Method for separating chondroitin polysulfate from heparin sodium by extraction method

A technology of chondroitin polysulfate and sodium heparin, applied in the directions of liquid solution solvent extraction, fermentation, etc., can solve problems such as the separation technology of chondroitin polysulfate that is not reported, and achieve the effect of promoting the production of live pigs

Active Publication Date: 2009-11-11
QINGDAO JIULONG BIO PHARMA
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

On April 17-18 and April 24-25, an international symposium on heparin sodium was held in the United States. On May 4, the official website of the US FDA announced that the main cause of death was polychondroitin sulfate. On the 7th, CCTV reported that there had been more than 80 deaths in the United States, and so far there has been no report on the technology for separating chondroitin polysulfate from heparin sodium

Method used

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  • Method for separating chondroitin polysulfate from heparin sodium by extraction method
  • Method for separating chondroitin polysulfate from heparin sodium by extraction method
  • Method for separating chondroitin polysulfate from heparin sodium by extraction method

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Experimental program
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Embodiment 1

[0040] 1. Extraction

[0041] 1. Crude product dissolution:

[0042] Open the water valve, add 900L of drinking water, then add 15 billion units of heparin sodium crude product into the reaction tank, add while stirring, after stirring for 6 hours, use a stirring rod to test whether the bottom is completely dissolved;

[0043] 2. Enzyme hydrolysis:

[0044] Adjust the pH of the solution in the previous step to 7.5 with hydrochloric acid solution, add 1500 g of pepsin when the temperature rises to 53 °C, then add 3000 g of trypsin, and keep warm at 53 °C for 3 hours;

[0045] 3. Rapid heating:

[0046] Heat the enzymolysis solution from the previous step to 88°C within 35 minutes, stand still for 15 minutes, start stirring, pass in circulating water to cool down, and when the temperature drops to 50°C, adjust the pH to 11.0 with 6mol / L sodium hydroxide solution, stand still Stratification for 24 hours;

[0047] 4. Centrifugation of impurities precipitated at the bottom:

...

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Abstract

The invention discloses a method for separating chondroitin polysulfate from heparin sodium by an extraction method, breaking through the conclusion drawn by domestic and international experts, i.e., the chondroitin polysulfate in the heparin sodium is inseparable. The technical scheme is as follows: an acetone extraction method is adopted to remove the chondroitin polysulfate in the heparin sodium and thoroughly separate the chondroitin polysulfate from the heparin sodium by the refinement steps of crude product dissolution, enzymolysis, rapid heating, impurity centrifugation, a plurality of precipitation and oxidation so that the refined product rate of the heparin sodium reaches over 83 percent, the activity valence is over 180 usp/mg and over 200 iu/mg by being converted into the WHO international standard, and the quality standard accords with various indexes specified by Chinese pharmacopoeias, America pharmacopoeias, English pharmacopoeias and Western European pharmacopoeias.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for separating chondroitin polysulfate from heparin sodium. Background technique [0002] Heparin sodium is a kind of biochemical drug extracted from pig small intestine mucosa, which is an irreplaceable, life-saving drug in surgery and an unstoppable drug in the market. Since it was used clinically in the 1940s, its scope of application has continued to expand, especially since the 1990s, the product is mainly used clinically to prevent thrombosis and treat cardiovascular diseases, blood diseases, uremia, etc. Western countries have begun to study the preventive effect of heparin sodium on cancer, and its new uses are increasing. [0003] Since the 1970s, the production process of heparin sodium in my country has been continuously improved, and China has become the country with the largest output of heparin sodium in the world. More than 70% of heparin sodium products in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/10C08B37/08B01D11/04C12P19/04
Inventor 曲士业刘乃山迟培升
Owner QINGDAO JIULONG BIO PHARMA
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