Composition for cultivating dwarfed cyclocarya paliurus seedlings and method and application thereof

Abstract

The invention belongs to the field of plant maintenance, and particularly relates to a composition for cultivating dwarf cyclocarya paliurus seedlings and a method and application thereof. The composition is composed of a triiodobenzoic acid solution, a phosphate fertilizer and a potash fertilizer, triiodobenzoic acid can reduce the height of cyclocarya paliurus seedlings, increase of the ground diameter of the seedlings is promoted, dwarfing of the cyclocarya paliurus seedlings is facilitated, and the phosphate fertilizer and the potash fertilizer can adjust the leaf length, the leaf width and the leaf thickness of the cyclocarya paliurus seedlings. The cyclocarya paliurus seedlings can be better dwarfed by combining the scientific proportion with the composition, and theoretical and technical support is provided for promoting large-area popularization of cyclocarya paliurus artificial economic forests.

Description

Technical Field

[0001] The present invention belongs to the field of plant cultivation, and more specifically, relates to a composition for cultivating dwarf Cyclocarya paliurus seedlings, and its method and application. Background Art

[0002] Cyclocarya paliurus is a tall arbor of the genus Cyclocarya in the Juglandaceae family, a rare and endemic plant in China. It is distributed south of the Yangtze River, with scattered communities and scarce quantity, and the national wild resources are few. The main distribution areas include Hunan, Jiangxi, Hubei, Jiangxi, Guangxi, etc. Cyclocarya paliurus is a national key protected endangered plant, and its leaves contain a variety of bioactive substances, with medicinal values such as lowering blood sugar, blood pressure, blood lipid and prolonging life. In recent years, researchers have conducted a large number of studies on the efficacy of Cyclocarya paliurus in biological medicine and food health care, and launched many related health food and medical products. The medicinal value of Cyclocarya paliurus has been gradually recognized and promoted by people, and the market demand is increasing continuously.

[0003] However, the national wild resources of Cyclocarya paliurus are very scarce, the natural regeneration of Cyclocarya paliurus population is poor, and the artificial cultivation technology is relatively backward, resulting in low leaf yield and unable to meet the market demand. At present, the seedling breeding technology of Cyclocarya paliurus has not achieved a fundamental breakthrough, which seriously restricts the development and utilization of Cyclocarya paliurus. In recent years, with the rise of the big health industry, the demand for the construction of Cyclocarya paliurus artificial economic forests has increased sharply. However, problems such as seedling leggy growth and loose plant type are common in traditional cultivation, resulting in low photosynthetic efficiency and increased harvesting cost, which seriously restricts the popularization of large-scale planting. How to realize the plant type regulation of Cyclocarya paliurus seedlings and cultivate dwarf and robust high-quality seedlings has become a technical bottleneck for industrial development. Summary of the Invention

[0004] The purpose of the present invention is to provide a composition for cultivating dwarf Cyclocarya paliurus seedlings, and its method and application.

[0005] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0006] The present invention provides a composition for cultivating dwarf Cyclocarya paliurus seedlings, which is composed of a triiodobenzoic acid solution, phosphate fertilizer and potassium fertilizer. The concentration of the triiodobenzoic acid solution is 90-110 mg / L, and the volume-mass ratio of the triiodobenzoic acid solution, phosphate fertilizer and potassium fertilizer is 2-5 ml: 7-10 g: 17-19 g.

[0007] In the composition of the present invention, triiodobenzoic acid can reduce the seedling height of Cyclocarya paliurus seedlings, promote the growth of seedling ground diameter, and is beneficial to dwarfing Cyclocarya paliurus seedlings, while phosphate fertilizer and potassium fertilizer can regulate the leaf length, leaf width and leaf thickness of Cyclocarya paliurus seedlings. Through scientific proportioning and combining with the composition of the present invention, the dwarfing of Cyclocarya paliurus seedlings can be better achieved.

[0008] The present invention also provides a method for dwarfing Cyclocarya paliurus seedlings by using the above-mentioned composition, which includes the following steps: one month after planting the bare-root seedlings of Cyclocarya paliurus, apply phosphate fertilizer and potassium fertilizer to the roots and spray a triiodobenzoic acid solution with a concentration of 90-110 mg / L.

[0009] Furthermore, the number of root applications is 2-4 times, and the root application frequency is once every 55-60 days.

