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Improved method for purifying erythromycin A

A technology for erythromycin and fermentation broth, which is applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the method for removing impurities such as pigments and proteins, and does not consider the effect of removing impurities in fermentation broth. , low elution rate of erythromycin, etc., to achieve the effect of high yield, improved yield and improved quality

Inactive Publication Date: 2011-09-14
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

[0004] Existing documents related to the method of purifying erythromycin A from fermentation broth by resin adsorption method include: Chinese Journal of Antibiotics 2007, 32 (5). 284-286, 315 and patent document CN 101367855, the technical scheme disclosed by it As can be seen, the main defects of the prior art are: (1) the elution rate of the 1st BV erythromycin is relatively low, (2) the method for removing impurities such as pigment and protein in the fermentation broth is not involved (so the quality of the final product will be affected) , (3) The regeneration of the adsorption column does not consider the removal effect of impurities in the fermentation broth, and the regeneration effect is not good

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Embodiment

[0027] (1) Resin adsorption

[0028] After putting the erythromycin fermentation liquid into the tank, it was filtered through a 50nm ceramic membrane. After adjusting the pH of the obtained filtrate to 9.30, it was passed into a glass chromatography column equipped with macroporous adsorption resin SEPABEADS SP825 for resin adsorption. 63.7% of the volume.

[0029] (2) Resin washing

[0030] For the resin that has been adsorbed in the previous step, feed the borax-NaOH buffer solution with a pH value of 9.78, and wash the resin at 40°C. Stop washing when the pH of the instantaneous effluent is 9.78, and the loss rate of erythromycin during the washing process is 0.92 %.

[0031] (3) Resin elution

[0032] For the resin washed in the previous step, first use 1BV of deionized water to pass through the column, and then pass through butyl acetate for resin elution. The chemical potency of erythromycin in the 1BV eluent is 70062u / mL, and the elution rate is 91.6 % (the elution...

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Abstract

The invention relates to method for purifying erythromycin A from fermented liquid by adopting a resin adsorption method, which includes the following steps: (1) adsorption, (2) washing of impurities, (3) desorption, (4) salifying of eluent and the like. The method overcomes the defects of lower elution rate of 1BV erythromycin, more impurity contents in products and the like in the existing resin adsorption and separation technology. In addition, the invention also provides a resin regeneration method which includes the following main steps: mixed solution consisting of 0.1 mol / L-1.0 mol / L NaOH aqueous solution and a water soluble or partially water soluble organic solvent is adopted for washing resin to be regenerated, so as to solve the problem of poor resin regeneration effect in the prior art.

Description

technical field [0001] The invention relates to a method for purifying erythromycin A, in particular to a method for purifying erythromycin A from fermentation broth by adopting a macroporous resin adsorption method. Background technique [0002] Erythromycin (EM for short) is a macrolide broad-spectrum antibiotic fermented from Streptomyces erythromycin, and it is one of the main antibiotic products at present. There are many different isomers such as erythromycin A, B and C in erythromycin, and their chemical properties and structures are very similar, but among them, erythromycin A has strong antibacterial activity and low toxicity. The main antibacterial active ingredient of mycin. [0003] In the extraction and purification of erythromycin A, compared with the traditional solvent extraction method, the resin adsorption method has the advantages of less solvent loss, simple operation, and less environmental pollution, and has been paid more and more attention. [0004]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/08C07H1/06
Inventor 朱家文朱晟陈葵武斌纪利俊吴艳阳
Owner EAST CHINA UNIV OF SCI & TECH
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