A synergistic composition for controlling mango anthracnose

Abstract

The present application belongs to the technical field of pesticides, and particularly relates to a synergistic composition for preventing and treating mango anthracnose. The synergistic fungicidal composition is prepared by compounding compound I and compound II in a mass ratio of 1-200:200-1, wherein the compound I is limettin enol, and the compound II is prochloraz, silthiofam or mancozeb. The limettin enol, the prochloraz, the silthiofam or the mancozeb are compounded in a specific mass ratio range, have a synergistic effect on inhibiting the mango anthracnose pathogen, and can improve the prevention and treatment effect on the mango anthracnose. On this basis, the application amount of the pesticide can be reduced, and the environmental pollution and pesticide residues can be reduced. In addition, when the limettin enol, the prochloraz, the silthiofam or the mancozeb are compounded, the occurrence and development of the pathogen resistance can be delayed, and the risk of resistance can be reduced.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pesticides, and particularly relates to a synergistic composition for preventing and treating mango anthracnose. BACKGROUND

[0002] Mango is a tropical fruit with high economic value. The fruit is rich in nutrients and has a unique flavor, containing rich carbohydrates, sugars, proteins, cellulose and trace elements. In the process of mango production, many diseases can affect its healthy growth, and mango anthracnose caused by Colletotrichum gloeosporioides is one of the main diseases. Mango anthracnose is the first fungal disease of mango, and the incidence rate can reach 100% under high temperature and high humidity environment, which seriously affects the development of mango industry.

[0003] At present, the main prevention and control method for mango anthracnose in production is chemical control, such as spraying carbendazim, thiophanate-methyl and prochloraz. With the widespread, excessive and unscientific use of these chemical pesticides, the pathogenic bacteria resistance has been developed, which in turn leads to environmental pollution and food safety problems. Therefore, it is particularly important to find a safe and efficient agent for preventing and treating mango anthracnose.

[0004] Auraptene, also known as 8-(2-hydroxy-3-methyl-3-butenyl)-7-methoxycoumarin, CAS: 1221-43-8, molecular formula: C 15 H 16 O4, and the molecular structure formula is as follows

[0005]

[0006] According to relevant reports, Auraptene has bactericidal activity. Chinese patent CN201410009288.9 discloses the use of a natural compound as a crop agricultural fungicide. Auraptene is disclosed in the patent, and it is also disclosed that Auraptene can effectively prevent and control Sclerotinia sclerotiorum, cucumber fusarium wilt, rice sheath blight, pepper anthracnose, rice sheath blight, wheat sheath blight, tomato late blight and gray mold, and the inhibition concentration EC 50 is 2.84-18.46 ppm. Auraptene has a wide inhibition spectrum and a long effective period, and is an ideal agricultural fungicide. Compared with general chemical pesticides, Auraptene is extracted from natural plants, has the characteristics of low toxicity, no residue, no pollution and no public hazard, and is more beneficial to environmental protection.

[0007] The different pesticide ingredients are compounded to be a convenient way for developing new pesticides, and there is no related report of the compound of limettin enol, prochloraz, fluopicolide or mancozeb. SUMMARY

[0008] The present application aims to provide a synergistic composition for preventing and treating mango anthracnose.

[0009] To achieve the above object, the present application provides the following technical scheme.

[0010] A fungicidal synergistic composition, wherein the effective component of the fungicidal synergistic composition is compounded by compound I and compound II with a mass ratio of 1-200:200-1, wherein the compound I is limettin enol, and the compound II is prochloraz, fluopicolide or mancozeb.

[0011] As a preferred, when the compound II is prochloraz, the mass ratio of the compound I and the compound II is 1-8:16-1.

[0012] As a preferred, when the compound II is fluopicolide, the mass ratio of the compound I and the compound II is 1-5:9-1.

[0013] As a preferred, when the compound II is mancozeb, the mass ratio of the compound I and the compound II is 1-12:7-1.

[0014] As a preferred, the mass ratio of the compound I and the compound II is 3:1.

