Extraction method of acidophilic bacteria genome DNA in wet method metallurgy

Abstract

The invention provides a novel, fast and stable method for extracting genome DNA from pulp acidophilic bacteria. It comprises following steps: centrifuging cultured pulp, washing with 0.2 M sulfate and SET buffer solution respectively; adding lysozyme and treating for 30 minutes at 37 Deg. C, adding protein enzyme K and SDS and treating for 45 minutes at 50 Deg. C, adding glass balls and vortexing for 1 minute to crack cell completely, then extrcting protein with chloroform, washing with waterless alcohol precipitation and alcohol of 75% and getting pure genome DNA, which can be used for PCR gene operation.

Description

technical field [0001] The invention relates to a method for extracting acidophilic bacteria genome DNA. technical background [0002] The method of using acidophilus to dissolve and recover metals from lean ore and tailings is called biohydrometallurgy. Copper recovery from mine leach water has been known since the 18th century at the Rio Tinto mines in Spain. Biohydrometallurgy was officially recognized as a technology in the 1950s after the isolation and identification of Thiobacillus ferrooxidans. In 1958, the United States began to use biohydrometallurgy to extract copper. In 1966, Canada began to extract uranium, and then wet extraction of zinc, cobalt, nickel, gold and other metals was also successful. Biohydrometallurgy is widely used all over the world. In 1997 and 2001, my country also built two biological copper heap leaching plants with a capacity of more than 1,000 tons in the Dexing Copper Mine in Jiangxi and the Zijinshan Copper Mine in Fujian. In the past ...

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Problems solved by technology

However, the acidophilic bacteria in hydrometallurgy exist in the slurry whose pH is lower than 2.0 in the reactor, and the existence state is very special, so the methods used to extract the genomic

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