Method for rapid extraction and purification of epigallocatechin gallate from green tea scraps

A technology of epigallocatechin and gallate, which is applied in the field of pharmaceutical applications, can solve the problems of complex steps, high solvent toxicity, and low product yield, and achieve the effect of product safety and easy operation

Active Publication Date: 2015-10-28
SUZHOU VOCATIONAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The technical problem mainly solved by the present invention is to provide a method that can make full use of the leftovers of green tea to quickly extract, separate and purify epigallocatechin gallate, so that the leftovers of green tea can be recycled as waste, and at the same time solve the problem of In the purification process of EGCG, the steps are complicated, time-consuming, the solvent used is highly toxic, the product yield is low, and the industrialization cannot be realized.

Method used

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  • Method for rapid extraction and purification of epigallocatechin gallate from green tea scraps
  • Method for rapid extraction and purification of epigallocatechin gallate from green tea scraps

Examples

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Embodiment 1

[0033] Green tea waste 1 kg, add 8L of 60% ethanol to extract by ultrasonic vibration, extract twice, each time for 30 minutes, and combine the extracts; extract the extracts below 80°C to recover ethanol under reduced pressure until there is no alcohol smell, and concentrate to a relative density of 1.20 to 1.22 Clear the cream; cool it, and adsorb it on a polyamide resin column (the amount of resin used is 10 times the weight of the cream, and the diameter-to-height ratio of the column is 1:20), elute with 95% ethanol: 36% acetic acid (volume ratio is 5:1) , thin-layer chromatography tracking detection, collecting epigallocatechin gallate (EGCG) fractions, decolorized with 2% (g / 100ml) activated carbon, filtered, and the filtrate was reduced at low temperature under the protection of nitrogen at 60°C-80°C. Concentrate under pressure to a relative density of 1.15-1.18, freeze-dry. Recrystallized with ethanol to obtain epigallocatechin gallate (EGCG) with a purity of 98.3% and...

Embodiment 2

[0035] Add 1 kg of green tea leftovers, add 12L of 60% ethanol to extract by ultrasonic vibration, extract twice, each time for 30min, and combine the extracts. The extract is recovered under reduced pressure below 80°C until there is no alcohol smell, and concentrated to a clear paste with a relative density of 1.20 to 1.22. Cool, adsorb on a polyamide resin column (the amount of resin used is 15 times the paste weight, and the diameter-to-height ratio of the column is 1:20), elute with 95% ethanol: 36% acetic acid (volume ratio: 2:1), thin layer Chromatography tracking detection, collecting epigallocatechin gallate (EGCG) fractions, adsorbing with 2.5% (g / 100ml) activated carbon, filtering, and concentrating the filtrate under reduced pressure at 60°C-80°C under nitrogen protection at low temperature to The relative density is 1.15-1.18, freeze-dried. Recrystallized with ethanol to obtain epigallocatechin gallate (EGCG) with a purity of 98.6% and a yield of 12%.

Embodiment 3

[0037] Add 1 kg of green tea waste, add 16L of 60% ethanol to extract by ultrasonic vibration, extract twice, each time for 30 minutes, and combine the extracts. The extract is recovered under reduced pressure below 80°C until there is no alcohol smell, and concentrated to a clear paste with a relative density of 1.20 to 1.22. Cool, adsorb on a polyamide resin column (the amount of resin used is 20 times the paste weight, and the ratio of diameter to height of the column is 1:20), elute with 95% ethanol: 36% acetic acid (volume ratio is 1:1), thin layer Chromatography tracking detection, collecting epigallocatechin gallate (EGCG) fractions, adsorbing with 3% (g / 100ml) activated carbon, filtering, and concentrating the filtrate under reduced pressure at 60°C-80°C under nitrogen protection at low temperature to The relative density is 1.15-1.18, freeze-dried. Recrystallized with ethanol to obtain epigallocatechin gallate (EGCG) with a purity of 98.9% and a yield of 16%.

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Abstract

The invention relates to a method for rapidly extracting, separating and purifying epigallocatechin gallate from leftover of green tea, which comprises the following steps: 1)extracting the leftover of the green tea by ethanol through a conventional method; 2)performing pressure reduction on an extract below the temperature of 80 DEG C and recovering ethanol until no alcohol taste; 3)absorbing an alcohol extract by polyamide resin and separating; 4)collecting epigallocatechin gallate fraction, decolouring by active carbon, performing pressure reduction at low temperature and concentrating; and 5)recrystallizing by ethanol to obtain the high purity epigallocatechin gallate. According to the invention, the method is simple to operate, only one resin is employed to reach one-step completion of adsorption, separation and purification, the used solvent has the advantages of no toxicity, safety and reliability, and the product has the advantages of high purity and low cost, and is suitable for industrial production.

Description

Technical field [0001] The invention involves the field of medical applications, especially the method of quickly extracting the separation of separation and purifying meals from green tea. Background technique [0002] The polyphenols in tea account for 18%-36%of the dry weight of the tea, including euritol, flavonoids, flavonoids and flavonol, anthocyanin and leuntein, and phenolic acid and phenolic acidEssenceAmong them, the catexin compound is the main component of the tea polyphenol, accounting for 12%-24%of the dry weight of the tea, and 70%-80%of the total number of tea polyphenols.Modern medical research has found that tea polyphenol is a new type of natural antioxidant that has the physiological effects such as anti -tumor, anti -aging, anti -mutation, inhibiting tumor growth, weight loss and anti -pressure, and preventive cardiovascular disease.And these physiological functions are mainly manifested through the catechins in it. Among them, the osteopais in the ester typ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 刘臣周莹杨琴于世翔
Owner SUZHOU VOCATIONAL UNIV
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