[0010] Furthermore, the number of spraying times is 2-4 times, and the spraying frequency is once every 9-11 days.

[0011] Furthermore, the application amount of phosphate fertilizer is 7-10 g / plant, and the application amount of potassium fertilizer is 17-19 g / plant.

[0012] The present invention also provides the application of the above-mentioned composition in cultivating dwarf Cyclocarya paliurus seedlings.

[0013] Furthermore, the composition is used to reduce the seedling height of Cyclocarya paliurus.

[0014] Furthermore, the composition is used to increase the leaf length of Cyclocarya paliurus seedlings.

[0015] Furthermore, the composition is used to increase the leaf width of Cyclocarya paliurus seedlings.

[0016] Furthermore, the composition is used to increase the leaf thickness of Cyclocarya paliurus seedlings.

[0017] The present invention has the following beneficial effects:

[0018] The composition provided by the present invention for cultivating dwarf Cyclocarya paliurus seedlings can significantly dwarf the height of Cyclocarya paliurus seedlings, increase the ground diameter growth of Cyclocarya paliurus seedlings, as well as the leaf length, leaf width and leaf thickness of the seedlings. This provides certain theoretical and technical support for promoting the large-scale popularization of Cyclocarya paliurus artificial economic forests. Description of the Drawings

[0019] Figure 1 It is a graph of the growth increment of the seedling height of Cyclocarya paliurus. Different lowercase letters indicate significant differences, p<0.05.

[0020] Figure 2 It is a graph of the growth increment of the ground diameter of Cyclocarya paliurus seedlings.

[0021] Figure 3 It is a graph of the change amount of the height-diameter ratio of Cyclocarya paliurus seedlings.

[0022] Figure 4 It is a graph of the growth increment of the cross-sectional area of the ground diameter of Cyclocarya paliurus seedlings.

[0023] Figure 5 It is a graph of the increased number of branches of Cyclocarya paliurus seedlings.

[0024] Figure 6It is the leaf biomass diagram of Cyclocarya paliurus seedlings.

[0025] Figure 7 It is the stem biomass diagram of Cyclocarya paliurus seedlings.

[0026] Figure 8 It is the leaf length diagram of Cyclocarya paliurus seedlings.

[0027] Figure 9 It is the leaf width diagram of Cyclocarya paliurus seedlings.

[0028] Figure 10 It is the leaf thickness diagram of Cyclocarya paliurus seedlings. Detailed implementation manners

[0029] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments, but it should not be construed as a limitation of the present invention. Unless otherwise specified, the technical means used in the following embodiments are conventional means well-known to those skilled in the art. The materials, reagents, etc. used in the following embodiments can be obtained from commercial sources unless otherwise specified.

[0030] Example 1: Research and optimization of the composition for cultivating dwarf Cyclocarya paliurus seedlings

[0031] 1. Experimental design.

[0032] The test research materials selected 1-year-old bare-root seedlings of Cyclocarya paliurus with basically the same growth vigor of provenances, which were from the Cyclocarya paliurus planting base in Jiujiang City, Jiangxi Province. The fertilizers used in the fertilization test were urea with an effective nitrogen content of 46%, superphosphate with an effective phosphorus content of 14%, and potassium sulfate with an effective potassium content of 52%. The dwarfing agents selected were brassinolide solution, triiodobenzoic acid solution, and chlormequat solution.

[0033] The test was carried out in the Niumulin Nature Reserve in Yongchun County in mid-March 2024, with 1-year-old Cyclocarya paliurus seedlings as the research object. In the sample plot, the planting points were arranged in a square configuration, the hole spacing was 2.0 m × 2.0 m, and the length, width, and depth of the hole were 40 cm × 40 cm × 40 cm. After selecting 1-year-old Cyclocarya paliurus seedlings with the same growth vigor and transplanting them into the sample plot, the topsoil was backfilled. The test took nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, and dwarfing agent as 4 factors, and each factor was set at 4 levels. Fertilization and dwarfing treatments were carried out according to the orthogonal test design, and the scheme is shown in Table 1. The dwarfing fertilization treatment started 1 month after planting and was carried out once every two months for a total of 3 times. The above plant growth regulators chlormequat, triiodobenzoic acid, brassinolide, and water were used to spray the leaves of the plants until the leaves were covered with water droplets but not dripping, and it was sprayed once every 10 days for a total of 3 times. There were 16 treatments in total, with three replicates for each treatment and 10 seedlings for each replicate. During the test period, unified and normal cultivation management was carried out. Strengthen the management of water and fertilizer to ensure that the soil water content was between 50% and 60% as much as possible. The test ended in mid-November 2024, and the whole test lasted for 8 months.