[0015] The present application also provides the application of the fungicidal synergistic composition in preventing and treating mango anthracnose.

[0016] Compared with the prior art, the present application has the following beneficial effects:

[0017] (1) The limettin enol, prochloraz, fluopicolide or mancozeb in the present application is compounded in a specific mass ratio range, has a synergistic effect on inhibiting the mango anthracnose pathogenic bacteria, and can improve the prevention and treatment effect on mango anthracnose. On this basis, the application amount of pesticides can be reduced, and the environmental pollution and pesticide residues can be reduced.

[0018] (2) When the limettin enol, prochloraz, fluopicolide or mancozeb is compounded, the occurrence and development of the drug resistance of the pathogenic bacteria can be delayed, and the risk of drug resistance can be reduced. DETAILED DESCRIPTION

[0019] The following clearly and completely describes the technical solutions of the patent application. Obviously, the described embodiments are part of the embodiments of the present application, rather than all embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of the present application.

[0020] Example : Indoor bioactivity test

[0021] 1. Test strain: Colletotrichum gloeosporioides isolated from diseased mango fruit and preserved on PDA medium.

[0022] 2. Test agents

[0023] 98% limonene original drug (Shanghai Yuan Ye Biotechnology Co., Ltd.), 95% prochloraz original drug (Inner Mongolia Laike Crop Protection Co., Ltd.), 92% pyraclostrobin original drug (Syngenta Nantong Crop Protection Co., Ltd.), 85% mancozeb original drug (Zhengzhou Xianlida Chemical Co., Ltd.)

[0024] The test agent is first dissolved in dimethyl sulfoxide, then diluted with 0.1% Tween-80 aqueous solution to prepare a single dose stock solution, and multiple groups of ratios are set. Each single dose stock solution and the mixed agent of the ratio are set to have five mass concentration gradients by equal ratio method.

[0025] 3. Test method

[0026] The mycelial growth rate method is used. 9 mL of pre-melted PDA medium is added to a sterile conical flask, 1 mL of drug solution is quantitatively taken from low concentration to high concentration, and is added to the above conical flask, respectively, and is shaken well before being poured into a culture dish with a diameter of 9 cm to prepare a drug-containing plate with corresponding concentration, and a treatment without drug is set as a blank control. Each treatment is set with 5 replicates. A puncher is used to cut a 5 mm diameter fungus cake at the edge of the test strain colony, which is inoculated in the center of the drug-containing plate and the blank control plate, respectively, and is placed in a 25℃ constant temperature incubator after covering with a dish cover. When the colony diameter of the blank control reaches two-thirds of the diameter of the culture dish, the colony diameter is measured by cross method, and the mycelial growth inhibition rate of different treatments is calculated.

[0027] 4. Data analysis: The DPS software is used for data statistical analysis, the logarithmic value of fungicide concentration is taken as x, and the corresponding mycelial growth inhibition rate probability value is taken as y for linear regression to obtain the virulence regression equation and the virulence EC 50 value of the agent to the target pathogen, and the co-toxicity coefficient (CTC) is calculated according to Sun Yunpei method.

[0028] 5. Determination results

[0029] The synergistic effect of the agents was evaluated according to the calculated co-toxicity coefficient (CTC), and the results are shown in Tables 1-3.

[0030] Table 1 Indoor bioactivity determination of lime oil enol and prochloraz compound on Colletotrichum gloeosporioides

[0031] Fungicide name and ratio EC 50 (mg / L)]]> ATI TTI CTC Limonene 5.72 100.00 -- -- Prochloraz 8.63 66.28 -- -- Limonene 1 : Prochloraz 16 4.19 136.52 68.26 199.98 Limonene 1 : Prochloraz 12 5.31 107.72 68.87 156.40 Limonene 1 : Prochloraz 8 3.39 168.73 70.03 240.95 Limonene 1 : Prochloraz 4 4.42 129.41 73.02 177.22 Limonene 1 : Prochloraz 2 3.93 145.55 77.52 187.75 Limonene 1 : Prochloraz 1 2.97 192.59 83.14 231.65 Limonene 2 : Prochloraz 1 4.92 116.26 88.76 130.98 Limonene 4 : Prochloraz 1 3.81 150.13 93.26 160.99 Limonene 8 : Prochloraz 1 2.26 253.10 96.25 262.95

[0032] As shown in Table 1, in the mass range of 1-8:16-1, the co-toxicity coefficient of lime oil enol and prochloraz compound for inhibiting Colletotrichum gloeosporioides is greater than 120, showing a synergistic effect.

