48 results about "Trichomonas gallinae" patented technology

A region of the Trichomonas vaginalis AP65-1 gene has been identified which is useful for performing amplification assays to determine specifically whether T. vaginalis is present in the sample being tested. Oligonucleotides useful for performing thermal Strand Displacement Assay (tSDA) reactions on this gene are disclosed. The disclosed oligonucleotides can be used in an assay which is specific for multiple strains of T. vaginalis and which does not show cross reactivity with the genomes of other microorganisms or with human DNA.

The invention relates to the technical field of in vitro diagnosis, and concretely relates to a multiple-detection kit for vaginitis. The kit comprises a kit body and a kit cover, a detection card and a sample processing solution bottle are arranged in kit body, the detection card comprises a card cover and a card body, a Gardnerell avaginalis test strip, a Candida albicans test strip and a Trichomonas vaginalis test strip are arranged in the card body, and each of the Gardnerell avaginalis test strip, the Candida albicans test strip and the Trichomonas vaginalis test strip is provided with a first sample pad layer and a second sample pad layer treated with the sample pad treatment fluid. The kit can decompose and filter out interfering substances in vaginal secretions, so the sensitivity of the kit is improved.

The invention provides a method for treating and / or preventing a wide scope of vaginal and vulval infections, such as those caused by bacteria, parasites or yeasts, by administering gallic acid to a subject in need of treatment. A composition containing gallic acid for treating vaginal infections is also disclosed. It has been found that gallic acid is capable of selectively inhibiting the growth of Trichomonas vaginalis, a parasite that causes trichomonas vaginitis; Gardnerella vaginalis, a bacterium that causes bacterial vaginosis; and Candida albicans, a yeast that causes candidiasis and vulvitis; while not inhibiting the growth of Lactobacillus acidophilus, the dominant bacteria in a healthy vaginal ecosystem. Gallic acid is safe and cost-effective, and can be used alone or incorporated into different vaginal health products to treat and / or prevent vaginal infections.

The invention discloses a Chinese medicinal composition for resisting trichomonas columbae disease. The Chinese medicinal composition comprises the following components by weight portion: 30 to 50 portions of houttuynia, 30 to 50 portions of fried baikal skullcap root, 25 to 40 portions of madder root, 25 to 40 portions of purple gromwell root, 15 to 30 portions of schizonepta, and 15 to 30 portions of bupleurum root. The invention also discloses a method for preparing an oral liquid of the Chinese medicinal composition. In the method, traditional Chinese medicaments are used to replace Western medicaments such as metronidazole, which are prohibited from being used for animal products, to facilitate the production of pollution-free foods. When a pigeon infects the trichomonas columbae disease, the pigeon can be better treated if taking the Chinese medicinal composition and the curative ratio is 93.33 percent, and the effectiveness ratio is 100 percent.

The invention discloses a traditional Chinese medicinal prescription for preventing and treating trichomonad of pigeon, as well as a preparation method and application thereof. The traditional Chinese medicinal prescription comprises the following traditional Chinese medicines in parts by weight: 40-60 parts of fructus cnidii, 40-60 parts of sweet wormwood, 40-60 parts of fructus quisqualis, 30-50 parts of sophora flavescens, 30-50 parts of stemona, 20-40 parts of divaricate saposhnikovia root, 20-40 parts of semen arecae, 10-30 parts of common carpesium fruit, and 10-30 parts of desmodium triquetrum. The traditional Chinese medicinal prescription for preventing and treating trichomonad of pigeon can play the effects of traditional Chinese medicines from multiple aspects, can be used for strengthening the body immunity and improving the laying performance of pigeons, can be used for preventing and treating trichomonad of pigeon, and has an important application value to development of green animal husbandry and promotion of yield improvement of pigeon products.

The invention relates to the technical field of detection of pathogenic microorganisms, in particular to a primer and probe set and kit for multi-detection of gardnerella vaginalis, canidia albicans and trichomonas vaginalis and a detection method. The kit comprises specific primer pairs and Taqman fluorescence probes for conservative areas of GD / CA / TV, and the kit can accurately detect samples infected with GD / CA / TV through real-time fluorescence quantification PCR and is high in sensitivity, good in specificity, high in repeatability and convenient and quick to use.

The use of levo-ornidazole in the preparation of anti-parasitic infection drug is provided. It is demonstrated that levo-ornidazole is superior to dextro-ornidazole and racemic ornidazole in the therapeutic action against parasitization (especially trichomonas vaginalis infection and cecal amoeba infection), and thus it is more practicable to formulate L-ornidazole as anti-parasitic infection drugs, and particularly as drug preparations which are suitable for clinical uses, including oral preparation, intravenous preparation and vaginal preparation.

