Eye spot brassica rapa adult feed and preparation method thereof

Abstract

The invention discloses a brassica rapa imago feed and a preparation method thereof, and belongs to the technical field of insect breeding. The Eye spot Brassica rapa adult feed comprises 11 to 15 parts of casein, 1 to 3 parts of Webster salt, 4 to 6 parts of cane sugar, 9 to 12 parts of cellulose powder, 0.2 to 0.4 part of L-cysteine, 0.2 to 0.3 part of choline chloride, 0.08 to 0.1 part of inositol, 0.325 to 0.4 part of composite vitamin, 2 to 3 parts of a nutritional ingredient ethanol solution, 12 to 14 parts of a nutritional ingredient aqueous solution, 1 to 2 parts of honey, 2 to 3 parts of agar powder, 0.03 to 0.04 part of cholesterol, 33 to 67 parts of apple jam, 0.3 to 4 parts of royal jelly and 80 to 120 parts of water. In addition, the invention also provides a preparation method of the feed for the Eye spot Brassica rapa imagoes. The feed for the Eye spot Brassica rapa adults can prolong the life of the adults and improve the egg laying amount at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of insect breeding, and particularly to an adult feed for Mylabris cichorii and a preparation method thereof. Background Art

[0002] Mylabris cichorii is an important medicinal insect, and cantharidin with anti-cancer efficacy is contained in its body, so it has extremely high application value in the medical field. However, the traditional breeding of Mylabris cichorii mainly relies on natural foods. For example, the larvae feed on locust eggs, and the adults rely on plants such as towel gourd flowers. This breeding method has many limitations, including high breeding costs, being restricted by seasons, and being difficult to produce on a large scale.

[0003] In recent years, in order to overcome these limitations, researchers have begun to explore artificial feed formulations for Mylabris cichorii. For example, a kind of artificial feed for adults has been developed, and its components include soybean powder, yeast powder, dried towel gourd flowers, multi-dimensional glucose, pollen, honey, etc. This feed can not only replace natural towel gourd flowers, but also meet the nutritional needs of adult Mylabris cichorii, and significantly reduce the breeding cost.

[0004] Although certain achievements have been made in existing research, there are still deficiencies in the existing technology. For example, a small amount of natural components (such as dried towel gourd flowers) still need to be added in some artificial feed formulations, and it is difficult to mass-produce natural components such as natural dried towel gourd flowers, which to a certain extent limits the large-scale breeding and cost control. In addition, how to further extend the adult lifespan and increase the egg-laying amount is still a key issue in current research. Summary of the Invention

[0005] The present invention aims to provide a brand-new adult feed for Mylabris cichorii and a preparation method thereof to solve the problems existing in the existing technology, and further promote the large-scale breeding of Mylabris cichorii and the stable production of cantharidin. By optimizing the feed formulation, the goal of the present invention is to significantly extend the adult lifespan and increase the egg-laying amount at the same time, providing technical support for the efficient breeding of adult Mylabris cichorii.

[0006] The purpose of the present invention is to overcome the above technical deficiencies, provide an adult feed for Mylabris cichorii and a preparation method thereof, and solve the technical problems of how to extend the adult lifespan and increase the egg-laying amount in the existing technology.

[0007] In order to achieve the above technical purpose, the technical solution of the present invention provides a feed for adults of eye-spotted meloides, which comprises, by weight, 11 to 15 parts of casein, 1 to 3 parts of Webster salt, 4 to 6 parts of sucrose, 9 to 12 parts of cellulose powder, 0.2 to 0.4 parts of L-cysteine, 0.2 to 0.3 parts of choline chloride, 0.08 to 0.1 parts of inositol, 0.325 to 0.4 parts of complex vitamins, 2 to 3 parts of ethanol solution of nutrient components, 0.10 to 0.13 parts of linoleic acid, 0.05 to 0.07 parts of linolenic acid, 12 to 14 parts of aqueous solution of nutrient components, 1 to 2 parts of honey, 2 to 3 parts of agar powder, 0.03 to 0.04 parts of cholesterol, 33 to 67 parts of applesauce, 0.3 to 4 parts of royal jelly and 80 to 120 parts of water.

