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Method for fixing acidophilic bacteria

A fixation method and acidophilic technology, which is applied in the field of preparation of eosinophilic bacterial spherical carriers, can solve the problems of maintaining the acidic growth environment of eosinophilic bacteria, irregular spherical particles, and cumbersome operation process, so as to achieve non-sticking of particles and save dosage , the effect of rapid reaction into balls

Inactive Publication Date: 2006-12-27
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the operation process of these methods is relatively cumbersome, which is not conducive to maintaining the acidic growth environment of acidophilic bacteria, and the spherical particles formed are not regular enough.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Weigh three sets of mixed gels in the following proportions:

[0014] PVA (degree of polymerization, 1750±50) 6g, sodium alginate (chemically pure) 1.5g;

[0015] PVA (degree of polymerization, 1750±50) 8g, sodium alginate (chemically pure) 1g;

[0016] PVA (degree of polymerization, 1750±50) 12g, sodium alginate (chemically pure) 0.8g;

[0017] The three groups of gels were put into 90ml of deionized water and heated to dissolve in a boiling water bath. After cooling, 10ml of Thiobacillus ferrooxidans cell suspension was added respectively, and the final cell concentration was 1.5×10 9 individual / ml, the concentrations of PVA in groups 1-3 were 6%, 8%, and 12%, respectively, and the concentrations of sodium alginate in groups 1-3 were 1.5%, 1.0%, and 0.8%, respectively. The above mixed sol was added dropwise to (NH 4 ) 2 SO 4 -H 2 SO 4 buffer solution, in which (NH 4 ) 2 SO 4 The concentration (w / v) is 25%, a certain amount of concentrated sulfuric acid is ad...

Embodiment 2

[0020] Weigh 7.5g of PVA (degree of polymerization, 1750±50) and 1g of sodium alginate (chemically pure), put them in 90ml of deionized water, and heat them in a boiling water bath to dissolve them. After cooling, add 10ml of Thiobacillus ferrooxidans cell suspension respectively. Cell concentration 1.5×10 9 pcs / ml, the concentration of PVA is 7.5%, and the concentration of sodium alginate is 1.0%, respectively. The above mixed sols were added dropwise to the two (NH 4 ) 2 SO 4 -H 2 SO 4 In buffer solution, two buffers (NH 4 ) 2 SO 4 The concentration (w / v) of the solution is 10% and 70%, respectively, add a certain amount of concentrated sulfuric acid to make the final pH of the buffer solution 1.5, gently stir the buffer solution with a magnetic stirrer while adding dropwise, and soak in the particle solution for 40min Shaped (average particle diameter 3.5mm), rinsed with deionized water for several times, and used for later use.

[0021] After the above two kinds o...

Embodiment 3

[0023] Weigh 9g of PVA (degree of polymerization, 1750±50) and 0.9g of sodium alginate (chemically pure), put them in 90ml of deionized water, and heat them in a boiling water bath. After cooling, add 10ml of Thiobacillus ferrooxidans cell suspension respectively. Cell concentration 1.5×10 9 pcs / ml, the concentration of PVA was 9%, and the concentration of sodium alginate was 0.9%, respectively. The above mixed sol was added dropwise to the two kinds of K 2 SO 4 -H 2 SO 4 In buffer solution, K 2 SO 4 The concentration (w / v) is 40%, and a certain amount of concentrated sulfuric acid is added to each, so that the final pH of the two buffer solutions is 1.0 and 3.5, respectively, and the buffer solution is gently stirred with a magnetic stirrer while adding dropwise. Soak for 1.5h to form (average particle diameter of 3.5mm), rinse with deionized water for several times, and set aside.

[0024] After the above two kinds of particles were activated in a series of shake flas...

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Abstract

The invention relates to an acidophile fixation method. The method includes heating the polyvinyl alcohol (PVA) and sodium alginate with finite concentration ratio and consolving in the water, after cooling, mixing with acidophile, directly dripping the said miscible liquids into sulfate -dipping acid buffer solution, reacting them to form particles, and obtaining granulation carrier after desalting with deionized water. The new method for preparation of spherical carrier can be widely used in acidophilic microorganism immobilization.

Description

Technical field [0001] The invention relates to a preparation technology of biologically immobilized particles, in particular to a preparation method of a spherical carrier of acidophilic bacteria. Background technique [0002] Acidophilic bacteria, such as Thiobacillus ferrooxidans, Thiobacillus denitrificans, Thiobacillus thiobacillus, Thiobacillus acidophilus, etc. need to grow in an environment with strong acidity, and usually have strong acid resistance. Tests have shown that using some conventional The biological activity of bacteria is not very high, such as agar embedding method, calcium alginate embedding method, polyacrylamide embedding method, carrageenan embedding method, gelatin-glutaraldehyde embedding method, etc. , limiting the application of such bacteria in immobilization. [0003] In contrast, PVA has a series of advantages such as high strength, good chemical stability, strong anti-microbial decomposition performance, non-toxicity to microorganisms, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/04C12N1/20
Inventor 李红玉王玉建支德娟
Owner LANZHOU UNIVERSITY
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