The present invention provides an application of levomorpholinonidazol [R-(-)-1-(2-methyl-5nitroimidazole-1-group)-morpholinylpropane-2-alcohol] or its medicinal salt in preparation of medicine for resisting amoebic infection and resisting vaginal trichomonad infection.

The present invention provides methods, primers, probes, and kits for identifying gene mutation or material genotyping. In one embodiment, the materials are disease-causing agents. In another embodiment, the present invention is applied to detecting multidrug-resistant mycobacterium tuberculosis, hepatitis B virus, beta-globulin mutation, thrombosis-related mutations; or multiple sexually transmitted diseases causing agents, including but not limited to, trichomonas vaginalis, chlamydia, neisseria gonorrhoeae, mycoplasma, mycoplasma hominis, ureaplasma urealyticum, Mycelia, Treponema pallidum, Herpes simplex virus type 1 and type 2 and human papilloma virus type 6 and type 11.

The invention provides novel application of paromomycin to preparation of medicine for treating trichomonas vaginitis.The application of paromomycin to preparation of medicine for treating trichomonas vaginitis is implemented by inhibiting trichomonas vaginalis.Compared with the prior art, the application of paromomycin to preparation of medicine for treating trichomonas vaginitis has the advantages that paromomycin of low concentration has a good killing action on trichomonas vaginitis, has an obvious effect on inhibition of trichomonas vaginitis and can be used for preparing medicine for treating trichomonas vaginitis so as to solve the problems of drug resistance and high side effect existing in an existing drug.

The invention discloses a nucleic acid molecule, a PCR primer pair and a kit for detecting a beta-tubulin gene of trichomonad. Through extensive research, the inventor clones in trichomonad to obtain a trichomonad beta-tubulin full-length gene coding sequence as shown in SEQ ID NO: 1, and further designs, by utilizing the beta-tubulin full-length gene coding sequence, a PCR primer pair capable of detecting the trichomonad beta-tubulin gene and the transcriptional level of the trichomonad beta-tubulin gene and corresponding PCR reaction conditions; and further, the kit for detecting the beta-tubulin gene of the trichomonad is assembled. According to the invention, the detection requirements of parasitic biology and life science researchers can be met, the gene level detection of the pathogen and the research progress of the pathogenic mechanism of the pathogen can be promoted, and favorable technical support is provided for the research work of gene function research, drug development and the like.

The invention provides a preparation method of trichomonas pigeon and candida albicans bivalent egg yolk antibody powder and an application thereof. The preparation method comprises the following steps: obtaining trichomonas pigeon and candida albicans bacterial liquid; inactivating trichomonas to obtain an inactivated trichomonas antigen, and inactivating candida albicans to obtain an inactivatedcandida albicans antigen; preparing a bivalent inactivated vaccine from the two inactivated antigens and a glucan adjuvant, and immunizing egg laying hens; collecting immunized eggs, separating egg yolk, removing fat components in the egg yolk by using poloxamer, concentrating filtrate by using a hollow fiber ultrafiltration membrane to obtain a concentrated antibody, adding maltodextrin, and carrying out low-temperature freeze drying to obtain the trichomonas pigeon and candida albicans bivalent egg yolk antibody powder. The titer of the egg yolk antibody powder is far higher than the binding titer of any single egg yolk antibody, the egg yolk antibody powder has a remarkable effect of preventing and treating trichomonad and candida albicans infection, and oral ulcer and craw ulcer caused by infection of homing pigeons, racing pigeons, meat pigeons, pearl trichomonad and candida albicans can be effectively prevented and treated by feeding the egg yolk antibody powder.

A method and kit for detecting Trichomonas vaginalis infection in a human subject are disclosed. In the method, a body-fluid sample such as a vaginal-swab sample or urine is obtained from the subject and contacted with an antibody specific against a Trichomonas adhesin peptide, forming an antibody-adhesin peptide complex if the subject is infected with Trichomonas. The presence or absence of the complex establishes, with a reliability of at least 80%, in the case of a vaginal swab sample, and with a reliability of at least 40% in the case of a urine sample, the presence or absence, respectively, of Trichomonas infection in the subject. A preferred test kit employs a dry-strip, sandwich assay, format.

The present invention is directed to the discovery of single nucleotide polymorphisms (SNPs) in the presence of metronidazole-resistant Trichomonas vaginalis. The presence of G76C, C213G, or C318A (SNP) in tvntr 4 or the presence of A238T, G427C, or T476C (SNP) in tvntr6 provides a reliable biomarker for the presence of metronidazole-resistant Trichomonas vaginalis. The present invention further provides reagents used for detecting the SNPs to screen subjects for metronidazole resistance in Trichomonas vaginalis.

