Composite bone tissue engineering rack material and its prepn

A technology of bone tissue engineering and scaffold materials, applied in medical science, prosthesis, etc., can solve the problems of damaged cells, insufficient strength of polymer materials, and fragility

Inactive Publication Date: 2004-09-08
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that these materials are not ideal. The strength of polymer materials is insufficient, and they are easily deformed under stress, which will damage the transplanted cells, while bioceramics degrade slowly and easily break under stress.

Method used

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  • Composite bone tissue engineering rack material and its prepn
  • Composite bone tissue engineering rack material and its prepn

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] With 0.3% malonic acid solution as the swelling solution, a collagen swelling solution with a solid content of 0.5% was prepared. Weigh 60 g of collagen swelling solution with a solid content of 0.5% and 0.3 g of hydroxyapatite, and mix them uniformly to obtain a collagen-HA dispersion. Pre-freeze at -30°C for at least 4 hours, then freeze-dry at -30°C for 15 hours, -15°C for 18 hours, 0°C for 15 hours, 10°C for 7 hours, and 20°C for 4 hours to obtain uncrosslinked collagen-HA scaffold materials.

[0019] At room temperature, soak the prepared uncrosslinked collagen-HA scaffold material in 40% ethanol containing 120ml50mmol / L2-morpholineethanesulfonic acid (MES) for 30min, then soak the collagen-HA scaffold material in 120ml crosslinked Solution (containing 50mmol / L MES, 24mmol / L carbodiimide (EDC), 5mmol / L succinimide (NHS), 0.2% chondroitin sulfate) cross-linked at room temperature for 4h. Put the scaffold in 0.1mol / L Na 2 HPO 4 Wash in water for 1 hour, wash in 1m...

Embodiment 2

[0029]With 0.3% malonic acid solution as the swelling solution, a collagen swelling solution with a solid content of 0.5% was prepared. Weigh 60 grams of collagen swelling solution with a solid content of 0.5% and 1.2 grams of hydroxyapatite, and mix them uniformly to obtain a collagen-HA dispersion. Pre-freeze first, and then freeze-dry to obtain uncrosslinked collagen-HA scaffold material.

[0030] At room temperature, soak the prepared uncrosslinked collagen-HA scaffold material in 40% ethanol containing 120ml50mmol / L2-morpholineethanesulfonic acid (MES) for 30min, then soak the collagen-HA scaffold material in 120ml crosslinked Solution (containing 50mmol / L MES, 24mmol / L carbodiimide (EDC), 5mmol / L succinimide (NHS), 0.5% chondroitin sulfate) cross-linked at room temperature for 4h. Put the scaffold in 0.1mol / L Na 2 HPO 4 Wash for 1 hour in 1 mol / L NaCl, 2 hours in 1 mol / L NaCl, 1 day and night in 2 mol / L NaCl, and finally, wash with water to remove residual cross-linki...

Embodiment 3

[0033] With 0.3% malonic acid solution as the swelling solution, a collagen swelling solution with a solid content of 0.7% was prepared. Weigh 60 grams of collagen swelling solution with a solid content of 0.7% and 0.84 grams of hydroxyapatite, and mix them uniformly to obtain a collagen-HA dispersion. Pre-freeze first, and then freeze-dry to obtain uncrosslinked collagen-HA scaffold material.

[0034] At room temperature, soak the prepared uncrosslinked collagen-HA scaffold material in 40% ethanol containing 168ml50mmol / L2-morpholineethanesulfonic acid (MES) for 30min, then soak the collagen-HA scaffold material in 168ml crosslinked Solution (containing 50mmol / L MES, 24mmol / L carbodiimide (EDC), 5mmol / L succinimide (NHS), 0.2% chondroitin sulfate) cross-linked at room temperature for 4h. Put the scaffold in 0.1mol / L Na 2 HPO 4 Wash for 1 hour in 1 mol / L NaCl, 2 hours in 1 mol / L NaCl, 1 day and night in 2 mol / L NaCl, and finally, wash with water to remove residual cross-lin...

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Abstract

The composite bone tissue engineering rack material includes collagen, hydroxyapatite, chondroitin sulfate and bone morphogenetic protein; and has 3D porous structure of aperture 50-150 microns. The ratio between collagen and hydroxyapatite is 1 to 1-10, and the chondroitin sulfate is compounded with other components via cross-linking. The material may be constituted into tissue engineering artificial bone based on tissue engineering principle. The present invention has excellent physical, chemical and biological performance, is excellent bone tissue engineering rack material, and may be used in repairing bone defect caused by different reasons clinically.

Description

technical field [0001] The invention relates to a bone tissue engineering scaffold material, in particular to a collagen-hydroxyapatite (HA)-chondroitin sulfate (CS)-bone morphogenetic protein (BMP) composite bone tissue engineering scaffold material and a preparation method thereof. Applying the principle of tissue engineering, the material can be used as a scaffold material to construct tissue-engineered artificial bone, which can be used to repair bone defects caused by various reasons. Background technique [0002] The incidence of bone defects caused by trauma, tumor, infection and other causes is high, which poses a great threat to human health. At present, the repair of bone defects mainly uses autologous bone, allogeneic bone graft materials, and artificial bones such as metals, polymer compounds, natural or synthetic calcium phosphate materials such as coral, and hydroxyapatite. These treatments are limited due to limited donors, immune responses, and the risk of i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/46C08L89/00
Inventor 张其清刘玲蓉李学敏
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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