[0034] Table 1: Orthogonal experiment design

[0035]

[0036]

[0037] Note: In the table, levels 1, 2, 3, and 4 of the plant growth regulator represent clear water, chlormequat chloride solution with a concentration of 500 mg / L, tiibenzuron solution with a concentration of 100 mg / L, and brassinolide solution with a concentration of 0.2 mg / L respectively; the fertilization amounts of levels 1, 2, 3, and 4 of nitrogen fertilizer, phosphate fertilizer, and potassium fertilizer are 0 g / plant, 9 g / plant, 18 g / plant, and 27 g / plant respectively.

[0038] II. Measurement of seedling indicators.

[0039] Measurement of growth amount: At the end of the slow seedling stage, measure the initial seedling height (H0) and ground diameter (D0) of each seedling. After 6 months of cultivation management, measure the seedling height (H1) and ground diameter (D1) again. Use a tape measure with a precision of 0.1 mm to measure the seedling height, and use a vernier caliper with a precision of 0.01 mm to measure the ground diameter. Seedling height growth amount = H1 - H0, ground diameter growth amount = D1 - D0, height-diameter ratio = seedling height growth amount / ground diameter growth amount, and height-diameter ratio change amount = final height-diameter ratio - initial height-diameter ratio.

[0040] Determination of branch number and leaf morphological indicators: Determine the primary lateral branches of the seedlings as the branch number. Select the leaves of each seedling that are free from obvious pests and diseases and fully expanded, and measure the leaf length, leaf width, and leaf thickness. Leaf length: Use a vernier caliper with a precision of 0.01 mm to measure the longest part of the leaf; leaf width: Use a vernier caliper with a precision of 0.01 mm to measure the widest part of the leaf; leaf thickness: Use a vernier caliper to measure the thickness of the fresh leaf, avoiding the main vein of the leaf, and take the average of 3 measurements as the leaf thickness of this leaf.

[0041] Determination of biomass: Select seedlings with basically the same growth and no pests and diseases for harvesting. Wash the leaves and stems of each plant with deionized water, then use filter paper to dry the surface moisture, and use an electronic balance to measure the fresh weight of each sample. Place the above-mentioned seedling leaves and stems in an oven at 65 °C and dry them to a constant weight, and use an electronic balance to weigh the dried leaf biomass and stem biomass.

[0042] III. Influence of dwarfing fertilization on the tree shape growth of seedlings.

[0043] 1. Seedling height increment: From Figure 1It can be seen that the growth increment of seedling height in all water treatment groups is higher than that in the growth regulator treatment groups, among which the treatments of A2 and A4 are significantly higher than other treatments. The growth increments of seedling height in the three growth regulator treatments of chlormequat chloride, triiodobenzoic acid and brassinolide reach the lowest in the treatments of C4, T4 and B4 respectively. Among all the treatments, the overall dwarfing effect of triiodobenzoic acid treatment is the best.

[0044] Table 2: Range analysis and variance analysis of seedling height growth increment under different treatments

[0045]

[0046] Note: Different lowercase letters indicate significant differences among different levels of the same factor (p<0.05).

[0047] To explore the influence degree and optimal ratio of four factors, namely plant growth regulator, nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer, on the growth of Cyclocarya paliurus seedlings, range analysis was carried out on the seedling height. As Figure 2 shown, the importance ranking of the four factors is: growth regulator > phosphorus fertilizer > potassium fertilizer > nitrogen fertilizer. Among them, the range of the growth regulator is significantly greater than that of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer, indicating that the growth regulator is the dominant factor affecting the seedling height. From the means of each level, the optimal ratio for seedling dwarfing is 100 mg / L triiodobenzoic acid solution, 0 g / plant of nitrogen fertilizer, 18 g / plant of phosphorus fertilizer, and 18 g / plant of potassium fertilizer.

[0048] Through multiple comparisons of the differences in seedling height growth increment of seedlings under different levels of the same factor, it can be seen that the growth increment of seedling height at level 1 of the growth regulator factor is significantly higher than other levels, while the influence of different levels of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer factors on the seedling height growth increment is not significant. Therefore, triiodobenzoic acid has the best dwarfing effect on Cyclocarya paliurus seedlings, and the optimal levels of nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer can be determined by other indicators.

