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Vaginal microecological detection primer probe combination and kit

A technology for detecting primers and detecting probes, which is applied in the direction of microorganisms, methods based on microorganisms, measurement/inspection of microorganisms, etc., to achieve the effects of improving amplification specificity, improving detection specificity, and reducing recurrence frequency

Active Publication Date: 2022-06-17
BEIJING ORIGIN-POLY BIO-TEC CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no corresponding detection product on the market to meet clinical needs

Method used

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  • Vaginal microecological detection primer probe combination and kit
  • Vaginal microecological detection primer probe combination and kit
  • Vaginal microecological detection primer probe combination and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Determination of cutoff value of vaginal microbial composition

[0023] For Lactobacillus crispatus (LC), Lactobacillus gasseri (LG), Lactobacillus jensenii (LJ), Lactobacillus inerts (LI), Gardnerella vaginalis (GV), Candida (CA) and vaginal hair droplets The known gene sequences of worms (TV) were compared and analyzed to obtain their respective differential gene sequences, and detection primers and probes were designed for the differential gene sequences, and the detection primers and probes of the internal reference gene GAPDH were also added.

[0024] The specific nucleotide sequences of the designed primers are shown in the following table:

[0025] Table 1. Detection primer sequences corresponding to each microbial target gene

[0026]

[0027] Note: F represents forward detection primer, R represents reverse detection primer, G represents internal reference gene GAPDH.

[0028]A sequence of 3-5bp length and complementary pairing with the 3' end is...

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PUM

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Abstract

The invention discloses a combination of primers and probes for detection of vaginal microecology and a kit. Target gene detection primers and probes of Lactobacillus (LI), Gardnerella vaginalis (GV), Candida (CA) and Trichomonas vaginalis (TV), and the probes were used for melting curve typing. The kit comprises a primer probe combination. Through the combination of specific primers and probes, the specific target genes of the above-mentioned different microorganisms can be effectively amplified and the amplified products can be analyzed by melting curves, and the above-mentioned seven microorganisms can be detected in one tube, which has high specificity, short time consumption, With the advantages of high sensitivity and comprehensive coverage of detection sites, it can provide clinicians with auxiliary diagnostic references and early treatment, and has a wide range of clinical applications.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a primer set and a kit for detecting the risk of vaginal microecological balance. Background technique [0002] The human female vaginal environment refers to the micro-ecological environment composed of all the microbial groups living in the female vaginal cavity. Under normal circumstances, a large number of microorganisms live in the vaginal cavity of women, and these microorganisms play an important role in the local microenvironment to maintain the healthy state of the female vagina. Studies have shown that there are significant differences in the vaginal microbiota of women of different ages, and the typical characteristics of women's vaginal flora are low abundance of Lactobacillus, Gardnerella vaginalis, and Prevotella vulgaris before puberty. With the onset of puberty, stimulated by estrogen, the vaginal epithelium thickens again, which facilitates the colonizati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/689C12Q1/6895C12Q1/6893C12Q1/686C12N15/11C12R1/225C12R1/01C12R1/725C12R1/90
CPCC12Q1/689C12Q1/6895C12Q1/6893C12Q1/686C12Q2600/16C12Q2563/107C12Q2545/114C12Q2527/107
Inventor 王林海刘沛
Owner BEIJING ORIGIN-POLY BIO-TEC CO LTD
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