84 results about "Lignin-modifying enzyme" patented technology

The invention discloses a preparation method of a bioorganic fertilizer. The method is characterized by adopting a complex microorganism bacterium agent formed by bacillus subtilis, aspergillus oryzae, trichoderma reesei, phanerochaete chrysosporium and geobacillus stearothermophilus as a decomposition agent to degrade straws to prepare the bioorganic fertilizer. The preparation method has the beneficial effects that the strain composition of the complex microorganism bacterium agent is new flora composition, which takes full advantage of the physiological activities of strains to generate cellulose, hemicellulose and biological enzymes, such as ligninase, to accelerate the decomposition process of straws, thus preparing the bioorganic fertilizer; the organic straw fertilizer prepared by the preparation method is used as a base fertilizer, so that the contents of available nitrogen, rapidly available phosphorus, potassium and organic matters in soil are higher than those of available nitrogen, rapidly available phosphorus, potassium and organic matters in the soil in which straws are directly used and fertilizers are not applied; the urease activity and cellulase activity of the soil are obviously improved compared with those of the soil in which straws are directly used and fertilizers are not applied.

Described herein are methods for increasing the amount of protein secreted by a cell. In one case, a cell is provided which contains a heterologous nucleic acid encoding a protein having unfolded protein response modulating activity and a heterologous nucleic acid encoding a protein of interest to be secreted. In one case, the protein having unfolded protein response modulating activity is selected from the proteins selected from the group consisting of HAC1, PTC2 and IRE1. The protein of interest can be any secreted protein such as a therapeutic or an industrial enzyme. For example the protein can be selected from the group consisting of lipase, cellulase, endo-glucosidase H, protease, carbohydrase, reductase, oxidase, isomerase, transferase, kinase, phosphatase, alpha-amylase, glucoamylase, lignocellulose hemicellulase, pectinase and ligninase.

Disclosed herein are compositions containing enzymes, particularly acting at the interface between two immiscible phases where the rate of enzymatic activity is increased by addition of a blend of surfactant(s) and a mixture derived from yeast fermentation, that contain non-enzymatic exo-proteins released by yeast in response to a non-lethal stress. The enzymes include those that work at the interface between an aqueous solution and a water immiscible phase, liquid or solid, such as oil, fat, cellulose, lignin, etc. including, but not limited to the following or combinations thereof: lipases, polysaccharase, lignase, cellulase and the like, in which the substrate of an enzymatic reaction forms a phase, segregated from the aqueous solution in which the enzymes are typically operating. Disclosed herein are methods for improving a washing solution with the use of these compositions, where the enzyme-protein-surfactant solution can be used in such applications as: laundry, spot remover, pre-laundry, dishes, hard surface cleaning, wastewater treatment, cellulose breakdown as in ethanol production, lignin utilization, environmental remediation, industrial cleaning, and agricultural applications.

The present invention provides transgenic plants which after harvest degrade the lignin and cellulose therein to fermentable sugars which can further be fermented to ethanol or other products. In particular, the transgenic plants comprise ligninase and cellulase genes from microbes operably linked to a DNA encoding a signal peptide which targets the fusion polypeptide produced therefrom to an organelle of the plant, in particular the chloroplasts. When the transgenic plants are harvested, the plants are ground to release the ligninase and cellulase which then degrade the lignin and cellulose of the transgenic plants to produce the fermentable sugars.

The invention relates to an enzyme stabilizer comprising a lignin derivative produced by reaction between lignin and a hydrophilic compound, and to a method of saccharifying lignocellulosic biomass which employs the enzyme stabilizer. According to the invention it is possible to accomplish effective saccharification of cellulosic biomass with saccharifying enzymes, by enhancing saccharifying enzyme activity and preventing nonspecific adsorption of saccharifying enzyme onto substrate.

The invention discloses a production method of a complex enzyme liquid for chemi-mechanical pulping. The production method is characterized by comprising the following steps: weighing various component enzymes, mixing and compounding the various component enzymes in a compounding tank, preventing corrosion, inspecting, packaging and storing in a storeroom at 5 DEG C, wherein the various component enzymes comprise alkaline pectinase, xylanase, mannose and ligninase. The application of the complex enzyme liquid in chemi-mechanical pulping is realized as follows: before steaming paper-making raw materials, adding the complex enzyme liquid to the paper-making raw materials through a metering pump, uniformly mixing the complex enzyme liquid with the paper-making raw materials for enzymatic hydrolysis under enzymatic treatment conditions that the temperature is 40-60 DEG C and enzymatic hydrolysis duration is 30-240 minutes; after the enzymatic hydrolysis, performing following processes as normal. The production method, through coupling synergistic effect of multiple enzymes of the complex enzyme liquid in chemi-mechanical pulping, can be used for improving the whiteness of pulp by 2-5% and reducing the pulp yellowing under a condition of guaranteeing high yield and low cost of pulping; the production method can be used for reducing the dosage of chemical raw materials by 20-30% and reducing the pulp grinding energy consumption by 18-30%; meanwhile, the production method can be used for reducing pollution and improving the pulp quality.