Double tetrahydrofuran lignin, preparation method and application thereof

A kind of parallel double tetrahydrofuran, lignan technology, applied in the direction of organic chemistry, drug combination, anti-tumor drugs, etc., can solve the problems of a wide variety, difficult separation and structure identification, etc.

Active Publication Date: 2017-12-29
HAINAN NORMAL UNIV +1
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Problems solved by technology

From the point of view of the parallel ditetrahydrofuran lignans that have been isolated so far, only the types and stereo configurations of their hydrocarbon chains and oxygen-containing substituents on the benzene ring are different, resulting in a wide variety of such compounds. and caused difficulties in the separation and structure identification of

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  • Double tetrahydrofuran lignin, preparation method and application thereof
  • Double tetrahydrofuran lignin, preparation method and application thereof
  • Double tetrahydrofuran lignin, preparation method and application thereof

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Embodiment ）

[0024] The structural formula of the ditetrahydrofuran lignans of the present embodiment is shown in formula (I):

[0025]

[0026] (I).

[0027] The preparation method of the double tetrahydrofuran lignans comprises the following steps:

[0028] ①After pulverizing 9.5kg of naturally air-dried Worcesterwort, soak and reflux extraction with 85% ethanol for 3 times, each time for 7 days, combine the collected reflux liquids and distill under reduced pressure to obtain 550.2g of total ethanol Extract, the extract is viscous brown. Then the total ethanol extract was uniformly dispersed in 3.0L distilled water, and extracted with petroleum ether (3×3.5L) and ethyl acetate (4×4.0L) in sequence to obtain petroleum ether part extract (200g), ethyl acetate Ester site extract (10.2g).

[0029] The stems of C. sativa used in this example were collected from Wuzhishan City, Hainan Province, and the specimens were stored in the Key Laboratory of Tropical Medicinal Phytochemistry, Min...

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Abstract

The invention discloses a double tetrahydrofuran lignin, a preparation method and an application thereof. The preparation of the lignin comprises the following steps: (1) crushing clinacanthus nutans and then soaking in ethyl alcohol, refluxing and extracting, thereby acquiring total ethyl alcohol extract, and then uniformly dispersing in distilled water and using petroleum ether and ethyl acetate for extracting in turn; (2) treating the petroleum ether part extract with silica gelcolumn chromatography, adopting a petroleum ether-ethyl acetate solvent system for gradient eluting, collecting, concentrating and mixing the same flow components; (3) performing reverse phase silica gel column chromatography separation, Sephadex LH-20 sephadex column chromatography separation and high performance liquid chromatography separation on the flow component acquired by eluting with petroleum ether and ethyl acetate at ratio of 5:1, thereby acquiring the double tetrahydrofuran lignin. According to the invention, the double tetrahydrofuran lignin extracted from clinacanthus nutans has inhibitory activity to cervical cancer Hela cells, breast cancer MCF-7 cells and lung adenocarcinoma A549 cells.

Description

technical field [0001] The invention relates to a natural plant extract, in particular to a ditetrahydrofuran lignan and its preparation method and application. Background technique [0002] There are two species of Clinacanthus Nees in the family Acanthaceae, mainly distributed in tropical South China to Malaysia, Java, Kalimantan and other places. There is one species distributed in our country, Clinacanthus nutans, also known as crocodile mouth flower, Sabah snake grass, green arrow and so on. The plant is mainly produced in Hainan, Guangdong, Guangxi, Yunnan and other provinces. It is mainly used for clearing away heat and detoxification, promoting dampness, dispelling blood stasis and reducing swelling, promoting blood circulation and regulating menstruation, and relieving pain among the people. At present, it has been planted on a large scale in Wuzhishan City, Hainan Province. After reviewing the relevant literature, it was found that there are few research reports ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D493/04A61P35/00
CPCC07D493/04
Inventor 宋小平李小宝韩长日陈光英刁鸿章
Owner HAINAN NORMAL UNIV
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