[0049] 2. Growth increment of seedling ground diameter: As Figure 2 can be seen, the growth increment of the ground diameter fluctuates greatly under different treatments. The growth increment of the ground diameter is the smallest under the treatments of A2, C3, T3 and B1, that is, when the potassium fertilizer is at level 2, indicating that a low application rate of potassium fertilizer is not conducive to the growth of the ground diameter. The growth effect of the ground diameter is the best under the triiodobenzoic acid treatment, but the difference is not obvious.

[0050] Table 3: Range analysis and variance analysis of seedling ground diameter growth increment under different treatments

[0051]

[0052] As shown in Table 3, range analysis was carried out on the ground diameter growth increment. From the influence degree, the importance ranking of the four factors is: potassium fertilizer > nitrogen fertilizer > phosphorus fertilizer > growth regulator. The range of potassium fertilizer is much larger than that of other factors, indicating that potassium fertilizer is the dominant factor affecting the ground diameter growth increment. From the means of each level, the best ratio for the ground diameter growth of seedlings is 100 mg / L triiodobenzoic acid solution, 0 g / plant of nitrogen fertilizer, 27 g / plant of phosphorus fertilizer, and 18 g / plant of potassium fertilizer.

[0053] By conducting multiple comparisons on the differences in the ground diameter growth increments of seedlings under different levels of the same factor, it can be seen that the ground diameter growth increments at level 3 of the potassium fertilizer factor are higher than those of other levels, and are significantly higher than level 2. There is no significant influence on the ground diameter growth increment among different levels of the growth regulator, nitrogen fertilizer, and phosphorus fertilizer factors. The effect of potassium fertilizer level 3 is the best, and the best levels of other factors can be determined jointly considering other indicators.

[0054] 3. Change amount of seedling height-diameter ratio: Figure 3 Among them, except for the A2 treatment, the seedling height-diameter ratios of the remaining treatments all show a downward trend. The height-diameter ratio decreases the most under the treatment of triiodobenzoic acid, and the seedling morphology is the best.

[0055] Table 4: Range analysis and variance analysis of the change amount of seedling height-diameter ratio under different treatments

[0056]

[0057] As shown in Table 4, range analysis was carried out on the change amount of seedling height-diameter ratio. From the influence degree, the importance ranking of the four factors is: growth regulator > potassium fertilizer > phosphorus fertilizer > nitrogen fertilizer. The ranges of the growth regulator and potassium fertilizer are significantly larger than those of nitrogen fertilizer and phosphorus fertilizer, indicating that the change of seedling height-diameter ratio is mainly affected by the growth regulator and potassium fertilizer. From the means of each level, the best ratio for regulating the seedling height-diameter ratio is 100 mg / L triiodobenzoic acid solution, 0 g / plant of nitrogen fertilizer, 27 g / plant of phosphorus fertilizer, and 27 g / plant of potassium fertilizer.

[0058] By conducting multiple comparisons on the differences in the change amounts of seedling height-diameter ratios under different levels of the same factor, there are significant differences among different levels of the growth regulator and potassium fertilizer. There is no significant influence on the change amount of the height-diameter ratio among different levels of the nitrogen fertilizer and phosphorus fertilizer factors. Therefore, the reasonable ratios of nitrogen fertilizer and phosphorus fertilizer should be determined jointly considering other indicators.

[0059] 4. Growth increment of seedling ground diameter cross-sectional area: From Figure 4 it can be seen that the growth increments of the ground diameter cross-sectional area under different treatments fluctuate greatly. The ground diameter growth increments are the smallest under the A2, C3, T3, and B1 treatments, that is, when the potassium fertilizer is at level 2, indicating that low application rates of potassium fertilizer are not conducive to the growth of the ground diameter. Among all the treatments, the ground diameter growth effect is the best under the treatment of triiodobenzoic acid, but the difference is not obvious.