[0008] In any embodiment, the composition further comprises 0.7 to 1 part of ascorbic acid and 0.2 to 0.4 part of sorbic acid.

[0009] In any embodiment, the nutrient component ethanol solution includes: 0.01-0.03 parts of dl-α-tocopherol acetate, 0.001-0.002 parts of vitamin D3, and 0.001-0.002 parts of menadione.

[0010] In any embodiment, the aqueous solution of nutrients includes: 0.002-0.003 parts of niacinamide, 0.002-0.003 parts of D-calcium pantothenate, 0.0005-0.0007 parts of thiamine, 0.001-0.002 parts of riboflavin, 0.0005-0.0007 parts of pyridoxine, 0.0005-0.0007 parts of folic acid, 0.00004-0.00006 parts of D-biotin, 0.000004-0.000006 parts of vitamin B12, 0.001-0.002 parts of anhydrous zinc acetate, 0.0005-0.0007 parts of anhydrous cobalt chloride and 0.0005-0.0007 parts of anhydrous sodium molybdate.

[0011] In addition, the present invention also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0012] S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the chloroform, and then drying to obtain a first mixed material;

[0013] S2, mixing the first mixed material, Webster salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly and part of water to obtain a second mixed material; mixing agar powder and the remaining water and dissolving them at 90-100° C. to obtain an agar solution;

[0014] S3. Mix the second mixed material and the agar solution to obtain the feed for adult Mylabris cichorii

[0015] In any embodiment, in step S2, the nutrient ethanol solution is prepared by the following steps: Dissolve dl-α-tocopheryl acetate, vitamin D3, and menadione in ethanol to obtain the nutrient ethanol solution.

[0016] In any embodiment, in step S2, the nutrient aqueous solution is prepared by the following steps: Dissolve nicotinamide, calcium D-pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, zinc acetate anhydrous, cobalt chloride anhydrous, and sodium molybdate anhydrous in water to obtain the nutrient aqueous solution.

[0017] In any embodiment, in step S2, the amount of water added to the second mixed material is 15% - 40% of the total weight of the water component added to the feed.

[0018] In any embodiment, in step S1, the nutrient ethanol solution or the nutrient aqueous solution is stored for standby at 3 - 5°C.

[0019] In any embodiment, in step S2, it further includes adding ascorbic acid and sorbic acid and mixing them with the first mixed material, Wesson salt, sucrose, L-cysteine, choline chloride, inositol, compound vitamin, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, apple sauce, royal jelly, and part of water to obtain the second mixed material.

[0020] Compared with the prior art, the beneficial effects of the present invention include: The feed for adult Mylabris cichorii proposed by the present invention optimizes the nutrient components, especially adding various vitamins, amino acids, and essential fatty acids. This feed can significantly extend the lifespan of adult Mylabris cichorii. The nutrients rich in the feed, such as vitamin D3, vitamin B group, cholesterol, and royal jelly, can significantly increase the egg-laying amount of adults. The feed formula covers various nutrient components such as protein, carbohydrate, vitamin, mineral, and essential fatty acid, which can meet the growth requirements of adult Mylabris cichorii, and ultimately can extend the lifespan of adults while increasing the egg-laying amount.

