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Method for manufacturing plasma chiral ligand sensor for mercury ions

An aptamer sensor, plasma technology, applied in the field of analytical chemistry technology and food safety, to achieve the effect of simple construction

Active Publication Date: 2013-01-16
JIANGNAN UNIV
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Problems solved by technology

The field of using nucleic acid aptamers to assemble plasmonic nanomaterials to generate circular dichroism signals in the visible light region for the detection of heavy metal ions is still blank.

Method used

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  • Method for manufacturing plasma chiral ligand sensor for mercury ions
  • Method for manufacturing plasma chiral ligand sensor for mercury ions
  • Method for manufacturing plasma chiral ligand sensor for mercury ions

Examples

Experimental program
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Embodiment Construction

[0035] (1) Synthesis of gold nanorods

[0036] Gold nanorods with an aspect ratio of about 3.0 were synthesized according to the method of the patent "A Preparation Method of Self-Assembled Materials with Surface-Enhanced Raman Activity" (Patent No.: ZL 201010605799.9). The simple steps are as follows:

[0037] ① Synthesis of seed crystals: Add 0.1 mL of pre-configured 2 g / L tetrachloroauric acid trihydrate to 1 mL of 0.2 M cetyltrimethylammonium bromide solution at 28 °C. At this time, The color of the solution changed from colorless to yellowish brown. Then add 0.12 mL of newly prepared 0.01 M sodium borohydride solution to the above mixed system and stir rapidly for 2 min. The color of the solution changed from yellowish brown to light brown. Put the above-mentioned synthesized seed crystal into a water bath at 28°C and let it stand for 30 minutes to react, so that the sodium borohydride that does not fully participate in the synthesis of the seed crystal is completely c...

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Abstract

The invention provides a method for manufacturing a plasma chiral ligand sensor for mercury ions, and belongs to the technical field of analytical chemistry and the technical field of food safety. The method mainly comprises the following steps of: (1) synthesizing gold nanorods; (2) performing directional nucleic acid functionalization on surfaces of the gold nanorods; (3) constructing the plasma chiral ligand sensor by using the gold nanorods after the surfaces of the gold nanorods are subjected to the directional nucleic acid functionalization; (4) representing the plasma chiral ligand sensor; (5) measuring the specificity of the plasma chiral ligand sensor; and (6) measuring an actual sample of the plasma chiral ligand sensor. Along with increase of Hg2+ concentration, the number of the gold nanorods in a gold nanorods side surface assembly body is increased, and the Mohr ellipticity of the assembly body is also increased. The linear relation between the Mohr ellipticity and the concentration of the mercury ions is set up, and the plasma chiral ligand sensor which is simple and sensitive and is high in specificity is manufactured and is used for detecting the concentration of the mercury ions.

Description

technical field [0001] The invention discloses a method for preparing a mercury ion plasma chiral aptamer sensor, which belongs to the technical field of analytical chemistry and the technical field of food safety. Background technique [0002] Nanomaterials refer to materials whose three-dimensional spatial scale has at least one dimension at the nanometer level. Nanomaterials are a new generation of materials between atomic clusters and macroscopic materials. Since the size of nanomaterials is close to the de Broglie wavelength of electrons, the superconducting coherence wavelength, and the Bohr radius of excitons, electrons are confined to a very small space, and their wave functions are limited. In addition, they have a large surface area. Therefore, nanomaterials show novel optical, electrical, magnetic and chemical properties different from conventional bulk materials. The assembly of nanomaterials will have new optical, electrical, magnetic and catalytic properties ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/19
Inventor 胥传来徐丽广匡华刘丽强朱颖越赵媛胡拥明
Owner JIANGNAN UNIV
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