Production method and application of compound enzyme solution for chemical mechanical pulping
A technology of chemical machinery and compound enzyme solution, which is applied in the direction of biochemical equipment and methods, oxidoreductase, and microbial/enzyme cellulose treatment, which can solve the problems of pulp yellowing, high energy consumption, equipment wear, and single enzyme species and other issues, to achieve the effect of reducing energy consumption of refining, reducing pollution and improving quality
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Embodiment 1
[0015] A production method of a compound enzyme solution for chemical mechanical pulping. The enzymes of each component are weighed, mixed and prepared in a compound tank, preserved, inspected, packaged, and then stored in a warehouse at 5°C. The enzymes of each component are alkali The alkaline pectinase, xylanase, mannanase and ligninase; the alkaline pectinase is CCTCCNO: M2010004 bacterial strain culture, shake flask fermentation, seed tank to fermenter, filter, remove Prepared after bacteria and antiseptic treatment; the xylanase is prepared after CGMCCNO: 5466 strains through strain culture, shake flask fermentation, seed tank to fermenter, filtration, sterilization, and antiseptic treatment; the mannanase CGMCCNO: 6226 strains were cultured by strains, fermented in shake flasks, seed tanks were transferred to fermenters, filtered, sterilized, and antiseptically treated; , prepared from seed tank to fermenter, filtered, sterilized, and preservative treated; the activity ...
Embodiment 2
[0060] The activity of alkaline pectinase in the compound enzyme liquid described in this embodiment is 1300u / ml, the activity of xylanase is 1500u / ml, the activity of mannanase is 800u / ml, and the activity of ligninase is 180u / ml.
[0061] The papermaking raw material described in this embodiment is poplar wood chips.
[0062] The chemical mechanical pulping described in this embodiment is APMP alkaline hydrogen peroxide chemical mechanical pulping.
[0063] In this example, the amount of compound enzyme liquid added and the mass percentage of absolute dry papermaking raw materials are 1.5%, the enzyme treatment conditions are: temperature 50°C, pH 8.0, enzymolysis time 230 minutes, after the enzymolysis is completed, the subsequent process is as usual conduct. That is, poplar wood chips are enzymatically hydrolyzed, steamed, and DTPA and Na 2 SO 3 Extrusion prepreg, adding NaOH and H in two stages 2 o 2 Extrusion prepreg, adding NaOH and H in three stages 2 o 2 Extr...
Embodiment 3
[0067] The activity of alkaline pectinase in the compound enzyme liquid described in this embodiment is 1200u / ml, the activity of xylanase is 1300u / ml, the activity of mannanase is 700u / ml, and the activity of ligninase is 130u / ml.
[0068] The papermaking raw material described in this embodiment is reed.
[0069] The chemical mechanical pulping described in this embodiment is BCTMP bleached chemical thermomechanical pulping.
[0070] In this example, the amount of compound enzyme liquid added and the mass percentage of absolute dry papermaking raw materials are 1.5%, the enzyme treatment conditions are: temperature 55°C, pH 8.0, enzymolysis time 180 minutes, after the enzymolysis is completed, the subsequent process is as usual conduct. That is, the reed is pulped after enzymatic hydrolysis, steaming, chemical impregnation, preheating, pressure refining, screening, and bleaching.
[0071] After the raw materials are pretreated with compound enzyme solution, the consumpti...
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