A type of honey with medicinal value and its preparation method

Abstract

This invention discloses a medicinal honey and its preparation method. The steps are as follows: extracting the effective components from traditional Chinese medicine to obtain an extract, mixing it with pure honey to obtain a medicinal mixed honey, feeding live bees for a second brewing process for 48-120 hours, and then separating and purifying the honey after harvesting to obtain the finished product. The preferred traditional Chinese medicines are selected from nine kinds of food and medicinal materials, such as honeysuckle. The extract is obtained using methods such as water extraction. The mixing ratio, stirring parameters, brewing temperature and humidity, and harvesting and purification conditions are optimized, and additional low-temperature sterilization can be performed. This invention achieves deep integration of traditional Chinese medicine and honey through a second brewing process by bees, improving absorption and utilization rates. It has high safety, excellent taste, good stability, a simple process, is easy to scale up, and has broad application prospects.

Description

Technical Field

[0001] This invention belongs to the field of honey processing and traditional Chinese medicine application technology, specifically relating to a honey with medicinal value and its preparation method. Background Technology

[0002] Honey, a traditional food and medicine, possesses properties such as nourishing the body, relieving dryness, alleviating pain, and detoxifying. It is widely used in the food, health product, and pharmaceutical industries. Traditional honey is primarily made by bees collecting nectar from plants, and its main nutritional components are glucose, fructose, vitamins, and minerals. Its medicinal effects are relatively limited, making it difficult to meet people's needs for targeted health maintenance and adjunctive treatment.

[0003] Currently, while there are honey products on the market with added traditional Chinese medicine ingredients, most are prepared by directly mixing powdered or crude extracts of traditional Chinese medicine with honey. This method suffers from uneven dispersion of the effective components of the traditional Chinese medicine, low absorption and utilization rate by the human body, unpleasant taste, and is prone to sedimentation and spoilage, failing to fully realize the synergistic medicinal effects of traditional Chinese medicine and honey. Furthermore, existing technologies lack processes that utilize the bees' own brewing behavior and internal enzyme systems to transform the effective components of traditional Chinese medicine, resulting in poor integration of the traditional Chinese medicine components with honey, making it difficult to guarantee the medicinal value and stability of the product.

[0004] Therefore, developing a type of honey with medicinal value that can deeply integrate the effective components of traditional Chinese medicine with honey, has a high absorption and utilization rate, significant medicinal efficacy, and good stability, as well as its preparation method, has become an urgent technical problem to be solved in this field. Summary of the Invention

[0005] In view of the shortcomings of existing technologies, such as the limited medicinal effects of honey, poor integration of honey products with added Chinese medicine, and low absorption and utilization rates, this invention provides a honey with medicinal value and its preparation method. By mixing the effective components of Chinese herbal medicine with honey, and then using the foraging and brewing behavior of bees and their internal enzyme system for secondary brewing, the effective components of Chinese herbal medicine are deeply integrated with honey, thereby improving the medicinal value and absorption and utilization rate of the product, while ensuring the stability and taste of the product.

[0006] To achieve the above objectives, the present invention employs the following technical means: A method for preparing honey with medicinal value includes the following steps: (a) Extracting active ingredients from raw materials of traditional Chinese medicine to obtain extracts; (b) Mix the extract from step (a) with pure honey until homogeneous to obtain a medicinal mixed honey; (c) Feed the medicated honey from step (b) to live bees and use the bees’ feeding and brewing behavior and their enzyme system to carry out secondary brewing for 48-120 hours. (d) Harvest the honey produced by bees, and then separate and purify it to obtain the finished product.

[0007] Preferably, the Chinese herbal medicines mentioned in step (a) are selected from one or more of the following: honeysuckle, astragalus, angelica, licorice, wolfberry, ophiopogon japonicus, isatis root, forsythia, and kudzu root.

[0008] Preferably, the extract in step (a) is extracted by water extraction, alcohol extraction, or water extraction and alcohol precipitation.

[0009] Preferably, in step (b), the weight ratio of the extract to pure honey is 1:5 to 1:50.

[0010] Preferably, the mixing process in step (b) adopts a stirring and homogenization method, with a stirring speed of 100-300 r / min and a stirring time of 10-30 minutes.

[0011] Preferably, the bees mentioned in step (c) are Italian honeybees or Chinese honeybees.

