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Gold nanorod immunoprobe, and preparation method and application thereof

A technology of gold nanorods and gold nanoseeds, which is applied in the field of gold nanorod immune probes and its preparation, can solve the problems of sensor development and application constraints, and achieve the effects of good adaptability, high detection sensitivity, and simple operation

Active Publication Date: 2010-12-22
FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In fact, there are many researches on the application of new nanomaterials to biosensors. However, due to the characteristics of the surface and interface effects, small size effects, and quantum size effects of nanomaterials, the development and application of these sensors are restricted. laboratory development stage

Method used

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  • Gold nanorod immunoprobe, and preparation method and application thereof
  • Gold nanorod immunoprobe, and preparation method and application thereof
  • Gold nanorod immunoprobe, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1, Preparation of "gold nanorod-HIgG" immunoprobe and detection of anti-HIgG in Tris buffer

[0034]1. Preparation of "gold nanorod-HIgG" immunoprobe

[0035] Prepare the "gold nanorod-HIgG" immune probe with the method of the present invention, the specific method comprises the following steps:

[0036] (1) Preparation of gold nanoseeds: First, add 0.25mL 0.01mol / L chloroauric acid solution to 7.5mL 0.1mol / L CTAB solution, mix well, then prepare 0.6mL 0.01mol / L freshly, place Sodium borohydride solution in an ice-water bath was added to the above solution, and mixed for 2 minutes by inverting back and forth to obtain a bright brown yellow solution of gold nano-seeds. The gold nano-seeds were placed in a water bath at 25°C and used after 3 hours.

[0037] (2) Preparation and concentration of gold nanorods: Add 4mL 10mmol / L chloroauric acid solution, 0.6mL 10mmol / L silver nitrate solution and 0.64mL 0.01mol / L ascorbic acid solution to 95mL 0.01mol / L To the CTA...

Embodiment 2

[0044] Example 2, Preparation of "Gold Nanorod-Hepatitis B Surface Antibody" Immunoprobe and Detection in Tris Buffer

[0045] 1. Preparation of "Gold Nanorod-HBsAb" Immunoprobe

[0046] Prepare "gold nanorod-hepatitis B surface antibody" immune probe with the method of the present invention, the specific method comprises the following steps:

[0047] (1) Preparation of gold nano-seeds: the same as in Example 1.

[0048] (2) Preparation and concentration of gold nanorods: the same as in Example 1.

[0049] (3) Antibody modification: 0.2 mL of gold nanorod concentrate was added to 1.0 mL of 0.1 mg / mL monoclonal hepatitis B surface antibody aqueous solution, mixed evenly and left to stand for 30 min to obtain gold nanorod-antibody complexes.

[0050] (4) Sealing: Same as Example 1.

[0051] 2. Detection of HBsAg in Tris buffer with "Gold Nanorods-HBsAb" immunoprobe

[0052] (1) Prepare a solution of standard substances of hepatitis B surface antigen (HBsAg) with a series con...

Embodiment 3

[0055] Example 3: Preparation of "Gold Nanorods-HBsAb" Immunoprobe and Interpretation of HBsAg Negative and Positive Sera

[0056] 1. Preparation of "Gold Nanorod-HBsAb" Immunoprobe

[0057] Prepare "gold nanorod-hepatitis B surface antibody" immune probe with the method of the present invention, the specific method comprises the following steps:

[0058] (1) Preparation of gold nano-seeds: the same as in Example 1.

[0059] (2) Preparation and concentration of gold nanorods: the same as in Example 1.

[0060] (3) Antibody modification: the same as in Example 2.

[0061] (4) Sealing: Same as Example 1.

[0062] 2. Interpretation of hepatitis B surface antigen negative and positive sera with "gold nanorods-hepatitis B surface antibody" immunoprobe

[0063] (1) Negative and positive control sera were selected from the hepatitis B surface antigen detection kit (purchased from Yingke Xinchuang (Xiamen) Technology Co., Ltd.) as samples.

[0064] (2) Add negative and positive s...

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Abstract

The invention discloses a gold nanorod immunoprobe applied to various mediums, and a preparation method and application thereof. The gold nanorod immunoprobe is a product obtained by performing the preparation of gold nano seeds, the growth and concentration of a gold nanorod, antibody modification and sealing. The immunoprobe is subjected to specific recognition in various mediums, such as buffer solution, serum, blood plasma or urine, saliva and the like, and is finally qualitatively and quantitatively analyzed by reading the offset of longitudinal plasma absorption peaks of the gold nanorod. When used for detecting corresponding antigens, the immunoprobe has the advantages of simple operation, high detection sensitivity, high specificity, fewer required instruments and devices, and the like. Moreover, as nano composites are well adapted to external mediums by the special modification mode of the immunoprobe, the immunoprobe also has an expanded application range and is possibly popularized to clinical application.

Description

technical field [0001] The invention belongs to the technical field of nanometer immunity, and in particular relates to a gold nanorod immune probe suitable for various media, its preparation method and application. Background technique [0002] Immunological detection plays a very important role in today's diagnostic science, such as early detection of tumors, diagnosis of infectious diseases and immune diseases, etc. The basic principle of immunological detection is the non-covalent binding reaction between antigen and antibody under the action of various forces, and the initial immune reaction is only carried out between antigen and antibody. With the development of related disciplines, especially the rapid progress in the field of materials science has brought new opportunities for the development of inspection science. Through related reactions, either the antigen or the antibody can be connected to the corresponding new nano-marker, and the change of the magnetic, opt...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/531
Inventor 詹林盛王小慧李媛
Owner FIELD OPERATION BLOOD TRANSFUSION INST OF PLA SCI ACAD OF MILITARY
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