Pithecellobium clypearia extracts and application of extract in preparation of medicines for treating methicillin-resistant staphylococcus aureus

A staphylococcus, extract technology, applied in the direction of antibacterial drugs, plant/algae/fungus/moss components, pharmaceutical formulations, etc., can solve the problem of unreported drug application

Active Publication Date: 2013-11-13
HUACHENG PHARMA FACTORY GAUNGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are literatures disclosing that the simple extracts of monkey earrings in water and ethanol have antiviral effects, but there are no reports on the other uses of the above extracts or the pharmaceutical application of the extracts prepared by other methods

Method used

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  • Pithecellobium clypearia extracts and application of extract in preparation of medicines for treating methicillin-resistant staphylococcus aureus
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  • Pithecellobium clypearia extracts and application of extract in preparation of medicines for treating methicillin-resistant staphylococcus aureus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of monkey earring water extract and its extract

[0030] Monkey earrings (Pithecellobium clypearia Benth.) were provided by Guangzhou Huacheng Pharmaceutical Factory. 300g of monkey earrings were decocted twice with 3000ml of water for 2 hours each time, filtered; the filtrates were combined and concentrated to obtain 81.9g of extract (water extract). Take 54.6g of extract, suspend in 200ml of water, extract 200ml each time with petroleum ether, ethyl acetate, and n-butanol, extract three times with each solvent, combine the extracts respectively, and concentrate to obtain petroleum ether extract 0.9 g, ethyl acetate extract 14.8g, n-butanol extract 11.7g, water layer extract 27.1g.

Embodiment 2

[0032] Screening of monkey earring water extract and its pharmacological effects against methicillin-resistant Staphylococcus aureus (MRSA)

[0033] 1 Experimental method

[0034] 1) Determination of minimum inhibitory concentration (MIC):

[0035] Monkey earring water extract and its extract, erythromycin, ceftriaxone sodium, levofloxacin, and vancomycin were respectively diluted in a series of doubling ratios in MH broth medium, and the inoculum was adjusted to 1.0×10 5 CFU / ml. After incubating at 35°C for 24 hours, the lowest concentration of antibacterial drug without precipitation is its minimum inhibitory concentration (MIC).

[0036] 2) Determination of minimum bactericidal concentration (MBC):

[0037] Pipette 50 μl from the aseptic growth well of item 1) onto a blood plate, spread evenly, incubate at 35°C for 24 hours, count the colonies, and reduce the number of viable bacteria in the initial experiment by 99.9% or above. The concentration is its minimum bacteric...

Embodiment 3

[0054] Preparation of monkey earring 10% ethanol extract and its extract

[0055] Monkey earrings (Pithecellobium clypearia Benth.) were provided by Guangzhou Huacheng Pharmaceutical Factory. 300 g of monkey earrings were extracted twice with 3000 ml of 10% ethanol under reflux, each time for 2 hours, and filtered; the filtrates were combined and concentrated to obtain 95.1 g of extract (10% ethanol extract). Get 63.4g of medicinal extract, extract with petroleum ether, ethyl acetate, n-butanol respectively, each solvent extracts three times, each time 200ml, combine each extract respectively, concentrate to obtain petroleum ether extract 1.2g, ethyl acetate extract 17.3g, n-butanol extract 16.3g, aqueous layer extract 28.6g.

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Abstract

The invention discloses water, ethanol and ethanol aqueous extracts of traditional Chinese medicine pithecellobium clypearia and application of corresponding petroleum ether, ethyl acetate, normal butanol and a water extractants in preparation of medicines for treating methicillin-resistant staphylococcus aureus (MRSA) and antibiotics anti-MRSA sensitization medicines. Meanwhile, the invention further discloses a preparation method of the extracts or extractants. Experimental results show that water, 10% ethanol, 30% ethanol, 60% ethanol, 95% ethanol extracts of pithecellobium clypearia and corresponding ethyl acetate, normal butanol and water extractants have stronger anti-MRSA effect, wherein the activity of the ethyl acetate extracting part of the 60% ethanol extract of pithecellobium clypearia is the strongest, and the ethyl acetate extracting part of the 60% ethanol extract of pithecellobium clypearia has sensitization effect on erythrocin, ceftriaxone sodium, levofloxacin for treating MRSA.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and relates to a new application of a Chinese herbal medicine monkey earring (Pithecellobium clypearia Benth.) extract. Background technique [0002] As we all know, current bacterial drug resistance has become a major public health problem worldwide, and the abuse of antibiotics is an important reason for bacterial drug resistance. Methicillin-resistant Staphylococcus aureus (hereinafter referred to as "MRSA") has multi-drug resistance and is associated with high morbidity and mortality. It is one of the important pathogenic bacteria of nosocomial infection and community infection, which brings great difficulties to clinical treatment. MRSA is resistant to the vast majority of antibacterial drugs, and even vancomycin, which is the most effective clinical anti-drug-resistant bacteria, has begun to develop drug resistance. Once MRSA develops to the extent of universal resistan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48A61P31/04A61K127/00A61K135/00
Inventor 王永刚刘博宇苏薇薇姜傥李泮霖刘赛娟
Owner HUACHENG PHARMA FACTORY GAUNGZHOU
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