Compound microbial fertilizer for preventing and treating take-all disease of wheat and preparation method of compound microbial fertilizer

A technology for complex microbial bacteria and wheat take-all, applied in the direction of microorganism-based methods, biochemical equipment and methods, botany equipment and methods, etc., can solve the problem of single mode of action, unreachable production and use, and unstable control effect and other problems, to achieve the effect of simple use, low cost, and enhanced disease prevention effect

Inactive Publication Date: 2016-12-21
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0005] Due to the diversity and simultaneous evolution of pathogenic bacteria, the existing single antagonistic strains cannot achieve ideal control effects due to their single mode of action, uns

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A compound microbial bacterial fertilizer for preventing and treating take-all of wheat, the raw material ratio of the microbial bacterial fertilizer is: 30 parts of Streptomyces mitogenus powder, 20 parts of Streptomyces albicans powder, 30 parts of Bacillus flexus, Ayerbordella 20 parts of Bacillus bacteria powder, 30 parts of Trichoderma brevis compactus bacteria powder.

[0051] In order to achieve the above object, a kind of preparation method of the composite microbial bacterial manure of preventing and treating wheat take-all, comprises the following steps:

[0052] Step 1, the preparation of Streptomyces parvulus bacteria powder

[0053]Take out the preservation tube of Streptomyces parenchyma, scratch the plate with Gao's No. 1 solid medium for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and inoculate it into an eggplant bottle with 150 ml of Gaoshi No. 1 solid medium, and culture it in an incubator at 30°C for 6-8 days. When...

Embodiment 2

[0085] Field plot test of compound microbial bacterial fertilizer prepared in Example 1 to control wheat take-all

[0086]Adopt random block analysis, 3 repetitions, each plot area is 2m , plant 5 rows, each row sows 2.5g seed;

[0087] Test group 1: Inoculate 25g wheat take-all pathogen inoculum per m2, and pour 1000mL of 200-fold dilution of compound microbial fertilizer at the same time; Test group 2: Do not inoculate pathogenic bacteria, only water compound microbial fertilizer; Corrosion bacteria, no compound microbial fertilizer; healthy control group: no pathogenic bacteria, no compound microbial fertilizer. At the wheat seedling stage, jointing stage and milk maturity stage, the number of affected plants was investigated, and the incidence rate and control effect were calculated. At the same time, the number of spikes, grains per spike, white spikes, calculated yield, thousand-grain weight, etc. were investigated during the milk maturity stage of wheat.

[0088] Test...

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PUM

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Abstract

The invention discloses a compound microbial fertilizer for preventing and treating take-all disease of wheat and a preparation method of the compound microbial fertilizer. The compound microbial fertilizer consists of the following raw materials: streptomyces catenulae, streptomyces albolongus, bacillus flexus, bacillus aryabhattai and trichoderma brevicompactum. The strains of the invention, on the basis of the comprehensive utilization of microbial inter-species or intra-species antibiosis, competition, hyperparasitism and bacteriolysis effects or by inducing the wheat to generate disease resistance by virtue of secondary metabolites, can generate various antibiotics, such as proceomycin, neomycin E, catenulin, diparomomycin and the like, so as to inhibit pathogenic bacteria of the take-all disease of wheat; the five strains adopted by the invention are directly separated from soil and have a rhizosphere growth promoting effect; the strains can promote reproduction and growth on crop surfaces or in plants or soil, and meanwhile, the strains can take an effect on nitrogen fixation and can promote the directional secretion of some secondary metabolites from plant rhizosphere; and the strains can enhance nutrition absorption of plants and stimulate plant growth, so that the effects of the fertilizer are achieved.

Description

technical field [0001] The invention belongs to the technical field of agricultural biological control and microbial fertilizer, and in particular relates to a compound microbial fertilizer for preventing and controlling wheat take-all and a preparation method thereof. Background technique [0002] Wheat take-all disease is a soil-borne and destructive disease caused by the infection of Ascomycota acrotheca genus, which has caused serious losses to humans. The disease is susceptible throughout the wheat growth period. After the onset at the seedling stage, the primary roots and underground stems of wheat seedlings turn dark brown, and there are many lesions on the secondary roots. In severe cases, the lesions are connected together, causing the entire root system to turn black and die. Short, with yellowish leaves on the ground, easy to break off from the rhizome. There are no obvious symptoms in the aboveground part of the millet division stage, only the seriously disease...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/04A01N63/00A01P3/00C12R1/465C12R1/07C12R1/885
CPCA01N63/00A01N63/30C12N1/14C12N1/20A01N2300/00
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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