[0021] In addition, compared with the traditional feeding method that relies on natural ingredients (such as towel gourd flowers and locust eggs), the artificial feed proposed by the present invention reduces the time and manpower required for the planting, picking, and preservation of natural ingredients. All of the above provide strong support for the large-scale breeding of adult Mylabris cichorii and the stable production of cantharidin. Detailed Embodiments

[0022] The "range" disclosed in the present application is defined in the form of a lower limit and an upper limit, and a given range is defined by selecting a lower limit and an upper limit, and the selected lower limit and upper limit define the boundaries of the particular range. The range defined in this way can be inclusive or exclusive of the end values, and can be arbitrarily combined, that is, any lower limit can be combined with any upper limit to form a range. For example, if a range of 60 to 120 and 80 to 110 is listed for a particular parameter, it is understood that a range of 60 to 110 and 80 to 120 is also expected. In addition, if the minimum range values ​​1 and 2 are listed, and if the maximum range values ​​3, 4 and 5 are listed, the following ranges can all be expected: 1 to 3, 1 to 4, 1 to 5, 2 to 3, 2 to 4 and 2 to 5. In the present application, unless otherwise specified, the numerical range "a to b" represents an abbreviation of any real number combination between a and b, where a and b are both real numbers. For example, the numerical range "0-5" means that all real numbers between "0-5" are listed in this document, and "0-5" is just an abbreviation of these numerical combinations. In addition, when a parameter is expressed as an integer ≥ 2, it is equivalent to disclosing that the parameter is, for example, an integer of 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, etc.

[0023] If there is no special explanation, the "include" and "comprising" mentioned in this application are open-ended or closed-ended. For example, the "include" and "comprising" may mean that other components not listed may also be included or only the listed components may be included or only the listed components may be included.

[0024] If not specifically stated, in this application, the term "or" is inclusive. For example, the phrase "A or B" means "A, B, or both A and B". More specifically, any of the following conditions satisfies the condition "A or B": A is true (or exists) and B is false (or does not exist); A is false (or does not exist) and B is true (or exists); or both A and B are true (or exist).

[0025] The present specific embodiment provides a feed for adults of the eye-spotted meloides, which comprises, by weight, 11 to 15 parts of casein, 1 to 3 parts of Webster salt, 4 to 6 parts of sucrose, 9 to 12 parts of cellulose powder, 0.2 to 0.4 parts of L-cysteine, 0.2 to 0.3 parts of choline chloride, 0.08 to 0.1 parts of inositol, 0.325 to 0.4 parts of multivitamins, 2 to 3 parts of ethanol solution of nutrients, 0.10 to 0.13 parts of linoleic acid, 0.05 to 0.07 parts of linolenic acid, 12 to 14 parts of aqueous solution of nutrients, 1 to 2 parts of honey, 2 to 3 parts of agar powder, 0.03 to 0.04 parts of cholesterol, 33 to 67 parts of applesauce, 0.3 to 4 parts of royal jelly and 80 to 120 parts of water.

[0026] In some embodiments, it further includes 0.7 - 1 part of ascorbic acid and 0.2 - 0.4 part of sorbic acid. The added ascorbic acid and sorbic acid can extend the shelf life of the feed and ensure the stability of nutritional components.

[0027] In some embodiments, in the ethanol solution of nutritional components, it includes: 0.02 - 0.03 part of dl-α-tocopheryl acetate, 0.001 - 0.002 part of vitamin D3, and 0.001 - 0.002 part of menadione. The weight parts of each component in the ethanol solution of nutritional components are calculated relative to the total weight parts of the feed for adult Mylabris cichorii, and they are the weight parts in 2 - 3 parts of the ethanol solution of nutritional components. The sum of the weight parts of each component and the weight part of water in the ethanol solution of nutritional components is 2 - 3 parts.

[0028] In some embodiments, in the aqueous solution of nutritional components, it includes: 0.002 - 0.003 part of nicotinamide, 0.002 - 0.003 part of calcium D-pantothenate, 0.0005 - 0.0007 part of thiamine, 0.001 - 0.002 part of riboflavin, 0.0005 - 0.0007 part of pyridoxine, 0.0005 - 0.0007 part of folic acid, 0.00004 - 0.00006 part of D-biotin, 0.000004 - 0.000006 part of vitamin B12, 0.001 - 0.002 part of anhydrous zinc acetate, 0.0005 - 0.0007 part of anhydrous cobalt chloride, and 0.0005 - 0.0007 part of anhydrous sodium molybdate. The weight parts of each component in the aqueous solution of nutritional components are calculated relative to the total weight parts of the feed for adult Mylabris cichorii, and they are the weight parts in 12 - 14 parts of the aqueous solution of nutritional components. The sum of the weight parts of each component and the weight part of water in the aqueous solution of nutritional components is 12 - 14 parts.

