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A kind of material and application based on natural diatom shell and phycobiliprotein

A technology of phycobiliprotein and diatom algae, which is applied in the direction of wave energy or particle radiation treatment materials, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem that the structure of phycocyanin is not very stable, Weak photosensitive effect, limited application and other issues, to achieve the effect of stable structure, convenient material acquisition and strong stability

Active Publication Date: 2021-07-16
山东北游生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the structure of phycocyanin is not very stable, and the photosensitization effect is weak, so its application as a photosensitizer is greatly limited.

Method used

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  • A kind of material and application based on natural diatom shell and phycobiliprotein
  • A kind of material and application based on natural diatom shell and phycobiliprotein
  • A kind of material and application based on natural diatom shell and phycobiliprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A material preparation method based on natural diatom shells and phycocyanin, comprising the steps of:

[0043](1) Prepare f / 2 seawater medium and add 30 mg / L sodium silicate to adjust the pH to 7.8-8.0. Inoculate the diatom algae species of the genus Nanofrustulum, set the culture temperature of the light incubator at 25°C, 12h, 600Lux continuous light, 12h dark treatment, and regularly collect well-growing diatoms by precipitation method (diatom optical microscope picture See figure 1 ).

[0044] (2) Settling the harvested diatom algae in a beaker for 6 hours, absorbing the supernatant, adding 10 mL of distilled water and blowing, then standing for 4 hours, absorbing the supernatant, adding 100 mL of distilled water, and standing at room temperature for four to five days, Aspirate the supernatant, add 35mL hydrogen peroxide, and treat in a water bath at 90°C for 4 hours in the dark; the pure silicon shell obtained by removing the organic matter is obtained (the scan...

Embodiment 2

[0053] A material preparation method based on natural diatom shells and phycocyanin, comprising the steps of:

[0054] (1) Prepare f / 2 seawater medium and add 30 mg / L sodium silicate to adjust the pH to 7.8-8.0. Inoculate the diatom species and set the culture temperature of the light incubator at 25°C, 12h, 600Lux continuous light, 12h dark treatment, and regularly collect diatoms by precipitation method.

[0055] (2) Settling the harvested diatom algae in a beaker for 6 hours, absorbing the supernatant, adding 10 mL of distilled water and blowing, then standing for 4 hours, absorbing the supernatant, adding 100 mL of distilled water, and standing at room temperature for four to five days, Aspirate the supernatant, add 35mL hydrogen peroxide, and treat in a 90°C water bath in the dark for 4 hours; the organic matter is removed to obtain a pure silicon shell.

[0056] (3) Piranha solution (H 2 o 2 :H 2 SO 4 =1:6 mixing) at 80°C for 30 minutes, and washed twice with deioni...

Embodiment 3

[0064] A material preparation method based on natural diatom shells and phycocyanin, comprising the steps of:

[0065] (1) Prepare f / 2 seawater medium and add 30 mg / L sodium silicate to adjust the pH to 7.8-8.0. Inoculate the diatom species and set the culture temperature of the light incubator at 25°C, 12h, 600Lux continuous light, 12h dark treatment, and regularly collect diatoms by precipitation method.

[0066] (2) Settling the harvested diatom algae in a beaker for 10 hours, absorbing the supernatant, adding 100 mL of distilled water and blowing, then standing for 6 hours, absorbing the supernatant, adding 200 mL of distilled water, and standing at room temperature for four to five days, Aspirate the supernatant, add 85mL hydrogen peroxide, and treat in a water bath at 80°C in the dark for 4 hours; the organic matter is removed to obtain a pure silicon shell.

[0067] (3) Piranha solution (H 2 o 2 :H 2 SO 4 =1:8 mixing) at 80°C for 50 minutes, and washed twice with d...

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Abstract

The invention discloses a material based on natural diatom shells and phycobiliproteins and its application. The invention removes the organic matter contained in the shells of cultivated diatoms, and then utilizes APTES (3-aminopropyltrimethoxysilane) to make The silicon shell is alkylated, and then the alkylated silicon shell is reacted with the phycobiliprotein through BS3 (sulfosuccinimidyl suberate) to form a covalent connection to achieve the purpose of immobilizing the phycobiliprotein in the diatom shell . The diatom shell used is easy to obtain, relatively low in price and has high biocompatibility, stability, reaction inertia and certain light effect, so that the connected phycobiliprotein structure is more stable and has a sensitizing effect .

Description

technical field [0001] The invention relates to the technical field of new materials, in particular to a material based on natural diatom shell and phycobiliprotein and its application. Background technique [0002] Phycobiliprotein is a light-harvesting pigment protein with covalently linked open-chain tetrapyrrole structure phycobilin present in cyanobacteria, red algae, cryptophyga and a few dinoflagellates; it can efficiently transfer the captured light energy to the photoreaction The center is a class of oligomer water-soluble pigment proteins, mainly including phycocyanin and phycoerythrin. Phycocyanin is the most ubiquitous phycobiliprotein and can be observed in almost all phycobiliprotein-containing organisms. Most abundant in most cyanobacterial species that grow in natural environments. These blue or blue-purple phycobiliproteins can absorb visible light from 580nm to 630nm, and emit red fluorescence intensively in the range of 635nm-645nm. Phycoerythrin is als...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/69A61P35/00
CPCA61K41/0057A61K47/6923A61P35/00
Inventor 赵华伟蒲洋魏梦娇李文军秦松
Owner 山东北游生物科技有限公司
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