[0060] Table 5: Range analysis and variance analysis of the growth increment of the cross-sectional area of the seedling ground diameter under different treatments

[0061]

[0062] As shown in Table 5, a range analysis was carried out on the growth increment of the cross-sectional area of the ground diameter. From the degree of influence, the importance ranking of the four factors is: potassium fertilizer > nitrogen fertilizer > phosphorus fertilizer > growth regulator. The ranges of potassium fertilizer and nitrogen fertilizer are much larger than those of growth regulator and phosphorus fertilizer, indicating that potassium fertilizer and nitrogen fertilizer are the dominant factors affecting the growth increment of the ground diameter. From the means of each level, the best ratio for the growth of the seedling ground diameter is 100 mg / L triiodobenzoic acid solution, 0 g / plant of nitrogen fertilizer, 0 g / plant of phosphorus fertilizer, and 18 g / plant of potassium fertilizer.

[0063] By conducting a multiple comparison of the differences in the growth increment of the cross-sectional area of the seedling ground diameter under different levels of the same factor, it can be seen that there are significant differences among different levels of the potassium fertilizer factor, while there are no significant effects on the growth increment of the ground diameter among different levels of the growth regulator, nitrogen fertilizer, and phosphorus fertilizer factors. The effect of potassium fertilizer level 3 is the best, and the best levels of other factors can be determined in combination with other indicators.

[0064] 5. Number of increased branches of seedlings: Figure 5 Among them, the number of increased branches in the A1 - A4 treatments shows a downward trend and reaches the highest in the A1 treatment. The number of increased branches in the C1 - C4 treatments shows a trend of first decreasing and then increasing, and the number of increased branches is the largest in the C4 treatment. The number of increased branches in the T1 - T4 treatments and B1 - B4 treatments shows a trend of first increasing and then decreasing with the increase in concentration, and reaches the highest in the T3 and B2 treatments respectively. Among all the treatments, the seedlings have the most branches under the treatment of chlormequat chloride.

[0065] Table 6: Range analysis and variance analysis of the number of increased branches of seedlings under different treatments

[0066]

[0067] The results are shown in Table 6. A range analysis was carried out on the number of increased branches. From the degree of influence, the importance ranking of the four factors is: potassium fertilizer > phosphorus fertilizer > growth regulator > nitrogen fertilizer. The range of potassium fertilizer is greater than that of other factors, indicating that potassium fertilizer is the dominant factor affecting the increase in branches. From the means of each level, the best ratio for the increase in branches of seedlings is 500 mg / L chlormequat chloride solution, 9 g / plant of nitrogen fertilizer, 0 g / plant of phosphorus fertilizer, and 9 g / plant of potassium fertilizer.

[0068] By conducting a multiple comparison of the differences in the number of increased branches of seedlings under different levels of the same factor, it can be seen that the number of increased branches at level 2 of the K fertilizer factor is higher than other levels, and is significantly higher than level 4. There are no significant effects on the number of increased branches among different levels of the growth regulator, nitrogen fertilizer, and phosphorus fertilizer factors. The effect of potassium fertilizer level 2 is the best, and the effects of triiodobenzoic acid and phosphorus fertilizer level 4 are relatively poor.

[0069] IV. Effects of dwarfing fertilization on seedling biomass accumulation.

[0070] 1. Seedling leaf biomass: As Figure 6 shown, the leaf biomass is significantly affected by growth regulators. The seedling leaf biomass of treatments C1 - C4, T1 - T4, and B1 - B4 is higher than that of treatments A1 - A4. Among them, the overall leaf biomass of treatments C1 - C4 and T1 - T4 is higher, and is significantly higher than that of treatments B1 - B4 and A1 - A4. Among all treatments, the leaf biomass is the largest under the chlormequat treatment.

[0071] Table 7: Range analysis and variance analysis of seedling leaf biomass under different treatments

[0072]

[0073] As shown in Table 7, a range analysis was conducted on the leaf biomass. From the degree of influence, the importance ranking of the four factors is: growth regulator > nitrogen fertilizer > phosphorus fertilizer > potassium fertilizer. The range of the growth regulator is significantly greater than other factors, indicating that the growth regulator is the dominant factor affecting the seedling leaf biomass. From the means of each level, the best ratio for increasing the seedling leaf biomass is 500 mg / L chlormequat solution, 9 g / plant of nitrogen fertilizer, 9 g / plant of phosphorus fertilizer, and 9 g / plant of potassium fertilizer.

[0074] A multiple comparison was made on the differences in seedling leaf biomass under different levels of the same factor. The leaf biomass at levels 2 and 3 of the growth regulator factor is significantly higher than that at levels 1 and 4. Among the nitrogen fertilizer, phosphorus fertilizer, and potassium fertilizer factors, the effects of different levels on the leaf biomass are not significant.