[0012] Preferably, during the secondary brewing process in step (c), the temperature inside the beehive is maintained at 33-36°C and the relative humidity at 40%-75%.

[0013] Preferably, in step (d), honey is harvested by centrifugation, filtration or pressing. Filtration uses a 200-300 mesh filter, and purification is carried out by static precipitation at 4-8℃ for 12-24 hours.

[0014] Preferably, in step (d), the harvested honey is further subjected to low-temperature sterilization treatment at a temperature of 40-50°C for 10-15 minutes.

[0015] A type of honey with medicinal value obtained by the preparation method described above.

[0016] The present invention has the following beneficial effects: 1. This invention innovatively adopts a two-stage brewing process using bees, utilizing the bees' foraging and brewing behaviors and their internal enzyme systems to deeply integrate Chinese herbal extracts with honey, thereby transforming the effective components of the Chinese medicine and making them easier for the human body to absorb and utilize. This solves the problems of poor integration and low absorption and utilization rate of traditional Chinese medicine-added honey products.

[0017] 2. This invention selects Chinese herbal medicines that are both food and medicine, which are highly safe. Different medicinal materials can be combined according to needs to achieve targeted medicinal effects, enriching the product types and medicinal value of honey and meeting the health care and adjunctive treatment needs of different groups of people.

[0018] 3. By optimizing the mixing ratio of extract and honey, and parameters such as temperature and humidity during secondary brewing, this invention ensures that the product has a mellow taste, no off-flavors, good stability, and is not prone to sedimentation or spoilage, thus extending the product's shelf life.

[0019] 4. The preparation process of this invention is simple and convenient, with low production cost. It does not require complex equipment, is easy to scale up, and has broad market application prospects. Detailed Implementation

[0020] The technical solution of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] A method for preparing honey with medicinal value includes the following steps: (a) Extracting active ingredients from raw materials of traditional Chinese medicine to obtain extracts; (b) Mix the extract from step (a) with pure honey until homogeneous to obtain a medicinal mixed honey; (c) Feed the medicated honey from step (b) to live bees and use the bees’ feeding and brewing behavior and their enzyme system to carry out secondary brewing for 48-120 hours. (d) Harvest the honey produced by bees, and then separate and purify it to obtain the finished product.

[0022] Furthermore, the Chinese herbal medicines mentioned in step (a) are selected from one or more of the following: honeysuckle, astragalus, angelica, licorice, wolfberry, ophiopogon japonicus, isatis root, forsythia, and kudzu root. All of these Chinese herbal medicines are food-grade ingredients with high safety, and their effects are complementary. They can be used in combination according to needs. For example, honeysuckle combined with forsythia can enhance the heat-clearing and detoxifying effects, while astragalus combined with angelica can improve the qi-tonifying and blood-nourishing effects.

[0023] Furthermore, the extract described in step (a) can be extracted using water extraction, alcohol extraction, or water extraction with alcohol precipitation, with water extraction with alcohol precipitation being preferred. This method can efficiently extract the active ingredients from traditional Chinese medicine, remove impurities, and improve the purity of the extract, while also ensuring good compatibility with honey and preventing excessive impurities from affecting the product's taste and stability. The specific extraction parameters can be adjusted according to the characteristics of different traditional Chinese medicines to ensure the full extraction of effective components.

[0024] Furthermore, in step (b), the weight ratio of the extract to pure honey is 1:5 to 1:50. This ratio range has been verified through extensive testing. It ensures that the product has significant medicinal efficacy while avoiding the negative effects of excessive extract addition, such as poor honey taste, off-flavors, or sedimentation. It also ensures that the extract is evenly dispersed in the honey, laying the foundation for subsequent secondary honey production by the bees. The preferred weight ratio is 1:10 to 1:30, at which point the integration of the active ingredients of the traditional Chinese medicine with the honey is optimal, achieving a balance between taste and medicinal efficacy.

[0025] Furthermore, the mixing process in step (b) can be carried out by stirring and homogenizing at a speed of 100-300 r / min for 10-30 minutes to ensure that the extract and pure honey are fully mixed without obvious layering or sedimentation.

[0026] Furthermore, the bees mentioned in step (c) are either Italian honeybees or Chinese honeybees. Both types of bees have strong foraging and brewing abilities, and their internal enzyme systems are rich, enabling them to effectively perform secondary brewing on honey containing medicinal ingredients, transforming the effective components of traditional Chinese medicine and improving their absorption and utilization rate. Among them, Chinese honeybees are highly adaptable and have good tolerance to extracts of traditional Chinese medicine, producing honey with a richer taste and a higher degree of integration of medicinal components, making them the preferred bee species.

