High efficiency liquid chromatography detecting method of erythromycin in cosmetic

Abstract

The invention relates to a high efficiency liquid phase chromatogram method for detecting erythromycin in cosmetics, which is characterized in that: a C8 column or a C18 column with octadecylsilane chemically bonded silica as fillers is adopted; an ultraviolet detector or a diode array detector is used to conduct detection at the position of 190-380nm, ammonium dihydrogen phosphate solution-acetonitrile buffer solution or ammonium dihydrogen phosphate solution-methanol buffer solution is used as mobile phase. The sample is extracted by dried methyl alcohol or acetonitrile, and then extracted by ultrasonic wave for 20 min, and then centrifuged for 5 min at the speed of 12000rpm / min; supernatant solution is taken out as the liquid for test, and can be filtered for later use by means of 0.45 Mu m filter membrane if necessary; qualitative analysis is conducted according the keeping time of the sample and the spectrogram, and quantitative analysis can be performed according to the peak area. The invention has the advantages of high definition and sensitivity, good reproducibility and selectivity, and simple and quick pre-processing method of sample; therefore; the detection method is applicable to the microanalysis of erythromycin in cosmetics.

Description

technical field [0001] The invention is a new technology for detecting erythromycin in cosmetics by using high-performance liquid chromatography, and is especially suitable for qualitative and quantitative analysis of trace antibiotic erythromycin in cosmetics. Background technique [0002] Erythromycin (Erythromycin) is a kind of basic antibiotic, is macrolide antibacterial drug, is widely used in medicine, feedstuff, and cosmetic as a kind of broad-spectrum high-efficiency antibacterial drug. Erythromycin has a strong inhibitory effect on Gram-positive bacteria, such as Staphylococcus, Streptococcus pyogenes, and Streptococcus viridans; it has a strong inhibitory effect on Gram-negative bacteria, such as Neisseria gonorrhoeae, Helicobacter, Bordetella pertussis, and Haemophilus influenzae It also has a considerable inhibitory effect; in addition, it has inhibitory effects on mycoplasma, actinomycetes, spirochetes, rickettsia, chlamydia, a small number of mycobacteria and a...

AI Technical Summary

Problems solved by technology

Antibiotic microbial assay is a method for detecting erythromycin in drugs stipulated in "Chinese Pharmacopoeia" (2000 edition). This method has a long cycle, cumbersome operation, many influencing factors, and low precision.

Ultraviolet spectrophotometry is a relatively classic method, but its sensitivity is not high, and its specificity is poor, so it is not suitable for the analysis of complex samples.

Thin-layer chromatography has high sensitivity and good selectivity, but poor repeatability and cumbersome detection steps

Although chemiluminescence analysis and second-order derivative differential pulse polarography have high detection sensitivity, there are factors such as expensive instruments and difficult popularization of equipment.

High performance liquid chromatography is currently the most commonly used method for detecting erythromycin drugs, but it is only limited to the content detection of various erythromycin drug preparations, and the detection of erythromycin content in cosmetics, feed, and meat has not yet been performed. Relevant standards or methods have been introduced. Since the drug components are less, the matrix is ​​simple, the interference factors are less, and the sample pretreatment is easy to operate, and the loss of the target detection substance is small, so the content of erythromycin drug is easy to detect

Different from medicines, cosmetics have various types, complex substrates, large differences in the physical and chemical properties of the ingredients, and many interfering factors. In particular, erythromycin is added to cosmetics as a banned ingredient in cosmetics, and its content is low. The various methods mentioned above It is difficult to accurately detect trace amounts of erythromycin in cosmetics, and the method of using high performance liquid chromatography to detect erythromycin in cosmetics has not been reported in the literature

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