[0075] 2. Seedling stem biomass: As Figure 7 shown, there are significant differences in the stem biomass under different growth regulator treatments. The seedling stem biomass of treatments C1 - C4 and T1 - T4 is significantly higher than that of treatments B1 - B4 and A1 - A4. The stem biomass is the largest under the chlormequat treatment.

[0076] Table 8: Range analysis and variance analysis of seedling stem biomass under different treatments

[0077]

[0078] As shown in Table 8, a range analysis was conducted on the stem biomass. From the degree of influence, the importance ranking of the four factors is: growth regulator > nitrogen fertilizer > phosphorus fertilizer > potassium fertilizer. The range of the growth regulator is significantly greater than other factors, indicating that the growth regulator is the dominant factor affecting the seedling stem biomass. From the means of each level, the best ratio for seedling stem biomass accumulation is 500 mg / L chlormequat solution, 27 g / plant of nitrogen fertilizer, 0 g / plant of phosphorus fertilizer, and 18 g / plant of potassium fertilizer.

[0079] Multiple comparisons were made on the differences in seedling stem biomass under different levels of the same factors. Among the growth regulator factors, the stem biomass at level 2 was significantly higher than other levels. Among the nitrogen fertilizer, phosphorus fertilizer, and potassium fertilizer factors, the effects of different levels on stem biomass were not significant.

[0080] V. Effects of dwarfing fertilization on the leaf morphology of seedlings.

[0081] 1. Seedling leaf length: As can be seen from Figure 8 it, the leaf lengths of the seedlings treated with A2, C2, T2, and B2 were the largest among all treatments. The average leaf length was the largest under the treatment with tiabendazole.

[0082] Table 9: Range analysis and variance analysis of seedling leaf length under different treatments

[0083]

[0084] As shown in Table 9, range analysis was carried out on the seedling leaf length. From the degree of influence, the importance ranking of the four factors was: growth regulator > nitrogen fertilizer > phosphorus fertilizer > potassium fertilizer, among which the degrees of influence of the growth regulator, nitrogen fertilizer, and phosphorus fertilizer were quite similar. From the means of each level, the optimal ratio for seedling leaf length was 100 mg / L tiabendazole solution, 9 g / plant of nitrogen fertilizer, 9 g / plant of phosphorus fertilizer, and 18 g / plant of potassium fertilizer.

[0085] Multiple comparisons were made on the differences in seedling leaf length under different levels of the same factors. Among the growth regulator factors, the leaf length at level 3 was significantly higher than other levels.

[0086] 2. Seedling leaf width: As can be seen from Figure 9 it, the leaf widths of the seedlings treated with T1–T4 were generally higher than those of other treatments, indicating that tiabendazole can generally promote the growth of leaf width more effectively.

[0087] Table 10: Range analysis and variance analysis of seedling leaf width under different treatments

[0088]

[0089]

[0090] The results are shown in Table 10. Range analysis was carried out on the seedling leaf width. From the degree of influence, the importance ranking of the four factors was: phosphorus fertilizer > growth regulator > nitrogen fertilizer > potassium fertilizer, among which the degrees of influence of phosphorus fertilizer, growth regulator, and nitrogen fertilizer were quite similar. From the means of each level, the optimal ratio for seedling leaf width was 100 mg / L tiabendazole solution, 9 g / plant of nitrogen fertilizer, 9 g / plant of phosphorus fertilizer, and 18 g / plant of potassium fertilizer.

[0091] Multiple comparisons were made on the differences in seedling leaf length under different levels of the same factors. It was found that the effects of different levels of the four factors on leaf width were not significant.

[0092] 3. Seedling leaf thickness: Figure 10 Among them, the leaf thickness reached the highest under the T2 treatment, increasing by 108.74% compared to the B1 treatment with the lowest leaf thickness. Among all the treatments, the leaf thickness was the highest under the treatment with TIBA (triiodobenzoic acid).

