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Technology of animal high-calcium powder, chondrine and collagen by composite enzyme method

A technology of collagen and production technology, which is applied in the production field of extracting high calcium powder, chondroitin and collagen from animal cartilage by biological fermentation method, can solve the problems of wasting animal cartilage protein resources and environmental pollution, and achieve high promotion Use value, effect of acid-base consumption decrease

Inactive Publication Date: 2007-01-17
郭秀明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing process of extracting chondroitin is to destroy the protein with strong alkali and high temperature, and then separate the chondroitin by enzymatic hydrolysis. The destroyed protein is discharged with the waste water, which not only wastes the precious protein resources in animal cartilage, but also causes severe environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Simply wash 1 ton of fresh bovine cartilage (including costal cartilage and throat) and break it into pieces of 5-20mm, mix it with water at a ratio of 1:1, add it to the enamel reaction kettle, heat it to 100°C and keep it for 1-2 hours, ensure that the bones are cooked to a certain degree, then pass cold water into the reactor jacket to cool down, when the temperature drops to 40-50°C, add 1 kg of collagenase-based compound enzyme, keep the temperature at 40-50°C, stir and hydrolyze After 8 hours, coarsely filter out the bone dregs. After the bone dregs are washed, dried and crushed, about 200 kg of high calcium powder is obtained. The coarsely filtered liquid is then finely filtered to obtain a clear fine filtrate, which is added with more than 90% alcohol to an alcohol concentration of 60-70%, and the chondroitin crystals are precipitated. The liquid is a mixed solution of collagen and alcohol, and the chondroitin crystals are washed with more than 90% alcohol and t...

Embodiment 2

[0012] Crush 1 kg of dried shark skulls into 5-20mm pieces, add water at a ratio of 1:4, heat to 100°C, keep for 1-2 hours, then cool down to 40-50°C, add collagenase as the main Keep the temperature of 40-50 ℃, stir and hydrolyze for 8 hours, and filter out the bone residue with 100 mesh. After the bone residue is washed, dried and crushed, about 300 grams of high calcium powder can be obtained; Fine filtration to obtain a clear fine filtrate, add more than 90% alcohol to this solution until the alcohol concentration is 60-70%, the chondroitin crystals precipitate out, the liquid is a mixed solution of collagen and alcohol, the chondroitin crystals are washed and dried with more than 90% alcohol Dried to obtain 120 grams of chondroitin powder; the mixed solution was distilled to recover alcohol to become a collagen solution, concentrated to a concentration of 70%, to obtain 300 grams of honey-like sticky paste.

Embodiment 3

[0014] The steps and methods are the same as in Examples 1 and 2, except that the dried pig nose bone is used as a raw material, and the pig nose bone contains about 40% chondroitin, which can be extracted with this technology, and the yield can reach 35% (the finished product has a purity of 90% as detected by the CPC method). %), which proves that the technology co-produces chondroitin while producing collagen, and its yield, product purity and appearance can all reach or exceed the level of single production of chondroitin.

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PUM

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Abstract

The present invention relates to a biological fermentative production technology of extracting high-calcium powder, chondroitin sulfate and collagen from animal cartilage which includes the following steps: the animal cartilage is simply broken into 5-20mm fragments, mixed with 1-4 times weight of water in the reaction kettle, heated to 100DEG C and kept for 1-2 hours to denature the proteins, then cold water is passed over into the kettle jacket to cool down the kettle to 40-50DEG C, compound enzyme in mass ratio of 1:0.001 which is mainly collagenase is then added, the mixture is stirred and hydrolyzed for 8-12 hours under the condition of 40-50DEG C, pH 7-8, bone residue that contains calcium phosphate is obtained after simple filtration through 100-meshed sieves; the filtrate is clarificated after fine filtration with filter press, then ethanol is added to the final concentration of 60-70%, chondroitin will be sedimentated, while remaining liquid is the mixture of collagen and ethanol. The sedimentated calcium phosphate is washed and dried and becomes calcium phosphate powder. The crystal sediment of chondroitin is purified by ethanol-washing. Collagen is separated after the mixture of collagen and ethanol goes through the ethanol regenerating column. This technology can produce different products with high purity from animal cartilage and solves the problems of pollution and single product of traditional technology.

Description

technical field [0001] The invention relates to a processing technology for animal cartilage, in particular to a production technology for extracting high-calcium powder, chondroitin and collagen from animal cartilage by biological fermentation. Background technique [0002] Animal cartilage, including cartilage of terrestrial animals and aquatic animals, is now mainly used to extract chondroitin, while animal cartilage contains chondroitin, and also contains about 5-10 times as much protein as chondroitin, of which more than 90% is collagen protein. The existing process of extracting chondroitin is to destroy the protein with strong alkali and high temperature, and then separate the chondroitin by enzymatic hydrolysis. The destroyed protein is discharged with the waste water, which not only wastes the precious protein resources in animal cartilage, but also causes Serious environmental pollution. Contents of the invention [0003] The purpose of the present invention is...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12P21/02C12P3/00
Inventor 郭秀明
Owner 郭秀明
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