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Microbial index evaluation method for health condition of mangrove wetland sediment

A technology for health status and mangroves, applied in biochemical equipment and methods, microbial determination/inspection, resources, etc., can solve problems such as strong subjectivity, inaccurate results, and complicated calculations, and achieve more accurate evaluation results. , to avoid the effect of limitations

Pending Publication Date: 2019-10-01
PEKING UNIV SHENZHEN GRADUATE SCHOOL
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Problems solved by technology

[0005] In order to overcome the deficiencies in the prior art, the object of the present invention is to provide a method for evaluating the microbial index of the health status of mangrove wetland sediments, which can overcome the inaccurate results, complex calculations and subjectivity in traditional monitoring methods. To accurately assess the health status of mangrove sediments through the abundance, diversity and community structure of microbial communities, provide basic data support and point out the direction of protection for mangrove protection and management

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  • Microbial index evaluation method for health condition of mangrove wetland sediment
  • Microbial index evaluation method for health condition of mangrove wetland sediment
  • Microbial index evaluation method for health condition of mangrove wetland sediment

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specific Embodiment 1

[0068] In this example, Xixiang mangrove and Shajing mangrove are selected as areas to be evaluated to evaluate the health status of their sediments.

[0069] Survey on the status quo of mangrove wetlands. Xixiang Mangrove (E113°50′36″, N22°35′33″) and Shajing Mangrove (E113°45′56″, N22°43′37″) are both located in Shenzhen City, Guangdong Province, with an average temperature of 22°C. The average rainfall is 1927mm, the average humidity is 79%, the tide is irregular semi-diurnal tide, and the average tidal range is 1.9m. The mangrove plant planted in the Xixiang mangrove forest is candela, the oxygen reduction potential of the surface sediment (0-10cm) of the sediment is -91.0±20.0mV, and the organic matter content is 43.00gkg -1, the total nitrogen content is 2.27g / kg, the total phosphorus content is 0.86g / kg, and the sulfate content is 1.80g / kg. The mangrove plant planted in Shajing mangrove forest is candela, the oxygen reduction potential of the surface sediment (0-10cm)...

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Abstract

The invention provides a microbial index evaluation method for the health condition of mangrove wetland sediment and belongs to the field of environmental monitoring. The method comprises the steps ofinvestigating the current situation of a mangrove wetland; determining indicator microorganisms; selecting ideal reference points; establishing a mangrove sediment sample collection method and determining microbial analysis methods, wherein the microbial analysis methods comprise a sample DNA extraction method, a PCR amplification method and a clone library construction method; selecting an indicator microorganism index evaluation factor and adopting a gene copy number of the indicator microorganisms, a diversity index and the proportion of dominant flora as evaluation factors; determining the weight Wi of the microorganism index evaluation factors; calculating an evaluation ratio Ii of the microbial index evaluation factors, and calculating an evaluation index P; determining the evaluation grade, and evaluating the health condition of the mangrove wetland sediment according to the evaluation grade. The provided microbial index evaluation method for the health condition of the mangrove wetland sediment can be used for accurately and effectively evaluating the health condition of the mangrove wetland sediment under field conditions and is wide in application range.

Description

technical field [0001] The invention relates to the technical field of environmental monitoring, in particular to a microbial index evaluation method for the health status of mangrove wetland sediments polluted by heavy metals. Background technique [0002] Mangroves are woody plant communities commonly found in the intertidal zone of tropical-subtropical low-tidal energy coasts. Mangrove sediments are environments rich in organic matter and nutrients, making mangrove sediments rich in microbial communities. In recent years, with the rapid development of coastal urbanization, a large amount of industrial wastewater has been discharged into the mangrove ecosystem, causing serious harm to the health of mangrove sediments. Industrial wastewater contains a large amount of heavy metal pollutants, which make the heavy metal pollutants enriched in mangrove sediments have a direct toxic effect on benthic organisms or organisms that depend on sediments for survival. Therefore, the h...

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Application Information

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IPC IPC(8): C12Q1/6851C12Q1/6869G06Q10/06G06Q50/26
CPCC12Q1/6851C12Q1/6869G06Q10/06393G06Q50/26C12Q2531/113C12Q2563/107C12Q2545/114
Inventor 李瑞利吴思颉沈小雪
Owner PEKING UNIV SHENZHEN GRADUATE SCHOOL
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