[0093] Table 11: Range analysis and variance analysis of seedling leaf thickness under different treatments

[0094]

[0095] The results are shown in Table 11. Range analysis was carried out on the leaf thickness. From the influence degree, the importance ranking of the four factors is: nitrogen fertilizer > growth regulator > phosphate fertilizer > potassium fertilizer. From the means of each level, the optimal ratio for seedling leaf thickness is 100 mg / L triiodobenzoic acid solution, 9 g / plant of nitrogen fertilizer, 9 g / plant of phosphate fertilizer, and 27 g / plant of potassium fertilizer. Multiple comparisons were made on the differences in seedling leaf thickness at different levels of the same factor. The leaf thickness at level 2 of the nitrogen fertilizer factor was significantly higher than other levels, and there was no significant difference in the influence on leaf thickness among different levels of the growth regulator, phosphate fertilizer, and potassium fertilizer factors.

[0096] VI. Optimal dwarfing fertilization scheme.

[0097] The optimal dwarfing fertilization scheme is determined based on the principle of mainly affecting the tree shape growth index of the seedlings, supplemented by affecting the biomass index of the seedlings, and taking into account the influence on the leaf morphological index of the seedlings. The optimal growth regulator for the increment of seedling height, increment of ground diameter, height-diameter ratio, and increment of ground diameter cross-sectional area is 100 mg / L triiodobenzoic acid, and the N fertilizer is 0 g / plant; the optimal level of P fertilizer for the leaf biomass, leaf length, leaf width, and leaf thickness of the seedlings is 9 g / plant; the optimal level of K fertilizer for the increment of seedling height, increment of ground diameter, increment of ground diameter cross-sectional area, stem biomass, leaf length, and leaf width is 18 g / plant. Therefore, the optimal dwarfing fertilization scheme is 100 mg / L triiodobenzoic acid solution, 0 g / plant of nitrogen fertilizer, 9 g / plant of phosphate fertilizer, and 18 g / plant of potassium fertilizer.

[0098] It should be noted that when the claims of the present invention involve numerical ranges, it should be understood that any value between the two endpoints of each numerical range and any value between the two endpoints can be selected. To avoid redundancy, preferred embodiments of the present invention are described.

[0099] Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications once they know the basic creative concept. Therefore, the appended claims are intended to be construed as including the preferred embodiments and all changes and modifications falling within the scope of the present invention.

[0100] Obviously, those skilled in the art can make various modifications and variations to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention also intends to include these modifications and variations.

Claims

1. A composition for cultivating dwarf Cyclocarya paliurus seedlings, characterized in that, The composition consists of a solution of triiodobenzoic acid, phosphate fertilizer, and potassium fertilizer. The concentration of the triiodobenzoic acid solution is 90 - 110 mg / L, and the volume - mass ratio of the triiodobenzoic acid solution, phosphate fertilizer, and potassium fertilizer is 2 - 5 ml : 7 - 10 g : 17 - 19 g.

2. A method for dwarfing Cyclocarya paliurus seedlings using the composition described in claim 1, characterized in that, It includes the following steps: One month after planting Cyclocarya paliurus bare - root seedlings, apply phosphate fertilizer and potassium fertilizer to the roots and spray a triiodobenzoic acid solution with a concentration of 90 - 110 mg / L.

3. The method of using the composition according to claim 2 for dwarfing Cyclocarya paliurus seedlings, characterized in that, The number of root applications is 2 - 4 times, and the root - application frequency is once every 55 - 60 days.

4. The method of using the composition according to claim 2 for dwarfing Cyclocarya paliurus seedlings, characterized in that, The number of spraying times is 2 - 4 times, and the spraying frequency is once every 9 - 11 days.

5. The method of using the composition according to claim 2 for dwarfing Cyclocarya paliurus seedlings, characterized in that, The application rate of phosphate fertilizer is 7 - 10 g per plant, and the application rate of potassium fertilizer is 17 - 19 g per plant.

6. Use of the composition according to claim 1 in cultivating dwarf Cyclocarya paliurus seedlings.

7. Use of the composition according to claim 6 in cultivating dwarf Cyclocarya paliurus seedlings, characterized in that, The composition is used to reduce the seedling height of Cyclocarya paliurus.

8. Use of the composition according to claim 6 in cultivating dwarf Cyclocarya paliurus seedlings, characterized in that, The composition is used to increase the leaf length of Cyclocarya paliurus seedlings.

9. Use of the composition according to claim 6 in cultivating dwarf Cyclocarya paliurus seedlings, characterized in that, The composition is used to increase the leaf width of Cyclocarya paliurus seedlings.

10. Use of the composition according to claim 6 in cultivating dwarf Cyclocarya paliurus seedlings, characterized in that, The composition is used to increase the leaf thickness of Cyclocarya paliurus seedlings.

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