A bioreactor equipped with a microfluidic structure

Abstract

The invention discloses a bioreaction device equipped with a microfluidic structure, including an extraction chamber, a reaction chamber, and a microfluidic structure for transferring macromolecular extracts in the extraction chamber to the reaction chamber; above the microfluidic structure, a An air pressure pump, the microfluidic structure is provided with an air guide channel connecting the extraction chamber and the air pressure pump, and an extraction liquid channel connecting the extraction chamber and the reaction chamber; one end of the extraction liquid channel is arranged in the extraction chamber The liquid conduction tip is connected with each other, and the other end of the extraction liquid channel is connected with the liquid conduit arranged in the reaction chamber. The biological reaction device provided with a microfluidic structure of the present invention is provided with a microfluidic structure connected to the extraction chamber and the reaction chamber, and the extracted materials (protein, nucleic acid) are fully and efficiently transferred from the extraction chamber to the reaction chamber through the microfluidic structure. In the warehouse, the experimental process is simplified to improve the experimental efficiency and reduce the possibility of contamination during sample transfer. Not only is its structure simple, but it is also easy to operate, which can effectively reduce the complexity of the equipment used in conjunction with it.

Description

technical field [0001] The invention relates to the technical fields of biochemistry and molecular biology, in particular to a biological reaction device provided with a microfluidic structure. Background technique [0002] With the transformation of the medical model and the continuous development of individualized medicine, the medical testing community urgently needs fast and accurate detection methods, and molecular detection plays a unique role. The basis of molecular detection is to analyze the genes and their expression products in the tissue cells, hair, anticoagulant blood or dried blood stains of the subject, as well as formaldehyde-fixed and paraffin-embedded tissues. ) detection can be accurately detected once the disease occurs or even before the symptoms, signs and biochemical changes appear. [0003] At present, molecular detection technologies mainly include nucleic acid molecular hybridization, polymerase chain reaction (PCR) and biochip technology. Molecu...

AI Technical Summary

Problems solved by technology

At present, blood routine, cytology, pathology and immunology and other testing methods are all developing in the direction of automation, integration and standardization, but due to the technical complexity of molecular testing, the fully automated instrument platform "from sample to result" Few or many technical problems that are difficult to solve

For example, the spin column method or magnetic bead method used in the field of biotechnology for nucleic acid extraction generally requires four steps of lysis, binding, rinsing, and elution, plus subsequent nucleic acid molecule hybridization, polymerase chain reaction (PCR ) and biochips and other detection steps, it is very difficult to realize the fully automated instrument "from sample to result". It is obvious that the manual material transfer method used in the prior art is not only cumbersome to operate, but also time-consuming and labor-intensive.

What's more, the whole operation process is easy to cause pollution, which affects the purity of the extract, and it is difficult to transfer the material fully and efficiently, which affects the experimental results.

Therefore, in view of the technical complexity of molecular detection itself, there are few fully automated instrument platforms for "from sample to result" or there are many difficult technical problems. In order to avoid problems in the prior art such as high cost, poor sensitivity, complex structure, overly complicated operation and related detection equipment, etc.

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