Method for preparing paromomycin crude product by membrane separation technology

A paromomycin and membrane separation technology, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of loss of ion exchange resin, difficult filtration of fermentation liquid, unshaped filter cake, etc. The effect of reducing emissions, simple process and short working time

Inactive Publication Date: 2012-04-04
福建和泉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The shortcomings of the current technology are: dynamic adsorption process, even if filter aid is added in the fermentation liquid plate and frame filtration process, the fermentation liquid is still difficult to filter, the filtration speed is slow, the filtration pressure is as high as 0.4-0.6Mpa, the filter cake is not formed, and the feed liquid In the static adsorption process, the ion exchange resin is separated from the residue in the fermentation broth, the loss of the ion exchange resin is serious, the yield is low, and the wastewater pollution is serious.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Using 260L of fermented broth with a ribomycin concentration of 5.5g / L as raw material, a ceramic microfiltration membrane with a molecular weight cut-off of 50,000 is used. The flux is 35L / h·㎡, the operating pressure is 2.5bar, and the operating temperature is 35°C. Under operating conditions, bacteria and residues in the fermentation broth are removed. The permeate adopts a composite ultrafiltration membrane with a molecular weight cut-off of 5000. Under the operating conditions of a membrane flux of 30 L / h m2, an operating pressure of 5.0 bar, and an operating temperature of 30°C, impurities are removed through the ultrafiltration membrane to remove proteins, Polysaccharides and color-forming substances, etc. The ultrafiltration permeate is treated by nanofiltration with a composite membrane with a molecular weight cut-off of 300. Under the operating conditions of a nanofiltration membrane flux of 30 L / h m2, an operating pressure of 10.0 bar, and an operating tempera...

Embodiment 2

[0022] Using 350L of fermented broth with a ribomycin concentration of 5.0g / L as raw material, ceramic microfiltration membrane with a molecular weight cut-off of 50,000 is used. The flux is 40L / h·㎡, the operating pressure is 3.0bar, and the operating temperature is 30°C. Under operating conditions, bacteria and residues in the fermentation broth are removed. The permeate adopts a composite ultrafiltration membrane with a molecular weight cut-off of 5000. Under the operating conditions of a membrane flux of 30 L / h m2, an operating pressure of 5.0 bar, and an operating temperature of 30°C, impurities are removed through the ultrafiltration membrane to remove proteins, Polysaccharides and color-forming substances, etc. The ultrafiltration permeate is treated by nanofiltration with a composite membrane with a molecular weight cut-off of 300. Under the operating conditions of the nanofiltration membrane flux of 30 L / h·㎡, the operating pressure of 8.0 bar, and the operating tempera...

Embodiment 3

[0024] Using 35L of powder mother liquor with ribomycin concentration of 98.0g / L as raw material, diluted with purified water to 350L, ceramic microfiltration membrane with a molecular weight cut-off of 50,000 is used, the flux is 40L h·㎡, and the operating pressure is 3.0bar. The impurities in the mother liquor are removed under the operating condition of an operating temperature of 30°C. The permeate adopts a composite ultrafiltration membrane with a molecular weight cut-off of 5000. Under the operating conditions of a membrane flux of 30 L / h·㎡, an operating pressure of 5.0 bar, and an operating temperature of 30°C, impurities are removed through the ultrafiltration membrane to remove color-forming substances. Wait. The ultrafiltration permeate is treated by nanofiltration with a composite membrane with a molecular weight cut-off of 300. Under the operating conditions of the nanofiltration membrane flux of 30 L / h·㎡, the operating pressure of 10.0 bar, and the operating tempe...

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Abstract

The invention provides a method for preparing a paromomycin crude product by a membrane separation technology. The method comprises the steps of removing impurities from feed liquid in the preparation process of the paromomycin crude product through a microfiltration membrane, decoloring through ultrafiltration, desalting through nanofiltration, enriching, concentrating and drying and particularly comprises the following steps of: performing liquid solid separation on the feed liquid containing the paromomycin through the microfiltration membrane; removing macromolecular impurities such as protein, pigment, polysaccharide and the like from microfiltration permeate through an ultrafiltration membrane; desalting and enriching the collected permeate through a nanofiltration membrane at normal temperature; and concentrating and drying the desalted liquid to obtain the paromomycin crude product. The method is novel in idea, mild in production process condition, green and clean, simple and practicable, short in production period, low in production cost, high in extraction yield and high in practicability, and has large popularization and application value.

Description

technical field [0001] The invention relates to a method for separating and producing aminoglycoside antibiotic ribomycin, in particular to a method for separating and producing ribomycin by using membrane separation technology. Background technique [0002] Ribomycin is a broad-spectrum antibiotic effective against Staphylococcus, Streptococcus, Pneumococcus, Klebsiella pneumoniae, Escherichia coli and some strains of Proteus. Clinically applicable to various serious infections caused by sensitive Enterobacteriaceae bacteria such as Escherichia coli, Klebsiella, Proteus, Shigella, etc., such as pneumonia, sepsis, biliary tract infection and sensitive Gram Respiratory tract, abdominal cavity, thoracic cavity, urinary tract, skin and soft tissue, bone tissue, eye, ear and nose infections caused by negative bacilli. Compared with other aminoglycoside antibiotics such as kanamycin, it has lower toxicity and less toxicity to hearing and kidneys, which is its main advantage. Us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/23C07H1/08C07H15/232
Inventor 陈灼湖杨斌
Owner 福建和泉生物科技有限公司
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