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Preparation method of trichomonas pigeon and candida albicans bivalent egg yolk antibody powder and application thereof

A technology of Candida albicans and yolk antibodies, applied in the direction of antibodies, chemical instruments and methods, antifungal agents, etc., can solve the problems of anti-biological abuse and no research, and achieve the effects of reducing abuse, facilitating transportation, and good palatability

Active Publication Date: 2021-02-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Except for metronidazole and nystatin for human use, there are currently no specific and effective drugs for the treatment of trichomonas and candida albicans at home and abroad, and there is no research on related content
The prevention and treatment of these two diseases in clinical breeding mainly adopt antibiotics such as metronidazole, tinidazole, nystatin or other drugs for prevention and treatment, resulting in the abuse of antibiotics and the emergence of drug resistance

Method used

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  • Preparation method of trichomonas pigeon and candida albicans bivalent egg yolk antibody powder and application thereof
  • Preparation method of trichomonas pigeon and candida albicans bivalent egg yolk antibody powder and application thereof
  • Preparation method of trichomonas pigeon and candida albicans bivalent egg yolk antibody powder and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The screening of embodiment 1 trichomonas substratum

[0034] Objective: To screen different media to obtain a large amount of Trichomonas, which is convenient for preparing a large amount of antigenic components of Trichomonas.

[0035]1 Reagents and materials: tryptic soybean broth, yeast extract, fetal bovine serum, bovine liver extract powder, peptone, L-cysteine, K 2 HPO 4 , NaCl, and pregnant horse serum can be purchased from Beijing Quanta Hengyi Biotechnology Co., Ltd.

[0036] Penicillin, streptomycin, amphotericin, and ketoconazole: purchased from China National Institutes for Food and Drug Control.

[0037] Trichomonas pigeon: isolated from diseased pigeons, provided by the isolation and identification of the School of Veterinary Medicine, China Agricultural University.

[0038] 2 methods:

[0039] (1) 10% bovine serum basal medium: 10g peptone, 10g bovine liver extract powder, 5g NaCl, 1g K 2 HPO 4 and 1000mL double distilled water, fully dissolved, au...

Embodiment 2

[0053] The preparation of embodiment 2 Trichomonas pigeon, Candida albicans dual inactivated vaccine

[0054] 1 Reagents and materials:

[0055] Trichomonas pigeon: prepared by the optimized method of Example 1 to obtain 2.5 × 10 8 Individual / mL Trichomonas pigeon.

[0056] Cypic 763A adjuvant: purchased from Cypic (Shanghai) Special Chemicals Co., Ltd.

[0057] β-1,3 / 1,6 glucan: content 95%, purchased from Hubei Angel Yeast Co., Ltd.

[0058] Candida albicans: isolated and identified from diseased meat pigeons, preserved and provided by the School of Veterinary Medicine, China Agricultural University.

[0059] 2 methods:

[0060] 2.1 Candida albicans was cultured on a tryptone soybean broth medium at 180rpm for 17 hours on a shaker, and 2×10 8 CFU / mL of Candida albicans solution.

[0061] Take the culture solution of Trichomonas pigeonii and Candida albicans and centrifuge at 6000rpm for 5min, then discard the supernatant and take the precipitate and wash it with normal...

Embodiment 3

[0067] Embodiment 3 Trichomonas pigeon, the detection of Candida albicans dual antibody

[0068] 1 Reagents and materials:

[0069] Trichomonas pigeon antigen: prepared according to the method in Example 1.

[0070] Candida albicans antigen: see embodiment 2 method.

[0071] Trichomonas pigeon and Candida albicans dual inactivated vaccine: see Example 2 for the preparation method.

[0072] Breeding hen: 180 days old, sea blue and white, Hebei Zhuozhou Mufeng Co., Ltd.

[0073] Feed: Breeder feed during peak season, Hebei Zhuozhou Mufeng Co., Ltd.

[0074] 2 methods:

[0075] 2.1 Immunization method: On the first day of the test, immunize with Trichomonas pigeon and Candida albicans dual inactivated vaccine, and then inject subcutaneously in the neck every 14 days at a dose of 0.5 ml / chicken (each 1 mL contains Trichomonas Antigen is 1×10 9 pcs, Candida albicans is 1×10 9 CFU), after the third immunization, the serum was sampled, and the titer was determined by ELISA met...

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Abstract

The invention provides a preparation method of trichomonas pigeon and candida albicans bivalent egg yolk antibody powder and an application thereof. The preparation method comprises the following steps: obtaining trichomonas pigeon and candida albicans bacterial liquid; inactivating trichomonas to obtain an inactivated trichomonas antigen, and inactivating candida albicans to obtain an inactivatedcandida albicans antigen; preparing a bivalent inactivated vaccine from the two inactivated antigens and a glucan adjuvant, and immunizing egg laying hens; collecting immunized eggs, separating egg yolk, removing fat components in the egg yolk by using poloxamer, concentrating filtrate by using a hollow fiber ultrafiltration membrane to obtain a concentrated antibody, adding maltodextrin, and carrying out low-temperature freeze drying to obtain the trichomonas pigeon and candida albicans bivalent egg yolk antibody powder. The titer of the egg yolk antibody powder is far higher than the binding titer of any single egg yolk antibody, the egg yolk antibody powder has a remarkable effect of preventing and treating trichomonad and candida albicans infection, and oral ulcer and craw ulcer caused by infection of homing pigeons, racing pigeons, meat pigeons, pearl trichomonad and candida albicans can be effectively prevented and treated by feeding the egg yolk antibody powder.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to trichomonad pigeonii and candida albicans dual yolk antibody powder and a preparation method thereof. Background technique [0002] Pigeon trichomoniasis, also known as pigeon oral ulcer, also known as "pigeon cockroach", is one of common pigeon diseases. Throat type manifests as rough button-shaped yellow deposits on the mucous membranes of the mouth and throat; wet ulcers are called wet ulcers; cheese-like or scab-like are called dry ulcers. When the umbilicus is infected, a lump forms under the skin, showing caseous or ulcerative lesions; the visceral type causes visceral organs to appear yellow, rough, and clearly bordered caseous lesions, resulting in necrosis of parenchymal organ tissues. Sick birds interfere with feeding due to mouth sores and can cause high mortality in young and growing birds. According to domestic and foreign data reports, pigeons of a...

Claims

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Application Information

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IPC IPC(8): C07K16/20C07K16/14C07K16/02A61K39/395A61P33/02A61P31/10A61P1/02
CPCC07K16/20C07K16/14C07K16/02A61P33/02A61P31/10A61P1/02A61K2039/507
Inventor 何诚王飞张禾蔡淯涵黄中佐严沛洁
Owner CHINA AGRI UNIV
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