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Pigeon-derived lactobacillus crispatus BL4014 and application thereof

A Lactobacillus crispatus, pigeon hair technology, applied in the field of microorganisms, can solve problems such as the abuse of antibiotics

Active Publication Date: 2022-06-24
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the abuse of antibiotics has brought many problems to large-scale animal breeding and food safety. Banning antibiotics in feed is an inevitable trend to produce green and safe food and reduce the risk of environmental drug-resistant bacteria.

Method used

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  • Pigeon-derived lactobacillus crispatus BL4014 and application thereof
  • Pigeon-derived lactobacillus crispatus BL4014 and application thereof
  • Pigeon-derived lactobacillus crispatus BL4014 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1. Isolation and identification of bacterial strains

[0039] 1. Isolation of strains

[0040]Collect the fresh pigeon milk in the crop of 4-day-old pigeon, weigh 5 g, put it in a centrifuge tube filled with 25 ml of sterile physiological saline, and shake it well to obtain the initial bacterial liquid. Then draw 0.1ml of the initial bacterial solution into a test tube containing 0.9ml of sterile normal saline, the dilution is 10 -1 , repeat the above process, and make a dilution of 10 in turn -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 of diluted bacteria. pick 10 -5 , 10 -6 , 10 -7 For three dilutions, draw 0.1 ml of diluted bacteria droplets on MRS solid medium, and place them in an anaerobic tank at 37 °C for 24 hours, and pick out typical colonies that are flat and round in appearance and look like the sun.

[0041] 2. Screening of strains

[0042] Using the strains of Trichomonas pigeon isolated from the Pigeon Breeding Laboratory of the Institu...

Embodiment 2

[0053] Embodiment 2, the detection of the effect of Lactobacillus crispatus BL4014 inhibiting Trichomonas pigeon in vivo

[0054] 1. Animal test

[0055] 100 7-day-old white-feather king meat pigeons were selected and raised in separate cages in a strictly sterilized environment without Trichomonas pigeon contamination. 100 7-day-old white-feather king meat pigeons that were not infected with Trichomonas were put on the ankle and weighed to calculate the average body weight. Then, the meat pigeons were randomly divided into five groups according to the average weight, with 20 pigeons in each group and 3 pigeons in each group. The animals were kept in cages, and the upper, middle and lower layers were grouped vertically to ensure that the overall weight of each group remained basically the same. The five groups of pigeons were divided into A group (negative control group), B group (positive control group), C group (metronidazole treatment group), D group (BL4014 prevention gro...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to pigeon-derived lactobacillus crispatus BL4014 and application thereof. The invention provides a strain of Lactobacillus crispatus, and the preservation number of the Lactobacillus crispatus is CGMCC (China General Microbiological Culture Collection Center) No.21213. The invention also provides a preparation method of the Lactobacillus crispatus. The lactobacillus crispatus can significantly inhibit trichomonad of pigeon, reduce the number of trichomonad of pigeon in the oral cavity, prevent weight reduction of insect-infected pigeons and prevent substantive damage to the liver caused by trichomonad infection, and has good prevention and treatment effects on trichomonad of pigeon.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a pigeon-derived Lactobacillus crispatus BL4014 and its application. Background technique [0002] In the breeding process of the livestock and poultry industry, when the disease breaks out, the livestock and poultry will cause intestinal dysfunction under the influence of pathogenic bacteria and viruses. The use of antibiotics can prevent and treat the dysfunction caused by many diseases. However, the abuse of antibiotics has brought many problems to animal scale farming and food safety. Banning antibiotics in feed is an inevitable trend to produce green and safe food and reduce the risk of environmental resistant bacteria. According to Announcement No. 194 of the Ministry of Agriculture and Rural Affairs, from July 1, 2020, feed manufacturers will stop producing commercial feed containing growth-promoting drug feed additives. Finding new green and safe feed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P33/02A23K50/75A23K10/18C12R1/225
CPCC12N1/20A61K35/747A61P33/02A23K50/75A23K10/18A23V2400/133Y02P60/87
Inventor 邵玉新王铮李晶杜少华
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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