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Preparation method of antarctic krill phospholipid

An Antarctic krill phospholipid, Antarctic krill technology, applied in the direction of producing fat, fat oil/fat production, etc., can solve the problems of Antarctic krill transportation, storage difficulties, lack of Antarctic krill phospholipid processing and preparation methods, etc., to achieve suitable promotion, Excellent product purity and quality, the effect of reducing production costs

Inactive Publication Date: 2012-07-11
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To solve the problem of Antarctic krill transportation and storage difficulties, the best way is to quickly process Antarctic krill to prepare phospholipids after harvesting, but there is currently no on-board processing and preparation method for Antarctic krill phospholipids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of preparation method of Antarctic krill phospholipid comprises the steps:

[0025] 1 Raw materials and processing: Drain the fresh and live Antarctic krill and homogenize directly;

[0026] 2. Low-temperature autolysis: The Antarctic krill homogenate was irradiated with 200-nanometer ultraviolet rays with a power of 40 watts and a distance of 0.5 meters for 10 minutes, and the treated homogenate was autolyzed for 2 hours at the fishing environment temperature;

[0027] 3. Ultrasound-assisted exogenous enzymatic hydrolysis: add 1 times the volume of water to the homogenate, treat with 300 watts of ultrasonic waves (200 nm) for 5 minutes, adjust the pH to 7.5 with 1M HCl or NaOH, and add alkaline to the homogenate Protease (9×10 5 U / kg), mixed evenly, and enzymatically hydrolyzed at 60°C for 1 hour; the sample hydrolyzed by exogenous enzyme was spray-dried to obtain a dry powder.

[0028] 4. Extraction of Antarctic krill phospholipids: Add 5 times ...

Embodiment 2

[0029] Embodiment 2: a kind of preparation method of Antarctic krill phospholipid comprises the steps:

[0030] 1 Raw materials and processing: Drain the fresh and live Antarctic krill and homogenize directly;

[0031] 2. Low-temperature autolysis: The Antarctic krill homogenate was irradiated with 200-nanometer ultraviolet rays with a power of 20 watts and a distance of 1 meter for 20 minutes, and the treated homogenate was autolyzed for 10 hours at the fishing environment temperature;

[0032] 3. Ultrasound-assisted exogenous enzymatic hydrolysis: add 3 times the volume of water to the homogenate, treat with 800 watts of ultrasonic waves (300 nm) for 10 minutes, adjust the pH to 8.0 with 2M HCl or NaOH, and add alkaline to the homogenate Protease (10×10 5 U / kg), mixed evenly, and hydrolyzed at 55°C for 4 hours; the samples hydrolyzed by exogenous enzymes were spray-dried to obtain dry powder.

[0033] 4. Extraction of Antarctic krill phospholipids: add 15 times the volume ...

Embodiment 3

[0034] Embodiment 3: a kind of preparation method of Antarctic krill phospholipid comprises the steps:

[0035] 1 Raw materials and processing: Drain the fresh and live Antarctic krill and homogenize directly;

[0036] 2. Low-temperature autolysis: The Antarctic krill homogenate was irradiated with ultraviolet rays with a power of 30 watts and a wavelength of 200 nanometers at a distance of 0.8 meters for 30 minutes, and the processed homogenate was autolyzed for 7 hours at the fishing environment temperature;

[0037] 3. Ultrasound-assisted exogenous enzymatic hydrolysis: add 2 times the volume of water to the homogenate, treat with 550 watts of ultrasonic waves (wavelength 400 nm) for 15 minutes, adjust the pH to 7.75 with 3M HCl or NaOH, and add alkali to the homogenate Protease (9.5×10 5 U / kg), mixed evenly, and enzymatically hydrolyzed at 65°C for 1 hour; the sample hydrolyzed by exogenous enzyme was spray-dried to obtain a dry powder.

[0038] 4. Extraction of Antarcti...

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Abstract

The invention discloses a preparation method of antarctic krill phospholipid. The preparation method comprises the steps: 1, draining and drying the antarctic krill and homogenizing; 2, irradiating homogenate liquid by using ultraviolet rays for 10-30 mins and autolyzing for 2-10 hours; 3, adding water into the homogenate liquid, ultrasonically processing, adjusting the pH value to be 7.5-8.0, adding alkali protease, and enzymatically hydrolyzing to obtain enzymolysis liquid; and drying the enzymolysis liquid to obtain dry powder; and 4, extracting the antarctic krill phospholipid by utilizing an ethanol extraction method. The preparation method is particularly suitable for use after fresh and alive antarctic krill are fished by fishing ships, and is simple in operation and high in efficiency. Particularly, the usage amount of exogenous enzymes is reduced by utilizing the strong autolysis capability of the fresh and alive antarctic krill, so that the production cost is lowered. Moderate conditions are provided for the extraction method, so that original physiological activators in the antarctic krill phospholipid can be stored to the maximum extent, and products are endowed with both nutritional and functional properties.

Description

technical field [0001] The invention relates to a method for extracting Antarctic krill phospholipids. Background technique [0002] Antarctic krill (Euphausia superba) is one of the largest single species of biological resources on the earth. Its existing stock is estimated to be about 400-1.5 billion tons, and the annual output of mature shrimp is 300-500 million tons. About 100 million tons, forming a huge potential fishery resource. In recent years, with the gradual depletion of world-wide traditional fishery resources and the proposal of a 200 nautical mile exclusive economic zone, the huge Antarctic krill resources in Antarctic waters have attracted the attention of some pelagic fishery developed countries. my country has also included Antarctic krill resources as one of the main development species for the future development of pelagic fisheries. [0003] At present, the development of Antarctic krill resources is mainly based on the utilization of its protein, but ...

Claims

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Application Information

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IPC IPC(8): C11B1/02C11B1/10
Inventor 朱蓓薇周大勇吴海涛米云龙孙黎明
Owner DALIAN POLYTECHNIC UNIVERSITY
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