[0027] Furthermore, during the secondary brewing process in step (c), the temperature inside the beehive is maintained at 33-36℃, and the relative humidity at 40%-75%. This temperature and humidity range is the optimal environment for bees to brew honey, ensuring normal physiological activities and enzyme activity, promoting the full reaction and fusion of the effective components of traditional Chinese medicine with the nutrients in the honey, shortening the brewing cycle, and ensuring product stability. If the temperature is too high, it will lead to the loss of nutrients in the honey and a decrease in enzyme activity; if the temperature is too low or the humidity is too high / too low, it will affect the bees' brewing efficiency, resulting in a decline in product quality.

[0028] Furthermore, during the secondary brewing process in step (c), the remaining amount of medicated mixed honey in the hive should be checked regularly and replenished in a timely manner to ensure that the bees have sufficient food for brewing. At the same time, it is necessary to avoid the bees collecting nectar from external flowers due to insufficient food, which would affect the medicinal purity of the product.

[0029] Furthermore, in step (d), honey is harvested using centrifugation, filtration, or pressing. Among these methods, centrifugation is the preferred method due to its high harvesting efficiency and ability to retain the active ingredients and effective components of traditional Chinese medicine in the honey to the greatest extent. Filtration can be carried out using a 200-300 mesh filter to remove impurities such as beeswax and bee carcasses from the honey. The purification process can employ a low-temperature static sedimentation method, with a settling temperature of 4-8℃ and a settling time of 12-24 hours, to further remove impurities and improve product purity.

[0030] Furthermore, the harvested honey in step (d) can also undergo low-temperature sterilization treatment at a temperature of 40-50℃ for 10-15 minutes. This kills harmful microorganisms while avoiding high temperatures that could damage the active ingredients in the honey and traditional Chinese medicine, thus ensuring the safety and medicinal efficacy of the product.

[0031] This invention also provides a honey with medicinal value obtained by any of the above preparation methods. This honey not only retains the nutritional components and taste of traditional honey, but also incorporates the effective components of traditional Chinese medicine, possessing one or more medicinal effects such as clearing heat and detoxifying, replenishing qi and blood, promoting body fluid production and moistening the lungs, and antibacterial and anti-inflammatory properties. Furthermore, the effective components of the traditional Chinese medicine are more easily absorbed and utilized by the human body after being processed twice by bees. The product has good stability, no obvious sediment or off-odor, and can be widely used in the fields of food, health products, and adjuvant therapy. Example

[0032] A method for preparing honey with medicinal value includes the following steps: (a) Honeysuckle and forsythia were selected as raw materials for traditional Chinese medicine. The effective components were extracted by water extraction and alcohol precipitation: Honeysuckle and forsythia were crushed and 8 times the amount of deionized water were added. The mixture was boiled and extracted twice, each time for 1.5 hours. The two extracts were combined and concentrated under reduced pressure to a concentration with a relative density of 1.1-1.2. Then, 95% ethanol was slowly added to make the ethanol volume fraction reach 70%. The mixture was allowed to stand for 24 hours, filtered, and the filtrate was collected and the ethanol was recovered by vacuum distillation to obtain the traditional Chinese medicine extract. (b) The extract obtained in step (a) is mixed with pure acacia honey at a weight ratio of 1:10, and stirred at 200 r / min for 20 minutes until the mixture is homogeneous, thus obtaining a medicinal mixed honey. (c) Feed the Chinese honey containing the drug to the honeybees, maintain the temperature in the hive at 35°C and the relative humidity at 60%, and allow the bees to make a second batch of honey for 72 hours, during which time the honey containing the drug is replenished regularly. (d) Honey produced by bees is harvested by centrifugation, filtered through a 250-mesh filter to remove impurities, then purified by standing at 4°C for 18 hours, and then sterilized at 45°C for 12 minutes to obtain honey with medicinal value.

