PCR amplification mechanism for a dPCR integrated microfluidic chip

Abstract

The present invention relates to a PCR amplification mechanism for a dPCR integrated microfluidic chip. The PCR amplification mechanism comprises a preheating flow channel and a circulating flow channel communicating in sequence, wherein the preheating flow channel is used to heat droplets to a first preset temperature; the circulating flow channel includes a plurality of high temperature sectionsand a plurality of low temperature sections arranged at intervals, so that the droplets are subjected to high temperature, low temperature... high temperature and low temperature circulating flow inthe circulating flow channel; the beginning end of the preheating flow channel communicates with the tail end of a droplet output pipeline; and the tail end of the circulating flow channel communicates with a droplet detection mechanism. The tiny droplets are firstly preheated to the first preset and then subjected to high temperature, low temperature...high temperature and low temperature circulating flow in the circulating flow channel, and rapid amplification of the droplets is realized. The amplification time is shortened, and the detection efficiency is greatly improved on the premise ofensuring accuracy.

Description

technical field [0001] The invention relates to the technical field of biological detection, and more specifically, relates to a PCR amplification mechanism for dPCR integrated microfluidic chips. Background technique [0002] For digital droplet PCR technology, it mainly includes three separate technologies, namely droplet generation technology, PCR thermal cycle amplification technology and droplet detection technology. [0003] The PCR amplification is used to cycle the droplet containing the sample solution to heat up and down, so as to meet the temperature and duration requirements required by the PCR amplification. In the prior art, the standard PCR process usually takes several hours to complete, and due to the limitation of PCR amplification, more time is required for detection. [0004] Based on this, the prior art still needs to be improved. . Contents of the invention [0005] In view of the above problems, the present invention aims to provide a PCR amplific...

AI Technical Summary

Problems solved by technology

In the prior art, the standard PCR process usually takes several hours to complete, and due to the limitation of PCR amplification, the detection takes more time

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