[0029] This specific embodiment also provides a preparation method of the above-mentioned feed for adult Mylabris cichorii, including the following steps:

[0030] S1. Dissolve cholesterol in chloroform, then add casein and cellulose powder and mix them, and then dry to obtain the first mixed material;

[0031] S2. Mix the first mixed material, Weigh salt, sucrose, L-cysteine, choline chloride, inositol, compound vitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, apple sauce, royal jelly and part of water to obtain a second mixed material; mix agar powder and the remaining water and dissolve it at 90-100 °C to obtain an agar solution; the nutrient ethanol solution is prepared by the following steps: dissolve dl-α-tocopheryl acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; the nutrient aqueous solution is prepared by the following steps: dissolve niacinamide, calcium D-pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the amount of water added to the second mixed material is 15% to 40% of the total weight of the water component added to the feed, where the water component refers to 80 to 120 parts of the water component, and the sum of the water added to the second mixed material and the remaining water mixed with agar powder is 80 to 120 parts; the nutrient ethanol solution or the nutrient aqueous solution is stored for standby at 3-5 °C.

[0032] S3. Mix the second mixed material and the agar solution to obtain the feed for adult Mylabris cichorii.

[0033] In some embodiments, it further includes adding ascorbic acid and sorbic acid and mixing them with the first mixed material, Weigh salt, sucrose, L-cysteine, choline chloride, inositol, compound vitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, apple sauce, royal jelly and part of water to obtain the second mixed material.

[0034] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0035] In the present invention, terms such as "some embodiments", "this embodiment" and examples are involved, which describe subsets of all possible embodiments. However, it can be understood that "some embodiments" can be the same subsets or different subsets of all possible embodiments, and can be combined with each other without conflict.

[0036] If similar descriptions such as "first / second" appear in the application documents, the following explanations will be added. In the following descriptions, the terms "first\second\third" only distinguish similar objects and do not represent a specific order for the objects. It can be understood that "first\second\third" can be interchanged with a specific order or sequence when allowed, so that the embodiments described here can be implemented in an order other than that described here.

[0037] In this embodiment, the term "and / or" is merely a description of the association relationship between associated objects, indicating that three relationships may exist. For example, object A and / or object B may represent three situations: object A exists alone, object A and object B exist at the same time, and object B exists alone.

[0038] It should be noted that the multivitamin is called Vitamin mix, which is a single product and is purchased under the name: Vanderzant Vitamin Mix (for Insects) | Sigma-Aldrich.

[0039] Example 1

[0040] The present embodiment provides a feed for adults of the eye-spotted melaleuca, which comprises, by weight, 12 parts of casein, 2 parts of Wechsler salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins (i.e., Vitamin mix), 2 parts of nutrient ethanol solution, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of nutrient aqueous solution, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, 0.035 parts of cholesterol, 50 parts of applesauce, 2 parts of royal jelly, and 100 parts of water; the nutrient ethanol solution comprises: 0.02 parts of dl-α-tocopherol acetate, 0.03 parts of vitamin D3 0.001 part, and 0.001 part of menadione; the aqueous solution of nutrient ingredients includes: 0.002 part of niacinamide, 0.002 part of D-calcium pantothenate, 0.0005 part of thiamine, 0.001 part of riboflavin, 0.0005 part of pyridoxine, 0.0005 part of folic acid, 0.00004 part of D-biotin, 0.000004 part of vitamin B12, 0.001 part of anhydrous zinc acetate, 0.0005 part of anhydrous cobalt chloride, and 0.0005 part of anhydrous sodium molybdate.