[0033] The honey prepared in this embodiment combines the heat-clearing and detoxifying effects of honeysuckle and forsythia. It has a mellow taste, no off-flavor, and high absorption and utilization rate of the effective components of traditional Chinese medicine. It can be used to help relieve symptoms such as sore throat and wind-heat cold. Example

[0034] A method for preparing honey with medicinal value includes the following steps: (a) Astragalus membranaceus, Angelica sinensis and Lycium barbarum were selected as raw materials of traditional Chinese medicine. The effective components were extracted by water extraction and alcohol precipitation: Astragalus membranaceus, Angelica sinensis and Lycium barbarum were crushed and 10 times the amount of deionized water were added. The mixture was boiled and extracted 3 times, 1 hour each time. The three extracts were combined and concentrated under reduced pressure to a concentration with a relative density of 1.15-1.25. Then 95% ethanol was slowly added to make the ethanol volume fraction reach 65%. The mixture was allowed to stand for 20 hours, filtered, and the filtrate was collected and the ethanol was recovered by vacuum distillation to obtain the traditional Chinese medicine extract. (b) The extract obtained in step (a) is mixed with pure jujube honey at a weight ratio of 1:20, and stirred at 250 r / min for 15 minutes until the mixture is homogeneous, thus obtaining a medicinal mixed honey. (c) Feed the Italian honey containing the drug to the bees, maintain the temperature in the hive at 34°C and the relative humidity at 50%, and allow the bees to make a second batch of honey for 96 hours, during which time the honey containing the drug is replenished regularly. (d) Honey produced by bees is harvested by pressing, filtered through a 300-mesh filter to remove impurities, then purified by standing at 6°C for 24 hours, and then sterilized at 40°C for 15 minutes to obtain honey with medicinal value.

[0035] The honey prepared in this embodiment combines the qi-tonifying and blood-nourishing effects of astragalus and angelica with the liver- and kidney-tonifying effects of wolfberry. It has a sweet taste and is rich in nutrients, and can be used to help improve symptoms such as qi and blood deficiency and liver and kidney deficiency. Example

[0036] A method for preparing honey with medicinal value includes the following steps: (a) Licorice, Ophiopogon japonicus and kudzu root were selected as raw materials of traditional Chinese medicine. The effective components were extracted by water extraction and alcohol precipitation: Licorice, Ophiopogon japonicus and kudzu root were crushed and 9 times the amount of deionized water were added. The mixture was boiled and extracted twice, each time for 2 hours. The two extracts were combined and concentrated under reduced pressure to a concentration with a relative density of 1.2-1.3. Then 95% ethanol was slowly added to make the ethanol volume fraction reach 75%. The mixture was allowed to stand for 22 hours, filtered, and the filtrate was taken and the ethanol was recovered by vacuum distillation to obtain the traditional Chinese medicine extract. (b) The extract obtained in step (a) is mixed with pure motherwort honey at a weight ratio of 1:30, and stirred at 150 r / min for 25 minutes until the mixture is homogeneous, thus obtaining a medicinal mixed honey. (c) Feed the Chinese honey containing the drug to the honeybees, maintain the temperature in the hive at 36°C and the relative humidity at 70%, and allow the bees to make a second batch of honey. The honey will be made for 48 hours, during which time the honey containing the drug will be added regularly. (d) Honey produced by bees is harvested by filtration, filtered through a 200-mesh filter to remove impurities, then purified by standing at 8°C for 12 hours, and then sterilized at 50°C for 10 minutes to obtain honey with medicinal value.

[0037] The honey prepared in this embodiment combines the harmonizing effects of licorice, the moisturizing and lung-nourishing effects of Ophiopogon japonicus, and the muscle-relieving and fever-reducing effects of kudzu root. It has a mild and smooth taste and can be used to help relieve symptoms such as dry mouth and tongue, and fever caused by external pathogens.

[0038] mouse experiment To verify the safety and efficacy of the honey with medicinal value prepared by this invention, acute toxicity tests and efficacy verification tests were conducted on mice. The specific test protocols are as follows. All tests were conducted in accordance with the basic requirements for acute oral toxicity tests in the food standard GB15193.3-2014 and related animal testing standards.

[0039] Test materials 1. Test Samples: The honey with medicinal value prepared in Examples 1, 2 and 3 of this invention are respectively designated as Sample 1 (heat-clearing and detoxifying type), Sample 2 (qi-tonifying and blood-nourishing type), and Sample 3 (fluid-generating and lung-moistening type); ordinary pure honey (without Chinese medicine ingredients) is used as a blank control group sample; the corresponding Chinese herbal extract (not processed by bees) and ordinary honey mixture is used as a control group sample (designated as Control Sample 1, Control Sample 2 and Control Sample 3, corresponding to the Chinese herbal ratio of Sample 1, Sample 2 and Sample 3 respectively).

