Application of paromomycin to preparation of medicine for treating trichomonas vaginitis
A technology of trichomonal vaginitis and paromomycin, applied in the field of application of paromomycin in the preparation of medicines for treating trichomonal vaginitis, can solve the problems of drug resistance and side effects, and achieve the goal of solving drug resistance High sex and side effects, inhibition of Trichomonas vaginalis, good killing effect
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experiment example 1
[0044] Experimental example 1 utilizes MTT method to measure the inhibitory action of paromomycin to Trichomonas vaginalis
[0045] 1. Experimental method:
[0046] (1) Culture of Trichomonas vaginalis
[0047] Rinse the cotton swab with trichomonas several times in the culture dish with medium, add antibiotics so that the final concentration of antibiotics is 1000U / ml, and place in CO 2 cultured in an incubator. Replant once every two days, centrifuge at 800rpm / min for 5 minutes; discard the supernatant, add 1-2mL medium to suspend Trichomonas, transfer Trichomonas to 2 petri dishes with appropriate medium, and continue to cultivate the antibiotics gradually Decrease until eventually no antibiotics are added.
[0048] (2) Determination of the inhibitory effect of paromomycin on the growth of Trichomonas
[0049] Inoculate Trichomonas on a 96-well culture plate, 100 μL Trichomonas cell suspension per well, 5000 Trichomonas / well. The above-mentioned 96-well culture plate w...
experiment example 2
[0055] Experimental example 2 Trypan blue staining and counting method to determine the inhibitory effect of paromomycin on trichomonas at different time points
[0056] 1. Experimental method:
[0057] (1) Culture of Trichomonas vaginalis
[0058] Rinse the cotton swab with trichomonas several times in the culture dish added with medium, and add antibiotics so that the final concentration of antibiotics is 1000U / ml. put in CO 2 cultured in an incubator. Replant once every two days, and centrifuge at 800rpm / min for 5 minutes; discard the supernatant, add 1-2mL medium to suspend Trichomonas, transfer Trichomonas to 2 petri dishes with appropriate medium, continue to cultivate and gradually increase the amount of antibiotics Decrease until eventually no antibiotics are added.
[0059] (2) Trypan blue staining and counting
[0060] Spread 24-well plates. Trichomonas were inoculated on 24-well culture plates, 500 μL of cell suspension per well, 100,000 cells / well. The test ...
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