PCR fluorescent detector

Abstract

The invention provides a PCR fluorescent detector. In the detector, a PCR reaction tube is arranged in a first through hole, a second through hole and a third through hole, so the bottom of the PCR reaction tube is positioned at the third through hole, the level of PCR reaction liquid in the PCR reaction tube is positioned at the first through hole, the bottom of the PCR reaction tube is heated by a 95 DEG C heating plate, and the level of the PCR reaction liquid in the PCR reaction tube is heated by a 60 DEG C heating plate, thus producing temperature difference between the upper and lower ends of the PCR reaction tube. The temperature difference causes the PCR reaction liquid in the PCR reaction tube to flow from the bottom high-temperature region to the top low-temperature region to form countercurrent. Due to the temperature difference and countercurrent, DNA can be continuously amplified in the PCR fluorescent detector, continuous heating and cooling for reaching the temperature required by different stages of DNA amplification are not needed, therefore, the PCR fluorescent detector provided by the invention can save the amplification time and further shorten the detection time of the PCR detector.

Description

technical field [0001] The invention relates to the technical field of detection devices, in particular to a PCR fluorescence detector. Background technique [0002] PCR (Polymerase Chain Reaction, Polymerase Chain Reaction) technology is a molecular biology technique for amplifying and amplifying specific DNA (deoxyribonucleic acid, deoxyribonucleic acid) sequences in vitro. PCR technology has the characteristics of strong specificity, high sensitivity, low purity requirements, simplicity and rapidity, so it is widely used in molecular biology detection and analysis. [0003] Generally, the process of DNA amplification by PCR technology is: in a mixed system of suitable buffer solution and heat-resistant DNA polymerase, DNA undergoes a high-temperature melting reaction at a temperature of about 95°C, that is, hydrogen bonds between DNA double strands Break to form two complementary single-stranded DNA; the single-stranded DNA undergoes annealing (annealing) reaction mediat...

AI Technical Summary

Problems solved by technology

[0005] The invention provides a PCR fluorescence detector to solve the problem that the detection time of the existing PCR detector is longer

Images