[0041] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0042] S0, dissolving dl-α-tocopherol acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4°C for standby use;

[0043] S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the chloroform, and then drying to obtain a first mixed material;

[0044] S2, mixing the first mixed material, Webster salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly and 30 mL of water to obtain a second mixed material; mixing agar powder and the remaining 70 mL of water and dissolving them at 100° C. to obtain an agar solution;

[0045] S3, mixing the second mixed material and the agar solution to obtain the adult feed of the eye-spotted melaleuca.

[0046] Example 2

[0047] The present embodiment provides a feed for adults of the eye-spotted melaleuca, which comprises, by weight: 12 parts of casein, 2 parts of Wechsler salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of nutrient ethanol solution, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of nutrient aqueous solution, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, and 0.037 parts of cholesterol. parts, 60 parts of applesauce, 1 part of royal jelly and 90 parts of water; the nutrient ethanol solution includes: 0.02 parts of dl-α-tocopheryl acetate, 0.001 parts of vitamin D3, and 0.001 parts of menadione; the nutrient aqueous solution includes: 0.002 parts of niacinamide, 0.002 parts of D-calcium pantothenate, 0.0005 parts of thiamine, 0.001 parts of riboflavin, 0.0005 parts of pyridoxine, 0.0005 parts of folic acid, 0.00004 parts of D-biotin, 0.000004 parts of vitamin B12, 0.001 parts of anhydrous zinc acetate, 0.0005 parts of anhydrous cobalt chloride and 0.0005 parts of anhydrous sodium molybdate.

[0048] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0049] S0, dissolving dl-α-tocopherol acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4°C for standby use;

[0050] S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the chloroform, and then drying to obtain a first mixed material;

[0051] S2, mixing the first mixed material, Webster salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly and 20 mL of water to obtain a second mixed material; mixing agar powder and the remaining 70 mL of water and dissolving them at 90° C. to obtain an agar solution;

[0052] S3, mixing the second mixed material and the agar solution to obtain the adult feed of the eye-spotted melaleuca.

[0053] Example 3

[0054] The present embodiment provides a feed for adults of the eye-spotted melaleuca, which comprises, by weight: 12 parts of casein, 2 parts of Wechsler salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of ethanol solution of nutrients, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of aqueous solution of nutrients, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, and 0.033 parts of cholesterol. parts, 60 parts of applesauce, 3 parts of royal jelly and 90 parts of water; the nutrient component ethanol solution includes: 0.02 parts of dl-α-tocopheryl acetate, 0.001 parts of vitamin D3, and 0.001 parts of menadione; the nutrient component aqueous solution includes: 0.002 parts of niacinamide, 0.002 parts of D-calcium pantothenate, 0.0005 parts of thiamine, 0.001 parts of riboflavin, 0.0005 parts of pyridoxine, 0.0005 parts of folic acid, 0.00004 parts of D-biotin, 0.000004 parts of vitamin B12, 0.001 parts of anhydrous zinc acetate, 0.0005 parts of anhydrous cobalt chloride and 0.0005 parts of anhydrous sodium molybdate.

[0055] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0056] S0, dissolving dl-α-tocopherol acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4°C for standby use;

[0057] S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the chloroform, and then drying to obtain a first mixed material;

[0058] S2, the first mixed material, Wei's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly and 20 mL of water are mixed to obtain a second mixed material; agar powder and the remaining 70 mL of water are mixed and dissolved at 95° C. to obtain an agar solution; the amount of water added to the second mixed material is 40% of the total weight of the water component added to the feed;

[0059] S3, mixing the second mixed material and the agar solution to obtain the adult feed of the eye-spotted melaleuca.

