Compound microbial fertilizer for preventing and treating take-all disease of wheat and preparation method of compound microbial fertilizer
A technology for complex microbial bacteria and wheat take-all, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the problem of single mode of action, unreachable production and use, and unstable control effect and other problems, to achieve the effect of simple use, low cost, and enhanced disease prevention effect
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Embodiment 1
[0050] A compound microbial bacterial fertilizer for preventing and treating take-all of wheat, the raw material ratio of the microbial bacterial fertilizer is: 30 parts of Streptomyces mitogenus powder, 20 parts of Streptomyces albicans powder, 30 parts of Bacillus flexus, Ayerbordella 20 parts of Bacillus bacteria powder, 30 parts of Trichoderma brevis compactus bacteria powder.
[0051] In order to achieve the above object, a kind of preparation method of the composite microbial bacterial manure of preventing and treating wheat take-all, comprises the following steps:
[0052] Step 1, the preparation of Streptomyces parvulus bacteria powder
[0053]Take out the preservation tube of Streptomyces parenchyma, scratch the plate with Gao's No. 1 solid medium for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and inoculate it into an eggplant bottle with 150 ml of Gaoshi No. 1 solid medium, and culture it in an incubator at 30°C for 6-8 days. When...
Embodiment 2
[0085] Field plot test of compound microbial bacterial fertilizer prepared in Example 1 to control wheat take-all
[0086]Adopt random block analysis, 3 repetitions, each plot area is 2m , plant 5 rows, each row sows 2.5g seed;
[0087] Test group 1: Inoculate 25g wheat take-all pathogen inoculum per m2, and pour 1000mL of 200-fold dilution of compound microbial fertilizer at the same time; Test group 2: Do not inoculate pathogenic bacteria, only water compound microbial fertilizer; Corrosion bacteria, no compound microbial fertilizer; healthy control group: no pathogenic bacteria, no compound microbial fertilizer. At the wheat seedling stage, jointing stage and milk maturity stage, the number of affected plants was investigated, and the incidence rate and control effect were calculated. At the same time, the number of spikes, grains per spike, white spikes, calculated yield, thousand-grain weight, etc. were investigated during the milk maturity stage of wheat.
[0088] Test...
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