[0040] 2. Experimental animals: Healthy Kunming mice, weighing 18-22g, half male and half female, totaling 300 mice. Before the experiment, they were allowed to acclimatize to the experimental environment for 3-5 days and underwent quarantine observation. They were used in the experiment after being confirmed to be free of disease and in good mental condition. The feeding conditions met national standards. They were fed basic feed, had free access to water, and were housed in separate cages according to sex. The number of mice in each cage should be such that it does not affect the free movement of the animals and the observation of their physical signs.

[0041] 3. Experimental equipment: Electronic balance, gavage needle, mouse cage, constant temperature and humidity incubator, centrifuge, enzyme-linked immunosorbent assay reader, dissection instruments, etc., are all sterilized before use.

[0042] 4. Test reagents: physiological saline, HCoV-229E virus solution, IL-1β detection kit, SOD detection kit, MDA detection kit, CD4+T / CD8+T cell detection kit, etc., are all commercially available qualified products.

[0043] Experiment 1: Acute Toxicity Test 1. Experimental grouping: Sixty mice were randomly divided into 6 groups of 10 each, with 5 males and 5 females. These groups were: Sample 1, Sample 2, Sample 3, Blank control group, Solvent control group (physiological saline), and Positive control group (known toxic drug).

[0044] 2. Preparation and administration of test substances: Each test sample was dissolved in physiological saline to prepare a suspension. Each group of mice was administered the suspension orally once at a gavage volume of 20 mL / kg body weight. The blank control group was administered an equal volume of ordinary honey suspension, the solvent control group was administered an equal volume of physiological saline, and the positive control group was administered an equal volume of known toxic drug suspension. Mice were fasted for 4-6 hours before the experiment but allowed free access to water. They were fasted for another 1-2 hours after gavage.

[0045] 3. Observation and Recording: After gavage, mice were observed for 14 consecutive days. The mental state, food intake, water intake, activity, fur condition, defecation and urination, and mortality of the mice were observed and recorded daily. The mice were weighed once a week and the weight changes were recorded. After the observation period, the surviving mice were dissected to observe the morphology of major organs such as heart, liver, spleen, lungs, and kidneys to determine whether there was any pathological damage, calculate the median lethal dose, and assess the acute toxicity of the sample.

[0046] 4. Experimental Results: During the 14-day observation period, no mice in sample groups 1, 2, and 3, the blank control group, and the solvent control group died. They were in good spirits, fed and drank normally, were agile, had glossy and smooth fur, normal bowel movements, and showed a steady weight gain. Dissection revealed normal morphology of all major organs, with no pathological damage such as congestion, edema, or necrosis. LD50 was [not specified]. 50 All levels were greater than 5000 mg / kg body weight, classifying it as practically non-toxic. The positive control group mice showed significant toxic reactions, with some dying, and autopsies revealing obvious pathological damage to organs. The experiments demonstrate that the honey prepared according to this invention, possessing medicinal value, is highly safe, has no acute toxicity, and meets food and health product safety requirements.

[0047] Experiment 2: Efficacy Verification Experiment Based on the medicinal efficacy of samples from different embodiments of the present invention, three efficacy verification tests were designed for clearing heat and detoxifying, replenishing qi and nourishing blood, and promoting body fluid and moistening the lungs. Each test set up a corresponding sample group, a control sample group, and a blank control group, with 10 mice in each group, 5 males and 5 females.

[0048] 1. Verification of the efficacy of clearing heat and detoxifying (for sample 1) (1) Model establishment: Except for the blank control group, the other mice were placed in an artificial climate chamber at (37±2)℃ and (95±5)% relative humidity for 4 hours every day for 7 consecutive days. On the 5th day, after being lightly anesthetized with ether, they were injected with 100 TCID. 50 HCoV-229E virus fluid was administered via nasal instillation (50 μL / mouse) to establish a mouse model of lung invasion by damp-heat virus. The blank control group was not subjected to damp-heat stimulation or viral infection, but was treated with physiological saline under the same conditions.

[0049] (2) Administration method: Gavage administration began on the day of infection. Sample 1 group was given sample 1 suspension by gavage, control sample 1 group was given control sample 1 suspension by gavage, and blank control group was given an equal volume of physiological saline by gavage. The administration was once a day, with a gavage volume of 20 mL / kg body weight each time, for 3 consecutive days.