[0060] Example 4

[0061] The present embodiment provides a feed for adults of the eye-spotted melaleuca, which comprises, by weight: 12 parts of casein, 2 parts of Wechsler salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of nutrient ethanol solution, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of nutrient aqueous solution, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, and 0.037 parts of cholesterol. parts, 40 parts of applesauce, 3 parts of royal jelly and 90 parts of water; the nutrient ethanol solution includes: 0.02 parts of dl-α-tocopheryl acetate, 0.001 parts of vitamin D3, and 0.001 parts of menadione; the nutrient aqueous solution includes: 0.002 parts of niacinamide, 0.002 parts of D-calcium pantothenate, 0.0005 parts of thiamine, 0.001 parts of riboflavin, 0.0005 parts of pyridoxine, 0.0005 parts of folic acid, 0.00004 parts of D-biotin, 0.000004 parts of vitamin B12, 0.001 parts of anhydrous zinc acetate, 0.0005 parts of anhydrous cobalt chloride and 0.0005 parts of anhydrous sodium molybdate.

[0062] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0063] S0, dissolving dl-α-tocopherol acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4°C for standby use;

[0064] S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the chloroform, and then drying to obtain a first mixed material;

[0065] S2, the first mixed material, Wei's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly and 20 mL of water are mixed to obtain a second mixed material; agar powder and the remaining 70 mL of water are mixed and dissolved at 95° C. to obtain an agar solution; the amount of water added to the second mixed material is 25% of the total weight of the water component added to the feed;

[0066] S3, mixing the second mixed material and the agar solution to obtain the adult feed of the eye-spotted melaleuca.

[0067] Example 5

[0068] The present embodiment provides a feed for adults of the eye-spotted melaleuca, which comprises, by weight: 12 parts of casein, 2 parts of Wechsler salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of nutrient ethanol solution, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of nutrient aqueous solution, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, 0.0316364 parts of cholesterol, 50 parts of applesauce, 2 parts of royal jelly and 100 parts of water; the nutrient ethanol solution comprises: 0.02 parts of dl-α-tocopherol acetate, 0.03 parts of vitamin D3 0.001 part, and 0.001 part of menadione; the aqueous solution of nutrient ingredients includes: 0.002 part of niacinamide, 0.002 part of D-calcium pantothenate, 0.0005 part of thiamine, 0.001 part of riboflavin, 0.0005 part of pyridoxine, 0.0005 part of folic acid, 0.00004 part of D-biotin, 0.000004 part of vitamin B12, 0.001 part of anhydrous zinc acetate, 0.0005 part of anhydrous cobalt chloride, and 0.0005 part of anhydrous sodium molybdate.

[0069] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0070] S0, dissolving dl-α-tocopherol acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4°C for standby use;

[0071] S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the chloroform, and then drying to obtain a first mixed material;

[0072] S2, the first mixed material, Wei's salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly and 30 mL of water are mixed to obtain a second mixed material; agar powder and the remaining 70 ml of water are mixed and dissolved at 95° C. to obtain an agar solution; the amount of water added to the second mixed material is 25% of the total weight of the water component added to the feed;

[0073] S3, mixing the second mixed material and the agar solution to obtain the adult feed of the eye-spotted melaleuca.

[0074] Example 6

[0075] The present embodiment provides a feed for adults of the eye-spotted melaleuca, which comprises, by weight: 12 parts of casein, 2 parts of Wechsler salt, 4 parts of sucrose, 10 parts of cellulose powder, 0.2 parts of L-cysteine, 0.2 parts of choline chloride, 0.08 parts of inositol, 0.325 parts of multivitamins, 2 parts of nutrient ethanol solution, 0.11 parts of linoleic acid, 0.054 parts of linolenic acid, 12 parts of nutrient aqueous solution, 0.8 parts of ascorbic acid, 0.2 parts of sorbic acid, 1 part of honey, 2 parts of agar powder, 0.0383636 parts of cholesterol, 50 parts of applesauce, 2 parts of royal jelly and 100 parts of water; the nutrient ethanol solution comprises: 0.02 parts of dl-α-tocopherol acetate, 0.03 parts of vitamin D3 0.001 part, and 0.001 part of menadione; the aqueous solution of nutrient ingredients includes: 0.002 part of niacinamide, 0.002 part of D-calcium pantothenate, 0.0005 part of thiamine, 0.001 part of riboflavin, 0.0005 part of pyridoxine, 0.0005 part of folic acid, 0.00004 part of D-biotin, 0.000004 part of vitamin B12, 0.001 part of anhydrous zinc acetate, 0.0005 part of anhydrous cobalt chloride, and 0.0005 part of anhydrous sodium molybdate.

