Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation for clinacanthus nutans extract and application thereof in anti-tumor aspect

A technique of extracting the herb and its extracts, which are applied in the direction of antineoplastic drugs, medical preparations containing active ingredients, and drug combinations, etc.

Inactive Publication Date: 2018-02-02
HAINAN NORMAL UNIVERSITY +1
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few research reports on the mechanism of anti-tumor effect of S.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation for clinacanthus nutans extract and application thereof in anti-tumor aspect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Dried the stems of Herba Youtun, crushed and sieved, the powder was extracted with 95% ethanol at room temperature for 3 times, each time for 6 days, the extracts were combined, filtered, concentrated under reduced pressure to recover ethanol to obtain ethanol crude extract, the ethanol crude extract Add 2L of distilled water to dilute, and then extract 5 times with an equal volume of petroleum ether to obtain a petroleum ether extract. Extract the remaining aqueous solution with an equal volume of ethyl acetate until the organic phase is nearly colorless to obtain an ethyl acetate extract. Extracted 6 times with water-saturated n-butanol to obtain n-butanol extract.

[0018] Separately concentrate the petroleum ether extract, ethyl acetate extract and n-butanol extract under reduced pressure to obtain the extract, and after vacuum drying, the powder obtained is the petroleum ether extract, ethyl acetate extract and n-butanol Extract.

Embodiment 2

[0020] Dry, crush and sieve the leaves of Udon grass, extract the powder with 85% ethanol at room temperature for 4 times, each time for 7 days, combine the extracts, filter, concentrate under reduced pressure and recover ethanol to obtain ethanol crude extract, and ethanol crude extract Add 3L of distilled water to dilute, then extract 4 times with an equal volume of petroleum ether to obtain a petroleum ether extract, extract the remaining aqueous solution with an equal volume of ethyl acetate until the organic phase is nearly colorless, and obtain an ethyl acetate extract, and then refill the remaining aqueous solution. Extract 5 times with water-saturated n-butanol to obtain n-butanol extract.

[0021] Concentrate the petroleum ether extract, ethyl acetate extract and n-butanol extract under reduced pressure respectively to obtain the extract, and after vacuum drying, the powder obtained is the petroleum ether extract, ethyl acetate extract and n-butanol Extract.

Embodiment 3

[0023] Dried the root of Udun grass, crushed and sieved, the powder was extracted with 75% ethanol at room temperature for 4 times, each time for 7 days, the extracts were combined, filtered, concentrated under reduced pressure to recover ethanol to obtain ethanol crude extract, the ethanol crude extract was added to Dilute with 3L of distilled water, and then extract 4 times with an equal volume of petroleum ether to obtain a petroleum ether extract. Extract the remaining aqueous solution with an equal volume of ethyl acetate until the organic phase is nearly colorless to obtain an ethyl acetate extract. The remaining aqueous solution is then water Saturated n-butanol was extracted 5 times to obtain n-butanol extract.

[0024] Concentrate the petroleum ether extract, ethyl acetate extract and n-butanol extract respectively under reduced pressure to obtain the extract, and after vacuum drying, the powder is obtained as Youdun grass root petroleum ether extract, ethyl acetate ex...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparation method for clinacanthus nutans extract. The preparation method specifically comprises the following steps: drying, smashing and sieving the plant part of the clinacanthus nutans, extracting by using ethanol, decompressing to obtain ethanol coarse extract by using an ethanol recycling agent, adding water to the ethanol extract and diluting, extracting by usingan organic solvent, decompressing and concentrating extract liquid to obtain the extract, vacuum-drying the extract to obtain powder which is the clinacanthus nutans extract namely. The extract has the remarkable inhibiting effect to human leukemia, liver cancer, lung cancer, breast cancer and colon cancer cells through an in-vitro cytotoxic activity test, and has the better anti-tumor activity, and can be used for preparing an anti-tumor drug.

Description

technical field [0001] The invention belongs to the field of plant application, and relates to a plant active extract and its application, in particular to the preparation of an extract of Herba japonica and its application in antitumor. Background technique [0002] Malignant tumors have become an important cause of endangering human life and health, and have become the second killer of humans after cardiovascular and cerebrovascular diseases. At present, there is no specific drug for the treatment of tumor diseases, and traditional Western medicine is not effective in treating tumors. Therefore, Searching for drugs with antitumor activity from natural medicinal plants has become the mainstream trend of contemporary drug research. Natural products have always been an important source of drugs and drug lead compounds due to their structural complexity, diversity, novelty and unique functions. According to the statistics of Dr. Newman DJ of the National Cancer Institute of t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/19A61P35/00A61P35/02A61K125/00A61K135/00A61K127/00
CPCA61K36/19A61K2236/33A61K2236/51
Inventor 宋小平韩长日邵泰明
Owner HAINAN NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com