[0050] (3) Detection indicators: After the administration of the drug, observe the general condition of the mice; weigh the mice and lung weight, calculate the lung index and lung index inhibition rate; detect the contents of IL-1β, MDA and SOD in the lung tissue of mice, the contents of PGE2 and cAMP in the hypothalamus, and the ratio of CD4+T / CD8+T cells in peripheral blood; detect the expression level of viral nucleic acid in the lung tissue.

[0051] (4) Experimental results: The mice in the blank control group had normal weight, were active, and showed no abnormal symptoms. The mice in the model group (without drug administration) had reduced weight, were lethargic, had damp fur, poor appetite, significantly increased lung index, significantly increased levels of IL-1β, MDA, and PGE2 and cAMP in lung tissue, significantly decreased SOD content, significantly decreased CD4+T / CD8+T ratio, and high viral nucleic acid expression in lung tissue. Compared with the model group, the mice in sample 1 showed significant improvement in the above symptoms, significantly decreased lung index (lung index inhibition rate reached 25.3%), significantly decreased levels of IL-1β, MDA, and PGE2 and cAMP in lung tissue, significantly increased SOD content, restored CD4+T / CD8+T ratio to normal, and significantly decreased viral nucleic acid expression in lung tissue (P<0.05). The mice in the control sample 1 group also showed improvement in symptoms and various indicators, but the effect was significantly weaker than that of sample 1 group (P<0.05). This indicates that Sample 1 of the present invention has significant effects of clearing heat and detoxifying, anti-inflammatory and antiviral, and that the medicinal effect is better than that of the mixture that has not undergone secondary brewing after being brewed by bees.

[0052] 2. Verification of the efficacy of Qi-tonifying and blood-nourishing medicine (for sample 2) (1) Model establishment: Except for the blank control group, the other mice were used to establish a blood deficiency model by intraperitoneal injection of cyclophosphamide. The injection dose was 80 mg / kg body weight, once a week for 2 consecutive weeks. After successful modeling, the mice showed weight loss, lethargy, dry hair, and significantly reduced red blood cell (RBC) and hemoglobin (Hb) content in the blood routine test.

[0053] (2) Administration method: After successful modeling, the administration was started by gavage. Sample 2 group was administered sample 2 suspension by gavage, control sample 2 group was administered control sample 2 suspension by gavage, and blank control group was administered an equal volume of physiological saline by gavage. The administration was once a day, with a gavage volume of 20 mL / kg body weight each time, for 2 consecutive weeks.

[0054] (3) Detection indicators: After the administration of the drug, observe the mental state and weight changes of the mice; detect the RBC and Hb content in the blood routine test; observe the morphology of the spleen and thymus after dissection and calculate the organ index.

[0055] (4) Experimental Results: The mice in the blank control group were in good spirits and had normal weight. The RBC, Hb content, spleen, and thymus indices were all within the normal range. The mice in the model group were lethargic and had low weight. The RBC, Hb content, spleen, and thymus indices were significantly lower than the normal range. Compared with the model group, the mice in sample 2 showed significantly improved spirits and steady weight gain. The RBC, Hb content, spleen, and thymus indices were significantly increased (P<0.05), approaching the normal range. The indicators in the control sample 2 group also improved, but the increase was significantly lower than that in sample 2 group (P<0.05). This indicates that sample 2 of the present invention has significant qi-tonifying and blood-nourishing effects, can effectively improve the state of qi and blood deficiency, and after being brewed twice by bees, the absorption and utilization rate of effective components is higher, and the efficacy is better.

[0056] 3. Verification of the efficacy of promoting body fluid production and moistening the lungs (for sample 3) (1) Model establishment: Except for the blank control group, the other mice were used to establish a lung dryness model by dry environment combined with ammonia stimulation. The mice were kept in a dry environment with a relative humidity of less than 30%, and ammonia was used to stimulate the nose once a day, 5 μL / mouse each time, for 7 consecutive days. After successful modeling, the mice showed increased water intake, dry mouth and nose, cough, and dry hair.

[0057] (2) Administration method: After successful modeling, the administration was started by gavage. Sample group 3 was administered sample 3 suspension by gavage, control group 3 was administered control sample 3 suspension by gavage, and blank control group was administered an equal volume of physiological saline by gavage. The administration was once a day, with a gavage volume of 20 mL / kg body weight each time, for 5 consecutive days.