[0076] This embodiment also provides a method for preparing the above-mentioned adult feed of Meloidogyne ocellaris, comprising the following steps:

[0077] S0, dissolving dl-α-tocopherol acetate, vitamin D3 and menadione in ethanol to obtain the nutrient ethanol solution; dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient aqueous solution; the nutrient ethanol solution or the nutrient aqueous solution is stored at 4°C for standby use;

[0078] S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the chloroform, and then drying to obtain a first mixed material;

[0079] S2. Mix the first mixed material, Weigh salt, sucrose, L-cysteine, choline chloride, inositol, compound vitamins, ethanol solution of nutrients, linoleic acid, linolenic acid, aqueous solution of nutrients, honey, apple sauce, royal jelly and 30 mL of water to obtain a second mixed material; mix agar powder and the remaining 70 mL of water and dissolve at 95 °C to obtain an agar solution; the amount of water added to the second mixed material is 25% of the total weight of the water component added to the feed.

[0080] S3. Mix the second mixed material and the agar solution to obtain the adult feed of Mylabris cichorii.

[0081] Comparative Example 1

[0082] The difference between the adult feed of Mylabris cichorii in this comparative example and that in the example is that the natural artificial feed is used.

[0083] The natural artificial feed contains the following components: 100 ml of water, 36 g of soybean powder, 1 g of honey, 1 g of royal jelly, 1.6 g of agar powder.

[0084] The preparation method of the adult feed of Mylabris cichorii in this comparative example is as follows:

[0085] S0. Sterilize the soybean powder at 121 °C for 30 min to obtain a sterilized material.

[0086] S1. Mix the sterilized material obtained in step S0 with royal jelly, honey and 50 ml of water to obtain a mixed material.

[0087] S2. Dissolve agar powder and the remaining water at 100 °C, stir the obtained mixed solution with the mixed material in step S1, and cool to obtain an artificial feed.

[0088] Related tests

[0089] Divide 35 adult Mylabris cichorii, 35 males and 35 females, into 7 groups, Examples 1 - 6 and Comparative Example 1, with 5 males and 5 females in each group; group them and raise them in 7 transparent containers in an incubator at a constant temperature of 30 °C and under a light condition of 16 h. The containers are filled with sand and soil with a sand-to-soil ratio of 7:3, and the feed is replaced at 10:00 am every day. The average daily food intake per individual, average adult lifespan, pre-oviposition period, egg production, and average number of eggs per individual in Examples 1 - 6 and Comparative Example 1 are shown in Table 1.

[0090] Table 1

[0091]

[0092] As can be seen from Table 1, the average daily food intake of the single-headed adults in the examples and the comparative examples is not much different. However, the average lifespan, egg production, and average number of eggs of the adult Mylabris cichorii fed with the feed in Examples 1-6 are relatively high, and the pre-oviposition period is relatively short. In contrast, the average lifespan, egg production, and average number of eggs of the adult Mylabris cichorii fed with the feed in the comparative example are relatively low, and the pre-oviposition period is long. It can be seen that the feed in Examples 1-6 can significantly increase the average lifespan, egg production, and average number of eggs of the adult Mylabris cichorii, and can shorten the time of the pre-oviposition period.

