Method for extracting clinacanthus nutans active substances through ultrasonic assistance

An ultrasound-assisted extraction and active substance technology, which is applied in the field of plant resource development, can solve the problems that the research on the extraction technology of the active substance of the grass is rare, and there is no extraction of various active substances from the grass, so as to shorten the extraction time and improve the permeability Sexuality and the effect of reducing production costs

Inactive Publication Date: 2016-02-17
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing processing technology of Uduncai concentrates on the direct processing of Uduncao leaves into products. There are few studies on the extr

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The raw materials are cleaned, drained, dried, pulverized with an ultra-fine mill, and passed through a 200-mesh sieve to obtain Youduncao powder; the obtained Youduncao powder is kept fresh at 5°C in a refrigerator for future use. Adjust the ultrasonic power of the ultrasonic cleaning machine to 156W, the ultrasonic temperature to 45°C, and the ultrasonic time to 30 minutes; weigh 4.541g of Youduncao powder into a 500mL conical flask, and add 250.0mL of tap water according to the material-to-liquid ratio of 1:55 (m / V) ; Then put the Erlenmeyer flask into an ultrasonic cleaning machine with adjusted ultrasonic conditions in advance for ultrasonic treatment; centrifuge the ultrasonic solution for 15 minutes at 6000r / min, and the supernatant obtained is the active substance extract of Herba Youtuncao. The yield of polyphenols in the extract was determined to be 9.387mg / g, the yield of flavonoids was 28.785mg / g, the yield of triterpenes was 23.665mg / g, and the yield of vita...

Embodiment 2

[0019] The raw materials are cleaned, drained, dried, pulverized with an ultra-fine mill, and passed through a 200-mesh sieve to obtain Youduncao powder; the obtained Youduncao powder is kept fresh at 5°C in a refrigerator for future use. Adjust the ultrasonic power of the ultrasonic cleaning machine to 144W, the ultrasonic temperature to 50°C, and the ultrasonic time to 30 minutes; weigh 5.0920g of Youduncao powder into a 100mL conical flask, and add 50.0mL of tap water according to the ratio of material to liquid 1:10 (m / V) ; Then put the Erlenmeyer flask into an ultrasonic cleaning machine with adjusted ultrasonic conditions in advance for ultrasonic treatment; centrifuge the ultrasonic solution for 15 minutes at 6000r / min, and the supernatant obtained is the active substance extract of Herba Youtuncao. The yield of polyphenols in the extract was determined to be 4.720mg / g, the yield of flavonoids was 23.130mg / g, the yield of triterpenes was 12.432mg / g, and the yield of vita...

Embodiment 3

[0021] The raw materials are cleaned, drained, dried, pulverized with an ultra-fine mill, and passed through a 200-mesh sieve to obtain Youduncao powder; the obtained Youduncao powder is kept fresh at 5°C in a refrigerator for future use. Adjust the ultrasonic power of the ultrasonic cleaning machine to 132W, the ultrasonic temperature to 55°C, and the ultrasonic time to 20 minutes; weigh 1.0236g of Youduncao powder into a 100mL conical flask, and add 50.0mL of tap water according to the material-to-liquid ratio of 1:50 (m / V) ; Then put the Erlenmeyer flask into an ultrasonic cleaning machine with adjusted ultrasonic conditions in advance for ultrasonic treatment; centrifuge the ultrasonic solution for 15 minutes at 6000r / min, and the supernatant obtained is the active substance extract of Herba Youtuncao. The yield of polyphenols in the extract was determined to be 7.365 mg / g, the yield of flavonoids was 23.896 mg / g, the yield of triterpenes was 17.929 mg / g, and the yield of v...

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Abstract

The invention discloses a method for extracting clinacanthus nutans active substances through ultrasonic assistance. The method mainly comprises the steps that 1, raw materials are cleaned up, drained, dried, ground through a superfine grinding mill and screened with a 200-mesh sieve, and then clinacanthus nutans powder is obtained; 2, after being subpackaged, the clinacanthus nutans powder is placed into a refrigerator and preserved at 5 DEG C for standby application; 3, the ultrasonic power, ultrasonic temperature and ultrasonic time of an ultrasonic cleaner are regulated; 4, a certain amount of the clinacanthus nutans powder is accurately weighed, and an appropriate amount of water is added according to the material-liquid ratio; 5, the clinacanthus nutans powder is put into ultrasonic waves of which the ultrasonic conditions are regulated in advance for ultrasonic treatment, wherein the conditions are set as followed: the ultrasonic time is set for 10-50 min, the ultrasonic temperature is set at 30 DEG C-70 DEG C, the material-liquid ratio is set to be 1:20-1:60, and the ultrasonic power is set at 72-216 W; 6, the solution processed through the ultrasonic waves are centrifuged at 6000 r/min for 15 min, and a supernatant solution, that is, a clinacanthus nutans active substance extracting solution is obtained.

Description

technical field [0001] The invention belongs to the technical field of exploiting plant resources, and in particular relates to a method for extracting four active substances, flavonoids, polyphenols, triterpenes and vitamin C, from Herba sativa by ultrasonic waves. Background technique [0002] Wovengrass is a tall herbaceous plant that grows in low-altitude sparse forests near the equator or in moist sandy soil in shrubs. It is widely distributed in Southeast Asia, and it is wild in Hainan, Guangdong, Guangxi, and Yunnan in my country. Youduncao is generally used as a whole plant or leaf as medicine. It tastes sweet, slightly bitter, and cool in nature. It enters the liver and kidney channels. Insect or snake bites. It is understood that in Wuzhishan and other areas of Hainan, the grass is eaten as vegetables, as tea, and the leaves of the grass are squeezed to drink. However, because it is mainly concentrated in ethnic minority areas, no written historical materials have...

Claims

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Application Information

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IPC IPC(8): A61K36/19A23L33/00A23L33/105
CPCA23V2002/00A61K36/19A61K2236/15A61K2236/331A61K2236/53A23V2200/30A23V2250/21A23V2300/48
Inventor 段振华于群刘兵
Owner HAINAN UNIVERSITY
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