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Serum-free medium for human umbilical cord mesenchymal stem cells and preparation method thereof

A serum-free medium and quality stem cell technology, applied in the field of stem cells, can solve the problems of unsatisfactory cell proliferation rate, poor cell adherence, and cost reduction, and achieve the effects of good cell induction and differentiation potential, simple preparation method, and cost reduction.

Inactive Publication Date: 2015-12-02
广东美赛尔细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the problems existing in the prior art, such as poor cell adhesion, too complex components, and unsatisfactory cell proliferation rate, the inventor obtained a serum-free medium for human umbilical cord mesenchymal stem cells through a large number of tests and screenings. The base does not need to be coated with culture flasks, and is easy to operate, has a high cell proliferation rate, and maintains good immature morphology and stem cell characteristics of stem cells, has good cell differentiation potential, and reduces costs

Method used

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  • Serum-free medium for human umbilical cord mesenchymal stem cells and preparation method thereof
  • Serum-free medium for human umbilical cord mesenchymal stem cells and preparation method thereof
  • Serum-free medium for human umbilical cord mesenchymal stem cells and preparation method thereof

Examples

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Embodiment 1

[0028] The serum-free culture medium of embodiment one people's umbilical cord mesenchymal stem cells

[0029] Serum-free medium for human umbilical cord mesenchymal stem cells, including high-glucose DMEM basal medium, and the following components and their concentrations: recombinant human insulin 5 μg / mL, human serum albumin 0.5 mg / mL, transferrin 5 μg / mL, fibronectin 10ng / mL, vitamin C10μg / mL, biotin 3.5μg / mL, stem cell growth factor 5ng / mL and stem cell factor 5ng / mL.

[0030] Preparation method: Add recombinant human insulin, human serum albumin, transferrin, fibronectin, vitamin C, biotin, stem cell growth factor and stem cell factor to the high-glucose DMEM basal medium at the stated concentration, and mix well , get rid of bacteria through membrane.

Embodiment 2

[0031] The serum-free culture medium of embodiment two umbilical cord mesenchymal stem cells

[0032] Serum-free medium for human umbilical cord mesenchymal stem cells, including high-glucose DMEM basal medium, and the following components and their concentrations: recombinant human insulin 8 μg / mL, human serum albumin 0.5 mg / mL, transferrin 2 μg / mL, fibronectin 15ng / mL, vitamin C15μg / mL, biotin 1μg / mL, stem cell growth factor 2ng / mL and stem cell factor 8ng / mL.

[0033] The preparation method is similar to Example 1.

Embodiment 3

[0034] Example 3 Serum-free Medium of Human Umbilical Cord Mesenchymal Stem Cells

[0035] Serum-free medium for human umbilical cord mesenchymal stem cells, including low-sugar DMEM basal medium, and the following components and their concentrations: recombinant human insulin 2 μg / mL, human serum albumin 3 mg / mL, transferrin 8 μg / mL , fibronectin 5ng / mL, vitamin C 5μg / mL, biotin 8μg / mL, stem cell growth factor 8ng / mL and stem cell factor 2ng / mL.

[0036] The preparation method is similar to Example 1.

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Abstract

The invention provides a serum-free medium for human umbilical cord mesenchymal stem cells and belongs to the technical field of stem cells. The serum-free medium comprises a DMEM basic medium and further comprises recombinant human insulin, human serum albumin, transferrin, fibronectin, vitamin C, biotin, stem cell growth factors and stem cell factors. The serum-free medium for human umbilical cord mesenchymal stem cells provided by the invention needs no enveloping of a culture flask, operating is convenient and simple, cell proliferation rate is high, good stem cell pedomorphism and stem cell characteristics are kept, the serum-free medium has a good potential for cell induced differentiation, and the cost is lowered.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and relates to a serum-free culture medium of human umbilical cord mesenchymal stem cells and a preparation method thereof. Background technique [0002] Human umbilical cord mesenchymal stem cells are derived from umbilical cord Walter's jelly. They are a kind of adult stem cells with obvious plasticity and multipotential differentiation potential. Under the regulation of different growth factors, they can differentiate into different tissue cells derived from three germ layers. Types, such as adipocytes, osteoblasts, hepatic-like cells, cardiomyocyte-like cells, glial cells, neuronal cells, and islet-like cells. [0003] Stem cell growth factor (SCGF) is an early hematopoietic stimulating factor that acts in the bone marrow hematopoietic microenvironment, has a variety of biological activities, and plays a significant role in tissue damage repair and wound healing. There are two forms of hu...

Claims

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Application Information

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IPC IPC(8): C12N5/0775
Inventor 王一飞廖晓凤王巧利刘秋英
Owner 广东美赛尔细胞生物科技有限公司
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