Method for preparing algae essence by using enzymatic hydrolysis method

A technology of seaweed and enzymatic hydrolysis, applied in the direction of chemicals, chemical instruments and methods, botany equipment and methods for biological control, etc., can solve the problems of easy decomposition and damage, unstable active ingredients, and reduced content of active ingredients in seaweed essence And other issues

Active Publication Date: 2009-11-18
BEIJING LEILI MARINE BIOINDUSTRY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, these active ingredients in seaweed are unstable, especially some natural plant growth regulating substances, which are easily decomposed and destroyed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Feeding:

[0014] Dried kelp 578.4Kg

[0015] Water 5784Kg (1.3 meters from the bottom of the tank)

[0016] operate:

[0017] 1. Put water into the tank first, then add dried kelp crushed to 200 mesh, soak for 2-5 hours, and then process it into a slurry with a colloid mill.

[0018] 2. Then degrade, add cellulase 1000-4000μ / Kg digestion solution and alginate lyase 500-1500μ / Kg digestion solution (if the digestion solution does not meet the optimum pH value, adjust the pH value with acid or alkali first) in Enzymolysis at 30-60°C for 2-6 hours, take a sample 15 minutes before the end of the enzymolysis to measure the viscosity, if the temperature is lower than 70 seconds at 30°C, it is qualified (Note: the viscometer model is "0.7∽0.8", the viscosity of water is 41 seconds), The enzyme was then inactivated at a temperature of 95°C for 15 minutes. Then open the cooling water to lower the temperature, and then centrifuge to remove the residue.

[0019] 3. Concentrat...

Embodiment 2

[0022] Feeding:

[0023] Dried Sargassum 578.4Kg

[0024] Water 5784Kg (1.3 meters from the bottom of the tank)

[0025] operate:

[0026] 1. Put water into the tank first, then add dried sargassum crushed to 200 mesh, soak for 2-5 hours, and then process it into a slurry with a colloid mill.

[0027] 2. Then degrade, add cellulase 1000-4000μ / Kg digestion solution and alginate lyase 500-1500μ / Kg digestion solution (if the digestion solution does not meet the optimum pH value, adjust the pH value with acid or alkali first) in the Enzymolysis at 30-60°C for 2-6 hours, take a sample 15 minutes before the end of the enzymolysis to measure the viscosity, if the temperature is lower than 70 seconds at 30°C, it is qualified (Note: the viscometer model is "0.7∽0.8", the viscosity of water is 41 seconds), The enzyme was then inactivated at a temperature of 95°C for 15 minutes. Then open the cooling water to lower the temperature, and centrifuge to remove the residue.

[0028] 3. T...

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PUM

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Abstract

The invention improves the extraction yield of algae essence by using cellulase for decomposing cellulose in algae after dry algae which is ground and desanded is soaked in water and subjected to colloid mill treatment, and then leads alginic acid with large molecular weight to be degraded to alginic acid with small molecular weight by using alginate lyase, thus leading effective compositions in algae to be less damaged and having high extraction yield.

Description

technical field [0001] The invention relates to a method for preparing seaweed essence by enzymatic hydrolysis. Background technique [0002] Seaweed (kelp, sargassum and other brown algae) not only contains macroelements, trace elements and various minerals required by plants, but also contains active ingredients such as alginic acid and natural plant growth regulators that are rare in land plants, which have the function of promoting Plant growth, enhanced plant disease resistance and other functions. [0003] However, these active ingredients in seaweed are unstable, especially some natural plant growth regulating substances, which are easily decomposed and destroyed after being digested and degraded under strong acidic and strong alkaline conditions, resulting in a decrease in the content of active ingredients in seaweed essence. [0004] The preparation of seaweed essence for fertilizer by enzymatic hydrolysis can be digested and degraded under moderate acid-base condi...

Claims

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Application Information

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IPC IPC(8): C12P1/00C05F11/00A01N65/03A01P21/00B09C1/08C09K17/00
Inventor 汤洁
Owner BEIJING LEILI MARINE BIOINDUSTRY INC
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