The present invention is directed to the discovery of single nucleotide polymorphisms (SNPs) in the presence of metronidazole-resistant Trichomonas vaginalis. The presence of G76C, C213G, or C318A (SNP) in tvntr 4 or the presence of A238T, G427C, or T476C (SNP) in tvntr6 provides a reliable biomarker for the presence of metronidazole-resistant Trichomonas vaginalis. The present invention further provides reagents used for detecting the SNPs to screen subjects for metronidazole resistance in Trichomonas vaginalis.

The invention discloses a manufacturing method of a multiplex PCR kit for quickly detecting vaginal pathogenic microorganisms. The manufacturing method comprises the following steps of: firstly, respectively determining specific primer sequences of candida, Gardnerella vaginalis and trichomonad; preparing a nucleic acid extraction kit suitable for the three pathogens of the candida, the Gardnerella vaginalis and the trichomonad, wherein the nucleic acid extraction kit is suitable for vaginal secretion specimens; finding out fluorescent PCR reaction conditions suitable for the three pathogens of the candida, the Gardnerella vaginalis and the trichomonad, and preparing a fluorescent quantitative PCR detection reagent; and finally, assembling into a complete kit. According to the manufacturing method disclosed by the invention, by preparing the multiplex PCR kit suitable for the three pathogens of the candida, the Gardnerella vaginalis and the trichomonad, which one or mixed infection of the three commonly infected pathogenic microorganisms in a vagina can be quickly detected in one PCR reaction system, so that guidance is provided for clinically selecting targeted treatment medicines as early as possible.

The invention provides a specific nest type PCR detection reagent kit for clinical pentatrichomonas hominis infection. Through two times of PCR amplification, the reagent kit enables a detection method to have higher sensitivity, and is used for specific sensitive fast detection of pentatrichomonas hominis infection in dung (or polluted water samples) of people or animals clinically; the reagent kit has favorable specificity, and misjudgment of detection samples is reduced; and the reagent kit has extremely high application value in the respect of prevention, control and early warning of dungpollution.

Methods and compositions for detection of vulvovaginal candidiasis (VVC), trichomoniasis and bacterial vaginosis (BV) are disclosed herein. In some embodiments, the presence or absence of VVC-associated Candida, Trichomonas vaginalis, and a plurality of BV-related bacteria in a sample is determined using multiplex nucleic acid-based testing methods.

A process and composition for controlling fecal hair excretion and trichobezoar formation in animals such as cats and rabbits is provided and includes feeding the animal a composition comprising from about 10 to about 42 wt % crude protein, from about 4 to about 30 wt % fat, from about 1 to about 25 wt % total dietary fiber, and a supplemental fiber source. The supplemental fiber source is present in amounts which provide from about 1 to about 13 weight percent of supplemental total dietary fiber. The animal is maintained on the diet for a sufficient period of time to control fecal hair excretion and trichobezoar formation.

Methods for the rapid detection of the presence or absence of Trichomonas vaginalis (TV) in a biological or non-biological sample are described. The methods can include performing an amplifying step,a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the target TV gene, along with kits are provided for design of the detection of TV.

The invention is directed to methods, kits, and compositions, for amplifying and detecting Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV), and Mycoplasma genitalium (MG) in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes.

The invention discloses a Chinese medicinal composition for resisting trichomonas columbae disease. The Chinese medicinal composition comprises the following components by weight portion: 30 to 50 portions of houttuynia, 30 to 50 portions of fried baikal skullcap root, 25 to 40 portions of madder root, 25 to 40 portions of purple gromwell root, 15 to 30 portions of schizonepta, and 15 to 30 portions of bupleurum root. The invention also discloses a method for preparing an oral liquid of the Chinese medicinal composition. In the method, traditional Chinese medicaments are used to replace Western medicaments such as metronidazole, which are prohibited from being used for animal products, to facilitate the production of pollution-free foods. When a pigeon infects the trichomonas columbae disease, the pigeon can be better treated if taking the Chinese medicinal composition and the curative ratio is 93.33 percent, and the effectiveness ratio is 100 percent.

The invention is directed to methods, kits, and compositions, for amplifying and detecting Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV), and Mycoplasma genitalium (MG) in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes.

