Tissue culture and propagation method for clinacanthus nutans

A technology of tissue culture and crocodile beak flower, which is applied in the field of plant propagation, can solve problems that have not been reported, and achieve the effects of easy promotion, low cost, and easy configuration

Inactive Publication Date: 2018-08-28
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But, up to now, the research on the tissue culture of crocodile mouth flower has not been reported in China

Method used

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  • Tissue culture and propagation method for clinacanthus nutans

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Using the stem section of the Alligatoria japonica plant collected from the South China Botanical Garden of the Chinese Academy of Sciences as an explant, after cleaning the stem section explant with tap water, first scrubbing and disinfecting the surface with a volume fraction of 75% alcohol aqueous solution for 10 seconds, and eluting with sterile water for 2 seconds After that, immerse in 0.1% mercuric chloride solution in mass volume percentage for 8 minutes, wash with sterile water for 3 times, and finally cut the stem into 1 cm size, inoculate it on the induction medium and cultivate it. The culture condition is 22-28 ℃, the light intensity is 50 μmol m -2 the s -1 Axillary buds were induced after 10 days of cultivation under the condition of light 14h / d, and then the axillary buds were transferred to the induction medium to continue culturing, and adventitious buds were induced after 30 days; then the adventitious buds were transferred to the propagation medium f...

Embodiment 2

[0032] Using the stem section of the Alligatoria japonica plant collected from the South China Botanical Garden of the Chinese Academy of Sciences as an explant, after cleaning the stem section explant with tap water, first scrubbing and disinfecting the surface with a volume fraction of 75% alcohol aqueous solution for 10 seconds, and eluting with sterile water for 2 seconds After that, immerse in 0.1% mercuric chloride solution in mass volume percentage for 8 minutes, wash with sterile water for 3 times, and finally cut the stem into 1 cm size, inoculate it on the induction medium and cultivate it. The culture condition is 22-28 ℃, the light intensity is 45 μmol m -2 the s -1 Axillary buds were induced after 14 days of cultivation under the condition of light 14h / d, and then the axillary buds were transferred to the induction medium to continue culturing, and adventitious buds were induced after 30 days; then the adventitious buds were transferred to the propagation medium f...

Embodiment 3

[0035] Using the stem section of the Alligatoria japonica plant collected from the South China Botanical Garden of the Chinese Academy of Sciences as an explant, after cleaning the stem section explant with tap water, first scrubbing and disinfecting the surface with a volume fraction of 75% alcohol aqueous solution for 10 seconds, and eluting with sterile water for 2 seconds After that, immerse in 0.1% mercuric chloride solution in mass volume percentage for 8 minutes, wash with sterile water for 3 times, and finally cut the stem into 1 cm size, inoculate it on the induction medium and cultivate it. The culture condition is 22-28 ℃, the light intensity is 45 μmol m -2 the s -1 Axillary buds were induced after 14 days of cultivation under the condition of light 14h / d, and then the axillary buds were transferred to the induction medium to continue culturing, and adventitious buds were induced after 30 days; then the adventitious buds were transferred to the propagation medium f...

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Abstract

The invention discloses a tissue culture and propagation method for clinacanthus nutans. The method can acquire a large number of clinacanthus nutans cultivating seedlings in a short term and achieveeffective protection and propagation of the clinacanthus nutans. The method is simple in operation and easy to carry out, the used culture medium and reagent are easy to prepare, the cost is low, andthe method can be popularized in a wide range.

Description

technical field [0001] The invention belongs to the field of plant propagation, and in particular relates to a method for rapid propagation of crocodile flower tissue culture. Background technique [0002] Clinacanthus nutans is a tall herbaceous plant belonging to the genus Clinacanthus nutans of the family Acanthaceae. It is currently distributed in Guangdong, Guangxi, Yunnan and other places. Dai medicine is used to treat bruises. In Guangdong and Guangxi, it is mostly used as a wild vegetable. The medicine and food are of the same origin, and it can treat liver diseases and rheumatism. Modern research shows that crocodile mouth flower has good anti-cancer, anti-inflammatory, immune regulation, blood fat-lowering and other effects. [0003] Using plant explants to cultivate on solid medium to obtain callus or complete plants is plant tissue culture, also known as in vitro culture or explant culture. Since different types of plants are very different in terms of culture ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王强陈国华陈冬怡杨国陈红锋
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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