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Application of paromomycin to preparation of medicine for treating trichomonas vaginitis

A technology of trichomonal vaginitis and paromomycin, applied in the field of application of paromomycin in the preparation of medicines for treating trichomonal vaginitis, can solve the problems of drug resistance and side effects, and achieve the goal of solving drug resistance High sex and side effects, inhibition of Trichomonas vaginalis, good killing effect

Inactive Publication Date: 2016-07-06
杨诚 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the present invention aims to propose a use of paromomycin, especially the application in the preparation of medicines for treating trichomonas vaginitis, so as to solve the problem of drug resistance and large side effects of existing medicines. question

Method used

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  • Application of paromomycin to preparation of medicine for treating trichomonas vaginitis
  • Application of paromomycin to preparation of medicine for treating trichomonas vaginitis
  • Application of paromomycin to preparation of medicine for treating trichomonas vaginitis

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0044] Experimental example 1 utilizes MTT method to measure the inhibitory action of paromomycin to Trichomonas vaginalis

[0045] 1. Experimental method:

[0046] (1) Culture of Trichomonas vaginalis

[0047] Rinse the cotton swab with trichomonas several times in the culture dish with medium, add antibiotics so that the final concentration of antibiotics is 1000U / ml, and place in CO 2 cultured in an incubator. Replant once every two days, centrifuge at 800rpm / min for 5 minutes; discard the supernatant, add 1-2mL medium to suspend Trichomonas, transfer Trichomonas to 2 petri dishes with appropriate medium, and continue to cultivate the antibiotics gradually Decrease until eventually no antibiotics are added.

[0048] (2) Determination of the inhibitory effect of paromomycin on the growth of Trichomonas

[0049] Inoculate Trichomonas on a 96-well culture plate, 100 μL Trichomonas cell suspension per well, 5000 Trichomonas / well. The above-mentioned 96-well culture plate w...

experiment example 2

[0055] Experimental example 2 Trypan blue staining and counting method to determine the inhibitory effect of paromomycin on trichomonas at different time points

[0056] 1. Experimental method:

[0057] (1) Culture of Trichomonas vaginalis

[0058] Rinse the cotton swab with trichomonas several times in the culture dish added with medium, and add antibiotics so that the final concentration of antibiotics is 1000U / ml. put in CO 2 cultured in an incubator. Replant once every two days, and centrifuge at 800rpm / min for 5 minutes; discard the supernatant, add 1-2mL medium to suspend Trichomonas, transfer Trichomonas to 2 petri dishes with appropriate medium, continue to cultivate and gradually increase the amount of antibiotics Decrease until eventually no antibiotics are added.

[0059] (2) Trypan blue staining and counting

[0060] Spread 24-well plates. Trichomonas were inoculated on 24-well culture plates, 500 μL of cell suspension per well, 100,000 cells / well. The test ...

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Abstract

The invention provides novel application of paromomycin to preparation of medicine for treating trichomonas vaginitis.The application of paromomycin to preparation of medicine for treating trichomonas vaginitis is implemented by inhibiting trichomonas vaginalis.Compared with the prior art, the application of paromomycin to preparation of medicine for treating trichomonas vaginitis has the advantages that paromomycin of low concentration has a good killing action on trichomonas vaginitis, has an obvious effect on inhibition of trichomonas vaginitis and can be used for preparing medicine for treating trichomonas vaginitis so as to solve the problems of drug resistance and high side effect existing in an existing drug.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry, and in particular relates to the application of paromomycin in the preparation of medicines for treating trichomonas vaginitis. Background technique [0002] Trichomonal vaginitis (TV) is an inflammation of the female reproductive tract caused by Trichomonas vaginalis, and is one of the most common sexually transmitted diseases. According to the WHO report, 180 million people worldwide are infected with TV every year. In addition to mainly causing vaginitis in women, TV infection can also cause cervicitis, urethritis, and infertility. TV is also associated with many perinatal complications, causing premature rupture of membranes, premature birth, and low birth weight infants. TV infection can also cause Trichomonas vaginalis in men (including nongonococcal urethritis, prostatitis, etc.). TV infection is also a risk factor for other sexually transmitted diseases. In recent years, studies hav...

Claims

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Application Information

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IPC IPC(8): A61K31/7036A61P15/02A61P33/02
CPCA61K31/7036
Inventor 杨诚周红刚孙涛
Owner 杨诚
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