The invention provides a pharmaceutical composition for vaginal external use, which is a preparation prepared from the following raw materials: Sophora sophora, Pinus pine, Phellodendron cork, Ding kudiding, Fructus Cnidii, Borneol and licorice. The invention also provides a preparation method of the pharmaceutical composition and its application in the preparation of antibacterial, anti-inflammatory, anti-trichomonas and antipruritic drugs for topical vaginal administration; experiments show that the drug of the present invention has definite curative effect, clear therapeutic range, and carries The invention is convenient, has a delicate medicinal taste, and provides quality control standards, so that the medicine of the invention is safe, effective, stable and controllable.