[0093] The specific implementation manners of the present invention described above do not constitute a limitation to the protection scope of the present invention. Any other corresponding changes and deformations made according to the technical concept of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A feed for adults of Meloidogyne ocellaris, characterized in that: Calculated by weight, it includes: 11-15 parts of casein, 1-3 parts of Webster salt, 4-6 parts of sucrose, 9-12 parts of cellulose powder, 0.2-0.4 parts of L-cysteine, 0.2-0.3 parts of choline chloride, 0.08-0.1 parts of inositol, 0.325-0.4 parts of multivitamins, 2-3 parts of ethanol solution of nutrients, 0.10-0.13 parts of linoleic acid, 0.05-0.07 parts of linolenic acid, 12-14 parts of aqueous solution of nutrients, 1-2 parts of honey, 2-3 parts of agar powder, 0.03-0.04 parts of cholesterol, 33-67 parts of applesauce, 0.3-4 parts of royal jelly and 80-120 parts of water.

2. The feed for adults of Meloidogyne ocellaris according to claim 1, characterized in that: It also includes 0.7-1 part of ascorbic acid and 0.2-0.4 part of sorbic acid.

3. The feed for adults of Meloidogyne ocellaris according to claim 1, characterized in that: The nutrient component ethanol solution comprises: 0.01-0.03 parts of dl-α-tocopherol acetate, 0.001-0.002 parts of vitamin D3, and 0.001-0.002 parts of menadione.

4. The feed for adults of Meloidogyne ocellaris according to claim 1, characterized in that: The aqueous solution of nutrient components includes: 0.002-0.003 parts of nicotinamide, 0.002-0.003 parts of D-calcium pantothenate, 0.0005-0.0007 parts of thiamine, 0.001-0.002 parts of riboflavin, 0.0005-0.0007 parts of pyridoxine, 0.0005-0.0007 parts of folic acid, 0.00004-0.00006 parts of D-biotin, 0.000004-0.000006 parts of vitamin B12, 0.001-0.002 parts of anhydrous zinc acetate, 0.0005-0.0007 parts of anhydrous cobalt chloride and 0.0005-0.0007 parts of anhydrous sodium molybdate.

5. A method for preparing a feed for adults of Meloidogyne ocellaris according to any one of claims 1 to 4, characterized in that: The following steps are involved: S1, dissolving cholesterol in chloroform, then adding casein and cellulose powder to mix with the chloroform, and then drying to obtain a first mixed material; S2, mixing the first mixed material, Webster salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, nutrient ethanol solution, linoleic acid, linolenic acid, nutrient aqueous solution, honey, applesauce, royal jelly and part of water to obtain a second mixed material; mixing agar powder and the remaining water and dissolving them at 90-100° C. to obtain an agar solution; S3, mixing the second mixed material and the agar solution to obtain the adult feed of the eye-spotted melaleuca.

6. The method for preparing the feed for adults of Meloidogyne ocellaris according to claim 5, characterized in that: In step S2, the nutrient component ethanol solution is prepared by the following steps: dissolving dl-α-tocopherol acetate, vitamin D3 and menadione in ethanol to obtain the nutrient component ethanol solution.

7. The method for preparing the feed for adults of Meloidogyne ocellaris according to claim 5, characterized in that: In step S2, the nutrient solution is prepared by the following steps: dissolving nicotinamide, D-calcium pantothenate, thiamine, riboflavin, pyridoxine, folic acid, D-biotin, vitamin B12, anhydrous zinc acetate, anhydrous cobalt chloride and anhydrous sodium molybdate in water to obtain the nutrient solution.

8. The method for preparing feed for adults of Meloidogyne ocellaris according to claim 5, characterized in that: In step S2, the amount of water added to the second mixed material is 15% to 40% of the total weight of the water component added to the feed.

9. The method for preparing the feed for adults of Meloidogyne ocellaris according to claim 5, characterized in that: In step S1, the nutrient component ethanol solution or the nutrient component aqueous solution is stored at 3-5°C for later use.

10. The method for preparing feed for adults of Meloidogyne ocellaris according to claim 5, characterized in that: In step S2, it also includes adding ascorbic acid and sorbic acid to the first mixed material, Webster salt, sucrose, L-cysteine, choline chloride, inositol, multivitamins, ethanol solution of nutrients, linoleic acid, linolenic acid, aqueous solution of nutrients, honey, applesauce, royal jelly and part of water to obtain the second mixed material.

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