Straw degradation actinomycete and application thereof
A technology of liberating threads and straws, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of less research and application of actinomycetes, and achieve the effects of easy industrial implementation, low cultivation cost, and convenient operation
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Embodiment 1
[0024] Isolation, purification and preservation of embodiment 1 Streptomyces griseorubens (Streptomyces griseorubens)
[0025] Take rotten straw and soil from the paddy field of Jiaoda Pujiang Base, Pujiang Town, Minhang District, Shanghai as samples, and adopt the method of shake flask enrichment culture. After the different samples are ground and mixed, take 10g, put them in 100mL enrichment medium, and keep at 30℃ , 150rpm constant temperature culture. Afterwards, every 5 days, 10% of the inoculum was transferred to another enriched medium, and the acclimatization was repeated 4 times. The enrichment medium adopts the medium with CMC-Na as the sole carbon source.
[0026] Take 100 μL of the enrichment solution and spread it on the CMC-Congo red medium plate, such as figure 2 As shown, place it in a constant temperature and humidity incubator at 30°C and culture it upside down, and a transparent hydrolysis circle will appear after 5 days. Pick a small amount of bacterial...
Embodiment 2
[0028] Identification of Example 2 Streptomyces griseorubens (Streptomyces griseorubens)
[0029] On November 28, 2011, the China Industrial Microbiology Collection Center used the internationally recognized identification method for actinomycetes - multiphase identification to identify the JSD-1 bacteria at the genus level. Identification included observation of microbial morphological characteristics, experiments on physiological and biochemical characteristics, and phylogenetic analysis of 16S rDNA sequences. The bacteria were identified as Streptomyces griseorubens.
[0030] The Streptomyces grisea was named JSD-1, and it was preserved in the General Microorganism Center (CGMCC) of China Committee for Microbial Culture Collection (CGMCC) on January 9, 2012. Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing , Chinese Institute of Microbiology, deposit number is CGMCC No.5706.
Embodiment 3
[0031] Example 3 Test of Cellulase, Ligninase, Pectinase and Hemicellulase Production Ability of Streptomyces grisea of the present invention
[0032] First, use the corresponding enzyme detection plate to measure the enzyme production of the bacteria. Congo red plate can be used for the detection of cellulose, hemicellulose and pectin, while the three lignin degrading enzymes need guaiacol, aniline blue, etc. to test.
[0033] After the test is completed, the DNS method is used to measure the activities of the two cellulase CMCase and FPase in the fermentation broth, which are 55.69U / ml and 21.23U / ml respectively; the DNS method is also used to measure the activities of pectinase and xylanase 3.66U / ml and 4.27U / ml. Wherein, one unit of enzyme activity is the amount of enzyme that generates 1 μg of monosaccharide product per milliliter of enzyme solution catalyzing reactants per minute.
[0034] ABTS, veratrol, Mn 2+ As the reaction substrate, the activities of laccase (L...
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