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Dual-mode optical coding probe and preparation method thereof

An optical encoding, dual-mode technology, applied in the field of nanomaterials, can solve the problems of SERS spectral overlap, the number of spectrally distinguishable codes, and the limited spectral range, etc., to achieve high repeatability, enhanced optical encoding ability, and simple preparation method. Effect

Inactive Publication Date: 2012-07-11
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the influence of spectral overlapping and resonance energy transfer effects, only about three spectrally resolvable codes can actually be prepared.
However, the commonly used Raman markers have similar molecular structures, so that their SERS spectra also overlap to a large extent, and the available spectral range is limited.
These unfavorable factors make the number of spectrally resolvable codes generated solely based on fluorescent signals or SERS signal coding methods far less than theoretically expected.

Method used

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  • Dual-mode optical coding probe and preparation method thereof
  • Dual-mode optical coding probe and preparation method thereof
  • Dual-mode optical coding probe and preparation method thereof

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preparation example Construction

[0029] The preparation method of the above-mentioned dual-mode optically encoded probe comprises the following steps:

[0030] Step 1. Prepare the original gold nanorod solution: firstly mix the hexadecyltrimethylammonium bromide solution and the tetrachloroauric acid solution, then add the sodium borohydride solution and stir evenly to obtain the gold seed solution; Add silver nitrate solution, tetrachloroauric acid solution, deionized water, and ascorbic acid solution to the hexaalkyltrimethylammonium bromide solution in turn until the mixed solution becomes colorless to make a growth solution; finally add gold to the growth solution. Seed solution to obtain the original gold nanorod solution;

[0031] Step 2. Preparation of Raman molecularly labeled gold nanorods: After the original gold nanorod solution prepared in step 1 is centrifuged with a centrifuge, a precipitate is formed in the original gold nanorod solution, and the precipitate is extracted and added to deionized ...

Embodiment 1

[0035] Using gold nanorods as SERS-enhanced substrates, 5,5-dithiobis(2-nitrobenzoic acid) (DTNB) molecules as SERS markers, and cadmium telluride quantum dots as fluorescent materials to prepare dual-mode optical codes Probe particle, preparation method comprises the steps:

[0036] Step 1. Prepare the original gold nanorod solution: first prepare the gold seeds, at room temperature (that is, in the temperature range of 23~30 °C, the room temperature mentioned below is the same as this), 2.5mL 0.2M hexadecyltri Mix methyl ammonium bromide (abbreviated as CTAB) solution with 1.5mL 1.0mM tetrachloroauric acid solution, stir vigorously and add 0.6mL 0.01M ice-cold sodium borohydride solution, stop stirring after 2 minutes to obtain brown-yellow seed solution. Then prepare the growth solution, add the following reagents in sequence to 50mL 0.2M CTAB solution at room temperature: 2~4mL 4mM silver nitrate solution, 5mL 15mM tetrachloroauric acid solution, 45mL deionized water, and ...

Embodiment 2

[0042] Using gold nanorods as SERS enhanced substrates, using 4-mercaptobenzoic acid (4MBA) molecules as SERS markers, and using cadmium telluride quantum dots as fluorescent materials to prepare dual-mode optically encoded probe particles, the method includes the following steps:

[0043] Step 1: Prepare the original gold nanorod solution. The original gold nanorod solution was prepared according to the dosage and steps in Example 1.

[0044] Step 2: Attach 4MBA molecules to the surface of gold nanorods. Take 5 mL of the original gold nanorod solution and centrifuge once at 10,000 rpm for 30 minutes to remove excess reactants. Disperse the centrifugal precipitate into 5mL deionized water, add 10~50 μL 10mM 4MBA ethanol solution, and stir vigorously for more than 3h.

[0045] Step 3: the surface of the gold nanorods connected with 4MBA molecules is coated with silicon dioxide, and a solution of metal-mediated composite nanospheres is prepared. Centrifuge the solution prepar...

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Abstract

The invention discloses a dual-mode optical coding probe and a preparation method thereof. The probe is in a three-layer core-shell structure, the first-layer core is a gold nano bar, the second-layer shell is silica, the third-layer shell is a cadmium telluride quantum dot, the second-layer shell is wrapped on the outer side of the first-layer core, the third-layer shell covers the outer side ofthe second-layer shell in a sticking mode, and the outer surface of the first-layer core is stuck with Raman molecules which are wrapped by the second-layer shell. The preparation method of the dual-mode optical coding probe comprises the following steps of: 1, preparing an original gold nano bar solution; 2, preparing gold nano bars marked by the Raman molecules; 3, preparing a gold nano bar andsilica metal medium composite nano ball solution; and 4, preparing the dual-mode optical coding probe. The dual-mode optical coding probe has the joint encoding capacity of fluorescence and SERS (Surface Enhanced Raman Scattering), and enhances the optical coding capacity. The preparation method of the dual-mode optical coding probe has a simple process and high repeatability.

Description

technical field [0001] The invention relates to the field of nanomaterials, in particular to a dual-mode optical coding probe and a preparation method thereof. The dual-mode optical coding probe has the functions of joint coding of fluorescence and surface-enhanced Raman scattering. Background technique [0002] With the continuous improvement of preparation technology and characterization methods, optical probes based on nanomaterials have gradually become the focus of researchers at home and abroad because of their unique optical properties and small size. These optical probes with various functions have great application prospects in biosensing, bioimaging and biodetection. [0003] Fluorescence detection technology is fast and simple, and it is a commonly used biological detection method. Traditional organic dye molecules (fluorophores) have inherent disadvantages, such as a wide range of emission spectra and easy bleaching of fluorescence. The semiconductor quantum do...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/88C09K11/02G01N21/65G01N21/64
Inventor 王著元崔一平宗慎飞钟嫄
Owner SOUTHEAST UNIV
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