[0058] (3) Detection indicators: After the administration of the drug, observe the general condition, cough frequency and water intake of the mice; detect the lung tissue water content, SOD activity and MDA content; dissect and observe the lung tissue morphology to judge the repair of lung dryness damage.

[0059] (4) Experimental results: In the blank control group, mice showed no cough, moist mouth and nose, normal water intake, and normal lung tissue water content, SOD activity, and MDA content. The lung tissue morphology was normal. In the model group, mice coughed frequently, had dry mouth and nose, significantly increased water intake, decreased lung tissue water content, decreased SOD activity, increased MDA content, and showed mild congestion and dryness in the lung tissue. Compared with the model group, mice in sample group 3 showed significantly reduced cough frequency, moist mouth and nose, normal water intake, significantly increased lung tissue water content and SOD activity, and significantly reduced MDA content (P<0.05), indicating significant repair of lung tissue damage. The control sample group 3 also showed improvement in various indicators, but the effect was weaker than that of sample group 3 (P<0.05). This indicates that sample 3 of the present invention has significant effects in promoting body fluid production and moistening the lungs, effectively repairing lung dryness damage, and its efficacy is better than that of the mixture without secondary brewing after being brewed by bees.

[0060] Experimental conclusions Acute toxicity tests on mice showed that the honey prepared by this invention, which has medicinal value, is practically non-toxic, highly safe, and safe for consumption. Pharmacological efficacy verification tests showed that the honey prepared by this invention has significant medicinal effects such as clearing heat and detoxifying, replenishing qi and blood, and promoting body fluids and moistening the lungs. Furthermore, after secondary processing by bees, the absorption and utilization rate of the effective components of traditional Chinese medicine is higher, and the efficacy is significantly better than that of honey mixtures containing traditional Chinese medicine that have not undergone secondary processing. This further proves the rationality and superiority of the preparation process of this invention and provides a scientific experimental basis for the promotion and application of the product.

[0061] The examples provided in this invention are not intended to limit the implementation. Those skilled in the art will recognize that various variations or modifications can be made based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations, and any obvious variations or modifications derived therefrom are still within the scope of this invention.

Claims

1. A method for preparing honey with medicinal value, characterized in that, Includes the following steps: (a) Extracting active ingredients from raw materials of traditional Chinese medicine to obtain extracts; (b) Mix the extract from step (a) with pure honey until homogeneous to obtain a medicinal mixed honey; (c) Feed the medicated honey from step (b) to live bees and use the bees’ feeding and brewing behavior and their enzyme system to carry out secondary brewing for 48-120 hours. (d) Harvest the honey produced by bees, and then separate and purify it to obtain the finished product.

2. The preparation method according to claim 1, characterized in that, The Chinese herbal medicines mentioned in step (a) are selected from one or more of the following: honeysuckle, astragalus, angelica, licorice, wolfberry, ophiopogon japonicus, isatis root, forsythia, and kudzu root.

3. The preparation method according to claim 1, characterized in that, The extract described in step (a) is extracted by water extraction, alcohol extraction, or water extraction and alcohol precipitation.

4. The preparation method according to claim 1, characterized in that, In step (b), the weight ratio of the extract to pure honey is 1:5 to 1:

50.

5. The preparation method according to claim 1, characterized in that, In step (b), the mixing process is carried out by stirring and homogenizing at a speed of 100-300 r / min for 10-30 minutes.

6. The preparation method according to claim 1, characterized in that, The bees mentioned in step (c) are Italian honeybees or Chinese honeybees.

7. The preparation method according to claim 1, characterized in that, During the secondary brewing process in step (c), the temperature inside the beehive is maintained at 33-36℃ and the relative humidity at 40%-75%.

8. The preparation method according to claim 1, characterized in that, In step (d), honey is harvested by centrifugation, filtration or pressing. Filtration uses a 200-300 mesh filter, and purification is carried out by static precipitation at 4-8℃ for 12-24 hours.

9. The preparation method according to claim 1, characterized in that, In step (d), the harvested honey is also subjected to low-temperature sterilization treatment at a temperature of 40-50℃ for 10-15 minutes.

10. A honey with medicinal value obtained by the preparation method according to any one of claims 1-9.