The invention discloses a combination of primers and probes for detection of vaginal microecology and a kit. Target gene detection primers and probes of Lactobacillus (LI), Gardnerella vaginalis (GV), Candida (CA) and Trichomonas vaginalis (TV), and the probes were used for melting curve typing. The kit comprises a primer probe combination. Through the combination of specific primers and probes, the specific target genes of the above-mentioned different microorganisms can be effectively amplified and the amplified products can be analyzed by melting curves, and the above-mentioned seven microorganisms can be detected in one tube, which has high specificity, short time consumption, With the advantages of high sensitivity and comprehensive coverage of detection sites, it can provide clinicians with auxiliary diagnostic references and early treatment, and has a wide range of clinical applications.

The invention belongs to the technical field of microorganisms, and particularly relates to pigeon-derived lactobacillus crispatus BL4014 and application thereof. The invention provides a strain of Lactobacillus crispatus, and the preservation number of the Lactobacillus crispatus is CGMCC (China General Microbiological Culture Collection Center) No.21213. The invention also provides a preparation method of the Lactobacillus crispatus. The lactobacillus crispatus can significantly inhibit trichomonad of pigeon, reduce the number of trichomonad of pigeon in the oral cavity, prevent weight reduction of insect-infected pigeons and prevent substantive damage to the liver caused by trichomonad infection, and has good prevention and treatment effects on trichomonad of pigeon.

The invention provides a trichomonas vaginalis fluorescence immunochromatographic assay kit and a preparation method thereof. The trichomonas vaginalis fluorescence immunochromatographic assay kit comprises a detection card, an SD card and a sample diluent, wherein the detection card is composed of a shell and an immunochromatographic test strip, the immunochromatographic test strip is positionedin the shell and comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a sample suction pad are sequentially arranged on the bottom plate along the length direction,an anti-trichomonas vaginalis monoclonal antibody marked by fluorescent microspheres is fixed on the combination pad, a detection T line and a quality control C line are arranged on the nitrocellulose membrane, the T line is coated with a monoclonal antibody paired with the monoclonal antibody marked by the fluorescent microspheres, and the C line is coated with a secondary antibody paired with the monoclonal antibody marked by the fluorescent microspheres. The kit disclosed by the invention is higher in detection sensitivity, accuracy and precision, and has important significance for dynamically monitoring the illness state of a patient, performing prognosis evaluation, detecting recurrence and guiding clinicians to make important medical decisions in time.

The invention discloses a method for inhibiting activity of trichomonas gallinae in vitro by using bile acid. The application for inhibiting activity of trichomonas gallinae in vitro by using bile acid comprises the following steps: culturing purified trichomonas gallinae in vitro by using a TF complete culture medium or a DMEM complete culture medium containing 4% of fetal bovine serum (FBS) and 1% of three antibodies (penicillin, streptomycin and amphotericin), and preparing a trichomonas strain solution with a certain density; and culturing 600 microliters of trichomonas strain liquid and 400 microliters of TF complete culture medium of bile acid with the concentration of 0.001%-0.09% at the constant temperature of 36.5-38 DEG C, and observing the state and the death rate of trichomonas at different times. Results show that the bile acid can damage cell nucleuses of trichomonas and further has an inhibition effect on trichomonas, and 100% inhibition effect on trichomonas gallinae can be achieved under treatment of the bile acid with proper concentration. The application for inhibiting activity of trichomonas gallinae in vitro by using the bile acid has the following advantages that the inhibition effect of the bile acid on the trichomonas gallinae cultured in vitro is the same as that of metronidazole on the trichomonas gallinae cultured in vitro, and the purpose of replacing metronidazole can be achieved, so that metronidazole and dimetronidazole are further replaced in production, and antibiotic-free breeding and green development are realized.

The invention relates to an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method for ultrafast detection of trichomonas vaginalis, which comprises a reaction procedure for designing a pair of specific primers and probes PF1, PR1 and FQQ aiming at 18S ribosome RNA (Ribose Nucleic Acid) sequence of trichomonas vaginalis and carrying out real-time fluorescent quantitative PCR detection on nucleic acid of a sample to be detected by adopting a fluorescent quantitative PCR reaction. The real-time fluorescent quantitative reverse transcription PCR reaction procedure comprises the following steps: carrying out pollution digestion at 37 DEG C for 2 minutes; pre-denaturation is performed at 95 DEG C for 30 s; denaturation is conducted at 95 DEG C for 2 s, annealing extension is conducted at 69 DEG C for 13 s, and 45 cycles are conducted. According to the primer and the probe disclosed by the invention, the extension temperature meets the optimal extension temperature of Taq DNA polymerase, so that the amplification time is effectively shortened. The extension primer is completely matched with a template, and the Tm value reaches about 69 DEG C, so that annealing and extension can be carried out at the same time at 69 DEG C, two-step amplification can be directly adopted from the beginning, the heating and cooling processes are reduced, and the detection time is effectively shortened.