Manufacturing method of multiplex PCR kit for quickly detecting vaginal pathogenic microorganisms

Abstract

The invention discloses a manufacturing method of a multiplex PCR kit for quickly detecting vaginal pathogenic microorganisms. The manufacturing method comprises the following steps of: firstly, respectively determining specific primer sequences of candida, Gardnerella vaginalis and trichomonad; preparing a nucleic acid extraction kit suitable for the three pathogens of the candida, the Gardnerella vaginalis and the trichomonad, wherein the nucleic acid extraction kit is suitable for vaginal secretion specimens; finding out fluorescent PCR reaction conditions suitable for the three pathogens of the candida, the Gardnerella vaginalis and the trichomonad, and preparing a fluorescent quantitative PCR detection reagent; and finally, assembling into a complete kit. According to the manufacturing method disclosed by the invention, by preparing the multiplex PCR kit suitable for the three pathogens of the candida, the Gardnerella vaginalis and the trichomonad, which one or mixed infection of the three commonly infected pathogenic microorganisms in a vagina can be quickly detected in one PCR reaction system, so that guidance is provided for clinically selecting targeted treatment medicines as early as possible.

Description

technical field [0001] The invention relates to a method for making a multiplex PCR kit for rapidly detecting vaginal pathogenic microorganisms, belonging to the technical field of biomedicine. Background technique [0002] Vaginitis can cause vulvovaginal symptoms, such as itching, burning pain, irritation, and abnormal discharge. Under normal circumstances, there are aerobic and anaerobic bacteria living in the vagina, forming a normal vaginal flora. When the body function declines, or Receiving external influences will cause the ecological balance between the vagina and the flora to break, forming the pathogenic bacteria Mycoplasma, the common ones are Candida, Gardnerella and Trichomonas; currently, Gardnerella (GD), white Candida (CA), Trichomonas vaginalis (Tv) vaginitis, the lack of rapid detection of which of the three pathogenic microorganisms in vaginal infection or mixed infection. Contents of the invention [0003] In view of the above-mentioned technical prob...

AI Technical Summary

Problems solved by technology

[0002] Vaginitis can cause vulvovaginal symptoms, such as itching, burning pain, irritation, and abnormal discharge. Under normal circumstances, there are aerobic and anaerobic bacteria living in the vagina, forming a normal vaginal flora. When the body function declines, or Receiving external influences will cause the ecological balance between the vagina and the flora to break, forming the pathogenic bacteria Mycoplasma, the common ones are Candida, Gardnerella and Trichomonas; currently, Gardnerella (GD), white Candida (CA), Trichomonas vaginalis (Tv) vaginitis, lack of rapid detection of which of the three pathogenic microorganisms in vaginal infection or mixed infection