[0033] Table 2 Indoor bioactivity determination of lime oil enol and isopyrazam compound on Colletotrichum gloeosporioides

[0034] Fungicide name and ratio EC 50 (mg / L) ATI TTI CTC Limonene 5.72 100.00 -- -- Isopyrazam 2.20 260.00 -- -- Limonene 1 : Isopyrazam 9 1.64 348.78 244.00 142.94 Limonene 1 : Isopyrazam 7 1.31 436.64 240.00 181.93 Limonene 1 : Isopyrazam 5 1.82 314.29 233.33 134.69 Limonene 1 : Isopyrazam 3 0.97 589.69 220.00 268.04 Limonene 1 : Isopyrazam 1 2.49 229.72 180.00 127.62 Limonene 3 : Isopyrazam 1 3.18 179.87 140.00 128.48 Limonene 5 : Isopyrazam 1 2.72 210.29 126.67 166.02

[0035] As shown in Table 2, in the mass range of 1-5:9-1, the co-toxicity coefficient of lime oil enol and isopyrazam compound for inhibiting Colletotrichum gloeosporioides is greater than 120, showing a synergistic effect.

[0036] Table 3 Indoor bioactivity determination of lime oil enol and mancozeb compound on Colletotrichum gloeosporioides

[0037] Fungicide name and ratio EC 50 (mg / L) ATI TTI CTC Limonene 5.72 100.00 -- -- Mancozeb 3.06 186.93 -- -- Limonene 1 : Mancozeb 7 1.26 453.97 176.06 257.85 Limonene 1 : Mancozeb 5 2.71 211.07 172.44 122.40 Limonene 1 : Mancozeb 3 1.07 534.58 165.20 323.60 Limonene 1 : Mancozeb 1 1.50 381.33 143.46 265.80 Limonene 3 : Mancozeb 1 0.68 841.18 121.73 691.01 Limonene 5 : Mancozeb 1 2.37 241.35 114.49 210.81 Limonene 7 : Mancozeb 1 4.06 140.89 110.87 127.08 Limonene 9 : Mancozeb 1 4.27 133.96 108.69 123.24 Limonene 12 : Mancozeb 1 3.61 158.45 106.69 148.52

[0038] As shown in Table 3, in the mass range of 1-12:7-1, the co-toxicity coefficient of lime oil enol and mancozeb compound for inhibiting Colletotrichum gloeosporioides is greater than 120, showing a synergistic effect; when the mass ratio is 3:1, the co-toxicity coefficient reaches 691.01, and the synergistic effect is the most significant.

[0039] In summary, the lime oil enol, prochloraz, isopyrazam or mancozeb compound in a specific mass ratio range has a synergistic effect on inhibiting the mango anthracnose pathogen, and can improve the prevention and treatment effect on mango anthracnose. On this basis, the application amount of pesticides can be reduced, and environmental pollution and pesticide residues can be reduced.

Claims

1. A bactericidal and synergistic composition, characterized in that, The effective components of the bactericidal and synergistic composition are compounded from compound I and compound II, wherein compound I is hesperidinol, and compound II is imazalil, pyraclostrobin, or mancozeb; wherein... When compound II is imazalil, the mass ratio of compound I to compound II is 1-8:16-1; When compound II is pyraclostrobin, the mass ratio of compound I to compound II is 1-5:9-1; When compound II is mancozeb, the mass ratio of compound I to compound II is 1-12:7-1.

2. The application of the bactericidal and synergistic composition according to claim 1 in the prevention and control of mango anthracnose.

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