, aerosol firstly enters the first stage of single-stage or multi-stage enrichment bottles; if the multi-stage series enrichment bottles are arranged, the air microbes not eluting in the first stage enrichment bottle can enter the second stage enrichment bottle again or more-stage enrichment liquid after the second stage enrichment bottle is further fully eluted; and the last stage enrichment bottle of single-stage or multi-stage enrichment bottles is connected with a diaphragm vacuum pump, and the enrichment liquid of the enrichment microbes is collected from the outlets under the enrichment bottles. According to the aerosol liquid sampler, the long-term sampling without obvious microbe secondary proliferation can be realized; samples can guarantee the microbe activity and culturability, and can guarantee the high-quality DNA extraction; and the microbial concentration at sampling sites is not limited, adaptable sampling types are wide, simple and efficient, and the production cost is low.

Description

technical field [0001] The invention belongs to the field of microbial aerosol sampling, in particular to an aerosol liquid sampler suitable for air microbial analysis of large-volume enriched liquid aerosols. Background technique [0002] At present, for the analysis of air microorganisms, large-flow membrane samplers and commercially available liquid samplers have been widely used to collect aerosol samples; although large-flow membrane samplers can increase the amount of DNA extraction to a certain extent, they cannot Guarantee microbial activity and cultivability; commercially available liquid samplers have a large flow rate and small volume of enrichment solution, and there is cross-contamination when updating the enrichment solution. Liquid elution efficiency is low, causing microbial loss and other problems. [0003] The aerosol sampler with high capture efficiency can meet the requirements of 16S rRNA high-throughput sequencing, qPCR (quantitative polymerase chain r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C12M1/32
CPCC12M33/04C12M33/06
Inventor 毛大庆赵艳辉罗义
Owner NANKAI UNIV
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